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Kim, Ok-Tae,Hyun, Dong-Yun,Bang, Kyong-Hwan,Jung, Su-Jin,Kim, Young-Chang,Shin, Yu-Su,Kim, Dong-Hwi,Kim, Swon-Won,Seong, Nak-Sul,Cha, Seon-Woo,Park, Hee-Woon The Korean Society of Medicinal Crop Science 2007 한국약용작물학회지 Vol.15 No.2
The cost of conventional cultivation of ginseng (Panax ginseng C.A. Meyer) is very expensive, because shadow condition should be maintained during cultivation periods owing to inherently weak plant for high-temperature. Therefore, application of plant biotechnology may be possible to overcome these difficulties caused by conventional breeding of ginseng. Transgenic plants were produced via Agrobacterium tumefaciens Gv3101, both carrying the binary plasmid pBI121 mLPISO with nptII and Iso (isoprene synthase) gene. Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using nptII primers and Iso primers. RT-PCR result also demonstrated the foreign isoprene synthase gene in three transgenic plant lines (T1, T3, and T5) which was expressed at the transcriptional level. When whole plants of transgenic ginseng were exposed to high temperature at $46^{\circ}C$ for 1 h, a non-transformed plant was wilted from heat shock, whereas a transgenic plant appeared to remain healthy. We suggest that the introduction of exogenous isoprene synthase is considered as alternative methods far generating thermotolerance ginseng.
Ok-Tae Kim,Dong-Yun Hyun,Kyong-Hwan Bang,Su-Jin Jung,Young-Chang Kim,Yu-Su Shin,Dong-Hwi Kim,Swon-Won Kim,Nak-Sul Seong,Seon-Woo Cha,Hee-Woon Park 한국약용작물학회 2007 한국약용작물학회지 Vol.15 No.2
The cost of conventional cultivation of ginseng (Panax ginseng C.A. Meyer) is very expensive, because shadow condition should be maintained during cultivation periods owing to inherently weak plant for high-temperature. Therefore, application of plant biotechnology may be possible to overcome these difficulties caused by conventional breeding of ginseng. Transgenic plants were produced via Agrobacterium tumefaciens Gv3101, both carrying the binary plasmid pBI121 mLPISO with nptII and Iso (isoprene synthase) gene. Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using nptII primers and Iso primers. RT-PCR result also demonstrated the foreign isoprene synthase gene in three transgenic plant lines (T1, T3, and T5) which was expressed at the transcriptional level. When whole plants of transgenic ginseng were exposed to high temperature at 46℃ for 1 h, a non-transformed plant was wilted from heat shock, whereas a transgenic plant appeared to remain healthy. We suggest that the introduction of exogenous isoprene synthase is considered as alternative methods far generating thermotolerance ginseng.
이종원,김명재,우정택,임천규,배종갑,권오선 대한내과학회 1986 대한내과학회지 Vol.30 No.2
The present study was done to evaluate the clinical features in 69 cases of IgA nephropathy from January 1981 to April l985 and also follow-up renal function was evaluated in 36 cases that were followed up for 22 months retrospectively. The result was summarized as follows; 1) IgA nephropathy was common in young adults and mean age of cases was 30.9 years and male to female ratio was 1.4:l. 2) In clinical manifestation, gross hematuria was in 32%, asymptomatic urinary abnormality in 46%, nephrotic syndrome in 20% and renal insufficiency in 19%. 3) We have observed deterioration of renal function in 11 cases(31%), and 6 cases(17%) were progressed in deterioration of renal function in follow-up study. 4) Nephrotic range proteinuria and hypertension were closely associated with poor prognosis and other prognostic factors were not significant statistically.