Melatonin attenuates cisplatin-induced acute kidney injury in rats via induction of anti-aging protein, Klotho

Ko, Je-Won,Shin, Na-Rae,Jung, Tae-Yang,Shin, In-Sik,Moon, Changjong,Kim, Sung-Ho,Lee, In-Chul,Kim, Sung-Hwan,Yun, Won-Kee,Kim, Hyoung-Chin,Kim, Jong-Choon Elsevier 2019 Food and chemical toxicology Vol.129 No.-

<P><B>Abstract</B></P> <P>This study investigated the protective effects of melatonin (MT) against cisplatin (CP)-induced acute kidney injury in rats as well as its possible mechanism of action associated with anti-aging protein Klotho. The following four experimental groups were evaluated: vehicle control, CP (7 mg/kg), CP&MT20 (20 mg/kg/day), and CP&MT40 (40 mg/kg/day). The concomitant administration of MT significantly ameliorated CP-induced acute kidney injury in rats, as evidenced by increased kidney weight, increased serum levels of blood urea nitrogen and creatinine, and increased incidence of histopathological alterations with renal tubular cell apoptosis. In addition, MT treatment protected kidney tissue against oxidative damages and significantly upregulated the expression level of Klotho decreased by CP treatment, resulting in reduced phosphorylation of protein kinase B (AKT) and forkhead box O (FOXO) as well as reduced expression levels of B-cell lymphoma 2-associated X protein (Bax) and caspase-3. MT not only partially regulated oxidative stress via AKT/FOXO signaling, but also reduced apoptosis caused by CP by inhibiting the Bax/caspase-3 pathway. Our results indicated that MT could prevent acute kidney injury induced by CP in rats, presumably through upregulating the expression of Klotho, resulting in elevated anti-oxidant and anti-apoptotic properties.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Melatonin (MT) attenuated acute kidney injury induced by cisplatin (CP). </LI> <LI> MT upregulated the expression level of Klotho decreased by CP treatment. </LI> <LI> Upregulated Klotho attenuated oxidative stress and apoptosis induced by CP. </LI> </UL> </P>

Development of a Type 4 Composite Cylinder for Self-contained Breathing Apparatus

Sung-min Cho(조성민),Da-eun Kim(김다은),Hye-jin Seong(성혜진),Young-kyu Ko(고영규),Hong-chul Kim(김홍철),Kang-ok Lee(이강옥),Min-sik Jo(조민식),Sung-ki Lyu(류성기) 한국기계가공학회 2019 한국기계가공학회지 Vol.18 No.12

Aluminum liners used in cylinders are hazardous for human health. In this study, we use a plastic PA liner inside cylinders to solve this problem. Plastic PA liners are widely used in the manufacturing industry in the production of food and beverage containers. We covered the aluminum boss with a plastic liner material and wound the composite fibers over the liner material. To reinforce the dome area, we used low strength / high elongation plastic liner. To predict the performance of the developed product, we conducted structural analyses utilizing the 3D laminated solid element. We verified the soundness of the product by testing the prototype.

EMA-CO regimen in a patient with cisplatin-refractory primary retroperitoneal choriocarcinoma

( Sang Uk Ko ),( Ji Na Yun ),( Se Hyung Kim ),( Hyun Jung Kim ),( Chan Kyu Kim ),( Seong Kyu Park ),( Dae Sik Hong ) 대한내과학회 2013 대한내과학회 추계학술발표논문집 Vol.2013 No.1

Primary retroperitoneal choriocarcinoma is an uncommon extragonadal germ cell tumor that generally has a very poor prognosis because of early hematogenous and lymphatic metastatic spread and inherent resistance to anticancer drugs. We experienced a case of cisplatin-refractory primary retroperitoneal choriocarcinoma treated successfully with a second-line regimen of etoposide, methotrexate, actinomycin D, cyclophosphamide and vincristine (EMA-CO), followed by residual surgical resection. A 32-year-old man presented with a huge palpable mass in the left upper quadrant (LUQ). He had a 1-month history of intermittent dyspepsia and LUQ pain. Computed tomography showed a 16×11-cm retroperitoneal mass and multiple liver and lung metastases. A heterogenous mass in the left testis was found on ultrasonography. However, the orchiectomy showed a subalbugineal fibrous scar close to the rete testis. We regarded this as a “burned-out” phenomenon. A liver biopsy revealed pure choriocarcinoma. Furthermore, the human chorionic gonadotropin β-subunit was elevated at 317,449 ng/ml, while the α-fetoprotein was normal, resulting in a diagnosis of primary retroperitoneal choriocarcinoma. He was given bleomycin, etoposide, and cisplatin (BEP) chemotherapy. After two chemotherapy cycles, the response evaluation revealed progressive disease. We used EMA-CO as a second-line regimen, as it is the regimen used most widely for gestational trophoblastic tumors. After eight cycles, the patient attained a potentially resectable state. We surgically removed all of the residual masses in the liver and retroperitoneum. Histologically, these proved to be necrotic fibrous tissue. He has remained disease-free 20 months after surgery without additional therapy. An EMA-CO regimen is a reasonable therapeutic option for cisplatin-refractory primary retroperitoneal choriocarcinoma.

Diallyl Disulfide Prevents Cyclophosphamide-Induced Hemorrhagic Cystitis in Rats through the Inhibition of Oxidative Damage, MAPKs, and NF-κB Pathways

( Sung Hwan Kim ),( In Chul Lee ),( Je Won Ko ),( Chang Jong Moon ),( Sung Ho Kim ),( In Sik Shin ),( Young Won Seo ),( Hyoung Chin Kim ),( Jong Choon Kim ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.2

This study investigated the possible effects and molecular mechanisms of diallyl disulfide (DADS) against cyclophosphamide (CP)-induced hemorrhagic cystitis (HC) in rats. Inflammation response was assessed by histopathology and serum cytokines levels. We determined the protein expressions of nuclear transcription factor kappa-B (NF-κB), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumor necrosis factor-α (TNF-α), oxidative stress, urinary nitrite-nitrate, malondialdehyde (MDA), and 8-hydroxy-2’-deoxyguanosine (8-OHdG). Finally, we studied the involvement of mitogen-activated protein kinases (MAPKs) signaling in the protective effects of DADS against CP-induced HC. CP treatment caused a HC which was evidenced by an increase in histopathological changes, proinflammatory cytokines levels, urinary nitrite-nitrate level, and the protein expression of NF-κB, COX-2, iNOS, TNF-α, p-c-Jun N-terminal kinase (JNK), and p-extracellular signal regulated kinase (ERK). The significant decreases in glutathione content and glutathione-S-transferase and glutathione reductase activities, and the significant increase in MDA content and urinary MDA and 8-OHdG levels indicated that CP-induced bladder injury was mediated through oxidative DNA damage. In contrast, DADS pretreatment attenuated CP-induced HC, including histopathological lesion, serum cytokines levels, oxidative damage, and urinary oxidative DNA damage. DADS also caused significantly decreased the protein expressions of NF-κB, COX-2, iNOS, TNF-α, p-JNK, and p-ERK. These results indicate that DADS prevents CP-induced HC and that the protective effects of DADS may be due to its ability to regulate proinflammatory cytokines production by inhibition of NF- κB and MAPKs expressions, and its potent anti-oxidative capability through reduction of oxidative DNA damage in the bladder.

Effects of Lower Limb Functional Exercise in an Unstable Ground on Lower Limb Strength and the Balance Ability

( Sung Sik Ko ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1

Purpose: The purpose of this study was to investigated the effects of lower limb functional exercise in an unstable ground on lower limb strength and the balance ability of encephalopathy teenagers. Method: The lower limb functional exercise was applied once every 50 minutes twice a week during 12 weeks in the experimental group, meanwhile control group did not receive any treatment except daily life. In order to evaluate the lower limb strength and the balance ability of the study subjects from exercise before and after exercise, the lower limb strength test using thera bands, static balance ability test(standing by one leg, FRT) and dynamic balance ability test(TUG, walking 10m, walking 3m) were used. Result: First, the measured results of the lower limb strength using thera bands was that the bending force and new force of a hip joint increased significantly(p=.000), also the bending force and new force of the knee joint increased significantly(p=.000). Second, in the static balance ability, standing by one leg with opened eyes and functional arm stretching(FRT) increased significantly( p=.000, p.002) respectively. Third, in the dynamic balance ability, in standing up and walking(TUG) and in walking straight 3m(Tandem Gait Test) decreased significantly(p=.000, p=.000), in walking straight 10m(Straight Walking Test) appeared no change. Conclusion: These suggest that the lower limb functional exercise in an unstable ground during 12 weeks is believed to improve the lower limb strength and also static and dynamic balance ability of encephalopathy teenagers.

Effects of men-to-men exercise training program on body composition and health-related fitness in university students

( Sung Sik Ko ) 대한운동사협회 2020 대한운동사협회 운동사대회자료집 Vol.2020 No.-

Effects of 60%VO₂R intensity equicaloric intermittent and continuous exercise on EPOC

( Sung Sik Ko ) 대한운동사협회 2017 대한운동사협회 운동사대회자료집 Vol.2017 No.-

Spinosin, a C-Glucosylflavone, from Zizyphus jujuba var. spinosa Ameliorates Ab1-42 Oligomer-Induced Memory Impairment in Mice

( Sang Yoon Ko ),( Hyung Eun Lee ),( Se Jin Park ),( Se Jin Jeon ),( Bo Seong Kim ),( Qing Tao Gao ),( Dae Sik Jang ),( Jong Hoon Ryu ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.2

Alzheimer’s disease (AD) is a neurodegenerative disorder associated with progressive memory loss and neuronal cell death. Although numerous previous studies have been focused on disease progression or reverse pathological symptoms, therapeutic strategies for AD are limited. Alternatively, the identification of traditional herbal medicines or their active compounds has received much attention. The aims of the present study were to characterize the ameliorating effects of spinosin, a C-glucosylflavone isolated from Zizyphus jujuba var. spinosa, on memory impairment or the pathological changes induced through amyloid-b1-42 oligomer (AbO) in mice. Memory impairment was induced by intracerebroventricular injection of AbO (50 μM) and spinosin (5, 10, and 20 mg/kg) was administered for 7 days. In the behavioral tasks, the subchronic administration of spinosin (20 mg/kg, p.o.) significantly ameliorated AbO-induced cognitive impairment in the passive avoidance task or the Y-maze task. To identify the effects of spinosin on the pathological changes induced through AbO, immunohistochemistry and Western blot analyses were performed. Spinosin treatment also reduced the number of activated microglia and astrocytes observed after AbO injection. In addition, spinosin rescued the AbO-induced decrease in choline acetyltransferase expression levels. These results suggest that spinosin ameliorated memory impairment induced through AbO, and these effects were regulated, in part, through neuroprotective activity via the anti-inflammatory effects of spinosin. Therefore, spinosin might be a useful agent against the amyloid b protein-induced cognitive dysfunction observed in AD patients.

Effects of individual aerobic exercise training on body composition, VO₂max, and resting heart rate in university students

( Sung Sik Ko ) 대한운동사협회 2022 대한운동사협회 운동사대회자료집 Vol.2022 No.-

[INTRODUCTION] The purpose of this study was to investigate the effects of the individualized aerobic exercise training program on the body composition, cardiorespiratory endurance, and resting heart rate of male and female university students. [METHOD] A total of 28 subjects, 17 male and 11 female, participated in this study. Subjects performed an individualized exercise program suitable for their physical fitness level in the exercise intensity range of 50-85% VO2max for 40-60 minutes per day, 3-5 times per week for 12 weeks. As for the exercise intensity, the exercise intensity and time were gradually increased at 2-week intervals according to the individual's physical fitness level. Body composition, maximum oxygen intake, and resting heart rate were measured before and after the exercise program. Data were analyzed by two-way ANOVA and statistical significance was determined as α=.05. [RESULT] The weight of males was higher than that of females, and both males and females decreased significantly in the same manner. Fat mass was significantly higher in women than in men, and both men and women significantly decreased in the same pattern. Lean body mass was significantly higher in men than in women, and both men and women tended to slightly increase, but there was no significant change. Body fat percentage was significantly higher in women than in men, and both men and women significantly decreased in the same pattern. The maximum oxygen intake was significantly higher in men than in women, and the increase was higher in women than in men. The resting heart rate was significantly higher in women than in men, and decreased significantly in the same pattern in both men and women. [CONCLUSION] These results suggest that the individualized aerobic exercise program positively improved body composition for both men and women, and the resting heart rate showed the same pattern. However, it is summarized that the maximum oxygen uptake was improved in both men and women, but it was more effective in women.

A relationship between Stroke and Sasang Constitution in Korean

Ko, Ho-Yeon,Jun, Chan-Yong,Park, Jong-Hyeong,Yoon, Yoo-Sik,Lee, Sun-Dong,Han, Chang-Ho,Jung, Woo-Sang,Moon, Sang-Kwan,Cho, Ki-Ho,Ko, Seong-Gyu Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.4

Experts of Sasang Constitution Medicine of Traditional Korean Medicine have classified stroke patients with four types of Sasang constitutions in their clinical practice and some types of Sasang constitutions have been regarded as risk factors of stroke, but this is uncertain because there were no evidences by large scale of prospective studies. The purpose of this was to study the association between strokes and Sasang constitutions. Case-control study has been conducted to the patients admitted to the research hospitals. The patients were confirmed stroke by brain MRI or CT scans and recruited from May 2003 to August 2005. The subjects who met the requirement of inclusion and exclusion criteria were 108 patients as the cases and 107 as healthy controls. Data collection has been performed by the trained specialists majoring neurologists through interviews, physical examinations, and laboratory testes. No statistical significance was obtained between the strokes and Sasang constitutions, yet Taeumin, and Soyangin types showed a trend of increase in the incidence of strokes as compared with Soeumin. To acquire more concrete data on this theme, we need further and large scale of prospective researches.