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Sung Hee Yoon,Sun Ok Yun,Jung Yong Park,Hee Yeun Won,Eun-Kyung Kim,Hyun-Jung Sohn,Hyun-Il Cho,김태규 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.3
Increasing importance is being given to the stimulation of Th1 response in cancer immunotherapy because its presence can shift the direction of adaptive immune responses toward protective immunity. Based on chemokine receptor expression, CXCR3+CCR4-CD4+ T cells as Th1-type cells were investigated its capacity in monocyte-derived dendritic cell (DC) maturation and polarization, and induction of antigen specific cytotoxic T lymphocytes (CTL) in vitro. The levels of IL-4, IL-5 and IL-10 were decreased to the basal level compared with high production of IFN-γ, TNF-α, and IL-2 in CXCR3+CCR4-CD4+ T cells stimulated with anti-CD3 and anti-CD28 antibodies. Co-incubation of activated CD4+ or CXCR3+CCR4-CD4+ T cells with DC (CD4+/DC or CXCR3+CD4+/DC, respectively) particularly up-regulated IL-12 and CD80 expression compared with DC matured with TNF-α and LPS (mDC). Although there was no significant difference between the effects of the CXCR3+CCR4-CD4+ and CD4+ T cells on DC phenotype expression, CXCR3+CD4+/DC in CTL culture were able to expand number of CD8+ T cells and increased frequencies of IFN-γ secreting cells and overall cytolytic activity against tumor antigen WT-1. These results demonstrated that the selective addition of CXCR3+ CCR4- CD4+ T cells to CTL cultures could enhance the induction of CTLs by DC in vitro, and implicated on a novel strategy for adoptive T cell therapy. Increasing importance is being given to the stimulation of Th1 response in cancer immunotherapy because its presence can shift the direction of adaptive immune responses toward protective immunity. Based on chemokine receptor expression, CXCR3+CCR4-CD4+ T cells as Th1-type cells were investigated its capacity in monocyte-derived dendritic cell (DC) maturation and polarization, and induction of antigen specific cytotoxic T lymphocytes (CTL) in vitro. The levels of IL-4, IL-5 and IL-10 were decreased to the basal level compared with high production of IFN-γ, TNF-α, and IL-2 in CXCR3+CCR4-CD4+ T cells stimulated with anti-CD3 and anti-CD28 antibodies. Co-incubation of activated CD4+ or CXCR3+CCR4-CD4+ T cells with DC (CD4+/DC or CXCR3+CD4+/DC, respectively) particularly up-regulated IL-12 and CD80 expression compared with DC matured with TNF-α and LPS (mDC). Although there was no significant difference between the effects of the CXCR3+CCR4-CD4+ and CD4+ T cells on DC phenotype expression, CXCR3+CD4+/DC in CTL culture were able to expand number of CD8+ T cells and increased frequencies of IFN-γ secreting cells and overall cytolytic activity against tumor antigen WT-1. These results demonstrated that the selective addition of CXCR3+ CCR4- CD4+ T cells to CTL cultures could enhance the induction of CTLs by DC in vitro, and implicated on a novel strategy for adoptive T cell therapy.
Cerebral Ischemia Caused by Hepatic Fascioliasis
Sang-Won Park,Sung-Il Sohn 대한뇌졸중학회 2010 Journal of stroke Vol.12 No.1
Fasciola hepatica, a zoonotic liver fluke, can cause disease in humans. Common symptoms and signs of acute fascioliasis are epigastric or upper abdominal pain, fever, arthralgia, and malaise. However, cerebral symptoms of hepatic fascioliasis are very rare. We report a case of cerebral lesions caused by typical fascioliasis that was diagnosed by laboratory and imaging findings.
Biological Effects of Smoking-induced Environmental Toxicity
Sohn, Sung-Hwa,Kim, In-Kyoung,Kim, Ki-Nam,Kim, Hye-Won,Seo, Sang-Hui,Lee, Seung-Ho,Kim, Yu-Ri,Lee, Eun-Il,Kim, Meyoung-Kon The Korean Society of Toxicogenomics and Toxicopro 2006 Molecular & cellular toxicology Vol.2 No.3
Our objective is to identify molecular factors which contribute to the increased risk of smoke in human. About 677 workers who had control and experimental groups according to their urinary Naphthol levels were enrolled in our study. In the present study, we investigated the effects of smoking on gene expression profiles in human. We determined differential gene expression patterns in smoker versus non-smoker using cDNA microarray. Specific genes were up-or down-regulated according to smoking and age. Inflammatory related genes such as cytokine, interleukin, and tumor necrosis factor were up-regulated, DNA repair related genes such as high-mobility group (nonhistone chromosomal) protein 1, and protein 2 were down-regulated, apoptosis related genes such as myeloperoxidase and Bcl-2-associated athanogene were down-regulated, and cell cycle related genes were down-regulated. In our epidemiological study, notably, inflammatory, DNA repair, apoptosis, signal transduction, metabolism, cell cycle, cell proliferation, transcription related genes were regulated.
Vasoconstriction Potency Induced by Aminoamide Local Anesthetics Correlates with Lipid Solubility
Sung, Hui-Jin,Ok, Seong-Ho,Sohn, Jin-Young,Son, Yong Hyeok,Kim, Jun Kyu,Lee, Soo Hee,Han, Jeong Yeol,Lim, Dong Hoon,Shin, Il-Woo,Lee, Heon-Keun,Chung, Young-Kyun,Choi, Mun-Jeoung,Sohn, Ju-Tae Hindawi Publishing Corporation 2012 Journal of biomedicine & biotechnology Vol.2012 No.-
<P>Aminoamide local anesthetics induce vasoconstriction <I>in vivo</I> and <I>in vitro</I>. The goals of this <I>in vitro</I> study were to investigate the potency of local anesthetic-induced vasoconstriction and to identify the physicochemical property (octanol/buffer partition coefficient, pKa, molecular weight, or potency) of local anesthetics that determines their potency in inducing isolated rat aortic ring contraction. Cumulative concentration-response curves to local anesthetics (levobupivacaine, ropivacaine, lidocaine, and mepivacaine) were obtained from isolated rat aorta. Regression analyses were performed to determine the relationship between the reported physicochemical properties of local anesthetics and the local anesthetic concentration that produced 50% (ED<SUB>50</SUB>) of the local anesthetic-induced maximum vasoconstriction. We determined the order of potency (ED<SUB>50</SUB>) of vasoconstriction among local anesthetics to be levobupivacaine > ropivacaine > lidocaine > mepivacaine. The relative importance of the independent variables that affect the vasoconstriction potency is octanol/buffer partition coefficient > potency > pKa > molecular weight. The ED<SUB>50</SUB> in endothelium-denuded aorta negatively correlated with the octanol/buffer partition coefficient of local anesthetics (<I>r</I><SUP>2</SUP> = 0.9563; <I>P</I> < 0.001). The potency of the vasoconstriction in the endothelium-denuded aorta induced by local anesthetics is determined primarily by lipid solubility and, in part, by other physicochemical properties including potency and pKa.</P>
Sohn, Kook Ho,Jo, Mi Jeong,Cho, Won Joon,Lee, Jong Rok,Cho, Il Je,Kim, Sang Chan,Kim, Young Woo,Jee, Seon Young Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-
<P><I>Bojesodok-eum</I> (BSE) is a herbal prescription consisting of <I>Coptidis Rhizoma</I> and <I>Scutellariae Radix</I> as main components. This paper investigated the effects of BSE on the induction of nitric oxide (NO), prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>), and proinflammatory cytokines that are caused by lipopolysaccharide (LPS) in murine macrophage cell line and on the paw edema formation in animals. Administration of BSE (0.3 g/kg and 1 g/kg) in rats significantly inhibited carrageenan-induced paw edema formation, as did dexamethasone, an anti-inflammatory positive control drug. In cell model, treatment of BSE decreased the production of NO and PGE<SUB>2</SUB> in RAW264.7 cells stimulated by LPS. BSE also inhibited the expression of iNOS and COX-2 protein as well as COX activity in a concentration-dependent manner. Consistently, BSE suppressed the ability of LPS to produce TNF-<I>α</I>, interleukin-1<I>β</I>, and interleukin-6. LPS treatment induced nuclear NF-<I>κ</I>B level and I-<I>κ</I>B<I>α</I> phosphorylation, which were inhibited subsequent treatment of BSE, suggesting its repression of LPS-inducible NF-<I>κ</I>B activation. BSE abrogated the induction of NO, PGE<SUB>2</SUB>, and proinflammatory cytokines, as well as iNOS and COX-2 protein expression in RAW264.7 cells stimulated by LPS as mediated with NF-<I>κ</I>B inhibition.</P>
A Case of High-degree Atrioventricular Block in a Patient with Ankylosing Spondylitis
( Sang Rok Lee ),( Hyung Wook Park ),( Sang Yub Lim ),( Seo Na Hong ),( Kye Hun Kim ),( Il Suk Sohn ),( Young Joon Hong ),( Ju Han Kim ),( Weon Kim ),( Young Keun Ahn ),( Myung Ho Jeong ),( Jeong Gwan 대한내과학회 2005 대한내과학회 추계학술대회 Vol.69 No.10
Sang, Jung Ho,Il, Yang Woo,Hun, Lee Jae,Min, Sohn Jae,Nam, Choo Kee,Goo, Kim Bong,Young, Lee Sang Science Press 2010 CHINESE PHYSICS LETTERS Vol.27 No.8
<P>The film thickness should be known for extracting the intrinsic surface resistance from the effective surface resistance as measured by using the dielectric resonator method. Thicknesses of 70 nm to 360 nm are measured for YBa<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>7−δ</SUB> films in a non-invasive way by using the two-resonant-mode dielectric resonator (TDR) method. A rutile resonator with the respective resonant frequencies of 15.25–15.61 GHz and 15.10–15.37 GHz for the TE<SUB>021</SUB> and the TE<SUB>012</SUB> modes is used for this purpose. Differences between the values as measured by using the TDR technique and those measured by using a step profilometer appear to be less than 3%, which is smaller than the previous value of 5% as measured by using a 8.6 GHz single-resonance mode rutile resonator [Lee et al. J. Korean Phys. Soc. 54(2009)1619]. Merits of using the TDR method are discussed.</P>