[논문]Operational Characteristics of CO2 Laser Adopted by Superposing-Discharge

Hee-Je Kim,Hyun-Ju Chung,Byoung-Dae Min,Jong-Han Joung,Sung- lun Park 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

오늘날 현저한 CO2 레이저의 응용 추세에 발맞추어 보다 다양한 형태의 출력 제어 기술이 대두되고 있다. 본연구에서는multi-Pulse Superposing-Discharge 기술을 이용하여 다양한형태의 CO2 레이저의 펄스를 성형 하였다. 펄스 성형을 위한 방법으로 PIC one-chip microprocessor를 이용하여 삼중 PFN 모률의 SCR을 각각 고유의 지연시간을 가지도록 제어하였다. 실험에 사용된 삼중 PFN 모률의 전원 회로는 각각 캐패시터 인덕터,SCR,고압 펄스 트랜스 그리고 고압 트랜스 2차측에 브릿지 정류기로 구성 하였다. 고압 펼스 트랜스의 1차측을 제어하므로서 PFN 모률의 동작을 저 전압에서 수행 할 수 있다. 트랜스의 2차측에 연결된 전파 정류기는 펄스 에너지를 부하에 지속적으로 전달한다. 본 연구에서는 삼중 PFN 모률의 각각의 SCR을 다른 시간 가격을 두고 트리거 하므로서 다양한 Long pulse shaping을 실현하였다. 또한 약 250-1000μs 의 주기를 가지는 다양한 펄스형 레이저 빔을 얻었다.

Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells

Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4

The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.

아동양육시설 퇴소 후 청소년들의 자립생활에 영향을 미치는 요인에 관한 사례연구

한성희 ( Sung Hee¸ Han ) 초록우산 어린이재단(구 한국복지재단) 2020 어린이재단 연구논문 모음집 Vol.2020 No.-

본 연구는 질적 사례연구 방법을 사용하여 시설 퇴소 청소년들의 자립생활에 영향을 미치는 요인들 중 사회제도적 · 공식적 · 비공식적 지원체계에는 어 떠한 것들이 있는가를 파악하고 이들의 기능과 역할 및 그 체계들 간의 상호관련성을 파악하고자 하였다. 이를 위해 퇴소 후 5년 이내인 청소년 2명을 대상으로 심층면접을 진행하였다. 심층면접을 통한 사례를 분석하여 연구결과를 종합 하였으며 그 결과는 다음과 같다. 시설 퇴소 청소년의 자립생활에 다양한 개인 적 환경적 요인들이 작용하고 있으며 이는 비공식적, 사회제도적 자원체계의 영향력 아래에서 유기적으로 작동하고 있음을 확인할 수 있었다. 그러나 이러한 공식적, 사회제도적 자원체계는 개별적으로 영향력을 행사하는 것이 아니라 자립 후 퇴소청소년이 처한 상황에 따라 달라질 수 있으며 결국 이 사회적 자원체계와 비공식적 자원체계가 균형과 조화가 이룰 수 있도록 공식적 자원체계가 그 기능과 역할을 강화하여야 함을 알 수 있었다. 특히 시설퇴소 청소년들의 성공적인 자립생활을 돕기 위해 퇴소한 양육시설과의 연계 없이도 그들에게 정부지원의 자립지원혜택을 정확하게 안내해 주고 이를 잘 활용할 수 있도록 돕는 공식적 자원체계의 기능을 강화해야 한다는 점을 제안하였다. The purpose of this study is to understand social institutional, formal and informal resource systems to affect independent living of adolescents after leaving the child care institution by the method of qualitative case study, and to grasp interrelationship between function, role and system of its factors. To accomplish this purpose, we had in-depth interview with 2 participants who left child care institution within five years and have collected various data by analyzing the cases. The result showed that various personal and environmental factors affect independent living of adolescents after leaving the child care institution, and these factors are operated organically in informal and social institutional resource systems. However, the formal and social institutional resource systems are subject to the condition of adolescents after leaving the care of the Institution. In this study, we have found that the function and roles of formal resource system should be strengthened for achieving balance between these systems. In conclusion, for helping adolescents who left the care of institution to succeed in their independent living, we suggest that the formal resource system should guide the public assistance correctly and help them to use the public service effectively.

혈압점(血壓點)에 윤제(潤劑)의 주입이 고혈압에 미치는 영향

위종성,한재섭,박희수,박재영,Owi, Jong-Sung,Han, Jai-Seop,Park, Hee-Soo,Park, Jai-Young 대한약침학회 2002 Journal of pharmacopuncture Vol.5 No.1

Objective: This study was designed to evaluate the possibility of Hypertension by moistening Herbal-acupuncture at Blood Pressure Point(HN136). Methods: We reviewed 14 patients of Hypertension. They were hospitalized at oriental medical hospital of Sang-Ji university for 2002. 1. 2. - 2002. 5. 2. First, we divided into two groups; Group Ⅰ was administrated by moistening Herbal-acupuncture at Blood Pressure Point(HN136), and was not given any western medicine about Hypertension during the period of experiment. Group Ⅱ was administrated by moistening Herbal-acupuncture at Blood Pressure Point(HN136), and given western medicine about Hypertension during the period of experiment. we observed the change of systolic and diastolic for 2 weeks, and compared Group Ⅰ with Group Ⅱ . Results: The results obtained as follows: 1. The figure of systolic in Group Ⅰ was decreased, but there was no signification. There was a significant decrease in Group Ⅱ (p<0.05) 2. The figure of diastolic in Group Ⅰ was decreased, but there was no signification. There was a significant decrease in Group Ⅱ (p<0.05) 3. Group Ⅱ was more effective than Group Ⅰ in the results. Conclusion: The results suggest that moistening Herbal-acupuncture at Blood Pressure Point(HN136) was effective treatment of Hypertension. So further research is needed continuously.

입제의 수면처리에 의한 주요수도해충의 동시방제

최승윤,배상희,송유한,Choi Seung Yoon,Bae Sang Hee,Song Yoo Han 한국응용곤충학회 1970 한국식물보호학회지 Vol.9 No.1

본 시험은 유기인계 살충제인 Terracur P입제 및 Lebaycid 입제와 유기염소계 살충제인 상감마입제$(\gamma-BHC 입제)$를 수면시용 하였을때 수도요해충인 이화명충, 끝 동매미충, 애멸구 및 흰둥멸구에 대한 동시방제가능성과 방제적기를 구명하고 약제간의 방제효과를 비교코저 실시하였다. 얻어진 시험결과는 다음과 같다. 1) 약제처리후 부화직후 이화명충 접종 시험에 있어서 상감마와 Terracur P는 높은 살충율을 보였으나 Lebaycid의 살충율은 낮았다. 約 $50\%$ 살충력 잔효일수에 있어서 상감마는 8-14日이었고 Terracur P는 6일간이었다. 2) 당초 계획된 방제적기에 관한 정보는 얻을 수 없었다. 즉 주어진 일간격이 좁아 유의한 차를 얻을 수 없었다. 3) 1,2화기 이화명충에 대한 방제효과는 Terracur P=상감마>Lebaycid의 순이었다. 4) Terracur P와 Lebaycid 입제의 처리는 끝동매미충의 방제효과가 좋았으나 상감마$(\gamma-BHC 입제)$의 방제효과는 기대할 수 없었다. 끝동매미충에 대한 Terracur P와 Lebaycid입제의 잔효력은 30일 이상이었다. 5) 애멸구와 흰등별구에 대한 시험은 충의 서식밀도가 낮기 때문에 약제간 방제효과를 얻을 수 없었다. 6) 공시약제중 Terracur P와 Lebaycidd입제는 이화명충과 끌동매미충의 동시방제제로서 사용할 수 있을 것 같다. This experiment was conducted to find out the simultaneous control effect of granulated insecticides on the several major rice insect pests in Korea. The granulated insecticides used were Terracur P$5\%$ (one of the phosphoric insecticides), Lebaycid $5\%$, and Sang-gamma (gamma-BHC) $6\%$. Those insecticides were broadcasted by hand on the paddy field at the ratio of 3kg. in June and 4kg. in August per 10 a, and the date of insecticidal application was based on the maximum moth occurrence of the rice stem borer in 1969. The rice insect pests tested in this study were rice stem borer (Chilo suppressalis W.), green rice leafhopper (Nephotettix bipunctatus cincticeps U.), smiller brown planthopper (Laodelphax striatellus F.), and white back planthopper (Sogotella furcifera H.). For the study of residual effectiveness of the insecticides, the rice stem borer larvae just hatched from the eggs were installed on the sheath of rice plants collected from the paddy field after insecticidal treatment, and the mortality was made at 48 hours after installation. High mortality was observed in Terracur P and Sang-gamma treatments and low mortality in Lebaycid treatment. The duration of residual effects giving about 50% mortality was about 14 days in Sang-gamma and about 6 days in Terracur P treatments. The study on the effectiveness of the insecticides to the rice stem borer in the paddy field was made by the number of dead blades and white heads of the rice plants injured by the larvae, and the number of survival larvae in the straws after crop harvest. The order of insecticidal effectiveness to the rice stem borer was Terracur P=Sang-gamm>Lebaycid. The study on the effectiveness of the insecticides to the leafhoppers and planthoppers in the paddy field was made by the population density by sweeping (5 times) at the given day intervals after treatments. Terracur P was highly effective for the control of the green rice leafhoppers and Lebaycid was moderately effective, but Sang-gamma seemed not to be effective. The effectiveness of the insecticides tested to the planthoppers was not clear, and it seemed to related with the low insect population density. Study on the determination of proper timing of insecticidal application was not also made clear, and it seemed to be short in the range of day intervals in the insecticidal application. For the study on the control effects of the rice insect pests, rice yield per 100 hills per plot was observed. There was no statistically significant difference among the treatments in rice yields, but the order of yield (Terracur P>Sang-gamma>Lebaycid>Control) was paralleled with the tends of control effects above mentioned. In a view point of simultaneous control of the rice stem borers and green rice leafhoppers by the application of granulated insecticides, Terracur P and Lebaycid were more effective than Sang-gamma which have been used.

서울 북부지역 유통 농산물의 농약 잔류실태

한성희(Sung-Hee Han),박성규(Sung-Kyu Park),김욱희(Ouk-Hee Kim),최영희(Young-Hee Choi),승현정(Hyun-Jung Seoung),이영주(Young-Ju Lee),정희정(Jung-Hee Jung),김윤희(Yun-Hee Kim),유인실(In-Sil Yu),김유경(Yoo-Kyung Kim),한기영(Ki-Young Han 한국농약과학회 2012 농약과학회지 Vol.16 No.2

Phosphorylation of Hepatitis C Virus RNA Polymerases Ser29 and Ser42 by Protein Kinase C-Related Kinase 2 Regulates Viral RNA Replication

Han, Song-Hee,Kim, Seong-Jun,Kim, Eun-Jung,Kim, Tae-Eun,Moon, Jae-Su,Kim, Geon-Woo,Lee, Seung-Hoon,Cho, Kun,Yoo, Jong Shin,Son, Woo Sung,Rhee, Jin-Kyu,Han, Seung Hyun,Oh, Jong-Won American Society for Microbiology 2014 Journal of virology Vol.88 No.19

<P>Hepatitis C virus (HCV) nonstructural protein 5B (NS5B), an RNA-dependent RNA polymerase (RdRp), is the key enzyme for HCV RNA replication. We previously showed that HCV RdRp is phosphorylated by protein kinase C-related kinase 2 (PRK2). In the present study, we used biochemical and reverse-genetics approaches to demonstrate that HCV NS5B phosphorylation is crucial for viral RNA replication in cell culture. Two-dimensional phosphoamino acid analysis revealed that PRK2 phosphorylates NS5B exclusively at its serine residues <I>in vitro</I> and <I>in vivo</I>. Using <I>in vitro</I> kinase assays and mass spectrometry, we identified two phosphorylation sites, Ser29 and Ser42, in the Δ1 finger loop region that interacts with the thumb subdomain of NS5B. Colony-forming assays using drug-selectable HCV subgenomic RNA replicons revealed that preventing phosphorylation by Ala substitution at either Ser29 or Ser42 impairs HCV RNA replication. Furthermore, reverse-genetics studies using HCV infectious clones encoding phosphorylation-defective NS5B confirmed the crucial role of these PRK2 phosphorylation sites in viral RNA replication. Molecular-modeling studies predicted that the phosphorylation of NS5B stabilizes the interactions between its Δ1 loop and thumb subdomain, which are required for the formation of the closed conformation of NS5B known to be important for <I>de novo</I> RNA synthesis. Collectively, our results provide evidence that HCV NS5B phosphorylation has a positive regulatory role in HCV RNA replication.</P><P><B>IMPORTANCE</B> While the role of RNA-dependent RNA polymerases (RdRps) in viral RNA replication is clear, little is known about their functional regulation by phosphorylation. In this study, we addressed several important questions about the function and structure of phosphorylated hepatitis C virus (HCV) nonstructural protein 5B (NS5B). Reverse-genetics studies with HCV replicons encoding phosphorylation-defective NS5B mutants and analysis of their RdRp activities revealed previously unidentified NS5B protein features related to HCV replication and NS5B phosphorylation. These attributes most likely reflect potential structural changes induced by phosphorylation in the Δ1 finger loop region of NS5B with two identified phosphate acceptor sites, Ser29 and Ser42, which may transiently affect the closed conformation of NS5B. Elucidating the effects of dynamic changes in NS5B phosphorylation status during viral replication and their impacts on RNA synthesis will improve our understanding of the molecular mechanisms of NS5B phosphorylation-mediated regulation of HCV replication.</P>

Original Article : Isolation of a Marine Bacterium Capable of Biodegrading Poly(butylene succinate)

( Sang Jun Lee ),( Eun Hee Park ),( Yun Hee Han ),( Young Ok Kim ),( Seong Wook Park ) 한국수산과학회(구 한국수산학회) 2013 Fisheries and Aquatic Sciences Vol.16 No.1

We developed a poly(butylene succinate) (PBS) indicator plate and isolated a marine bacterial colony capable of biodegrading PBS based on the appearance of a clear zone. Growth of the PBS-2 isolate was observed over 4 days of culture at 37°C in PBS-tryptone basal liquid medium, but not in PBS-deprived control medium. The PBS-2 isolate was named Paenibacillus sp. PBS-2 based on 16S rDNA gene sequencing. The PBS-biodegrading marine bacterium isolated in this study will contribute to the effective management of PBS waste problems in marine environments.

A linear programming approach to the benefits of Northeast Asia powergrid interconnections

Hee-In Chang(장희인),Yong Ho Jung(정용호),Yongseok Jung(정용석),Kyu-Won Jeong(정규원),Han-Yeol Choi(최한열),Sang-kwon Kim(김상권) 대한전기학회 2020 대한전기학회 학술대회 논문집 Vol.2020 No.7

Antimicrobial effect of hypochlorous acid on pathogenic microorganisms

( Sang Phil Shin ),( Mun Sup Kim ),( Sung Hee Cho ),( Ji Hyung Kim ),( Casiano H Choresca Jr ),( Jee Eun Han ),( Jin Woo Jun ),( Se Chang Park ) 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1

Disinfection is essential in terms of the public health or environmental hygiene. Hypochlorous acid (HOCI) solution was developed as a disinfectant in Korea. We evaluated the gemicidal activity of HOCI against various pathogenic microbes. Thirty-one ATCC strains were exposed to HOCI solution at various concentrations (20, 40 and 80 ppm) for 1 minute. All the strains of bacteria, yeasts and mycobacteria were killed at 80 ppm after exposure to HOCI. The results suggest that HOCI solution could be used to effectively disinfect public areas.