동종 골수 이식한 백혈병에서 단반복 유전자로 살펴본 혼합 키메라 현상의 의의

장대영,이정신,서철원,이규형,이제환,지현숙,박찬정,한면수,최동원,김정균,최성준,김성배,김상위,김우건,김상희 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1

연구배경 : 동종 골수 이식한 백혈병에서 키메라 현상의 분석은 착상, 질병 재발과 이식 거부를 이해하는데 도움을 줄 수 있다. 최근 STR을 PCR로 증폭한 검사법에 의해 매우 민감하고 유용하게 혼합 키메라 현상을 검출할 수 있다고 보고되고 있으나, 이 방법의 민감도와 임상적 의의는 아직 완전히 정립되지는 않았다. 따라서 PCR-STR검사법이 혼합 키메라 현상을 민감하게 검출하는지 보고, 동종 골수 이식 후 혼합 키메라 현상의 변화 양상을 관찰하고, 키메라 현상의 임상적 의의를 보고자 하였다. 방법 : 형제로부터 동종 골수 이식한 급성 백혈병 6명과 만성 백혈병 2명의 모두 8명을 대상으로 하였다. 44개 골수 검체에서 phenol/chloroform방법에 의하여 단핵세포 DNA를 추출하였다. DNA는 CTT 삼중체, vWA와 amelogenin시발체를 이용하여 증폭하였다. 증폭 산물은 5% 폴리아크랄아미드 젤에서 전기영동 뒤 은 염색으로 확인하였다. 2명의 DNA를 여러 비율로 혼합하여 상기 검사를 하여 검출 가능 최소 농도를 결정하였다. 결과 : 상기 검사법의 검출 가능 최소농도는 0.25%였다. 혼합 키메라 현상은 8명중 4명에서 관찰되었다. 혼합 키메라 현상은 보인 1명에서 급성 및 만성 이식편대 숙주 질환이 나타났고, 완전 공여자 키메라 현상을 보인 1명에서 만성 이식편 대 숙주 질환이 나타났다. 점진적으로 증가하는 혼합 키메라 현상을 보인 2명은 이식 후 12개월에 질병 재발을 보였으나, 완전 공여자 키메라 현상을 보인 경우엔 재발이 관찰되지 않았다. 결론 : PCR-STR검사법은 동종 골수 이식 후 남아 있는 환자 세포를 검출하고 키메라 현상을 관찰하는데 유용한 방법이다. 또한 이 검사법은 동종 골수 이식 후 재발의 조기 진단, 이식편 대 숙주 질환의 평가와 면역 관용에 임상적으로 응용할 수 있으리라 생각한다. Background : Chimerism analysis after allogeneic bone marrow transplantation (BMT) for leukemia could be helpful understanding of the early marrow engraftment, disease relapse, and graft rejection. Recently, a PCR technique which amplifies short tandem repeats(STR) has been reported to be highly sensitive and reliable in detecting mixed chimerism. But its sensitivity and clinical significance has not been established. The purpose of this study was firstly, to confirm whether a PCR-STR in highly sensitive enough ti assess mixed chimerism, secondly to monitor the changing patterns of mixed chimerism after allogeneic BMT, and lastly to determine correlation between the chimeric status and the clinical outcome. Methods : A study was made eight patients (six with acute leukemia and two with chronic leukemia) who underwent unmanipulated allogeneic BMT along with matched donors. DNA was extracted from mononuclear cells of 44 bone marrow samples using a phenol/chloroform method. Amplification of DNA was done using CSFIPO-TPOX-TH01(CTT) triplex with or without vWA or amelogenin primer. The amplified product was separated on 5% polyacrylamide gel and was confirmed by silver staining. The sensitivity of CTT triplex method was determined by mixing DNA from two person in serial proportion using the same method. Results : The sensitivity for CTT triplex method was 0.25% Mixed chimerism was documented in 4 of 8 patients. ONe of four patients with mixed chimerism developed acute and chronic graft versus host disease (GVHD) and one of four patients with complete donor chimerism also developed chronic GVHD. Two patient with progressive mixed chimerism relapsed at 12 months post-BMT, whereas no patient with complete donor chimerism has relapsed. Conclusion : The study has found that PCR-STR was an effective method for the detecting residual host cell and monitoring the chimeric status after allogenic BMT. PCR-STR can also be clinically applicable in early prediction of relapse, appropriate assessment of GVHD, as well as tolerance after allogeneic BMT.

TiO₂를 이용한 아세틸렌 가스의 광촉매 산화반응

정대일,김성엽,이도훈,이용균,박유미,최순규,김동현 동아대학교 환경문제연구소 2000 硏究報告 Vol.23 No.1

Photocatalyzed degradation of acetylene gas was carried out over precoated titanium dioxide(anatase) illuminated UV light in a flow reactor. photocatalytic oxidation of acetylene gas indicated high conversion rates (78.0%) to CO₂gas. And photocatalytic oxidation of gas according to a fold, twofold, and threefold of titanium dioxide(anatase) respectively gave high conversion rates. The longer reaction time was, the higher conversion rate to CO₂ gas showed.

신경병증성 통증 모델쥐 뒤뿌리신경절의 Nitric Oxide Synthase 아형 및 NADPH-diaphorase 발현에 관한 연구

정성균,김수일,양은진,류택근,권오유,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Injury to peripheral nerve somtimes lead to neuropathic pain. Nitric oxide(NO) is increased in the dorsal root ganglia (DRG) of neuropathic pain models, and L-NAME, a nitric oxide synthase (NOS) inhibitor, reduces neuropathic pain. However, it is not known that underlying mechanism of NO involvement in development and maintanence of neuropathic pain. It was found that there were three NOS isoforms-nNOS, eNOS, and iNOS. It is also necessary to investigate NOS isoforms in neuropathic pain. The aim of this study is to investigate NOS isoforms and NADPH-diaphorase expressions and underlying mechanism of NO involvement in neuropathic pain. The 5^th lambar (L5) DRG were obtaind after tight ligation of L5 an L6 spinal nerves for 7 days. Toluidine blue stain, immunohistochemistry for nNOS, eNOS, and iNOS, and NADPH-diaphorase histochemistry were performed. All specimens were observed under light microscope. nNOS expression was increased significantly in the cytoplasm of the DRG of ligated side at 7 day post-ligation (7D PL). eNOS expression was also increased in the blood vessel, a few neurons or satellite cells, and cell membrane of Schwann cells in the DRG of ligated side at 7D PL. iNOS expression was increased in a few blood vessels and cytoplasm of small neurons in the DRG of ligated side at 7D PL. NADPH-diaphorase expression was increased significantly in the neurons of the DRG of ligate side at 7D PL, but NADPH-diaphorase expressions were weak or absent in the blood vessel and Schwann cell in the contralateral DRG and the DRG of ligated side at 7D PL. It is suggested that NOS isoforms may be closely related to development and mantanence of neuropathic pain in order of nNOS>eNOS>iNOS.

金鰲神話 硏究

정상균 서울市立大學校 1992 論文集 Vol.26 No.-

Kim Si-Seup(1435-1493), the first significant writer of imaginative, wrote his immortal Geum-o-sin-hwa influenced by Jeun-deung-sin-hwa. The book enjoyed a great popularity in Korea. But Kim Si-Seup was originally a man who turned from reality, because he could not come to terms with the demand for the renunciation of instinctual satisfaction as it was first made, and who then in fantasy-life allowed fully play to his erotic and ambitious wishes. In Geum-o-sin-wha, Kim Si-Seup's wishful phantasy remains repressed. It is also instructive to consider Geum-o-sin-hwa in the relation of anxiety-dreams. In the dreams, a repressed wish has found a means of evading censorship and the distortion which censorship involves. In Geum-o-sin-hwa, Kim Si-seup's obsessional self-reproaches also appear.

중수지골 관절부위에 발생한 건초 거대 세포종양의 치험례 : A case report

정성균 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Author reports a rare tenosynovial giant cell tumor located adjacent to the third metacarpophalangeal joint of right hand involved bony erosion. Histologically, tenosynivial giant cell tumor is characterized by a number of multinucleated giant cells with a proliferation of synovial cells. This tumor usually occurs on the interphalangeal joint of the hand, especially the terminal interphalgeal joint. It has a benign nature but shows local recurrences. This case is a very rare case occuring in the metacarpophalangeal joint area.

신호분리를 위한 Diplexer에 관한 연구

성정현,김성균,김상혁,김호섭,박준석,안달 호서대학교 반도체제조장비국산화연구센터 2000 학술대회 자료집 Vol.2000 No.1

본 논문에서는 신호의 분리와 합성이 가능한 Diplexer를 제작하였다. 저역 통과 여파기와 고역 통과 여파기의 특성을 정합하여 Diplexer로 구현함으로써 신호의 분리와 합성이 가능하다는 것을 시뮬레이션을 통해 확인하였다. 또한 신호의 분리와 합성의 가능성을 입증하기 위해 실제 마이크로스트립구조의 Diplexer를 제작, 측정한 결과 각각의 통과 대역폭 내에서 0.4dB, 0.8dB이내의 삽입손실과 -20dB의 반사손실이 됨을 실험결과를 통해 알수 있었고 또한, 인덕터의 구현 방법을 마이크로스트립으로 하여 주파수 특성과 재현성을 높였다.

ARQ 기법을 적용한 블루투스 복합 피코넷에서 임펄스 잡음의 영향

김도균,노재성,조성준,김정선 한국항행학회 2002 韓國航行學會論文誌 Vol.6 No.2

본 논문에서는 임펄스 잡음, 라이시안 페이딩, 그리고 동일채널간섭이 존재하는 블루투스 복합 피코넷에서 PER 성능과 패킷 처리율의 개선에 대하여 연구하였다. 특히, 블루투스 복합 피코넷에서 동일채널간섭의 영향을 시뮬레이션 하였다. 시뮬레이션 결과로부터 복합 피코넷에 의한 동일채널간섭과 임펄스 잡음은 블루투스 복합 피코넷의 성능에 영향을 주며 SAW-ARQ 기법은 블루투스 패킷의 전송 성능을 개선하는데 효율적임을 알 수 있었다. This paper has investigated the PER performance and the packet throughput of Bluetooth multipiconet in the presence of impulsive noise. Rician fading, and co-channel interference. Specially, it is simulated the effect of co-channel interference in Bluetooth multi-piconet. From the simulation results, it is obtained that the co-channel interference due to multi-piconet and impulsive noise affects performance of Bluetooth multi-piconet. And, we have known that the SAQ-ARQ method is very efficient to improve the performance of Bluetooth packet transmission

PCB류 노출에 따른 파밤나방 (Spodoptera exigua)의 세대간 발육억제 효과 평가

박상순,정철의,유건상,김용균 7개 국립대학교 환경연구 논문집 공동발행 위원회 2004 공업기술연구 Vol.4 No.-

Polychlorinated biphenyls (PCBs) are persistent, bioaccumulative, toxic and widely distributed environmental chemicals. A previous report indicates that exposure to PCBs causes substantial hazard for survival and development of beet armyworm, Spodoptera exigua, which is a non-target insect species in the environment. This study was focused to evaluate the effect of PCBs on persistent and bioaccumulative toxic action using the susceptible species. For this purpose, PCBs were treated to parent generation and the following progency development was evaluated. PCB-treated female adults showed significant decrease of fecundity (number of oviposition) in dose-dependent manner. The laid eggs differed in successfully hatched larvae, development to pupal stage was also affected by the PCB concentrations exposed during previous parent stage. A life table analysis suggests that the PCB exposure during previous generation can give substantial reduction in population growth of following generation. Thus, the PCB exposure during previous generation would have additive hazard effect on the action of PCBs to S. exigua in the following generation.

혼화재료로서 석분토의 재활용 가능성 평가

이상욱,김성원,오상균,김정길,마상준 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Stone-Dust are inevitable by-products of almost rocky mountains which produce artificial sands in korea. The annual amounts of Stone-Dust is over 80,000㎥ approximately. Almost pre-existing Stone-Dust piled up out in the open space on disposed of industrial waste, it can result in critical environmental problem. The aim of this study is to promote the recycling of industrial waste as a kind of material of admixture. This study conducted some experiments such as pozzolan reaction test and flow test, compressive strength of mortar using Stone-Dust in heat-treatment. As a result of, compressive strength of mortar using stone-dust in heat-treatment is superior in the case of 700℃ 90min.

자궁내막에 발생한 점막 연관 림프조직 (MALT) 림프종 1예

전균호,조현진,박성균,김천복,김대연,김종혁,김용만,김영탁,목정은,남주현 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.4

Non-Hodgkin's 림프종의 약 40%는 결절외(extranodal) 림프종으로 대개 위장관에서 발생하며 이들 중 점막 연관 림프조직(mucosa-associated lymphoid tissue: MALT)에서 기원한 림프종은 MALT 림프종으로 분류된다. 위 외의 부위에서 원발성으로 발생하는 MALT 림프종은 매우 드물지만 실제 거의 모든 신체 장기에 발생하는 것으로 알려져 있는데, 가장 흔한 부위인 위 외에도 폐, 갑상선, 침샘, 눈물샘에 발생하고, 드물게 안구, 유방, 방광, 신장, 흉선 등에도 보고된 바 있다. MALT 림프종은 오랜 기간동안 원격 전이하지 않고 국소 병변으로 남아 있는 특징이 있어 치료 방향 또한 국소 병변의 치료에 집중되며, 예후도 림프절 기원의 림프종에 비해 양호한 것으로 알려져 있다. 본 저자 등은 자궁 내막에 원발성으로 발생한 MALT 림프종 1예를 경험하였기에 문헌 고찰과 함께 이를 보고하고자 한다. Mucosa associated lymphoid tissue (MALT) lymphoma can rarely develop primarily in extranodal sites other than stomach which is the most common site for it. Other rare primary sites are small intestine, colorectum, esophagus, lung, thyroid, salivary gland, lacrymal gland, breast and skin. MALT lymphoma represents a distinct clinicopathologic features: it is usually localized to their original site for a long time and shows much more favorable prognosis than lymphoma at other site, but some MALT lymphoma can arise simultaneously or successively in different organ or give rise to another MALT lymphoma of other organ and can be multifocally disseminated or recurred. We report a very rare case of high grade B-cell lymphoma of mucosa associated lymphoid tissue (MALT) of the uterine endometrium, which was confirmed by immunohistochemical assay.