서울 시내 소아에서 발생한 말라리아 1례

신선희,오필수,김영준,김미란,최하주,윤혜선,박민정,김현태,Shin, Seon Hee,Oh, Phil Soo,Kim, Young Jun,Kim, Mee Ran,Choi, Ha Joo,Yoon, Hae Sun,Park, Min Jung,Kim, Hyun Tae 대한소아감염학회 1997 Pediatric Infection and Vaccine Vol.4 No.2

Malaria due to Plasmodium vivax had been known as an indigenous protozoan disease in Korea. However, massive use of insecticides and improvement of sanitation for several decades have led to rapid reduction of malaria incidence, then it was recognized to have been almost eradicated in recent years. However in 1993, one case was reported in Phajoo, Kyungki-do, and then, reported cases have been increasing annually. Recently we encountered one case of malaria in a 3 year old male child living in Dorim-dong, Seoul, who had never been abroad and had no history of transfusion, drug abuse, and travel to endemic area in Korea. He had characteristic fever, chill and splenomegaly and was confirmed as Plasmodium vivax malaria with peripheral blood smear finding. He was successfully treated with hydroxy chloroquine and primaquine. We report this case with brief review of related literature.

국내 자생 그늘쑥 (Artemisia sylvatica MAX) 모상근의 대량배양을 위한 최적조건

신선희,양덕조,Shin, Sun-Hee,Yang, Deok-Cho 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.1

본 실험은 그늘쑥을 Agrobacterium을 통해 형질전환체인 모상근을 유기하고 모상근의 대량배양을 위해 모상근이 가장 잘 자라는 생장 조건을 찾기 위해 수행되었다. 실험 결과를 종합해 보면 선발된 그늘쑥 모상근 세포주의 대량 생산을 위한 최적 배지는 MS 기본 배지임을 확인하였다. 또한 최적의 havesting time 모상근의 성장률이 좋고 갈변과 배지의 미생물 오염도가 적은 4주가 적당한 것으로 판단된다. 그리고 광 처리 하에서 모상근을 배양하는 것보다는 암상태에서 모상근을 키우고 pH는 5.8 정도가 탄소원은 sucrose로 3.5%가 모상근 을 대량배양시 최적조건임을 확인되었다. 미량원소인 Fe$^{2+}$은 0.1 mM을 처리하는 것이 모상근의 성장을 촉진하였으며 spermine과 spermidine은 각각 10 mM와 100 mM로 처리하는 것이 모상근의 성장을 높일 수 있다. 앞으로 연구는 이 결과를 토대로 모상근 대량 배양을 위한 scale up조건과 bioreator의 설계에 관한 연구가 필요하다. This research aims the production of anti-tumor substances through in vitro culture of hairy roots transformed by Agrobacterium rhizogenes in Artemisia sylvatica MAX and the effect of culture conditions on optimum growth of hairy roots. We investigated the optimum medium, pH, carbon source, sucrose, light, Fe and polyamine conditions of various lines of hairy roots (NK3, NK4, YX. NK3-10) induced from Artemisia sylvatica to increase the optimum growth of hairy roots. MS medium was the best for optimum growth of hairy root clone, NK3-S10. The optimum culture period was 4 weeks for NK3-S10. The optimum sucrose concentration was 3.5%. The optimum concentration of FeSO$_4$, spermine and spermidine was 0.1 mM, 10 mM and 100 mM, respectively.

소방공무원의 외상사건 경험 및 산림산책이 소진에 미치는 영향

신선희,공하성,Shin, Sun-Hee,Kong, Ha-Sung 대한안전경영과학회 2020 대한안전경영과학회지 Vol.22 No.2

The purpose of this study is to explore the effects of fire officers' experience of traumatic events and subsequent forest healing on their occupational burnout. The results are as follows: First, the experience of traumatic events, social and psychological healing, and relieving stress by strolling had a significant effect on burnout. The more fire officers are exposed to traumatic events, the more they lose self-control in social and psychological aspects, and the lower the level of stress relieved through strolling, the higher the level of burnout. Thi sresult suggests that the more fire office rsare called out for fire fighting, the more they see their colleagues with severein juries, and the higher the level of mental and physical an xiety, the more they areex hausted. Moreover, for es thealing help store duce the irmental and physi calfatig uecaused by traumatic events, thu spreven ting them from suffering from burn out. Second, the experience of traumatic events, social and psychological healing, and relieving stress by strolling had a significant impact on occupational stress. The more fire officers are exposed to traumatic events, the more they lose self-control in social and psychological aspects, and the lower the level of stress relieved through strolling, the higher the level of occupational stress. This result indicates that to relieve job stress caused by fire fighting, increased forest healing activities, including various programs such as psychotherapy, developing interpersonal relationships and self-control, and creating a positive mind-set, can reducejobstress and help fire officers recover from it. Lastly, job stress had a mediation effect on the effects of the experience of traumatic events and forest healing on burnout. This implies that burnout can be prevented when fire officers gain high emotional stability through social and psychological healing and stress-relief strolling over a traumatic experience, and stronger support from the organization and family can keep them from being emotionally drained, thus contributing to the prevention of burnout.

백작약 추출물의 Methicillin-Resistant Staphylococcus aureus 에 대한 항균활성

신선희,성인화,Shin, Sun-Hee,Seong, In-Wha 한국미생물학회 2006 미생물학회지 Vol.42 No.1

Dried roots of Paeonia japonica were extracted with dichloromethane, methanol and water aerially. Silica gel column chromatography and thin layer chromatography were used to separate the fractions with antimicrobial activities, and mass spectrometry was used to determine the mass. Dichloromethane extract showed the highest antimicrobial activity. Dichloromethane extract from Paeonia japonica could be a candidate for a new antimicrobial agent against MRSA. 항생제 내성균에 대처 할 수 있는 새로운 항생제의 개발에는 많은 시간과 인력 및 개발비가마 필요하며 새로운 항생제들이 생산되어도 균들은 빠르면 수개월내에 내성을 획득한다. 이 문제의 해결방법의 하나로 전통적으로 감염질환의 치료에 사용되어온 백작약 추출물의 methicillin-resistant Staphylococcus aureus(MRSA)에 대한 항균활성 보유여부를 조사한 결과 백작약의 dichloromethane 추출물이 MRSA에 대하여 높은 항균활성을 나타내어 앞으로 새로운 항균재로서의 개발 및 사용 가능성이 있을 것으로 판단된다.

소아의 아데노바이러스 하기도 감염증: 혈청형 및 임상상

신선희,이환종,윤혜선,Shin, Seon Hee,Lee, Hoan Jong,Yoon, Hae Sun 대한소아감염학회 2002 Pediatric Infection and Vaccine Vol.9 No.2

목 적 : 하기도 감염 환아에서 분리된 아데노바이 러스의 혈청형을 조사하고 유행 양상과 임상상을 분석하고자 하였다. 방 법: 1998년 6월부터 2000년 7월까지 하기도 감염으로 서울대학교 소아과 혹은 인근 지역 종합 병원에 입원한 환아의 비흡인물로 바이러스 분석을 하였다. 검체는HEp-2 세포에서 배양하고 간접 면역 형광 방법으로 아데노바이러스를 검출하였으며, 배양된 바이러스는 항혈청 1, 2, 3, 4, 5, 6, 7, 11을 사용하여 중화 항체법으로 혈청형을 결정하였다. 서울대학교 소아과에 입원한 환아들에서는 후향적으로 의무 기록을 조사하였다. 결 과: 아데노바이러스는 1,305례 중 118례(9.0 %)에서 분리되었으며, 혈청형 별로 3형 46례(39%), 7형 20례(16.9%), 1형은 13례(11.0%), 2형 9례(7.6 %), 4형 9례(7.6%), 6형 7례(5.9%), 11형 3례(2.5%), 5형 1례(0.8%), 비분류 혈청형 10례(8.5%)가 분리되었다. 혈청형 3은 1998년 10월부터 1999년 12월까지 유행하였으며 혈청형 7은 1998년 6월부터 11월까지 유행하였다. 남녀비는1.0 : 0.9이고 평균 연령은 1.95세였다. 임상적인 진단은 폐렴 49례(83%), 급성 기관기관지염 7례(12%), 급성 세기관지염 3례 (5.0%)였다. 관련된 증상과 징후, 검사소견은 기침 (100%), 가래(73.5%), 발열(54.2%), 수포음(59.3%), 천명(34%), 빈혈(35%), 백혈구 감소증(15.8%)였다. 사망률은 전체적으로 13.5%였고, 외래에서 추적관찰을 한 46례 중 15례(32.6%)에서 방사선 소견상 지속적인 이상 소견을 보였다. 결 론 : 아데노바이러스는 소아의 호흡기 감염에서 중요한 역할을 하며 심한 경과를 취하고, 혈청형 3과7의 유행이 있었음을 확인하였다. Purpose : This study was performed to characterize the epidemiologic and clinical features of acute adenoviral lower respiratory tract infections(LRTIs). Methods : Virological analysis was done from respiratory specimens obtained from patients with LRTIs hospitalized to other hospitals and referred to the Department of Pediatrics, Seoul National University Children's Hospital(SNUCH) from June 1998 to July 2000. Viral diagnosis was made by isolation of viruses employing HEp-2 cell culture and indirect immunofluorescent staining with monoclonal antibodies. Serotypes of adenoviruses were determined by neutralization test using antiserum for types 1, 2, 3, 4, 5, 6, 7 and 11. Medical records of children admitted to the SNUCH were reviewed retrospectively. Results : Adenovirus was isolated from 118(9.0%) of 1,305 children with LRTIs. Serotypes were 3(39.0%), 7(16.9%), 1(11.0%), 2(7.6%), 4(7.6%), 6(5.9%), 11(2.5%), and 5(0.8%) and 10 strains(8.5%) were not neutralized by antisera included in the study. Infections by type 3 and type 7 occurred in outbreaks. Male to female ratio was 1.0:0.9 and mean age was 1.95 years. The clinical diagnoses were pneumonia(83%), acute tracheobronchitis(12%) and bronchiolitis(5%). Associated symptoms, signs and abnormal laboratory findings included cough(100%), sputum(73.5%), fever(54.2%), rale(59.3%), wheezing(34%), anemia(35%) and leukopenia(15.8%). Mortality was in 13.5%. Residual radiologic sequelae was identified in 32.6% of the patients followed. Conclusion : These data confirms that adenovirus may cause severe lower respiratory tract diseases, and infections by type 3 and 7 may occured in outbreaks.

이순신 소재 역사소설에 나타난 전통의 전유방식과 타자인식 -신채호와 이광수를 중심으로-

신선희 ( Sun Hee Shin ) 한민족어문학회 2015 韓民族語文學 Vol.0 No.71

This paper is aimed to study how to create the identity of Korean(ChoSeon-In) through biographies at the Early Modern Korea. The texts analyzed are “the greatest naval commander Lee soon-shin (水軍 第一偉人 李舜臣)” serialized in 『Daehan maeil shinbo(Korean Daily News Paper)』 (1908.5.2.~8.18) by Shin Chae-ho and “Lee Soon Shin (李舜臣)” in 『Dong-A Ilbo(East Asia News paper)』(1931.7.16.~1932.4.3) by Lee Kwang-soo. These works deserve attention in terms that they could be regarded as an extension of the intellectual discourses attempting to create the identity of Cho Seon and ChoSeon-in by focusing on Tradition in the patriotic enlightenment era and Japanese colonial era. With the purpose of edifying the modern national characteristics(國性) and patriotism, Shin Chae-ho attempted the modern conversion of Tradition to inbreathe ‘Sate-spirit = National spirit’. On the other hand, Lee Kwang-Soo reflected the 1920s`` cultural nationalism in his work. Eschewing the traditional research approaches classifying and examining the works by the genealogy of Korean literature, this study examined two from the viewpoint of the history of culture. Also this paper focused on how Shin Chae-Ho and Lee Kwang-soo appropriated Tradition in their works, embodied Chinese of Ming Dynasty and Japanese during Japans Invasion of Korea in 1592 and through this, delineated ChoSeon-in.

사회 보호 시설 아동과 일반 가정 아동 간 담화 이해 비교

신선희(Sun Hee Shin),고선희(Sun Hee Ko) 한국언어치료학회 2020 言語治療硏究 Vol.29 No.2

Purpose: Children are exposed to various environments from birth and develop language through interacting with the people around them. However, children in institutional care could experience environmental deprivation compared to children at home because they have been separated from their parents since their childhood and have lived with peers in a limited environment. The purpose of this study was to investigate discourse comprehension, especially inference ability, in children who have different external environments that could affect their language development. Methods: Fifteen children reared at home, 15 children in institutional care, and 15 children with SLI in institutional care participated in the study. They were asked literal and inferential questions after listening to short discourses. To compare the performances of the three groups, a repeated 2-way ANOVA was conducted. Results: The children in institutional care showed significantly better performance compared to the children with SLI in institutional care regardless of the questions’ condition. On the other hand, they performed similar to the children reared at home in the literal questions but significantly poorer than the children reared at home in the inferential questions. Conclusions: These results suggest that the institutional environment that cannot provide individualized experiences or opportunities would influence children’s inference ability.

기능중심의 절차적 교수방법과 완성된 예제 중심의 교수방법이 예비 유아교사의 컴퓨터 리터러시 능력에 미치는 효과 비교

신선희(Shin Sun-Hee),진선미(Jin Sun-Mi),신상희(Shin Sang-Hee) 한국어린이미디어학회 2008 어린이미디어연구 Vol.7 No.1

본 연구의 목적은 예비 유아교사를 대상으로 컴퓨터 리터러시 교육을 실시할 때 기능중심의 절차적 교수방법과 완성된 예제 중심의 교수방법 효과를 비교 검증하는 것이다. 연구대상은 ‘유아컴퓨터’ 교과목 수강생인 H대학의 예비 유아교사 중 실험집단 25명, 통제집단 25명. 총 50명이다. 연구방법은 통제집단의 경우 컴퓨터 활용 관련 기능을 순차적으로 제시하는 기능중심의 절차적 교수방법을 적용하였고, 실험집단에서는 완성된 예제를 제시하고 관련 기능을 학습하도록 하는 교수방법을 활용하여 수업을 진행한 후 학업성취도를 비교하였다. 교육내용은 파워포인트를 활용하여 유아들에게 설명할 수업자료를 개발하는 것과 동영상을 편집하는 것이다. 연구결과는 완성된 예제 중심의 교수방법을 적용한 집단에서 사용의 용이성, 유아의 적합성, 교육적 가치, 흥미성, 디자인에 관한 컴퓨터 리터러시 능력이 높게 나왔다. The purpose of the study is to investigate and compare quantitatively two different approaches of the instructional methods comprising of the procedural instructions or the worked-out examples. The investigation was performed on the computer literacy training courses in the H university of which total 50 preschool preparatory teachers taking a children computer class were participating. These participants were divided into two groups of equal number; experimental group and control group. To these two groups, a procedural instruction composed of sequential study of individual computer functions was applied to the control group, while an worked-out example based instruction was applied to the experimental group. Contents of the instructions applied were mainly composed of the preparation of the teaching materials for preschool children including a text type presentation and a multimedia movie. The learning achievement of participants was estimated in terms of 5 parameters; the usability, the suitability for the preschool children, the educational value, the degree of amusement, and the excellency of visual design. The obtained results were analyzed by quantitative statistical means and the learning achievements of each groups were compared based on this analysis. The results of the analysis suggests that the experimental group to which an worked-out example was applied exhibits higher level of computer literacy in every parameters analyzed in the study.

유아기 자녀를 둔 어머니의 놀이성과 유아의 정서능력 및 사회적 능력 간의 관계

신선희(Sun Hee Shin),우수경(Soo Kyeong Woo) 한국생활과학회 2012 한국생활과학회 학술대회 Vol.2012 No.11

중규모 기상모형에서 지표면 경사를 고려한 직달 복사량의 효과

신선희 ( Sun Hee Shin ),이영선 ( Young Sun Lee ),하경자 ( Kyung Ja Ha ) 한국지리정보학회 2006 한국지리정보학회지 Vol.9 No.4

In this study, the effects of the surface topographical characteristics on the meteorological fields are examined in a mesoscale meteorlolgical model. We calculated the direct solar radiation using the illumination angle considering the inclination of topography and tried to find out its effect on meteorological fields. In above experiments, we selected two cases for the clear day and the cloudy day to show the effect of weather and represented the results for two cases. In the correction of the direct solar radiation, the results of two cases indicate that there are obvious differences on the steep Taeback and Soback mountains. And on the time-series analysis the east-facing slope of these mountains receives the more direct solar radiation about 10-60W/㎡ in the morning hours but lesser in the afternoon hours than the horizontal surface while it is opposite on the west-facing slope. And the results mentioned above are more obvious at clear day. With the same analysis method, at clear day, the surface skin temperature is higher at all hours than that on horizontal surface on the both of slope. At cloudy and rainy day, the surface skin temperature on the east-facing slope is higher in the morning hours but lower in the afternoon hours than that on horizontal surface. But on the west-facing slope, it is higher at all hours than that on horizontal surface. In the two cases, the temperature considering the slope of surface is almost higher than that on the horizontal surface. The wind is stronger than that on the horizontal surface with increasing pressure gradient force according as increasing temperature gradient around the Taeback and the Soback mountains.