Cycloheximide가 膵臟腺房細胞에 미치는 影響에 關한 微細形態學的 硏究

徐仁壽,洪錫宰 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

蛋白合成障碍가 膵臟腺房細胞의 微細構造 및 自家貪食力에 미치는 影響을 알아보기 爲해 흰쥐에 cycloheximide와 自家貪食亢進劑인 neutral red를 單獨 또 倂合 投與하여 惹起된 微細形態學的 變化를 電子顯微鏡으로 觀察하였던 바를 要約하면 다음과 같다. Cycloheximide는 膵臟腺房細胞에 比較的 初期에서부터 Golgi complex의 腫脹, ER膜에 附着된 ribosome의 脫落을 主로 한 輕한 變化를 일으켰다. 60分이 經過하면 ER 및 mitochondria의 輕한 腫脹, 脫落된 ribosome의 破壞 및 消失等을 볼 수 있었으나 그 程度는 輕微하였다. Autophagic vacuole의 計數는 對照群이 한 腺房當 10.1±2.2個, neutral red投與群이 54.4±4.9個, cycloheximide와 neutral red를 同時에 投與한 群에서는 21.5±4.1개, cycloheximide投與 30分 後 neutral red 投與群에서는 9.7±2.7個이었다. 이와 같은 事實은 cycloheximide가 autophagic vacuole形成에 抑制的 效果가 있다는 것을 意味하며 autophagic vacuole의 形成에는 어떤 形態로든 새로운 蛋白合成이 必要하다는 것을 示唆하는 것이라 생각된다. This study was carried out to investigate the effects of cycloheximide, known as an inhibitor of protein synthesis at the level of polypetide elongation, on the fine structures of pancreatic acinar cells. Also studied was the effects of protein synthesis inhibition by cycloheximide on the neutral red-induced autophagocytosis. Twenty-one male albino rats, maintained on water only for 12 hours, weighing 200㎎. in average, were divided into 5 groups, 3 rats for each groups except Group 2. Group 1: This is to serve as a control group. Two consecutive doses of 0.4㎖. of saline per 100 gm. of body weight were given intraperitoneally to 3 rats at interval of 30 minutes. Group 2: A dose of 2 mg. of cycloheximide per 100gm. of body weight was given to 9 rats intraperitoneally and 3 animals each were sacrificed 30, 60 and 90 minutes after injection, respectively. Group 3: A dose of 2mg. of neutral red per 100gm. of body weight was given to 3 rats intraperitoneally, 30 minutes after intraperitoneal injection of 0.4 ㎖ of saline per gm. of body weight. Group 4: A dose of 2㎎. of neutral red per 100gm. of body weight was given to 3 rats intraperitoneally, 30 minutes after intraperitioneal injection of 2㎎. of cycloheximide per 100gm. of body weight. Group 5: Simultaneous intraperitoneal injection of 2 ㎎. of neutral red and 2㎎. of cycloheximide per 100gm. of body weight was given to 3 rats. All animals were sacrificed 60 minutes after the the last injection except Group 2. Pancreas extirpated for both light and electron microscopic studies. The results were summarized as follow: Cycloheximide induced moderate dilatation of Golgi complex aad detachment of ribosomes from RER membrane from early stage (sacrificed 30 minute after injection). Mild swelling of RER and mitochondria along with destruction and disappearance of detached ribosomes were noted in the animals sacrificed 60 and 90 minutes after injection (Group 2). The number of autophagic vacules per acinus was 10.1±2.2 in the control group (Group 1); 54.4±4.9 in neutral red only group (Group 3); 21.5±4.1 in neutral red simultaneous with cycloheximide group(Group 5); 9.7±2.7 in the group with neutural red administered 30 minutes after cycloheximide injection(Group 4). The results showed marked inhibitory effects of cycloheximide on the neutral red-induced autophagocytosis, suggesting need for do novo synthesis of protein in the formation of the autophagic vacuoles.

環境汚染賠償責任保險制度의 導入에 관한 考察

徐圭錫,李守培 全北大學校 1996 論文集 Vol.42 No.-

This paper is a study on the introduction of Environmental Impairment Liability Insurance System. Nowadays, environmental pollution is one of the most serious problems with which modern societies are confronted. It belongs not only to one country but all countries. Therefore the environmental problem han become an international problem, and has risen to the surface as one of the biggest pending problems between the advanced and the developing countries. The peril that proceeds from the pollution of environment is different from the usual one, it procceds gradually and keeps going on for a long period of time. It is hard to predict the environmental pollution. And once pollution occurs, the damage is generally on a grand scale. Until now, the relief by judicial procedure and administrational procedure, and private relief is used for the method of the relief from the damage caused by environmental pollution. But in order to help the victim promptly and protect the enterprise of the invasion from the damage of the environmental pollution, it needs to establish the system of the Environmental Impairment Liability Insurance. This paper tries to aim at the study on the elements of Environmental Impairment Liability Insurance and seeking for introducing method of it to our country. the content of this paper follows; Chapter Ⅰ. Introduction Chapter Ⅱ. The conception of Environmental Impairment Liability Insurance; meaning, type, function, feature. Chapter Ⅲ. The elements of Environmental Impairment Liability Insurance System; the insured, the accident of Insurance, the scope of indemnity, the escape clause, the period of insurance, premium rate, joint insurance, etc. Chapter Ⅳ. A Study on the Introduction of Environmental Impairment Liability Insurance System in advanced countries such as Germany, Sweden, America and Japan. Chapter Ⅴ. Conclusion

고관절 전 치환 성형술 후 탈구에 관한 치험 및 연구

서유성,김동진,박종석,임수재,나수균,최창욱 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Dislocation is one of the serious complications in total hip replacement arthroplasties. The purpose of this study is to evaluate the risk factors of dislocation, including age, sex, preoperative diagnosis and bilaterality, history of previous hip surgery, positioning of component, soft tissue tension, treatment after dislocation or redislocation. Authors reviewed 190 patients of total hip replacement arthroplasty, which were performed from Jan. 1990 to Dec. 1996. 15 cases developed dislocation after surgery. Incidence of dislocation was 7.89%(15 of 190 cases). Dislocation after revision was not happened(0 of 18 cases). Inclination of acetabular cup was average 37.5° (range: 31° - 43° ). Average anteversion of the acetabular component was 18.1" by Woo and Morrey method, 11.4° by Hassan method. They were normal range except 3 cases. Dislocation was not prevented even though inclination and anteversion of acetabular cup were within normal range. Soft tissue tension is determined by change of limb and femoral neck length. Average limb length difference was -0.2mm(range; -l0mm - +10mm) and average offset difference was +1.7mm(range;-4mm - +18mm). Revision must be accomplished as soon as possible when limb shortening happen by upward migration of the acetabular component. In conclusion, anteversion of acetabular cup may be most important factor in our cases, so cup position must be fixed in proper position during operation.

제2종 중도절단에 근거한 지수분포의 신뢰함수 추정

강석복,서영수,이광호 嶺南大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.17 No.-

In this paper, we shall propose several reliability estimators in the two-parameter exponential distribution, and compare the proposed estimators in terms of the MSE through the Monte Carlo simulation.

관동맥질환에서 각종 지질 및 지단백의 변화

황석순,김권배,손수인,서영숙,김기식,김윤년 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.3

To evaluate the relationship between coronary artery disease(CAD) and the various levels of lipid and lipoproteins, we performed coronary angiography to the patient, who were suspected coronary artery disease from January 1988 to August 1989. And compared it with normal control group. The number of control group were 41(21 men, 20 women), and the patient group were 52(42 men, 10 women). The age was not differ between two groups. The results were as follows; 1. In CAD group, the plasma concentration of total lipid, triglyceride, total cholesterol, phosphlipid, and TC/HDL ratio were significantly higher than in control group. But, though, the concentration of HDL was lower in CAD group, had no significant difference between two groups. 2. The severity of CAD were well correlated with total lipid, cholesterol, LDL, trigylceride, phospholipid, TC/HDL, LDL/HDL levels. HDL level didnt's show significant correlation with the degree of CAD. We concluded that total lipid, cholesterol, trigylceride TC/LDL ratio were valuable markers of CAD in Korean, but futhur studies of lipoproteiins and other risk factors in the large population will be recommended.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

Alloy 600 합금의 내부식성 향상을 위한 레이저 표면 합금화

김정수,서정훈,강석중,신진국,국일현 한국레이저가공학회 1999 한국레이저가공학회지 Vol.2 No.1

The surface of Alloy 600 was alloyed using a continuous wave CO₂ laser beam in order to improve its corrosion resistance. Laser surface alloying (LSA) was done by melting the surface electroplated with Cr of the alloy. The Cr concentration of the alloyed surface was 28∼30 at.%, which is similar to that of Alloy 690. Alloying elements in the alloyed layer was observed to be distributed very homogeneously all over the alloyed region. According to the electrochemical and modified Huey tests, the corrosion resistance, in particular the grain boundary corrosion resistance, of the LSA specimens was significantly improved, compared with that of the as-received(AR) specimen. This improved corrosion resistance of the alloyed specimen might be attributed to the high Cr content, which could make possible formation of more stable and dense passive film onto its surface.

전압의존성 칼슘채널 α1A 및 α1E subunit 유전자의 사람 염색체 상의 위치 및 흰쥐신경계에서의 발현양상 규명

김현,박수연,서영석,유임주,김형래,박선화 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.2

韓國産 草烏屬 植物의 生藥學的 硏究 (第2報) : 싹눈바꽃 및 개싹눈바꽃에 관하여 On the Aconitum proliferum NAKAI and Aconitum pseudoproliferum NAKAI

朴鍾喜,金泰洙,徐錫洙 부산대학교 신약개발연구소 1988 藥學硏究誌 Vol.22 No.1

미생물 초기부착과 생물막 성장에 미치는 기질의 C/N 비

박영식,이석렬,나영수,서정호,송승구 부산대학교 환경문제연구소 1997 環境硏究報 Vol.15 No.1