Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122의 생산과 그 특성

최용석,옥승호,유주현,배동현 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.1

토양으로부터 분리한 Metarrhizium anisopliae (Metschn.) Sorok이 생산하는 biopolymer를 정제하여 분석하고, 그의 생산조건을 검토하였다. 탄소원으로 mannitol, 질소원으로는 polypepton을 사용했을 때 가장 많은 biopolymer를 생산하였고 K_2HPO_4 및 CaCl_2를 첨가함으로서 생산량이 더욱 증가하였다. 또한 이 biopolymer를 정제하여 그 분자량과 구성성분을 검토한 결과 Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122는 분자량이 1.7×10 exp (6)이고, C, H의 구성비가 1:2이며 미량의 N이 검출되었으나, 주 구성성분은 glucose와 galactose의 환원당으로 이루어져있는 전혀 새로운 biopolymer인 것으로 나타났다. To produce biopolymer, Metarrhizium anisopliae (Metschn.) Sorok was cultured in a medium containing glucose 1.0%, sucrose 2.0%, soluble starch 1.0%, yeast extract 0.5%, polypeptone 0.5%, K_2HPO_4 0.1%, MgSO_4·7H_2O 0.02%. The culture broth was centrifuged and the polymer was harvested by adding methanol to the culture supernatant. When three times of methanol was added, the polymer was coagulated and precipitated. Then it was further purifued through successive SK-1B, SA-20P, HP-20 column chromatographies. This polymer was designated as Biopolymer YU-122. C:H ratio of this Biopolymer YU-122 was 1:2 and small amount of N is detected by CHN analyzer. Glucose and galactose are main components of this polymer. Average molecular weight of this biopolymer was 1.7×10 exp (6) by Sepharose 4B gel permeation chromatography. Optimal condition for biopolymer production was investigated. When 5% of mannitol was used as a carbon source, and polypepton as a N source, highest productivity of biopolymer was achieved. C/N ratio as nutrient was also a major factor in polymer production and its optimal ratio was 3.

대학 영어독해 지도를 위한 제안

유관현,강현숙 서울産業大學校 2002 논문집 Vol.53 No.1

Currently, most English reading classes in Korea are still pursuing the long tradition of grammar translation method, not only in the instruction itself, but also in tests following the instruction. However, intensive teaching of grammar and translation hasn't brought much successful results in enhancing effective reading. The purpose of this paper is to integrate the new trend of language teaching paradigm into classrooms. This paper will briefly review, first, the notion of reading and its processes including schema theory. Secondly, factors that need to be considered when planning reading class are examined. They are characteristics of receptive language skills, critical literacy, student autonomy, alternative assessment, culture education, vocabulary instruction and reflective teaching. Thirdly, how the considerations can be applied in classrooms is discussed. Many possible suggestions are included. Some suggestions are from the real classroom applications which have been tried already in the present writers' classrooms. When the mentioned theories are applied into reading instructions, it is possible that reading class will have more variety and yield more effective reading.

아므르불가사리 (Asterias amurensis)의 배우자를 이용한 남해해역 연안해수의 생물학적 수질평가

유춘만,박지인,조기안,주현수,박종천,나명석 한국환경과학회 1999 한국환경과학회지 Vol.8 No.1

This study was carried out to evaluate off-shore water quality and to draw marine pollution map to Southern sea in Korea by bioassay using gametes and early development system of Asterias amurensis. From the bioassay, it was determined that the water qualites of Southern sea maintained the grade Ⅱ, because development of A. amurensis were moderately inhibited. However, Sunchon, Kwangyang, and Jinhae Bay belonged to grade Ⅲ, because development of A. amurensis were strongly inhibited. This could be due to the inflows of several river systems and poor water circulation. The water quality at Yosu island was the grade I, because A. amurensis were not inhibited in its early development.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

가족성 아밀로이드성 말초신경병증 1예

양우석,서민정,권유석,유현정,노숙영,백소아 대한근전도전기진단의학회 2003 대한근전도 전기진단의학회지 Vol.5 No.2

Metarrhizium anisopliae (Metschn.) Sorok이 생산하는 Biopolymer YU-122의 물리, 화학적 특성

최용석(Yong-Suk, Choi),옥승호(Seung-Ho Ohk),유주현(Ju-Hyun Yu),배동훈(Dong-Hoon Bai) 한국식품과학회 1997 한국식품과학회지 Vol.29 No.1

다중회귀모형을 이용한 수완부 골성숙도의 추정에 관한 연구

김경호,유형석,김석현 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.5

성장의 잠재력에 대한 평가는 개체의 성숙도를 나타내는 여러 생리학적 지표(physiologic indicators)들에 의해 이루어지며 그 중 골성숙도(skeletal maturity)는 성적성숙도(sexual maturity)와 신장의 성장변화와 밀접한 관계가 있는 것으로 알려져 있으나, 치아발육과의 상관관계에 대해선 논란이 많은 실정이다. 그러나 최근의 연구에 의하면 하악견치를 포함한 일부치아의 발육이 골성숙도와 밀접한 관련이 있는 것으로 알려져 있으나, 치아발육과의 상관관계에 대해선 논란이 많은 실정이다. 그러나 최근의 연구에 의하면 하악 견치를 포함한 일부 치아의 발육이 골성숙도와 밀접한 관련이 있는 것으로 보고된 바 있다. 이에, 본 연구에서는 7세에서 15세까지의 한국인 남, 녀 아동 387명의 수완부 방사선 사진과 파노라마 사진을 이용하여 Fishman방법과 Greulich와 Pyle방법에 의해 골성숙도을 평가하였으며 변형된 Demirjiran방법에 의해 치아의 성숙도를 평가하여, 수완부 방사선의 사진의 도움 없이 골성숙도를 추정할수 있는 방법을 구하고자 다중회귀모형을 이용한 수완부 골성숙도의 추정에 관한 연구를 시행하였으며 다음과 같은 결론을 얻었다. 1. 골성숙 지수의 추정을 위한 아래의 다중회귀모형은 84%의 설명력을 나타내며 연대연령, 성별, 하악 견치의 회귀계수는 통계학적 유의성을 보였다. 골성숙지수 = 0.60x 연대연령 -1.67x성별 + 0.88x하악견치 - 0.05x하악 제2대구치 -10.3 2. 골연령의 추정을 위한 아래의 다중회귀모형은 87%의 설명력을 나타내며 연대연령, 성별, 하악 견치의 회귀계수는 통계학적 유의성을 보였다. 골연령 = 0.75x 연대연령 - 0.55x성별 + 0.71x하악 견치 + 0.09x하악 제2대구치- 5.77 (성별: 남자=1, 여자=0., 하악견치, 제2대구치 : 각 발육단계별 평균연령) The evaluation of growth potency can be done with many physiologic indicators. It has been well known that skeletal maturity has a close relation with both sexual maturity somatic maturity, but the correlation between skeletal maturity and dental maturity was believed to be less certain. But, recent studies show that specific teeth, including lower canines, present close correlations with skeletal maturity. So, in this study, we study, we studied hand-wrist X-ray films and orthopantomograms of 387 Korean boys and girls aged from 7 to 15; the purpose was to determine skeletal and dental maturity, and to find out a new method to estimate individual skeletal maturity using multiple-regression model, without the help of hand-wrist X-ray film. As a result of this study, followings were observed. 1. The following multiple-regression model can estimate skeletal maturity index(SMI) with 84% of accuracy, and regression coefficient of chronologic age, sex and lower canine show statistical significance. SMI = 0.60 x chronologic age - 1.67 x sex** + 0.88 x lower canine* - 0.05 x lower 2nd molar* - 10.3 *: mean age corresponding each developing atage, **: male= 1, femal = 0 2. The following multiple-regression model can estimate skeletal age with 87% of accuracy, and regression coefficient of chronologic age, sex and lower canine show statistical significance. Skeletal age = 0.75 x chronologic age - 0.55 x sex** + 0.71 x lower canine* - 0.09 x lower 2nd molar* - 5.77 *: mean age corresponding each developing atage, **: male= 1, femal = 0

TiO₂를 이용한 아세틸렌, 프로필렌, 프로판 가스의 광촉매 산화반응

정대일,변석인,이도훈,이용균,박유미,최순규,김동현 동아대학교 환경문제연구소 2002 硏究報告 Vol.25 No.1

Photocatalyzed degradation of acetylene, propylene, and propane gases were carried out over precoated titanium dioxide(anatase) illuminated UV light in a flow reactor. photocatalytic oxidation of acetylene, propylene, and propane gases respectively indicated high conversion rates (78.0%) to CO₂gas. And photocatalytic oxidation of three gases according to a fold, twofold, and threefold of titanium dioxide(anatase) respectively gave high conversion rate. The longer reaction time was, the higher conversion rate to CO₂gas showed.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

Synthesis of Quinoline derivatives with Various ketones

Suk-Hyun Joung,Ju-Hyun Song,Eu-Gene Kim,Hee-Jin Seo,Yu-Mi Park,Dai-Il Jung,Jung-Tai Hahn 한국생명과학회 2004 한국생명과학회 심포지움 Vol.43 No.-