急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철산,강원권,김민범,김삼용,노홍규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1991 No.-

急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

To evaluate the in vitro granulocyte-macrophage colony formation in acute myfloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2 × 10 exp (5) cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defi ned as containg 20 or more cells, and cluster was defined as containing 3-19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2 × 10 exp (5) cells (20±19)and that of colonies was 5-24/2 × 10 exp (5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2 × 10 exp (5) cells(18±16) and that of colonies was 6-13/2 × 10 exp (5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation: 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases Were `non-forming', 2 cases were `cluster forming'; and 3cases wer cluster and `colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of `cluster forming'; and none of 3 cases of `cluster and colony forming' achieved complete remission. So, there was significa nt difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

急性骨髓性 白血症에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1 No.1

To evaluate the in vitro granulocyte-macrophage colony formation in acute myeloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2×105 cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defined as containg 20 or more cells, and cluster was defined as containing 3 - 19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2×10^(5) cells (20±19)and that of colonies was 5-24/2×10^(5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2×10^(5) cells(18±16) and that of colonies was 6-13/2×10^(5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation : 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases were 'non-forming', 2 cases were 'cluster forming'; and 3cases wer cluster and 'colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of 'cluster forming'; and none of 3 cases of 'cluster and colony forming' achieved complete remission. So, there was significant difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

콩나물蛋白質의 營養學的 硏究

梁且範,文南順,尹錫權 漢陽大學校 師範大學 1981 論文集 Vol.- No.1

Nutritional evalution of proteins from soybean and soybean sprout and effect of methionine supplement to soybean sprout was studied by feeding albino rat. Sprague-Dawley strain of albino rats fed for 15 days with 5% (Group 1) and 10% (Group 2) level of protein from soybean, 10%(Group 3), 15% (Group 4) and 20%(Group 5) level of protein from soybean sprout, methionine supplement to soybean sprout (Group 6) and 10% level of protein form casein as a reference (Group 7). The results are as follows 1. Body weight gain was greater in soybean than soybean sprout in same protein level. Methionine supplementation increased weight gain significantly compared to soybean sprout alone. 2. By increasing protein level of soybean sprout, weight gain was increased significantly. 3. The more rat gained weight, the more rat in take food and there was significant correlationship between weight gain and food intake. 4. Protein intake was related to weight gain and there was significant positive correlationship between them. 5. PER was increased from 1.061 to 1.962 by adding methionine to soybean sprout, and PER increment was low by increasing the level of protein from soybean sprout in diet. There are no correlationship between PER and weight gain. 6. By adding methionine to soybean sprout, chemical score was increased. Chemical score showed significant positive correlationship with PER but not with weight gain. EAAI and RI was positively correlated significantly with PER.

생쥐 안구에서 산화적 손상에 의한 superoxide dismutases와 metallothionein의 나이에 따른 유도의 차이

이홍영,이태범,안춘산,변익건,최석민,권대승,최철희 朝鮮大學校 附設 醫學硏究所 2003 The Medical Journal of Chosun University Vol.28 No.2

The basal levels and inducibility of superoxide dismutases (SODs; Mn-SOD and Cu/Zn-SOD) and metallothionein (MT) was investigated in the eyes of mice with age. Oxidative stress was induced by paraquat, an intracellular superoxide generator, in 1, 4, 8, and 12 months of age and then mRl\As of SODs and MT in the eyes were determined by RT-PCR assay. The basal level of Mn-SOD mRNA increased from 1 month to 8 months but decreased thereafter. Mn-SOD mRNA was induced by paraquat until 4 month but not after 8 months. On the other hand, the basal level of Cu/Zn-SOD mRNA decreased with age. Cu/Zn-SOD mRNA was not induced by paraquat until 4 months. The basal level of MT mRNA declined with age whereas its inducibility by paraquat was intact at all ages. Taken together, the results indicate that SODs and MT are differentially expressed and induced according to the age. It could be also implicated that failure in induction of Mn-SOD with age may be one of causative factors in the aging process whereas induction of Cu/Zn-SOD and MT may be one of important factors in defense against oxidative stress in the eyes. 서론: 최근 노화는 반응성 산소종에 의한 산화적 손상의 축적결과라는 설이 각광을 받고 있다. 노화과정에서 노화와 관련된 질병을 비교적 잘 나타내는 안구를 선정하고, 정상생쥐의 안구에서의 superoxide dismutases (SODs) 와 metallothionein (MT) 의 함량 뿐 아니라 산화성 스트레스를 유발하는 paraquat에 의한 SODs와 MT의 유도능과 나이와의 상호관계를 조사하여 이들의 노화과정에서의 역할을 규명하고자 하였다. 재료 및 방법 : 본 연구에서 1, 4, 8, 12개월된 정상생쥐의 안구에서 반응성 산소종 생성의 초기에 중요한 방어기전인 SODs mRNA와 hydroxy radical (OH·)에 의한 산화적 손상의 마지막 방어기전인 MT mRNA의 함량과 산화성 스트레스를 유발하는 paraquat에 의한 유도능을 역전사 중합효소 연쇄반응 (RT-PCR) 으로 조사하였다. 결과: 정상생쥐의 안구의 노화과정에서 Mn-SOD와 Cu/Zn-SOD, 그리고 MT mRNA의 항정상태 함량을 조사한 결과, Mn-SOD mRNA는 8개월까지는 증가하다가 12개월에서 감소하였으나, Cu/Zn-SOD mRNA는 나이와 함께 감소하였다. 그러나 MT mRNA 는 8개월까지는 감소하다가 12개월에서 약간 증가하였다. 산화성 스트레스를 유발하는 parraquat에 의하여 Mn-SOD mRNA는 1개월과 4개월에서만 유도되었고, Cu/Zn-SOD mRNA는 8개월과 12개월에서만 유도되었으며, MT mRNA는 관찰한 전 기간에서 유도되었다. 결론: 이상의 결과를 종합하면 SODs와 MT는 안구에서 나이에 따라 서로 다르게 발현되고 유도되는 것으로 생각되며, 노화과정에서 Mn-SOD유도의 이상이 노화의 원인이 되는 인자로 작용하고, 반면에 Cu/Zn-SOD와 MT의 정상적인 유도능이 산화적 스트레스에 대하여 방어역할을 하는 것으로 생각된다. 이와 같이 연령이 증가함에 따라 Mn-SOD, Cu-Zn-SOD, 및 MT의 발현과 유도능의 차이가 있게 되고, 그 결과 반응성 산소종에 의한 산화적 손상의 정도의 차이로 노화의 속도가 조절되지 않나 생각된다.

Thermus caldophius GK24로부터 DNA Iigase 유전자의 클로닝 및 발현

김현규,이정범,정승은,권석태 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.2

Thermostable DNA ligase is an essential enzyme for the ligase chain reaction(LCR), a method of detection of a single point mutation. Firstly reported thermostable DNA ligase gene was Tth lig gene of Thermus thermophilus HB8 in 1991. We amplified a new thermostable DNA ligase gene of Thermous caldophilus GK24 using the method of PCR. Used degenerate primers DLI and DLT were designed based on Tth DNA ligase gene. This amplified Tca DNA ligase gene was cloned and expressed. For DNA subcloning and sequencing, restriction enzyme sites of the gene were analyzed.

Studying removal behavior of single-crystal sapphire during orthogonal ultra-precision machining based on slip/fracture activation model

Suk Bum Kwon,Sangkee Min 한국생산제조학회 2022 한국생산제조시스템학회 학술발표대회 논문집 Vol.2022 No.5

Studying Crack Generation Mechanism in Single-Crystal Sapphire During Ultra-precision Machining by MD Simulation-Based Slip/Fracture Activation Model

Suk Bum Kwon,Aditya Nagaraj,Dalei Xi,Yiyang Du,Dae Nyoung Kim,Woo Kyun Kim,Sangkee Min 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.5

Single-crystal sapphire is used in various fields of industry due to its superior mechanical, optical, and chemical properties, but fabricating sapphire is challenging because of its high hardness and brittleness. Ultra-precision machining (UPM) has been studied as one of the solutions to overcome these issues as it facilitates cutting of brittle ceramics in a ductile manner. However, the material removal mechanisms during UPM of single-crystal sapphire is not yet fully understood. This paper studied the crack initiation mechanism in single-crystal sapphire during UPM with relation to the crystallographic properties of sapphire depending on the cutting direction. By projecting a given stress distribution from molecular dynamics simulation on to different slip and fracture planes, the likelihood of plastic deformation and cleavage fracture was calculated, and respective crack initiation mechanisms were identified. The proposed MD simulation model and analysis were verified through ultra-precision orthogonal plunge cut experiments along the same crystallographic directions as the simulations.

Molecular layer-by-layer assembled forward osmosis membranes

Kwon, Soon-Bum,Lee, Jong Suk,Kwon, Soon Jin,Yun, Seong-Taek,Lee, Seockheon,Lee, Jung-Hyun Elsevier 2015 Journal of membrane science Vol.488 No.-

<P><B>Abstract</B></P> <P>A recently devised, molecular layer-by-layer (mLbL) approach based on the alternative cross-linking of monomers was employed to fabricate high-performance thin film composite (TFC) forward osmosis (FO) membranes with excellent rejection toward monovalent NaCl salt. An ultrathin and highly dense polyamide (PA) selective layer with a precisely controlled structure was created on a tailored porous support via mLbL. The intrinsic separation properties of the mLbL-assembled TFC membranes were tuned by adjusting the mLbL cycle number to optimize the FO performance. The best FO performance was achieved at 10 mLbL cycles (mLbL-10), where the permeability and selectivity were properly balanced. Importantly, the mLbL-10 membrane exhibited superior FO performance compared to the commercial HTI FO membranes as well as hand-cast TFC membranes prepared by the conventional interfacial polymerization: the mLbL-10 membrane showed ~3.5 times higher water flux, ~60% lower reverse salt flux and ~85% lower specific salt flux compared to the cellulose triacetate HTI membrane, with 0.5M NaCl draw solution and DI water feed solution in FO mode. The stability and the associated membrane performance of the mLbL-assembled membrane depending on the ionic strength of the environment were explained by the swelling behavior of the polyelectrolyte-assembled interlayer adhered underneath the PA selective layer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PA TFC FO membrane is fabricated via a molecular layer-by-layer (mLbL). </LI> <LI> Separation properties are tuned by adjusting the mLbL cycle number. </LI> <LI> The mLbL achieves higher membrane permselectivity than interfacial polymerization. </LI> <LI> The mLbL-assembled membrane shows FO performance exceeding the commercial one. </LI> <LI> The mLbL-assembled membrane׳ stability at high ionic strengths should be improved. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

영산강 수계에서 과불화 화합물의 모니터링

권범근(Bum Gun Kwon),임채승(Chae Sung Lim),임혜정(Hye Jung Lim),나숙현(Suk Hyun Na),권중근(Joongkeun Kwon),정선용(Seon Yong Chung) 大韓環境工學會 2015 대한환경공학회지 Vol.37 No.5

이 연구는 영산강 수계 중 수체를 대상으로 10종의 과불화 화합물 (perfluorinated compounds, PFCs)로 인한 오염실태를 파악하기 위한 모니터링을 수행 하였다. 시료채취는 영산강 전수계에 걸쳐 있는 총 18지점에서 이루어졌고, 5-6월인 봄철과 10월인 가을철 두 번에 걸쳐 시료가 채취되었다. 10종의 PFCs 오염물을 대상물질로 하여 분석한 결과, 물 시료 중 농도는 PFOS (perfluorooctanesulfonate)가 20.8-92.0 ng/L, PFOA (perfluorooctanoate)가 method detection limit (MDL)-28.40 ng/L로 검출되었으며 PFNA (Perfluorononanoate)가 MDL-42.20 ng/L, PFHxS (perfluorohexanesulfonate)가 MDL-11.47 ng/L로 나타났다. PFOS는 봄과 가을철 모두에서 검출빈도와 그 농도가 높았고, PFOA와 PFNA는 대체로 봄철이, PFHxS의 경우는 가을철에 검출빈도와 농도가 높은 것으로 조사되었다. 하지만 상기 4종을 제외한 다른 PFCs 화학종은 검출빈도가 낮고 매우 낮은 농도인 것으로 나타났다. 하·폐수처리장이 위치한 광주의 도심지역 수계지역의 수체 시료에서 검출된 PFCs의 농도는 타 영산강수계내 수체 시료에 비해 상대적으로 높은 것으로 나타났다. To determine the concentrations of selected 10 perfluorinated compounds (PFCs), a field study was conducted in the water body of Yeongsan River Water System. Raw water samples were collected in the spring and the fall, respectively, which included 18 sampling sites. Collected samples were equally mixed and then served as an analytical sample. The concentration of perfluorooctanesulfonate (PFOS) and perfluorooctanoate (PFOA) were in range of 20.80-92.0 ng/L and ND-28.40 ng/L respectively. Perfluorononanoate (PFNA) and perfluorohexanesulfonate (PFHxS) were ranged from ND to 42.20 ng/L and from ND to 11.47 ng/L. The detection frequencies of other PFCs selected in this study were very sparse at very low concentrations, except for PFOS, PFOA, PFNA and PFHxS. PFOS was higher detection frequency and concentration in both spring and fall, PFOA and PFNA were in spring, and PFHxS was in fall. As a result, the observed concentrations of PFCs in the downtown water area of Gwangju, located in the wastewater treatment plants, were relatively higher than other sampling points.