간호실무에서 신봉이론(Espoused theories)에 대한 탐색적 연구

서문자,김혜숙,이은희,박영숙,조경숙,강현숙,임난영,김주현,이소우,조복희,이명하,지성애,하양숙,손영희,권성복,김희진,추진아 성인간호학회 2001 성인간호학회지 Vol.13 No.1

As a nursing practice involves nurses' actions in a specific context of health care, this study has focused on exploring the espoused theories in nursing practice within the action science perspectives, Espoused theories are the belief, principles, and rationale expressed by the practitioner as guiding her/his actions in a situation of practice. The data were analysed qualitatively and 25 elements of espoused theories of nursing action were identified and clustered into 6 categories. The 25 elements of espoused theories are as follows: The clinical nurse worked in wholistic and individual nursing, focussed on the patient's needed, comfort and supportive nursing (5 theories of nursing goal) ; excellent skills, knowledge based, assessment and data collection, explaining, educating or a scientific basis(6 theories of nursing intervention): advocacy, value oriented, treatment, account- ability and commitment(4 theories of nursing ethics) ; human respect. partnership, trust(3 theories of patient-nurse relationship) : knowledgable, accumulated clinical experiences and personally lived experiences. positive perspectives(4 theories of nurse), role of intervention. rewarding peer relationship(3 theories of situations) The above mentioned espoused theories are similar to that of nursing textbooks which students learned through basic nursing education and almost the same as the Acts ofa Nurse in Korean. However, we are doubtful whether nurses actually do as they think. Therefore. it is recommended to review the theories-in-use in order to and any discrepancies between the espoused theories and the reality of nursing actions

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

健康한 韓國人의 動脈血液 가스 및 酸一鹽基 平衡値에 對한 觀察

崔震守,曺方煥,朴喜淑,金顯承,金瑛,白正敏,徐錫助 순천향의과대학 1979 Journal of Soonchunhyang Medical Science Vol.2 No.3

The purpose of this paper is to make an attempt in this investigation to determine normal adult values of arterial blood gas and acid-base balance in 53 healthy Korean male and female subjects of varying age and then compare with the data obtained by others. Determinations of the acid-base status of the arterial blood were performed at 37℃ by the Astrup method using a radiometer instruments, consisted of BGA2A/3-MK 2 system. The mean and standard errors of each component were as follows: pH 7.42±0.043, oxygen saturation 96.96±0.089%, pO?? 97.66±0.91mmHg, pCO?? 37.08±0.68mmHg, CO?? content 24.29±0.43mM/L, bicarbonate 23.26±0.68mM/L, buffer base 46.56±0.66mEg/L, and base excess -0.024±0.001mEg/S. Compared with normal adult values in literatures, all the components of the data appear to be in the same range without any significant difference.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

사망진단서(사체검안서) 상의 선행사인으로부터 사망통계의 원사인이 선정되는 비율 : 3개 대학병원에서 교부된 사망진단서를 중심으로

박우성,박석건,정철원,김우철,탁우택,김부연,서순원,김광환,서진숙,부유경 한국의료QA학회 2004 한국의료질향상학회지 Vol.11 No.1

Background : To exatnine the problems intolved in writing practice of death certificates, we cotnpated the determination of underlying cause of death for wital statistics using recorded underlying cause of death in issued death statistics. Methods : We collected 688 rnortality certificates issue in year of 2,000 from 3 university hospitals. And we also collected vital statistics from ministry of statistics. The causes of death were coded by experienced medical record wpecialists. And causes of death determined at ministry of statistics for national vita statistics were mapped to causes of death recorded at each death certificates. The rate that underlying causes of death for vital statistics were derived from underlying causes of death recorded at issued death certificaties sere analysed. Results : 64.5% of underlying cause of death for could be derived from underlying cause of death recorded at issued death certificates, 8.6% derived from intermediate cause of death, and 3.9% derived from direct cause of death. In 23% of cases, underlying cause of death could not be derived using issued death certificates. The rate that underlying cause of death for vital statistics could be derived from underlying cause of death recorded at death certificates was different between 3 university hospitals. Ane the rate was also different between death certificates and postmortem certificates. We classified the causes of death using 21 major categories. The rate was different between diseases or conditions tha caused death too. Conclusion : When we examined the correctness of death certificate writing practice using abpve methods, cortectness of writing could not be told as satisfactory. There was difference in correctness of writing between hospotals, between death certificates and postmortem certificates, and between diseases and conditions that caused death. With this results, we suggested some strategy to improve the correctness of death certificate writing practice.

Intracellular delivery of cell-penetrating peptide-transcriptional factor fusion protein and its role in selective osteogenesis

Suh, Jin Sook,Lee, Jue Yeon,Choi, Yoon Jung,You, Hyung Keun,Hong, Seong-Doo,Chung, Chong Pyoung,Park, Yoon Jeong Dove Medical Press 2014 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.9 No.-

<P>Protein-transduction technology has been attempted to deliver macromolecular materials, including protein, nucleic acids, and polymeric drugs, for either diagnosis or therapeutic purposes. Herein, fusion protein composed of an arginine-rich cell-penetrating peptide, termed low-molecular-weight protamine (LMWP), and a transcriptional coactivator with a PDZ-binding motif (TAZ) protein was prepared and applied in combination with biomaterials to increase bone-forming capacity. TAZ has been recently identified as a specific osteogenic stimulating transcriptional coactivator in human mesenchymal stem cell (hMSC) differentiation, while simultaneously blocking adipogenic differentiation. However, TAZ by itself cannot penetrate the cells, and thus needs a transfection tool for translocalization. The LMWP-TAZ fusion proteins were efficiently translocalized into the cytosol of hMSCs. The hMSCs treated with cell-penetrating LMWP-TAZ exhibited increased expression of osteoblastic genes and protein, producing significantly higher quantities of mineralized matrix compared to free TAZ. In contrast, adipogenic differentiation of the hMSCs was blocked by treatment of LMWP-TAZ fusion protein, as reflected by reduced marker-protein expression, adipocyte fatty acid-binding protein 2, and peroxisome proliferator-activated receptor-γ messenger ribonucleic acid levels. LMWP-TAZ was applied in alginate gel for the purpose of localization and controlled release. The LMWP-TAZ fusion protein-loaded alginate gel matrix significantly increased bone formation in rabbit calvarial defects compared with alginate gel matrix mixed with free TAZ protein. The protein transduction of TAZ fused with cell-penetrating LMWP peptide was able selectively to stimulate osteogenesis in vitro and in vivo. Taken together, this fusion protein-transduction technology for osteogenic protein can thus be applied in combination with biomaterials for tissue regeneration and controlled release for tissue-engineering purposes.</P>

Control of cancer stem cell like population by intracellular target identification followed by the treatment with peptide-siRNA complex

Suh, Jin Sook,Lee, Hyun Jung,Nam, Hyun,Jo, Beom Soo,Lee, Dong Woo,Kim, Ji-Hye,Lee, Jue Yeon,Chung, Chong Pyoung,Lee, Gene,Park, Yoon Jeong Academic Press 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>Cancer stem cells (CSCs) are a subpopulation of cancer cells and have been known to create cancer reoccurrence during cancer therapy due to their stem cell-like characteristics. However, exact target to control the CSC has not been fully established. Here, we enriched CD44<SUP>High</SUP> population of MDA-MB-231 cells by CD44 antibody as a CSC marker. By Phospho Antibody Array, CD44<SUP>High</SUP> population of MDA-MB-231 cells reveals Feline sarcoma-related tyrosine kinase (FER) protein was highly activated. When FER siRNA and low molecular weight protamine (LMWP) as cell penetrating peptides are applied to this population, cancer migration and colony forming ability are inhibited. Moreover, silencing FER using FER siRNA and LMWP conjugates enhances anti-metastasis related factors including E-cadherin, p75 and p63. Taken together, FER is a new marker for targeting breast CSCs and peptide-mediated siRNA method could be an effective and safe way of delivery and be a new therapeutic strategy for targeting breast cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> FER protein is highly activated in the cancer stem cells derived from human breast tumors. </LI> <LI> Silencing FER by siRNA reduces stemness and metastatic features of cancer stem cells. </LI> <LI> FER siRNA complex with the cell-penetrating peptide showed more effective and less toxic method. </LI> </UL> </P>

예비유아교사의 아동권리 감수성 증진을 위한 문제중심학습(Problem-based Learning)의 효과

서혜정(Suh, Hye-Jeong),김진숙(Kim, Jin-Sook),서영숙(Suh, Young-Sook) 한국유아교육·보육복지학회 2010 유아교육·보육복지연구 Vol.14 No.1

본 연구에서는 H대학 예비유아교사 2학년 51명을 대상으로 6주간 문제중심학습(Problem-Based Learning)을 실시한 후 아동권리감수성 증진 효과를 검증하고자 하였다. 동일한 집단을 대상으로 프로그램을 처치하기 전과 프로그램을 처치한 후 문제중심학습으로 실시한 프로그램의 효과를 paried t-검증을 통해 사전 사후 검사의 차이를 분석하였다. 그 결과 하루일과계획, 게임활동, 자리 및 순서 정하기, 요리활동, 자유선택활동, 견학계획에서 아동권리감수성에 대한 사전사후 검사의 차이가 유의하게 효과적인 것으로 나타났다. 예비유아교사들은 ‘유아권리’ 프리즘으로 유아교실을 들여다 본 경험을 통해 유아권리 존중이란 유아에게 주도권 주기와 유아의 의견 경청에서부터 시작되는 것이라 생각하게 되었다. ‘유아권리’라는 프리즘은 예비유아교사들에게 유아권리 존중의 파수꾼 역할을 하게 되었다. 따라서 본 연구는 예비유아교사의 아동권리감수성 향상을 위해 문제중심학습 효과를 드러낸 것으로 유아교육기관에 종사하게 될 예비유아교사들을 위해 다양한 상황마다 아동권리감수성 향상을 위한 프로그램 개발의 필요성이 있음을 제안하였다. 또한 유아교사양성교육에서 기존의 교과목과 권리감수성을 연결하는 것이 중요함을 시사하고 있다. In this study, 51 pre-service early childhood teachers in their second year at J College were gathered for 6 weeks to see if there was more sensitivity of children’s rights after applying the problem-based learning method. Using the paired t-test for sensitivity, the difference between pre-test and post-test was analyzed. In the study, the following activities were observed: starting the day with telling students the agenda, game activity, giving assigned seating, cooking activity, free-time, and field trips. It was found that there was a .001 difference in the preliminary teacher’s value of children’s rights in the post test. With the experience of participating in the supervising of infants prism, those preparing to become preschool instructors found that respect starts from instilling leadership skills in the infants and also listening attentively to the infants’ opinions. The prism for the supervising of infants allowed those in preparation to become preschool instructors to take on a role to look out for the children in the respect of supervising the children. According to this study, the problem-based learning method had an effect on the pre-service early childhood teacher’s sensitivity of children’s rights. therefore, it was found that there is a need to develop a program for the promotion of children’s rights in certain situations. In addition, it has been concluded that it is important to make this method a basic subject in adolescent education and an effective way to create sensitivity.

유아권리와 권리교육에 대한 보육교사의 인식 및 요구 분석

서영숙(Suh, Young-Sook),서혜정(Suh, Hye-Jeong),김진숙(Kim, Jin-Sook) 한국유아교육·보육복지학회 2009 유아교육·보육복지연구 Vol.13 No.3

이 연구는 보육 현장에서 유아권리 인식을 고취시키고 이를 실현시킬 수 있는 일차적 책임을 지닌 보육교사들의 유아권리 및 유아권리교육의 필요성에 대한 인식과 유아권리교육에 대한 요구를 조사하기 위해 보육교사 916명을 대상으로 연구자들이 작성한 질문지 조사를 실시하였다. 연구 결과, 첫째, 보육교사들의 유아권리 인식은 교사변인에 따라 차이가 있어 4년제 대졸 교사로서 국공립이나 법인보육시설에 근무하는 경력이 많은 보육교사의 유아권리인식이 가장 높았다. 그러나 유아권리교육 필요성 인식은 교사변인에 따른 차이는 없었다. 둘째, 보육교사의 유아권리인식이 높을수록 유아권리교육 필요성 인식이 높았다. 셋째, 보육교사는 보육현장에서 유아권리교육이 제대로 실시되지 않는 이유로 자료 및 프로그램 부족, 교육방법론 부족으로 보았으며, 지침서와 프로그램, 그리고 다양한 교재 교구를 요구하였다. 넷째, 보육교사는 유아권리교육 연수 참여 의지가 높았으며, 국가기관과 대학이 연수의 주최가 되길 요구하였다. 연구결과를 중심으로 유아권리교육 증진 방안에 대한 제안이 이루어졌다. This study investigated child care-giving teachers’ recognition of children’s rights and the necessity of teacher education on respecting children’s rights in early child care and education centers. Questionnaires of 916 teachers working at early child care and education centers were analyzed. Following results were obtained: First, teachers of public funded centers having 4-years university graduation degree and more teaching experiences were showed to have higher recognition of children’s rights, however, there were no differences in teachers’s recognition of the necessity of teacher education on children’s right care and education according to teachers’ variables. Second, teachers’ recognition of children’s rights appeared to have positive relationship with their recognition of the necessity of teacher education on respecting children’s right in early childhood settings. Third, teachers requested various teaching materials, such as teacher guide book, child activity program for better children’s rights respecting care and education.

Poster Session 1 : Analytical biochemistry Biochemical pharmacology and natural product chemistry Biotechnology ; 1-Palmitoyl-2-Linoleoyl-3-acetyl-glycerol isolated from deer antler, cervus nippon and its synthetic enantiomer potentiate the mitogenic acti

( Jeong Sook Suh ),( Soo Young Ko ),( Yun Jin Lee ),( Jin Kwon ),( So Yeop Han ),( Yoon Soo Bae ),( Jae Soon Eun ),( Jin Kyung Lhim ),( Gil Ja Jhon ) 한국생화학분자생물학회 (구 한국생화학회) 2000 추계학술대회집 Vol.2000 No.-