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RISS 인기검색어
- 검색키워드
- 저자 : Subhas C. Kundu (검색결과 5 건)
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Biomimetic Designing of Functional Silk Nanotopography Using Self-assembly
Kundu, Banani,Eltohamy, Mohamed,Yadavalli, Vamsi K.,Kundu, Subhas C.,Kim, Hae-Won American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.42
<P>In nature inorganic organic building units create multifunctional hierarchical architectures. Organic silk protein is particularly attractive in this respect because of its micro-nanoscale structural blocks that are attributed to sophisticated hierarchical assembly imparting flexibility and compressibility to designed biohybrid materials. In the present study, aqueous silk fibroin is assembled to form nano/microtopography on inorganic silica surface via a facile diffusion-limited aggregation process. This process is driven by electrostatic interaction and only possible at a specified aminated surface chemistry. The self-assembled topography depends on the age and concentration of protein solution as well as on the surface charge distribution of the template. The self-assembled silk trails closely resemble natural cypress leaf architecture, which is considered a structural analogue of neuronal cortex. This assembled surface significantly enhances anchorage of neuronal cell and cytoskeletal extensions, providing an effective nano/microtopographical cue for cellular recognition and guidance.</P>
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Molecular Phylogeny of Silk Producing Insects Based on Internal Transcribed Spacer DNA1
Mahendran, Botlagunta,Ghosh, Sudip K.,Kundu, Subhas C. Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.5
Silk moths are the best studied silk secreting insects and belong to the families Bombycidae and Saturniidae. The phylogenetic relationship between eleven silk producing insects was analyzed using the complete DNA sequence of the internal transcribed spacer DNA 1 locus. The PCR amplification and sequence analysis showed variation in length ranging from 138 bp (Antheraea polyphemus) to 911 bp (Hyalopora cecropia). Microsatellite sequences were found and was be used to distinguish Saturniidae and Bombycidae members. The nucleotide sequences were aligned manually and used for construction of phylogenetic trees based on Maximum parsimony and Maximum likelihood methods. The topology in both the approaches yielded a similar tree that supports the ancestral position of the Antheraea assama.
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박아름,김성곤,박영환,김현정,김민근,권해용,Subhas C. Kundu 한국고분자학회 2015 Macromolecular Research Vol.23 No.10
A silk fibroin (SF) and poly-ε-caprolactone (PCL) tri-layered nano-fibrous scaffold composed of an inner, middle, and outer layer is fabricated via sequential electrospinning. The middle layer of the SF/PCL blend nano-fiber is introduced to minimize delamination of each layer of the scaffold. The inner layer is composed of SF with Spirulina maxima extract (SP), and the outer is composed of PCL. The anti-thrombic effects of SP are tested first. The structure, mechanical properties, and cytocompatibility of the scaffold are evaluated. The tri-layered nano-fiber scaffold is implanted into a rat carotid artery, and the sample after 3 weeks of implantation is evaluated histologically. The SP exerts anti-thrombic activity, and the SF with SP inhibits platelet adhesion. The tri-layered scaffold with the middle layer composed of SF/PCL blend exhibits excellent tensile strengths, burst pressure strength, and suture retention strength. The fabricated material does not induce any cytotoxicity. The cells are well spread on the scaffold. Recipient vessel maintains patency 3 weeks after implantation. The inner lumen of the scaffold reveals regenerated endothelial cells. The results indicate that the tri-layered tubular SF/PCL vascular grafts can be used in vascular tissue engineering due to their excellent mechanical properties and good tissue regeneration capability.
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Bhattacharjee, Promita,Naskar, Deboki,Kim, Hae-Won,Maiti, Tapas K.,Bhattacharya, Debasis,Kundu, Subhas C. Elsevier 2015 European polymer journal Vol.71 No.-
<P><B>Abstract</B></P> <P>A comparative study of nanofibrous scaffolds with inclusion of nonmulberry silk protein fibroin is presented for application in bone tissue engineering. Introduction of silk fibroin into the scaffolds is carried out in two ways: by electrospinning blend of poly(ε-caprolactone) (PCL) and by grafting fibroin on aminolyzed electrospun nanofibrous PCL. Verification of aminolysis was provided by confocal laser microscopy of rhodamine B isothiocyanate tagged substrates. Absorbance spectroscopy of the products of the reaction between NH<SUB>2</SUB> groups and ninhydrin was used for quantification of aminolysis. Presence of nitrogen on the substrates was established using energy dispersive X-ray while scanning electron microscopy was used to substantiate their nanofibrous morphology. Evaluation of ATR-FTIR results showed that secondary structure of fibroin was preserved in the respective substrates. Presence of fibroin improves hydrophilicity, measured by dynamic contact angle, and surface roughness, topography viewed by atomic force microscopy. These characteristics support cell growth and proliferation. The mechanical strength of the scaffolds is enhanced due to presence of fibroin. Different biophysical characterizations indicate better hydrophilicity, higher nitrogen content, and higher surface roughness of the fibroin grafted scaffolds. Both fibroin-grafted and fibroin-blended scaffolds successfully support activity and viability of human osteoblast like cells. Cell cycle analysis, alkaline phosphatase assay and Alizarin red S staining are used to substantiate cell cycle pattern, proliferation and resultant neo-matrix generation on the scaffolds respectively. The results show that fibroin grafted matrices are better at supporting cell adhesion, growth, and proliferation. The findings demonstrate advantages of fibroin blended and grafted matrices for use in bone tissue engineering applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Silk fibroin from <I>Antheraea mylitta</I> was included in nanofibrous PCL matrices. </LI> <LI> Inclusion was carried out either by grafting or by blending the fibroin. </LI> <LI> Inclusion of fibroin improved strength and biocompatibility of the matrices. </LI> <LI> Fibroin grafted matrices were comparatively more biocompatible. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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