http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Su Ryun Choi,Sang Heon Oh,Vignesh Dhandapani,Chang Soon Jang,Chun-Hee Ahn,Jana Jeevan Rameneni,Hyuna Kim,Inbae Jeon,Yong Pyo Lim 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.2
Traditional breeding methods usually involve fi eld tests carried out by experienced breeders. However, such methods arecostly and time-consuming. Recently, with the development of Next-Generation Sequencing (NGS) technology, molecularmarkers are being utilized for selection processes in breeding. To implement a high-throughput system using molecularmarkers in Chinese cabbage ( Brassica rapa subsp. pekinensis ) breeding, we developed single nucleotide polymorphism(SNP) marker sets for background selection and testing F 1 purity using Fluidigm genotyping assays. SNPs were generatedusing NGS technology on 209 varieties of Chinese cabbage collected from around the world. Those with minor allele frequency≥ 5% and polymorphism information content ≥ 0.3 were screened, and then based on the physical distribution amongthe 10 chromosomes, 177 SNPs were selected and synthesized for testing. To obtain marker sets with high selection effi ciency,we tested 192 SNPs on 45 types of inbred lines and 29 types of F 1 hybrids. Among the 192 SNPs, we selected 96 markerssets for background selection and 24 marker sets for F 1 purity testing according to the following criteria; the genotype of theparents was homozygous, and the F 1 follows the parents’ genotypes. These SNP sets are suitable for high-throughput systemsusing the 96.96 and 192.24 integrated fl uidic circuit platforms of Fluidigm genotyping assays. These SNP marker sets are notonly effi cient for selecting of early fi xed lines as background selection but are also useful for testing the purity of F 1 hybrids.
Su Ryun Choi,Sang Heon Oh,Parameswari Paul,Sushil Satish Chhapekar,Jana Jeevan Rameneni,Vignesh Dhandapani,Song Yeon Han,Chang Yeol Lee,Gyung Ja Choi,Yong Pyo Lim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Clubroot (CR) caused by Plasmodiophora brassicae is a severe disease that decreases crop quality and productivity in cruciferous crops. Clubroot resistance linked quantitative trait loci and candidate genes have been identified during last two decades. However, disease lead crop damage continues to occur owing to differences in host variety and constant pathogen variation. Therefore, continued development of markers is required, and the underlying regulatory mechanisms such as the interrelationships between genes and the way in which genes are regulated need to be investigated. MicroRNAs (miRNAs) are attracting attention as regulators of gene expression, including during biotic stress responses. We try to understand how miRNAs regulate clubroot resistance-related genes in P. brassicae-infected Brassica rapa. Two Brassica miRNAs, Bra-miR1885a and Bra-miR1885b, were revealed to target TIR-NBS genes. In non-infected plants, both miRNAs were expressed at low levels to maintain the balance between plant development and basal immunity. However, their expression levels increased in P. brassicae-infected plants. Both miRNAs down regulated the expression of the TIR-NBS genes Bra019412 and Bra019410, which are located at a clubroot resistance-related quantitative trait locus. A qRT-PCR analysis revealed Bra019412 expression was negatively regulated by miR1885. A 5-prime rapid amplification of cDNA ends analysis confirmed the cleavage of Bra019412 by Bra-miR1885b. Thus, miR1885s potentially regulate CR resistance associated TIR-NBS gene expression during P. brassicae infections of B. rapa. Additionally, we have identified the candidate genes against ‘Banglim’ pathotype by fine-mapping with two inbred line 09CR500 and 09CR501. And we developed the novel gene-based markers based on ORF1, ORF2, ORF3 encoding TNL. And using diverse public available information, we have tested the marker availability with core collection population and improved. Those marker information will be useful in Brassica breeding programs such as marker-assisted selection and gene pyramiding to identify and develop resistant line and cultivars.
QTL mapping of Fusarium wilt resistance in radish (Raphanus sativus L.)
Xiaona Yu,Su Ryun Choi,Yong Pyo Lim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Fusarium wilt (FW), caused by the soil-borne fungal pathogen Fusarium oxysporum is a serious disease in cruciferous plants, including the radish (Raphanus sativus). To identify quantitative trait loci (QTL) or gene(s) conferring resistance to FW, we constructed a genetic map of R. sativus using an F2 mapping population derived by crossing the inbred lines ‘835’ (susceptible) and ‘B2’ (resistant). A total of 220 markers distributed in 9 linkage groups (LGs) were mapped in the Raphanus genome, covering a distance of 1041.5 cM with an average distance between adjacent markers of 4.7 cM. Comparative analysis of the R. sativus genome with that of Arabidopsis thaliana and Brassica rapa revealed 21 and 22 conserved syntenic regions, respectively. QTL mapping detected a total of 8 loci conferring FW resistance that were distributed on 4 LGs, namely, 2, 3, 6, and 7 of the Raphanus genome. Of the detected QTL, 3 QTLs (2 on LG 3 and 1 on LG 7) were constitutively detected throughout the 2-years experiment. QTL analysis of LG 3, flanked by ACMP0609 and cnu_mBRPGM0085, showed a comparatively higher logarithm of the odds (LOD) value and percentage of phenotypic variation. Synteny analysis using the linked markers to this QTL showed homology to A. thaliana chromosome 3, which contains disease-resistance gene clusters, suggesting conservation of resistance genes between them.
Development Of SNP Markers And Genome-wide Association Study In Chinese Cabbage
Wenxing Pang,Nirala Ramchiary,Su Ryun Choi,Yong Pyo Lim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Genome-wide association study (GWAS) is a very powerful method to identify the natural allelic variation present in crop plants causing variation to economically important traits. The recent advances in high throughput genotyping and sequencing technology supplemented greatly to GWAS. Taking this advantage, we selected a total of 382 Chinese cabbage inbred lines for GWAS study. The selected inbred lines are being sequenced using next generation sequencing technology to develop genome wide gene specific single nucleotide polymorphism markers. The morphological and quality traits data were taken from field grown inbred lines. The phenotype and genotype association study will be done with more environmental grown data’s and developed SNP. At the end of this project, gene specific SNP markers will be developed for Chinese cabbage breeding for morphological and quality traits.