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The structural basis of eukaryotic chaperonin TRiC/CCT: Action and folding
Hyunmin Kim,Junsun Park,Soung-Hun Roh Korean Society for Molecular and Cellular Biology 2024 Molecules and cells Vol.47 No.3
Accurate folding of proteins in living cells often requires the cooperative support of molecular chaperones. Eukaryotic group II chaperonin Tailless complex polypeptide 1-Ring Complex (TRiC) accomplishes this task by providing a folding chamber for the substrate that is regulated by an Adenosine triphosphate (ATP) hydrolysis-dependent cycle. Once delivered to and recognized by TRiC, the nascent substrate enters the folding chamber and undergoes folding and release in a stepwise manner. During the process, TRiC subunits and cochaperones such as prefoldin and phosducin-like proteins interact with the substrate to assist the overall folding process in a substrate-specific manner. Coevolution between the components is supposed to consult the binding specificity and ultimately expand the substrate repertoire assisted by the chaperone network. This review describes the TRiC chaperonin and the substrate folding process guided by the TRiC network in cooperation with cochaperones, specifically focusing on recent progress in structural analyses.
Grid selection strategy for high-resolution cryo-EM
Junsun Park,Ji-yeon Park,Seowon Lee,Soung-Hun Roh 한국구조생물학회 2020 Biodesign Vol.8 No.2
Cryo-electron microscopy (cryo-EM) is a revolutionary technique to study the three-dimensional structure of macromolecules and theirs complexes at molecular resolution. The first step in preparing samples for cryo-EM is to select and optimize the right grid for the specimen. This screening process needs consideration in many aspects including concentration and stability of the specimen, compatibility with grid material and optimum ice thickness across the grid. Importantly, the best signal-to-noise ratio (SNR) for a micrograph is closely related to vitrifying the grid sample with the optimum imaging condition. Here we describe overall strategies for grid selection and optimization by understanding the properties of grids and a variety of techniques for grid treatment for high-resolution electron micrographs. This review also describes the utilization of various specimen supports including amorphous carbon, graphene and functionalized support films.
수상레저스포츠 참가 대학생의 라이프스타일이 자아존중감 및 건강증진행위에 미치는 영향
윤형기(Yoon Hyung-Gi),노재헌(Roh Jae-Hun),주성택(Ju Soung-Teg) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.2
The principal purpose of this study is to analyze closely the causal relationship among lifestyle, self-seteem and health promotion behaveiors of the universities participating in sports for all of the workers. For this purpose the research was administered to using materials of the 313 universities participating in water leisure sports of Seoul and kyunggi-do. Data were collected through samples by means of purposive sampling method. The principal purpose of this study is to analyze closely the causal relationship among lifestyle, self-esteem, and health promotion behaviors of the Universities participating in water leisure sports. The questionnaire for data collection of this study was based on those used in previous studies among lifestyle, self-esteem and health promotion behaviros scales, and used them after examing the verification of validity and reliability through pilot test. The methods of Statistics used to analyze the collected datas were corelation and multiple regression analysis. The following is conlusions brought through these analyzing methods and process in this study. Hopothesis Ⅰ was selected to optimal casual relationship. Hopothesis Ⅱ was selected to optimal casual relationship. Hopothesis Ⅲ was selected to optimal casual relationship.
Integrated cryoEM imaging center at Seoul National University, Korea
Miji Jeon,Jun Bae Park,Hansol Lee,Gyuhee Kim,Eung Baek Kim,Mingyu Jung,Soung-Hun Roh 한국구조생물학회 2021 Biodesign Vol.9 No.4
The Center for Macromolecular and Cell Imaging (CMCI) is a core facility equipped with multiple cryo-capable electron microscopes including a 200 kV Thermofisher Glacios, 200 kV JEOL JEM2100Plus and 120 kV FEI TECNAI with direct electron detector technologies. The center also has auxiliary equipment for optimal specimen preparation, such as for protein purification, tissue culture, grid preparation and vitrification. The center offers to the scientific community the best practice of cutting-edge cryogenic electron microscopy (cryoEM) technology for specimen optimization, high-resolution data collection and structure determination. The center also develops the strategy of an integrated cryoEM imaging platform to visualize molecular architectures from purified proteins to cells.