In vitro Mammalian Chromosomal Aberration Test of Fullerene-C60

Soo Jin Kim(김수진),Kyung Taek Rim(임경택),Hae Won Cho(조해원),Jeong Hee Han(한정희),Hyeon Yeong Kim(김현영),Jeong Sun Yang(양정선) 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.1

Fullerene의 유전독성을 평가하기 위하여 Chinese hamster유래의 난소유아세포(CHO-K1 cell)를 이용하여 직접법(-S9)과 대사활성화법(+S9 mix)의 염색체이상시험을 실시하였다. 시험물질은 1% CMC 나트륨염의 현탁액(l% CMC 용액)에 희석하여 조제하였다. 대사활성화를 시키지 않은 직접법의 염색체이상시험에서 24시간 투여군은 8단계의 농도(0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)로 투여하여 실시하였다. 투여 농도 증가에 따른 염색체이상의 빈도가 증가하는 양상이 나타나지 않았다. 48시간의 투여군에서는 8단계의 농도(0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)로 투여하여 실시하였는데 투여 농도 증가에 따른 염색체이상의 빈도가 증가하는 양상이 나타나지 않았다. 배수체의 염색체이상은 직접법에서 관찰되지 않았다. 대사활성화법을 이용하여 6시간 시험물질을 투여한 시험에 있어서는 8단계의 용량단계 (0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)를 설정하였는데 투여 농도가 증가함에 따른 염색체이상빈도의 증가양상이 관찰되지 않았다. 이상의 결과를 종합할 때 본 시험물질은 본 시험 조건하에서 CHO-K1세포에서 대사활성화를 시켰을 때 염색체 이상을 유발하지 않는 것으로 판단된다.

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Renca 마우스 종양모델에서 지구성운동이 종양형성에 미치는 영향

조정제,안현종,김성수,김창주 대한스포츠의학회 2003 대한스포츠의학회지 Vol.21 No.1

Subcutaneous injection of cells from renal adenocarcinoma line, renca, into Balb/c mice induces fast growing solid tumors. This method has been used to speculate the efficacy of cytokine treatment for tumor regression. The present study was designed to investigate the effect of treadmill exercise on tumor growth using renca mouse tumor model. We also examined the expression pattern of several genes related to immune response after exercise training for 4 weeks. Exercise training reduced the size of tumor mass compared to that of non-exercised group in tumor cell recipient mice. Natural killer(NK) cell activity showed no significant difference between exercised and non-exercised groups in tumor cell non-recipient mice. Although NK cell activity of non-recipients was higher than tumor cell recipients, NK cell activity of exercised group was significantly lower than non-exercised group among tumor cell recipients. There were no significant differences in mRNA expressions of cytokines. scavenger receptors, and neuropeptieds which are related to immune response, while mRNA expression of TGF-beta was significantly reduced both in spleen and in tumor mass by treadmill exercise. Based on the results presented in this study, endurance exercise has shown the possibility to suppress tumor formation.

한우에 있어 황토 첨가급여가 면역기능에 미치는 영향

조원모,기광석,정하연,강수원,김준식,오성종,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.2

본 연구는 한우 육성우에 대한 황토 첨가급여가 항병성에 미치는 효과를 구명하고자 혈액성상변화를 규명한바 아래와 같은 결과를 얻었다. 대조구 및 처리구에서 전체적인 혈액성상의 변화는 없었으나 백혈구수치가 대조구 및 처리구에서 각각 15.35K/㎕ 및 13.37K/㎕이였으며, 적혈구에 있어서도 정상수치인 5~10M/㎕보다 대조구 10.03M/㎕, 처리구 11.35M/㎕로 모두 높은 수치를 보였다. 황토첨가에 따른 혈액성상의 변화는 처리간에 유의한 차이는 보이지 않았다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the immunity of growing Hanwoo steers. A total of 8 calves were divided into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitu reddish clay) for Hanwoo steers. This study was carried out for 5 months using 8 calves. The results obtained by the present study are summarized as follows : The proportion of WBC, NE as well as LY(15.35, 6.85, 6.86K/??) was higher in the control group than in the Reddish clay-supplemented group. But no statistical differences were found in blood substances among treatment calves.

크릴분말이 콜레스테롤 및 카드뮴 식이 흰쥐의 간 및 심장 지질에 미치는 영향

조영숙,박석규,박정로,손미예,문주석,곽수동 순천대학교 기초과학연구소 1992 基礎科學硏究誌 Vol.3 No.-

In order to investigate effects of krill on lipid components of liver and heart in cholesterol and cadmium-fed rats, male Sprague-Dawley rats were raised for 4 weeks with 8 experimental diets. Krill used in this experiment contained 65.4%(w/w) of protein and 16.7% of lipid. Krill contained 35% of polyunsaturated fatty acids and more unsaturated fatty acids than saturated fatty acids by 2 times. Amino acid content of krill was high in order of Glu, Asp, Tyr and Lys. Rats fed krill diet showed more food intake and weight gain compared with control group. However, food efficiency ratio and weight of liver and heart were not significantly different. The ingestion of cadmium resulted in a severe restriction in growth rate with normal or mild reduction of liver and heart weight. Cadmium also caused a significant decrease in food efficiency ratio. The contents of total and free cholesterol of liver and heart in rats fed krill diets were similar or slightly higher than those fed control diet. Dietary krill also showed no significant difference in liver and heart cholesterol levels in rats with cadmium ingestion. The contents of phospholipid and triglyceride in liver and heart of krill group were slightly higher than those of control group. Supplement of krill reduced triglyceride content of liver in rats ingested with cadmium without any effect on the level of phospholipid and heart lipids. The concentration of cadmium in plasma significantly increased with dietary krill. However, the krill supplement did not influence the concentration of cadmium in liver or heart. A significant accumulation of lipid in liver tissue was observed in all dietary groups but standard group. However, no difference in degree of lipid accumulation was found among the dietary groups. Necrosis and hemolysis of liver in all dietary groups were not shown.

초등학교 특수학급 교사의 특수교육보조원 활용실태

정정화,조인수 大邱大學校 師範大學 附設 敎育硏究所 2009 學校敎育硏究 Vol.5 No.1

본 연구는 초등학교에 근무하는 특수학급 교사들의 특수교육 보조원 활용실태를 알아보는데 연구의 주안점을 두었다. 구체적인 연구목적은 첫째, 특수 교육 보조원 활용성과에 관한 초등학교 특수학급 교사의 인식수준을 밝히고 둘째, 초등학교 특수학급 교사들의 특수교육 보조원 활용실태를 밝히는데 있다. 얻어진 결론은 초등학교 특수학급 교사들은 특수교육보조원 제도 운영에 긍정적인 인식을 갖고 있고, 통합교육 교육을 위한 바람직한 제도이며, 학생 및 학부모 모두에게 긍정적인 효과를 내며 특수교육에 질적인 변화를 가져온다고 인식하고 있다. 활용실태 면에서 수업협의, 통합 학급 교사들과의 갈등해소, 팀 체제의 구성, 특수교육보조원에 대한 책임한계, 자질 및 역할 수행에 대한 만족도 차이와 직무연수, 선발ㆍ배치 관리에 있어 학교의 자율성, 학생 인원수에 따른 보조원수 조정, 행ㆍ재정적 지원대책 등을 분석하였다. 구체적인 인식과 실태분석에서 제시된 내용들은 특수교육 보조원의 효율적 활용방안에 기초자료를 제공해 줄 수 있을 것이다. The purposes of this study is to examine the perception of teachers for special classes in elementary schools on the outcomes of the utilization of paraprofessionals for students with disabilities, the actual use of those paraprofessionals by teachers for the use of paraprofessionals in a bid to suggest some reform measures about how to take advantage of paraprofessionals. The subjects of this study were teachers who are in charge of special classes in 60 elementary schools in Daegu. The findings of the study are as follows; First, the elementary school teachers for special classes took a positive view of the paraprofessional system. They thought that the employment of paraprofessionals contributed to laying the solid groundwork for inclusive education, and that educational services provided by them brought a change to the quality of special education. Second, after paraprofessionals started to work, students with disabilities had more opportunities to receive inclusive education as they helped them when they got around or needed another help. The teachers had a discussion with them in advance about instruction, which served to offer more practical inclusive education. To let the paraprofessional system take root, however, there should be mutual confidence between the teachers and paraprofessionals, and management and evaluation criteria should be clarified.

SOL-GEL법을 이용한 강유전체 BaTiO₃박막 및 Ceramics 제조

조채룡,이수재,장민수,김호종,정세영,노동택,김성철 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1

Concentrated solution was prepared for a sol-gel process of BaTiO₃. Barium hydroxide and Ti-isopropoxide were used as the raw materials with methanol and isopropanol as solvents. Dense ferroelectric polycrystalline BaTiO₃thin films with the perovskite structure were fabricated by spin coating of the prepared concentrated solution on a few substrates. From XRD investigation, it was confirmed that tetragonal phase of polycrystalline BaTiO₃powders are formed after heat treatment above 550℃ in air and grain-oriented BaTiO₃thin films are obtained by coating of 0.06M concentrated solution on the substrates. It was observed that surfaces of thin films deposited on the substrates by our methods were formed crack-free and uniformly. The thickness of thin film which depends the concentration of the solution was 400∼900Å for 1 time coating, which was confirmed by SEM analysis. Ceramics and thin films show dielectric constant of 4600 (at 1㎑) and capacitance 300∼800㎊ (at 1㎑) at room temperature, respectively.

HPLC를 이용한 Alnus속 식물에서의 diarylheptanoids의 정량분석

정동욱,김준식,조수민,이연아,김광호,김세원,이민원 중앙대학교 약학연구소 1999 약학 논총 Vol.13 No.-

Quantitative determination of diarylheptanoids (oregonin and hirsutanonol), which were characteristic components of Alnus spp. has been conducted by High Performance Liquid Chromatography. The HPLC analysis was carried out using an YMC J'sphere ODS-H80 column [150×4.6mm, S-4μm. 80A(particle)] and a combination of acetonitrile and H2O was used as mobile phase in gradient solvent system. The results showed differnce in the species (Alnus japonica, A hirsuta and A. hirsuta var. sibirica)

브가츠키(Vygotsky)의 사회-문화적 인지발달 이론과 수학적 의견교환

조정수 한국수학교육학회 1999 初等 數學敎育 Vol.3 No.2

The reform movements of current mathematics education have based on several major ideas, in order to provide a new vision of the teaching and learning of mathematics. Of the ideas, the motto of communication of mathematics appears to be a significant factor to change teaching practices in mathematics classroom. Through Vygotsky's sociocultural theory, the psychological background is presented for both supporting the motto and extracting important suggestions of the reform of mathematics education. The development of higher mental functions is explained by internalization, semiotic mediation. and the zone of proximal development. Above all, emphasis is put on the concepts of scaffolding and intersubjectivity related to the zone of proximal development. Seven implications are proposed by Vygotsky's sociocultural theory for the new forms of the teaching and learning of mathematics.