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배양중인 계배간충직세포의 protein kinase C 활성도 분석
朴大圭,孫鍾京,鄭在昌 경북대학교 1992 논문집 Vol.24 No.-
To investigate the relationship between protein kinse C (PKC) and chondrogenesis, PKC activity was assayed in cultures of stage 23/24 chick limb bud mesenchymal cells under various conditions. PKC activities of cytosolic and particulate fractions were low in 1 day cultured cells. As chondrogenesis proceeds, cytosolic PKC activity increased more than 2-fold, while that of the particulate fraction increased only slightly. Three days' treatment of cultures with PMA(5×10 exp (-8)M) inhibited chondrogenesis judged by the accumlation of Alcian blue bound to the extracellular matrix and depressed PKC activity in cytosolic fraction. When cells were grown for 3 days in control medium after 3 days' treatment with PMA, chondrogenesis resumed and PKC activity recovered to normal values. PKC activity in cultures plated at low density (5×10 exp (6) cells/ml) or that in PMA treated cells. On the other hand, staurosporine promoted chondrgenesis without affecting PKC activity. Furthermore, reversal of PMA's inhibitory effect on chondrogenesis by staurosporine was not accompanied by recovery of PKC activity. These data indicate that increases in PKC activity is closely related to chondrogenesis and that PMA inhibits chondrogenesis by depressing PKC. However, staurosporine's enchancing effect of chondrogenesis is not related of PKC activity.
정원우,이윤종,강용희,윤일희,이우붕,손종경,김중욱,강동진,권용주 慶北大學校 師範大學 科學敎育硏究所 1999 科學敎育硏究誌 Vol.23 No.-
The purpose of this study is to investigate the status of the experimental and practical education in high school general science. The present status and reasonable management of the experimental and practical education in high school general science were evaluated by the questionaires. To do this, 146 science teachers in Korea are administered questionaires. The frequency of the experimental and practical items in the seven kind of general science text books were investigated. The problems and the reasonable management for experimental and practical education were proposed in this paper.
Sonn,Jong Kyung,Lim,Young Bin,Yoo,Byung Je,Lee,Sun Ryung,Kang,Shin-Sung The Korea Science and Technology Center 1998 BMB Reports Vol.31 No.4
To understand the role of protein kinase C(PKC) in the regulation of chondrogenesis, we examined proteins which are phosphorylated by PKC. Stage 23/24 chick embryo wing mesenchymes were micromass-cultured to induce chondrogenesis and cell extracts were phosphorylated in a condition that activates PKC. Several proteins including 63 and 66 kDa proteins were phophorylated. The 66 kDa protein was phosphorylated only in the presence of phorbol 12-myristate 13-acetate (PMA) and phosphatidylserine (PS), and the phosphorylation was almost completely diminished by bisindolylmaleimide, a PKC inhibitor. In addition, partially purified PKC increased the phosphorylation of the 66 kDa protein. Treatment of cultures with lysophophatidylcholine (LPC) promoted chondrogenesis and phosphorylation of 66 kDa protein, while PMA adn thymeleatoxin inhibited both of the two events. Our results suggest that the 66 kDa protein is a putative substrate of PKC, and phosphorylation of the 66 kDa protein, probably by PKCα is required for chodrogenesis.
계배 wing bub 간충직세포의 연골화에 미치는 cAMP와 staurosporine의 영향
손종경 ( Jong Kyung Sonn ) 경북대학교 과학교육연구소 1992 科學敎育硏究誌 Vol.16 No.-
To investigate the mechanisms of enhancing effect by cAMP and staurosporine on chondrogenesis, cultures of HR-stage 23/24 chick embryo limb bud mesenchymal cells were treated with cAMP, staurosporine or PMA on different culture days and quantitative analysis of chondrogenesis and morphological study were employed. Staurosporine showed chondrogenic effect in any time during the culture. In the cross section of staurosporine treated micromass cul-tures, larger and denserly Alcine blue stained nodules were observed. Several other inhibitors of PKC or protein tyrosine kinase did not show any significant effect on chondrogenesis. Chondrogenesis was promoted when cAMP was added to the cultures after 1 day of culture but there was no clear nodule in cAMP treated cultures. These data indicate that staurosporine pro-moted chondrogenesis by inducing cell condensation during the early stage of differentiation and cAMP affects chondrogenesis after cell condensation possibly by modulating gap junctional com-munication.
Sonn, Chung Hee,Choi, Jong Rip,Kim, Tae-Jin,Yu, Young-Bin,Kim, Kwanghee,Shin, Suk Chul,Park, Gil-Hong,Shirakawa, Toshiro,Kim, Hee Sun,Lee, Kyung-Mi Oxford University Press 2012 Journal of radiation research Vol.53 No.6
<P>The possible beneficial effects of chronic low-dose irradiation (LDR) and its mechanism of action in a variety of pathophysiological processes such as cancer are a subject of intense investigation. While animal studies involving long-term exposure to LDR have yielded encouraging results, the influence of LDR at the cellular level has been less well defined. We reasoned that since natural killer (NK) cells constitute an early responder to exogenous stress, NK cells may reveal sentinel alterations in function upon exposure to LDR. When purified NK cells received LDR at 4.2 mGy/h for a total of 0.2 Gy <I>in vitro</I>, no significant difference in cell viability was observed. Likewise, no functional changes were detected in LDR-exposed NK cells, demonstrating that LDR alone was insufficient to generate changes at the cellular level. Nonetheless, significant augmentation of cytotoxic, but not proliferative, function was detected when NK cells were stimulated with low-dose IL-2 prior to irradiation. This enhancement of NK cytotoxicity was not due to alterations in NK-activating receptors, NK1.1, NKG2D, CD69 and 2B4, or changes in the rate of early or late apoptosis. Therefore, LDR, in the presence of suboptimal cytokine levels, can facilitate anti-tumor cytotoxicity of NK cells without influencing cellular proliferation or apoptosis. Whether these results translate to <I>in vivo</I> consequences remains to be seen; however, our data provide initial evidence that exposure to LDR can lead to subtle immune-enhancing effects on NK cells and may explain, in part, the functional basis underlying, diverse beneficial effects seen in the animals chronically exposed to LDR.</P>
계배 wing bud 간충직세포의 연골화에 미치는 cAMP와 staurosporine의 영향
孫鍾京 경북대학교 과학교육연구소 1992 科學敎育硏究誌 Vol.16 No.-
To investigate the mechanisms of enhancing effect by cAMP and staurosporine on chondrogenesis, cultures of HH-stage 23/24 chick embryo limb bud mesenchymal cells were treated with cAMP, staurosporine or PMA on different culture days and quantitative analysis of chondrogenesis and morphological study were employed. Staurosporine showed chondrogenic effect in any time during the culture. In the cross section of staurosporine treated micromass cultures, larger and denserly Alcine blue stained nodules were observed. Several other inhibitors of PKC or protein tyrosine kinase did not show any significant effect on chondrogenesis. Chondrogenesis was promoted when cAMP was added to the cultures after 1 day of culture but there was no clear nodule in cAMP treated cultures. These data indicate that staurosporine promoted chondrogenesis by inducing cell condensation during the early stage of differentiation and cAMP affects chondrogenesis after cell condensation possibly by modulating gap junctional communication.