In Vitro Screening for Compounds Derived from Traditional Chinese Medicines with Antiviral Activities Against Porcine Reproductive and Respiratory Syndrome Virus

( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.

The Influence of Regulatory Focus on Individual Innovation Performance: Focused on the Mediating Effect of Employees’ Creativity and the Moderating Effect of Creative Self-Efficacy

Guo, Song(곽송),Chung, Soo-Jin(정수진) 한국경영교육학회 2020 경영교육연구 Vol.35 No.3

[연구목적] 본 연구에서는 종업원 혁신성과를 효율적으로 개선하는 방법에 대한 문제를 바탕으로 조절초점과 종업원 혁신성과의 관계를 살펴보고 종업원의 창의성이 이들 사이에서의 매개효과와 창의적 자기 효능감의 조절 효과를 검증하여 조직관리에 이론적 근거와 의견을 주고자 한다. [연구방법] 이론적 연구를 바탕으로 본 논문은 4가지 연구 가설을 제시하고 연구 모델을 수립하여 설문조사 방식으로 중국 조직 구성원을 대상으로 실증분석하고SPSS와 AMOS로 연구 모델의 가설을 검증하였다. [연구결과] 조절초점은 종업원 혁신성과에 상당한 영향을 미치고 있으며 종업원의 창의성과 창의적 자기 효능감은 조절초점 혁신성과 관계에 있어 중간 매개효과와 조절효과를 하고 있다. [연구의 시사점] 관리자는 직원의 주체작용을 충분히 발휘하여 종업원의 창의성을 향상시키도록 노력해야 하며 또 적극적으로 종업원의 창의적 자기 효능감을 키워 혁신의 성과를 이루도록 해야 한다. [Purpose] The study begins with the practical problem of how to effectively use the individual differences on regulatory focus, avoiding its negative effects as far as possible and maximizing its positive effects, so as to promote more innovative behaviors among employees. Therefore, it verifies the effect of regulatory focus on individual innovation performance, the mediating effect of employees’ creativity and the moderating effect of creative self-Efficacy. [Methodology] Base on theoretical research, this study puts forward 4 key research hypotheses and established a research model. Data were collected from employees in China by the survey method and SPSS & AMOS programs were used to test the hypotheses of the research model. [Findings] Regulatory focus is significantly affected individual innovation performance. Employees’ creativity as the mediating variable and Creative Self-Efficacy as the moderating variable effect regulatory focus and individual innovation performance. [Implications] Managers should give full play to the principal role of employees, striving to cultivate and improve employees’ creativity and Creative Self-Efficacy, so as to achieve the goal of improving individual innovation performance.

TETRAVALENT SYMMETRIC GRAPHS OF ORDER 9p

Song-Tao Guo,Yan-Quan Feng 대한수학회 2012 대한수학회지 Vol.49 No.6

A graph is symmetric if its automorphism group acts transi-tively on the set of arcs of the graph. In this paper, we classify tetravalent symmetric graphs of order 9p for each prime p.

Combining Non-Contrast CT Signs With Onset-to-Imaging Time to Predict the Evolution of Intracerebral Hemorrhage

Song Lei,Qiu Xiaoming,Zhang Cun,Zhou Hang,Guo Wenmin,Ye Yu,Wang Rujia,Xiong Hui,Zhang Ji,Tang Dongfang,Zou Liwei,Wang Longsheng,Yu Yongqiang,Guo Tingting 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.2

Objective: This study aimed to determine the predictive performance of non-contrast CT (NCCT) signs for hemorrhagic growth after intracerebral hemorrhage (ICH) when stratified by onset-to-imaging time (OIT). Materials and Methods: 1488 supratentorial ICH within 6 h of onset were consecutively recruited from six centers between January 2018 and August 2022. NCCT signs were classified according to density (hypodensities, swirl sign, black hole sign, blend sign, fluid level, and heterogeneous density) and shape (island sign, satellite sign, and irregular shape) features. Multivariable logistic regression was used to evaluate the association between NCCT signs and three types of hemorrhagic growth: hematoma expansion (HE), intraventricular hemorrhage growth (IVHG), and revised HE (RHE). The performance of the NCCT signs was evaluated using the positive predictive value (PPV) stratified by OIT. Results: Multivariable analysis showed that hypodensities were an independent predictor of HE (adjusted odds ratio [95% confidence interval] of 7.99 [4.87–13.40]), IVHG (3.64 [2.15–6.24]), and RHE (7.90 [4.93–12.90]). Similarly, OIT (for a 1-h increase) was an independent inverse predictor of HE (0.59 [0.52–0.66]), IVHG (0.72 [0.64–0.81]), and RHE (0.61 [0.54– 0.67]). Blend and island signs were independently associated with HE and RHE (10.60 [7.36–15.30] and 10.10 [7.10–14.60], respectively, for the blend sign and 2.75 [1.64–4.67] and 2.62 [1.60–4.30], respectively, for the island sign). Hypodensities demonstrated low PPVs of 0.41 (110/269) or lower for IVHG when stratified by OIT. When OIT was ≤ 2 h, the PPVs of hypodensities, blend sign, and island sign for RHE were 0.80 (215/269), 0.90 (142/157), and 0.83 (103/124), respectively. Conclusion: Hypodensities, blend sign, and island sign were the best NCCT predictors of RHE when OIT was ≤ 2 h. NCCT signs may assist in earlier recognition of the risk of hemorrhagic growth and guide early intervention to prevent neurological deterioration resulting from hemorrhagic growth.

TETRAVALENT SYMMETRIC GRAPHS OF ORDER 9p

Guo, Song-Tao,Feng, Yan-Quan Korean Mathematical Society 2012 대한수학회지 Vol.49 No.6

A graph is symmetric if its automorphism group acts transitively on the set of arcs of the graph. In this paper, we classify tetravalent symmetric graphs of order $9p$ for each prime $p$.

BOTTOM-UP MODEL FOR THE FORMATION OF GMC'S

SONG GUO-XUAN The Korean Astronomical Society 1996 Journal of The Korean Astronomical Society Vol.29 No.suppl1

A bottom-up model for the formation of GMCs is described, where the observed GMCs are the aggregates of less massive clouds. The aggregates are getting more and more massive in the process of consecutive collision between clouds.

Noncontrast Computed Tomography-Based Radiomics Analysis in Discriminating Early Hematoma Expansion after Spontaneous Intracerebral Hemorrhage

Song Zuhua,Guo Dajing,Tang Zhuoyue,Liu Huan,Li Xin,Luo Sha,Yao Xueying,Song Wenlong,Song Junjie,Zhou Zhiming 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.3

Objective: To determine whether noncontrast computed tomography (NCCT) models based on multivariable, radiomics features, and machine learning (ML) algorithms could further improve the discrimination of early hematoma expansion (HE) in patients with spontaneous intracerebral hemorrhage (sICH). Materials and Methods: We retrospectively reviewed 261 patients with sICH who underwent initialNCCT within 6 hours of ictus and follow-up CT within 24 hours after initial NCCT, between April 2011 and March 2019. The clinical characteristics, imaging signs and radiomics features extracted from the initial NCCT images were used to construct models to discriminate early HE. A clinical-radiologic model was constructed using a multivariate logistic regression (LR) analysis. Radiomics models, a radiomics-radiologic model, and a combined model were constructed in the training cohort (n = 182) and independently verified in the validation cohort (n = 79). Receiver operating characteristic analysis and the area under the curve (AUC) were used to evaluate the discriminative power. Results: The AUC of the clinical-radiologic model for discriminating early HE was 0.766. The AUCs of the radiomics model for discriminating early HE built using the LR algorithm in the training and validation cohorts were 0.926 and 0.850, respectively. The AUCs of the radiomics-radiologic model in the training and validation cohorts were 0.946 and 0.867, respectively. The AUCs of the combined model in the training and validation cohorts were 0.960 and 0.867, respectively. Conclusion: NCCT models based on multivariable, radiomics features and ML algorithm could improve the discrimination of early HE. The combined model was the best recommended model to identify sICH patients at risk of early HE.

The Studying and Simulating Analysis of the Power Harmonic Measuring Method Based on the Blind Source Analysis

Guo Song-Lin,Wang Jing-Yu,Liu Fu-Kai 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.6

Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis

Xue Song,Jing Guo,Wen-xiu Ma,Zhi-yuan Ji,Li-fang Zou,Gong-you Chen,Hua-song Zou 한국미생물학회 2015 The journal of microbiology Vol.53 No.5

To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis revealed that each of the mutants carried a single copy of Tn5. The flanking sequence was identified by plasmid rescue and 18 different ORFs were identified in the genome sequence. Of these 18 ORFs, seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis. Real-time PCR analysis showed that the seven genes were upregulated when the bacteria were grown in citrus plants, suggesting that the expression of these genes was essential for canker development.

Fault Location Scheme for Over-Zone Feeding Operation Condition on High-Speed Railway

Lin Guo-song,Quan Wei,Tong Xiao-yang 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.2

Cross-coupling autotransformer (AT) feeding system is applied on high-speed electrifi ed railways in China. According to operation requirement, ‘over-zone feeding operation condition’ exists. When fault occurs on this operation condition, the fault location system should adjust its original scheme to adapt. We analyzed the shortcoming in traditional fault location of this feeding mode. Analysis indicates that when fault occurs in the non-over-zone section, fault location can be well done by existing principle and when fault occurs in the over-zone section, a new fault location scheme must be proposed. Based on the analysis of circuit networks of over-zone section in this feeding mode, the feeder current ratio of up line and down line in sectioning post (SP) is derived when fault occurs in section from SP to AT post 2 (ATP2) and the port impedance at ATP2 is derived when fault occurs in section from ATP2 to substation 2 (SS2). A reasonable fault location scheme was proposed for over-zone section based on feeder current ratio method of up line and down line and port impedance method in this paper. The simulation results based on Matlab/Simulink showed that when fault occurs on over-zone section the fault position can be located accurately according to this novel fault location scheme