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중국어 동사 전 목적명사구 및 수동형태소 명사구의 예외적 양태와 그 함축의미
송홍기(Sohng Hong-Ki) 한국생성문법학회 2005 생성문법연구 Vol.15 No.3
It is a well-known linguistic fact that in general, only a subject can serve as an antecedent and a blocker in the case of Chinese monomorphemic reflexivization. Contrary to our expectation, a DP that follows the passive morpheme bei or the preverbal object marker, which is a non-subject, serves as an antecedent for the Chinese reflexive, even though not serving as a blocker for a higher antecedent. Sohng (2004a) shows that the blocking effect for the Chinese monomorphemic reflexive is accounted for in terms of LF Spec-head agreement that assigns and checks the φ-features of ziji. Based on LF head movement of a monomorphemic reflexive and checking of a [Refl] feature in Agr, the analysis put forward in this paper shows clearly that the unexpected non-subject antecedence on ziji is well explained in the split-I phrase structure originating with Pollock(1989) in terms of the Conditions on Subject Orientation and LF Spec-head agreement. Thus, the non-subject antecedence on ziji that this paper deals with provides firm support to the analysis given in this paper.
Glycosylation of free sterol by whole-cell bioconversion in E. coli
Jae Kyung Sohng,Jae Kyung Sohng 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Steryl glucosides play important roles in many physiological and biochemical process in organism such as the heat shock, enhancement of immunological system, etc. The alignment of a putative sterol glucosides isolated from S. tropica CNB-440 has been shown 34%, 42% and 57% in homology with the corresponding ones from Arabidopis thaliana, Avena sativa and Salinispora arenicola CNS-205, respectively. Engineered E. coli host-high level production of the UDP-glucose was used for whole-cell bioconversion of free sterol (cholesterol and β-sistosterol) and the production of glycosylated product was only detected with β-sitosterol as substrate.
Sohng, Hee Yon,Kuniyuki, Alan,Edelson, Jane,Weir, Rosy Chang,Song, Hui,Tu, Shin-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12
Background: Understanding and enhancing change capabilities, including Practice Adaptive Reserve (PAR), of Community Health Centers (CHCs) may mitigate cancer-related health disparities. Materials and Methods: Using stratified random sampling, we recruited 232 staff from seven CHCs serving Asian Pacific Islander communities to complete a self-administered survey. We performed multilevel regression analyses to examine PAR composite scores by CHC, position type, and number of years worked at their clinic. Results: The mean PAR score was 0.7 (s.d. 0.14). Higher scores were associated with a greater perceived likelihood that clinic staff would participate in an evidence-based intervention (EBI). Constructs such as communication, clinic flow, sensemaking, change valence, and resource availability were positively associated with EBI implementation or trended toward significance. Conclusions: PAR scores are positively associated with perceived likelihood of clinic staff participation in cancer screening EBI. Future research is needed to determine PAR levels most conducive to implementing change and to developing interventions that enhance Adaptive Reserve.
Sohng, Jae-Kyung,Oh, Tae-Jin,Kim, Chun-Gyu Korean Society for Biochemistry and Molecular Biol 1998 Journal of biochemistry and molecular biology Vol.31 No.5
Many antibiotics contain partially deoxygenated sugar components that are usually essential for biological activity, affinity, structural stability, and solubility of antibiotics. Gene probes of the biosynthetic genes related with the deoxysugar were obtained from PCR. Primers were designed from the conserved peptide sequences of the known dTDP-D-glucose 4,6-dehydratases, which are the key step enzymes in the biosynthesis of deoxysugar. The primers were applied to amplify parts of dehydratase genes to 27 actinomycetes that produce the metabolites containing deoxysugar as structural constituents. About 180 and 340 bp DNA fragments from all of the actinomycetes were produced by PCR and analyzed by Southern blot and DNA sequencing. The PCR products were used as gene probes to clone the biosynthetic gene clusters for the antibiotic mithramycin, rubradirin, spectinomycin, and elaiophyrin. This method should allow for detecting of the biosynthetic gene clusters of a vast array of secondary metabolites isolated from actinomycetes because of the widespread existence of deoxysugar constituents in secondary metabolites.
Production, Isolation and Biological Activity of Nargenicin from Nocardia sp. CS682
Sohng, Jae-Kyung,Yamaguchi, Tokutaro,Seong, Chi-Nam,Baik, Keun-Sik,Park, Seong-Chan,Lee, Hyo-Jeong,Jang, So-Young,Simkhada, Jaya Ram,Yoo, Jin-Cheol 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.10
Culture broth of an actinomycete isolate, Nocardia sp. CS682 showed specifically higher antibacterial activity against methicilin resistant Staphylococcus aureus (MRSA). Purified substance from the organism, CS-682, which is active against MRSA and Micrococcus leuteus, is a $C_{28}H_{37}NO_8$ ($M+H^+$, observed: 516.83) and identified as an unusual macrolide antibiotic, nargenicin. The chemical structure of CS-682 was identified by FT-IR, $^1H$-NMR, $^{13}C$-NMR, and ($^1H-^1H$ and $^1H-^{13}H$) COSY. The anti-MRSA activity of CS-682 was stronger than that of oxacillin, vancomycin, monensin, erythromycin, and spiramycin. Phylogenetic analysis showed that strain CS682 is closely related to Nocardia tenerifensis DSM $44704^T$ (98.7% sequence similarity), followed by N. brasiliensis ATCC $19296^T$ (98.4% sequence similarity). The ability of Nocardia sp. CS682 to produce nargenicin was unique.
Sohng, Jae-Kyung,Yoo, Jin-Cheol Korean Society for Biochemistry and Molecular Biol 1996 Journal of biochemistry and molecular biology Vol.29 No.3
DNA fragments, homologous to the dTDP-D-glucose 4,6-dehydratase gene, obtained from the genomic DNA of Streptomyces antibioticus $T\ddot{u}99$, a producer of the unusual macrolide antibiotic chlorothricin, were cloned and sequenced. This dehydratase gene was designated as oxil. The coding region of the oxil gene is composed of 987 bp, and analysis of the DNA sequence data reveals sequences for the gene products of 329 amino acids (molecular weight of 36,037). The deduced amino acids are 59% identical to the StrE, dTDP-D-glucose 4,6-dehydratase from the streptomycin pathway. The oxil's function was examined by expressing it in E. coli using the T7 RNA polymerase/promoter system (pRSET) to produce an active fusion protein including a his tag. This enzyme shows specificity of substrate, specific only to dTDP-D-glucose.
Intermodulation Distortion in Variable-Resistance Diodes
Sohng, Kyu-Ik 경북대학교 공과대학 1983 工大硏究誌 Vol.12 No.-
可變抵抗다이오우드의 찌그러짐을 Volterra급수전개를 利用하여 解析하였다. 可變抵抗다이오우드의 intermodulation distortion에 對한 理論的 表現式을 유도하고, 實驗으로써 그 타당성을 입증하였다. 特히 2개의 다이오우드를 접속할 경우에 對한 찌그러짐을 解析하였으며, 다이오우드의 back-to-back 접속에 의한 찌그러짐의 현저한 減少가 나타남을 알았다.