Association between a p73 Gene Polymorphism and Genetic Susceptibility to Non-small Cell Lung Cancer in the South of China

Wang, Shuang-Shuang,Guo, Hai-Yan,Dong, Lin-Li,Zhu, Xiang-Qian,Ma, Liang,Li, Wen,Tang, Jian-Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: This study aimed to identify any association between the p73 gene G4C14-to-A4T14 polymorphism and risk of non-small cell lung cancer (NSCLC) in the south of China. Materials and Methods: We genotyped the p73 gene polymorphism of peripheral blood DNA from 168 patients with NSCLC and 195 normal controls using HRM (high resolution melting) and PCR-CTPP (polymerase chain reaction with confronting two-pair primers). Results: The results of genotyping by HRM and PCR-CTPP were consistent with direct sequencing, the p73 genotype distribution in 168 lung cancer patients being as follows: GC/GC 101 cases (60.1%), GC/AT 59 cases (35.1%), AT/AT 8 cases (4.8%). The carriers of AT/AT genotype had a significantly reduced risk of NSCLC (OR=0.370; 95%CI: 0.170-0.806; p=0.010) as compared with non-carriers. However, we found no relations between p73 genotypes and histological type (p=0.798, $x^2=0.452$), tumor stage (p=0.806, $x^2=0.806$), or lymph node metastasis (p=0.578, $x^2=1.098$). Conclusions: Our findings suggest that the p73 G4C14-to-A4T14 polymorphism may be a modifier of NSCLC susceptibility in the Chinese population.

애니메이션 영화 〈코코(Coco)〉에서 표현된 주제곡 음악적 기능 분석

장샤오솽(Zhang xiao-shuang),샤오린(Xiao lin),최동혁(Choi dong-hyuk),이현석(Lee hyun-seok) 한국콘텐츠학회 2019 한국콘텐츠학회 종합학술대회 논문집 Vol.2019 No.5

Two general odorant binding proteins display high bindings to both host plant volatiles and sex pheromones in a pyralid moth Chilo suppressalis (Lepidoptera: Pyralidae)

Sajjad Ali Khuhro,Hui Liao,Xiao-Tong Dong,Qi Yu,Qi Yan,Shuang-Lin Dong 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Odorant sensitivity and selectivity play a crucial role in insects for finding host plants,mates and oviposition sites. General odorant binding proteins (GOBPs) are thought to be involved in the detection of general odorants released by foods and host plants. However, recent studies suggest GOBPs may also play some role in detection of sex pheromones. In the present study with the rice striped stem borer Chilo suppressalis (Walker) real time quantitative PCR (qPCR) measurements showed that both GOBP genes were specifically expressed in the antennae of both sexes, proposing their important roles in olfaction. To examine their specific functions, binding affinities to three sex pheromones and 35 plant volatiles were measured using fluorescence competitive binding assays. As a result, both GOBPs showed very strong binding affinities (Ki=0.33–1.50 μM) to all three sex pheromone components (Z11–16:Ald, Z9–16:Ald and Z13–18:Ald). In addition, GOBP1 and GOBP2 displayed high binding affinities (Ki b 10 μM) to two volatiles (farnesol and oleic acid) and five volatiles (+)-cedrol, farnesol, myrcene, β-ionone and linoleic acid), respectively; GOBP1 and GOBP2 also displayed moderate binding affinities (Ki = 10–20 μM) to two and one compounds, respectively. Furthermore, electroantennogram (EAG) measurements indicated that except oleic acid and myrcene, all volatiles of high binding affinity could elicit significant EAG response in moths of one or both sexes. Our study suggests that two CsupGOBPs tune to different array of plant volatiles, and also play roles in perception of the female sex pheromones, providing insights in to the mechanisms of olfaction in C. suppressalis.

Chemical analysis of the female sex pheromone in Palpita nigropunctalis (Lepidoptera: Crambidae)

Qi Yan,Aguri Fujino,Hideshi Naka,Shuang-Lin Dong,Tetsu Ando 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4

The lilac pyralid, Palpita nigropunctalis Bremer (Lepidoptera: Crambidae), is a common pest of Oleaceae plants. A crude extract of the female sex pheromone glands was examined by gas chromatography-electroantennogram detection (GC-EAD) and GC coupled to a mass spectrometer (GC/MS). The GC-EAD analysis revealed three EAGactive components (I–III) in a ratio of 1:0.2:0.01 (I: II: III). GC/MS analysis successfully recorded the mass spectra of I and II. For I, ions at m/z 238 (M + ) and 220 ([M-18] + ) indicated the structure of a monoenyl aldehyde with a 16-carbon chain. For II, M + was not detected, but ions at m/z 222 ([M-60] + ) and 61 ([AcOH +1] + ) suggested that II was a monoenyl acetate with a 16-carbon chain. Further GC/MS analysis of the extract treated with dimethyl disulfide revealed that the double bonds in both I and II are located at the same position of 11th-carbon. In addition, the pheromone extract was examined by GC/Fourier transform-infrared spectrophotometer (GC/FT-IR). An IR spectrum of I showed characteristic absorption at 1716 and 966 cm −1 , indicating a formyl group and E configuration of the double bond, respectively. In the case of II, absorption at 1745 and 968 cm −1 indicated an ester carbonyl and E configuration, respectively. Taken together and by comparison with authentic standards, I and II were confirmed as (E)-11-hexadecenal and (E)-11-hexadecenyl acetate, respectively; while III was speculated as (E)-11-hexadecen-1-ol. The synthetic I, II and III all coincided well with those of the natural components in chemical data, and elicited strong electroantennographic activity in male P. nigropunctalis.

Functionalization Modification of the Fischer-Tropsch Wax to Improve the Mechanical and Crystallization Properties of the Recycled Polypropylene/Attapulgite Composites

Tian-Jiao Zhao,Fu-Hua Lin,Shuang-Dan Mao,Ya-Peng Dong,Jia-Le Zhao,Wen-Ju Cui,Shu-Hui Wang,Ding-Yi Ning,Jing-Qiong Lu,Bo Wang 한국고분자학회 2024 폴리머 Vol.48 No.3

The attapulgite (ATP) with unique one-dimensional rod-like structure was used to improve the mechanical and crystallization properties of the recycled polypropylene (rPP). In order to increase the compatibility of the polar ATP and the non-polar rPP, the maleic anhydride (MAH) was grafted onto the fischer-tropsch wax (FTW) then reacted with ATP to prepare FATP. The tensile, flexural and impact strength of the composites were maximized at the FATP content was 3 wt%, increased by 13.09, 17.56, and 101.92% compared with rPP, and reaching the level of the PP. Due to the improved compatibility, the mechanical properties of the rPP/FATP composites were increased compared with the rPP/ATP composites at the same addition. Moreover, the differential scanning calorimetry (DSC) analysis proved that the addition of the ATP or FATP improved the crystallization properties of the rPP. The crystallization temperature (Tp) of the rPP, rPP/3 wt% ATP, and rPP/3 wt% FATP was 126.31, 127.86, and 129.37 ℃, respectively. The non-isothermal and isothermal crystallization kinetic results showed that the crystallization rate was increased with the addition of the ATP and FATP. Meanwhile, by reason of the synergistic effect of compatibility improvement and internal lubrication, the crystallization rate of the rPP/FATP composites was faster than the rPP/ATP composites. However, the ATP or FATP added in rPP had no effect on the spherulites growth mode of the composites. Above all, the scanning electron micrographs (SEM) results provided intuitive evidence which the compatibility between FATP and rPP was greatly improved.

Tissue distribution and functional characterization of odorant binding proteins in Chilo suppressalis (Lepidoptera: Pyralidae)

Sajjad Ali Khuhro,Hui Liao,Guan-Heng Zhu,Shuang-Mei Li,Zhan-Feng Ye,Shuang-Lin Dong 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

Odorant binding proteins (OBPs) play important roles in the insect olfaction and other diverse physiological processes. Forty OBP genes have been molecularly identified from Chilo suppressalis (Walker), a notorious rice pest in Asian countries, but little is known about the olfactory function for most of these genes. In the present study, we first determined the tissue expression profiles of 34 OBPs (excluding two general odorant bonding proteins (GOBPs) and four pheromone binding protein (PBPs)) by quantitative real-time PCR (qPCR), and found that 9 genes (OBP1, 3, 4, 11, 15, 17, 19, 20 and 24) were specifically or predominantly expressed in antennae of both sexes, suggesting their roles in olfaction, while three genes (OBP29, 30 and 32) were almost not expressed in antennae. Focusing on olfactory roles, the ligand specificities of six antenna specifically or predominantly expressed genes were further investigated for 35 plant volatiles, using the fluorescence competitive binding assays. The results revealed that six OBPs displayed different ligand preference, suggesting a differentiation of OBPs in ligand binding spectrum. Of six tested OBPs, OBP3, 11, 17, 19 and 31 showed moderate (Ki =10.21–19.85 μM) or high (Ki < 10.00 μM) binding affinity for 11 and one plant volatiles, respectively. In particular, a plant volatile β-ionone had high or moderate binding to all five OBPs. Our study suggests that these five OBP genes play important roles in the perception of different host plant volatiles, providing insight into the olfactory mechanism in C. suppressalis.

Detection and Characterization of PCR-SSCP Markers of the Bovine Lactoferrin Gene for Clinical Mastitis

Zhou, Lei,Yang, Yuan-Yuan,Li, Zhong-Hao,Kong, Li-Juan,Xing, Guan-Dong,Di, He-Shuang,Wang, Gen-Lin Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.10

A total of 80 cows, including 40 top mastitis resistant and 40 top mastitis susceptible animals as Group I and Group II, were selected from a population of 520 cows based on clinical mastitis occurrence. PCR-SSCP analysis on four fragments within the 5'region and two fragments of Exons 4,15 of bovine lactoferrin (bLF) revealed that four fragments-P1,P4,E4,E15-had polymorphisms which totally included six base mutations, and only two of them had significant differences in allele frequencies between resistant and susceptible groups, P1 (53.7% vs. 70.0%, p<0.05) and P4 (55.0% vs. 68.8%, p<0.05). Further study on these two promising markers combined with the milk performance traits of cows demonstrated that their selection would result in higher fat percentage (p<0.05), lower Somatic Cell Score (SCS) (p<0.05) and Clinical Mastitis Residuals (CMR) (p<0.01) indicating higher mastitis resistance and lower milk yield (p<0.05). The putative transcription factor binding sites in the 5'region were also studied by using MatInspector 7.2.2 software, and two signal pathways regulating the expression of bLF including the NF-${\kappa}B$ pathway and nuclear hormone receptor pathway were predicted.