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RISS 인기검색어
- 검색키워드
- 저자 : Shringi, S. (검색결과 5 건)
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Smriti Shringi,B.N. Shringi 대한수의학회 2005 Journal of Veterinary Science Vol.6 No.1
The sero-prevalence of antibodies against blue tongue virus (BTV) in 408 local breeds of sheep in Rajasthan state in India was investigated using standard agar gel immunodiffusion (AGID) test. Maximum seropositivities of 11.3% (13/115), 10.7% (13/121), 7.1% (11/155) and 5.9% (1/17) were recorded in the Chokla, Magra, Nali and Pugal breeds, respectively. Out of 107 goat serum samples, 6 (5.6%) were AGID positive. The performance of the standard AGID, counter current immuno-electrophoresis (CCIE) and the competitive enzyme-linked immunosorbent assay (cELISA) for the detection of serum antibody against BTV in indigenous breeds of sheep were compared. Out of 178 sheep serum samples tested, 17 (9.5%), 22 (12.3%) and 54 (30.3%) were positive for group-specific bluetongue antibodies by AGID, CCIE and cELISA, respectively. There was appreciable difference in the seroprevalence detected by AGID, CCIE and cELISA in clinically healthy and diseased sheep with regard to relative sensitivities and specificities of the tests with cELISA being highly sensitive and specific followed by CCIE and AGID test. It was concluded that these indigenous breeds of sheep may be a potential reservoir of BTV infection and cELISA should be routinely used for the detection of antibodies against BTV in these local breeds of sheep
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Shah, D.H.,Shringi, S.,Desai, A.R.,Heo, E.J.,Park, J.H.,Chae, J.S. Elsevier Scientific Pub. Co 2007 Veterinary microbiology Vol.119 No.2
Salmonella enterica serotype Gallinarum (S. Gallinarum) is the causative agent of fowl typhoid (FT) in chickens. FT is a severe systemic disease of chickens causing heavy economic losses to the poultry industry through mortality, reduced egg production and culling of precious breeding stocks. In this study, a metC (encoding cystathionine β lyase) mutant was produced from a virulent strain of S. Gallinarum by Mini-Tn5 insertional inactivation. The mutant was significantly attenuated in virulence for 1-day-old White Leghorn chickens. Inactivation of metC resulted in 10<SUP>4</SUP>-fold increase in the LD<SUB>50</SUB> when compared with the wild type parent. The metC mutant showed an in vivo competitiveness defect in the challenged chickens and significantly lower (P<0.01) bacterial burden in the reticuloendothelial organs when compared with the wild-type parent. These results indicate that metC gene is important for virulence of S. Gallinarum in chickens.
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An Algorithm for Splitting a Box by a Loop and Its Applications in Manufacturing
Kheerwal, Anoop,Shanmuganathan, Vivekananda,Shringi, Rohitashwa,Karunakaran, Karuna P. Society for Computational Design and Engineering 2003 International Journal of CAD/CAM Vol.3 No.1
During the design of dies and molds, the cavity of the object is obtained by subtracting it from a surrounding rectangular block. This box is subsequently split into two halves by the parting surface. Similar problems also occur in some RP processes such as LOM, SGC, SLS and 3DP where the machine produces a block inside which the prototype is buried. Determining the orientation of the object inside the box and the corresponding parting surface taking appropriate constraints into account have been addressed by several researchers. However, given the parting surface, the problem of splitting the box development of a software package called OptiLOM (now a module of an RP software Magics 8.0), the authors realized non-triviality of this problem since the loop can spread over as many as 5 faces of the box. In this paper, the authors have tried to bring out the importance of this problem and have presented their algorithm to solve it.
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Phenotypic Diversity of Escherichia coli O157:H7 Strains Associated with the Plasmid O157
Ji Youn Lim,홍준배,Haiqing Sheng,Smriti Shringi,Thomas E. Besser,Carolyn J. Hovde,Rajinder Kaul 한국미생물학회 2010 The journal of microbiology Vol.48 No.3
Escherichia coli O157:H7, a food-borne pathogen, causes hemorrhagic colitis and the hemolytic-uremic syndrome. A putative virulence factor of E. coli O157:H7 is a 60-MDa plasmid (pO157) found in 99% of all clinical isolates and many bovine-derived strains. The well characterized E. coli O157:H7 Sakai strain (Sakai) and its pO157-cured derivative (Sakai-Cu) were compared for phenotypic differences. Sakai-Cu had enhanced survival in synthetic gastric fluid, did not colonize cattle as well as wild-type Sakai, and had unchanged growth rates and tolerance to salt and heat. These results are consistent with our previous findings with another E. coli O157:H7 disease outbreak isolate ATCC 43894 and its pO157-cured (43894-Cu). However, despite the essentially sequence identical pO157 in these strains, Sakai-Cu had changes in antibiotic susceptibility and motility that did not occur in the 43894-Cu strain. This unexpected result was systematically analyzed using phenotypic microarrays testing 1,920 conditions with Sakai, 43894, and the plasmid-cured mutants. The influence of the pO157 differed between strains on a wide number of growth/survival conditions. Relative expression of genes related to acid resistance (gadA, gadX, and rpoS) and flagella production (fliC and flhD) were tested using quantitative real-time PCR and gadA and rpoS expression differed between Sakai-Cu and 43894-Cu. The strain-specific differences in phenotype that resulted from the loss of essentially DNA-sequence identical pO157 were likely due to the chromosomal genetic diversity between strains. The O157:H7 serotype diversity was further highlighted by phenotypic microarray comparisons of the two outbreak strains with a genotype 6 bovine E. coli O157:H7 isolate, rarely associated with human disease.
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Microbial pathogens in ticks, rodents and a shrew in northern Gyeonggi-do near the DMZ, Korea
Joon-Seok Chae,유도현,Smriti Shringi,Terry A. Klein,Heung-Chul Kim,Sung-Tae Chong,In-Yong Lee,Janet Foley 대한수의학회 2008 Journal of Veterinary Science Vol.9 No.3
A total of 1,618 ticks [420 individual (adults) and pooled (larvae and nymphs) samples], 369 rodents (Apodemus agrarius, Rattus norvegicus, Tscherskia triton, Mus musculus, and Myodes regulus), and 34 shrews (Crocidura lasiura) that were collected in northern Gyeonggi-do near the Demilitarized Zone (DMZ) of Korea during 2004-2005, were assayed by PCR for selected zoonotic pathogens. From a total of 420 individual and pooled tick DNA samples, Anaplasma (A.) phagocytophilum (16), A. platys (16), Ehrlichia (E.) chaffeensis (63), Borrelia burgdorferi (16), and Rickettsia spp. (198) were detected using species-specific PCR assays. Out of 403 spleens from rodents and shrews, A. phagocytophilum (20), A. platys (34), E. chaffeensis (127), and Bartonella spp. (24) were detected with species-specific PCR assays. These results suggest that fevers of unknown causes in humans and animals in Korea should be evaluated for infections by these vector-borne microbial pathogens.
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