지하수위에 따른 적양배추, 브로콜리, 양상추의 수분이용효율 특성

신수정, 홍순달 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

자궁적출술을 경험한 여성의 통증과 냉증완화를 위한 쑥뜸요법 효과의 예비연구

신경림,곽순애,신수진 성인간호학회 2001 성인간호학회지 Vol.13 No.1

The Purpose of this pilot study was to identify the effects of hand moxibustion therapy to decrease pain and relieve coldness of the body in women who had a hysterectomy. The conceptual framework of this study was derived from Ying-Yang, Khi and other corresponding theories. The data were collected from February to May 1997 A Graphic Rating scale was used to measure the degree of pain and Digital Infrared Thermographic Imaging(D.I.T.I) was used to examine the degree of coldness. The subjects were women who resided in Seoul and had a hysterectomy within five years. An experimental group was composed of five women who received moxibustion and a control group was composed of five who did not. The research procedure began with having both the experimental group and control group describe their general characteristics and the degree of pain they perceived. Then, Digital Infrared Thermographic Imaging(D.I.T.I) was conducted. Moxibustion was only given to the experimental group on both hands twice every day for a total of five weeks. Their perception of pain and D.I.T.I were examined weekly. The control group received no therapy. After finishing therapy. the perception of pain and D.I.T.I for both groups were also measured. According to this study, moxibustion therapy resulted in a change of body temperature on the right shoulder joint(p=0.00074), abdomen(p=0.0047), waist(p=0.0068) and hands(p=0.0317) respectively. Also, the study results showed significant decrease (p=0.0001) in pain over time and significant improvement over body coldness.

광주광역시 치매의 유병률과 위험인자의 도시-농촌 지역 비교

신일선,김재민,윤진상,김석재,양수진,김웅장,이승현,강순아,곽지영,이형영 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.6

연구목적 : 본 연구의 목적은 광주광역시의 도시 및 농촌 지역에 거주하는 노인에서 치매의 유병률과 위험인자를 조사하고자 하였다. 부가적으로 이 두 지역간에 치매의 유병률과 위험인자를 비교하고자 하였다. 방 법 : 광주광역시의 전형적인 도시와 농촌지역의 65세 이상의 노인(N=1,598)을 대상으로, 2단계의 역학연구를 수행하였다. 1차 선별 단계에서는 38명의 훈련된 연구보조원이 대상노인을 면담하여, 사회인구학적 특징을 조사하고 한국판 간이정신상태검사(Korean version of Mini-Mental State Examination ; MMSE-K)로 평가하였다. 2차 임상 진단 단계에서는 1차 단계에서 MMSE-K 점수가 24점 이하인 노인(N=431)을 대상으로, 전문연구팀의 임상적 평가를 통해 치매를 진단하고 원인질환별로 알쯔하이머병(Alzheimer's disease ; AD), 혈관성 치매(vascular dementia ; VaD) 및 기타 치매로 분류하였다. 대상노인에서 치매의 유병률을 측정한 후, 광주광역시 전체 노인의 연령보정 유병률을 산출하였다. 전체 대상에서 AD의 위험인자를 조사한 후, 도시와 농촌군으로 나누어 분석을 다시 시행하였다. 결 과 : 1차 및 2차 단계의 참가율은 각각 71.0%(N=1,134) 및 83.2%(N=410) 였다. 연구 참여 노인(N=1,134)에서 전체 치매, AD 및 VaD의 유병률은 최소 각각 9.7%, 5.2%, 1.8%였다. 광주광역시 전체 노인의 연령 보정 유병률은 각각 10.7%, 5.7%, 1.9%인 것으로 산출되었다. AD의 독립 위험인자는 고령, 여성, 무학이었다. 도시와 농촌에 따른 유병률의 차이는 파악되지 않았다. 그러나 AD의 위험인자는 지역에 따라 차이가 있었다. 도시 노인에게는 무학만이, 농촌 노인에게는 고령과 여성인 경우가 독립 위험인자였다. 결 론 : 광주광역시 노인에서 치매의 유병률은 높았고, 이는 국내 다른 지역에서 수행된 선행연구 결과와 유사하였다. 알쯔하이머형 치매의 위험인자는 도시와 농촌에 따라 다른 결과를 보였다. 본 연구 결과는 한국에서의 치매의 실태를 반영하며, 치매의 적절한 관리와 예방을 위해서는 지역성 특성이 고려되어야 함을 시사한다. Objectives : The purpose of this study was to investigate the prevalence rate and risk factors of dementia in an urban and a rural sample of older persons in the metropolitan Kwangju area, Republic of Korea. In addition, the prevalence rate and risk factors of dementia between the two samples were compared. Methods : A two-phase epidemiological study of residents aged 65 or over(N=1,598) was conducted in a highly developed, urban and a non-contiguous, poorly developed, rural area of metropolitan Kwangju. In the first(screening) phase, 38 trained research assistants collectively peformed in-home interviews for all participants. Detailed sociodemographic characteristics were gathered and the Korean version of Mini-Mental State Examination (MMSE-K) was administered. In the second (diagnosing) phase, diagnosis of dementia was established for those scoring 24 or less on the MMSE-K in the screening phase(N=431), and etiological types of dementia were classified to Alzheimer's disease(AD), vascular dementia(VaD) or miscellanous type by two teams of experts. Prevalence rate of dementia was determined in the participants, and age-standardized prevalence rate adjusted by age distribution in metropolitan Kwangju was estimated. For AD, the risk factors were investigated in all participants, and analyses were repeated for the urban and rural samples separately. Results : The participating rates in the first and second phases were 71.0%(N=1,134) and 83.2%(N=410), respectively. In the participants, the prevalence rates of dementia(all types), AD and VaD were 9.7%, 5.2% and 1.8%, respectively. The age-standardized prevalence rates were 10.7%, 5.7% and 1.9%, respectively. Aging, female gender and no education(complete lack of formal education) were identified as independent risk factors for AD. No difference between the urban and rural samples was observed in the prevalence rates of dementia(all types), AD and YaD. However, risk factors for AD differed between these areas. In the urban sample, no education was the only significant factor ; whereas, aging and female gender were identified as risk factors in the rural sample. Conclusion : A high prevalence rate of dementia was observed among old persons in metropolitan Kwangju, consistent with previous studies conducted in other Korean areas. The risk factor profi1es for AD differed between urban and rural settings. The results of this study reflect the current status of dementia in Korea and suggests that appropriate management and prevention strategies for dementia should be tailored toward the geographic settings.

영지(Ganoderma lucidum)의 액체배양에 의한 세포외 다당의 항암활성과 세포증식 및 분화에 미치는 영향

이신영,강태수,문순옥 강원대학교 산업기술연구소 2005 産業技術硏究 Vol.25 No.B

Exopolysaccharide (CBP) from submerged culture broth of Ganoderma lucidum mycelium and the water soluble (BWS) and water insoluble (BWI) fractions of CBP were prepared by gel filtration. Antitumor activity and effects on proliferation and differenciation of human cancer cells and mouse NIH 3T3 cells were studied. Cytotoxicity test of CBP, BWS and BWI fractions on human cancer cell lines was performed by using sulforhodamine B (SRB) assay. A549 (lung carcinoma), Colo320 Dm and HSR (colon carcinoma), and NIH 3T3 cells were used. BWI fraction showed the strongest cytotoxicity (maximum 20% survival) to all human cells tested. However it did not induced apoptosis. Interestingly BWI fraction did not exert cytotoxic effect on NIH 3T3 cells at low concentration of cell (5x10^(4)) but strong toxic effect at high concentration of cell(5x10^(5)) which showed transformed morphology. These results suggest that BWI may have cancer cell specific anticancer activity. However, BWI fraction did not effect the amount of pRb and c-myc protein, which implied that BWI fraction did not act at the early stage of signal transduction pathway. CBP fraction induced differenciation of human leukemic cell line, HL-60 cells suggesting the carcinogenesis prevention of normal cell and possible induction of normalization for cancer cell.

감의 연화 특성

金順東,李信浩,姜明秀 대구효성가톨릭대학교 1986 연구논문집 Vol.33 No.1

To investigate the softening characteristics of persimmonfruit, textural changes at temperature of 25℃, 35℃ and 45℃, and the soaking effect at 30℃, 50℃ and 70℃ were estimated. And also, content and composition of the cell wall polysaccharides, 4NKOH soluble hemicellulosic fraction(HF?), 0~3N KOH soluble hemicellulosic fraction(HF?), ionically associated pectin(IAP), covalently bounded pectin(CBP) and cellulosic fraction(CF), and polygalacturonase(PG), cell wall polysaccharides degradated crude enzyme(CEW) were studied. The hardness was lowest at 25℃, especially adhesiveness and gumminess was contrary to each other in the temperature. In the higher temperature, 35~45℃, the softening phenomenon was inhibited by degrees, but adhesiveness and gumminess showed high on the 6th days at 35℃. The softening was more promoted by soaking at 30℃, and showed higher adhesiveness. In the higher soaking temperature of 50℃ to 70℃, the adhesiveness and the gumminess was increased, but the softening was getting inhibited. The persimmon contained lower pectic polysaccharides, but PG activity was high(720μmol product/100g f.w.). IAP and CBP was degradated about 60% by the CWE, and the degradation ratio of the pectic polysaccharides was uronic acid 43~50%, hexose 59~61%. It was suggested that the severe softening phenomenon of persimmon involoved the degradation of CBP-bound hemicellulosic fraction.

영지(Ganoderma lucidum)균사체의 액체배양에 의한 세포외 수용성 다당류의 분획 및 항암활성

이신영,강태수,문순옥,류인덕,이명열 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

영지 균사체의 액체배양에 의하여 생산된 세포외 다당 시료를 분획하였고, 이들 각 분획에 대한 항암 활성을 균사체 유래 다당 분획들과 비교하면서 조사하였다. 배양여액으로부터 세포외 수용성 다당(BWS)을 얻었고, 이를 순차적으로 이온교환 및 겔 크로마토그라피 하여 BWS를 포함한 세포의 수용성 다당 분획, 총 5종을 얻었으며, 균사체 유래의 수용성 다당(MWS)에서도 세포외 다당과 동일한 방법으로 분획하여 총 5종의 분획을 얻어 대조구로 시용하였다. 세포외 다당 시료들의 항보체 활성(ITCH_50, %)은 15~30% 정도로 15~70%인 균사체 유래 다당 분획들의 값보다는 낮았으나, 이들 세포외 다당 분획들의 값보다는 낮았으나, 이들 세포외 다당 분획들중, BWS-DA와 BWS-DA-GI는 1000 ㎍/ml의 시료 농도에서 30% 정도의 매우 높은 활성을 나타내었다. MTT assay에서는 mouse leukemia L1210에 대하여 BWS-DN 및 MWS가 600㎍/ml의 농도에서 각각 86 및 89%의 높은 저해율을 나타내었으며, BWS-DA 및 MWS-DN의 경우는 3㎍/ml의 매우 낮은 농도에서도 32~35%의 저해율을 보였다. 또 human cell line인 COLO-205에 대해서는 BWS와 BWS-DA 및 MWS-DA가, 그리고 HL-60에 대해서는 BWS-DA와 BWS-DN 및 MWS-DN이 각각 300㎍/ml의 시료 농도에서 약 50%(IC_50)의 높은 저해능을 나타내었다. Exo-polysaccharide (BWS) obtained from submerged cultivation of Ganoderma lucidum mycelium was fractionated. Antitumor activity of their fractions was investigated in comparison with the mycelial polysaccharide fractions. Eight kinds (BWS-DN, BWS-DA, BWS-DN-GI, BWS-DA-GI, MWS-DN, MWS-DA, MWS-DN-GI and MWS-DA-GI) of polysaccharide fractions were obtained by DEAE-cellulose ion exchange chromatography and Sepharose CL-4B gel chromatography from BWS and MWS, which were isolated from culture fluid and mycelial cell, respectively. The anticomplementary activities (ITCH_50, %) of the exo-polysaccharide fractions showing 15% to 30% were lower than those of mycelial polysaccharide fractions showing 15% to 70%. The acidic fractions of BWS-DA and BWS-DA-GI fractionated from BWS, showed the highest activity of 30%. In the MTT assay, BWS-DN and MWS against mouse leukenia L1210 exhibited high inhibition ratio of 86 and 89%, respectively at the concentration of 600 ㎍/ml. High inhibition ratio of 50% (IC_50) was achieved for BWS, BWS-DA and MWS-DA fractions against human colon adenocarcinoma COLO-205 and for BWS-DN, and MWS-DN fractions against human leukemia HL-60 at the concentration of 300 ㎍/ml among the six polysaccharide fractions, respectively.

편측안면경련에 대한 미세혈관압술시 수술시야 확보를 위한 방법으로서 소뇌피질동맥 박리술 - 수 술 수 기 -

김범태,임수빈,장재칠,신윈한,최순관,변박장 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.11

Rocuronium의 애별투여방법이 모지내전근의 이완에 미치는 영향

최영균,김승수,이원진,조광래,이상은,김영환,임세훈,이정한,이근무,정순호,김영재,신치만 인제대학교 2009 仁濟醫學 Vol.30 No.-

Objective : There are controversies in pnmmg technique of rocuronium whether it can or can't shorten the onset time. We want to compare the effects of two different priming doses and intervals of rocuronium. Materials and Methods : After giving propofol, 50 patients were randomly assigned to 5 groups. Group I received a placebo, followed 3 min later by rocuronium 0.6 mg/kg. Priming doses and intervals of Group II, Ill, N, V were 0.06 rng/kg and 2 min, 0.12 mg/kg and 2 min, 0.06 rug/kg and 3 min, 0.12 mg/kg and 3 min, respectively. Total dose of rocuronium was 0.6mg/kg. Train of four (TOF) count or ratio and 1st twitch height of TOF (T1) were monitored with 15 secondsintervals. TOF ratio of each groups just before bolus injection, duration for TOF count to reach zero and duration for Tl to reach 95% depression after bolus injection were compared. Results : Duration for TOF count to reach zero and duration for Tl to reach 95% depression after bolus injection were decreased in priming groups compared to Group 1 (P < 0.01) In group V, significant depression of TOF ratio just before bolus injection was seen (P < 0.05). Conclusion : Priming technique with rocuronium is effective method to shorten the onset of neuromuscular block. But priming dose of rocuronium itself could cause hypoxia and aspiration in awake patients and should be used carefully. Priming dose of rocuronium 0.06 mg/kg and 2 minutes interval maybe relatively safe method.

삼차신경통으로 발현된 소뇌교각부의 표피양낭종 : 2례 증례보고

심재준,김범태,임수빈,신원한,최순관,변박장 대한신경외과학회 1997 Journal of Korean neurosurgical society Vol.26 No.8

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2