Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

혈관질환 정보관리 시스템

김동익,김덕경,허세호,이병붕,김용신,김은숙,문지영,도영수,신성욱,김동수,김만태,진재욱,김용신 대한혈관외과학회 2002 Vascular Specialist International Vol.18 No.1

As medical technology progresses rapidly, there is a rise in the average age along with the Korean dietary lifestyle becoming more westernized, which leads to an increase in the number of vascular disease patients in Korea. Thus, we need to manage the medical information of a disease systematically in order to diagnose and treat constructively. However, since there has been no standardized method of man agement to date, a great deal of information could not be properly utilized nor studied. Therefore, the departments of Cardiology, Radiology and Neurology of Samsung Seoul Hospital recently got together to develop an information management system called the Vascular Data System. This program was developed to be run on win98 O/S, upper Pentium Ⅲ, and upper 128 MB Memory, and its source code is Dephi 4.0. It was configured for the user to set the configurations as well as do a variety of search and analysis. If this program were to be updated continuously, it may be used extensively as well as in various parts of clinical research activities.

동의보감 탕액편에 기재된 식이본초의 독성유무에 대한 분류 연구

신호동(Ho-Dong Shin),정종운(Jong-Un Jeong) 대한한의학회 2011 대한한의학회지 Vol.32 No.1

만성 견비통에 대한 동씨침 치료의 무작위 대조군의 임상 연구

김찬영,권나현,신예지,남동우,김건형,김종인,최도영,이윤호,이재동 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : To observe the effect of acupuncture treatment in chronic shoulder pain patients. Methods : 36 voluntary patients were randomly assigned to an acupuncture treatment group(E GrouP, n=18) and a control group(C GrouP, n=18). The E Group patients received acupuncture treatment on LI_(15), TE_(14), GB_(21) and Master Dong's acupuncture points, Shin-gwan and Gyun-joong, twice a week for four weeks. The C Group patients received no treatment. All patients in both groups were instructed to practise self exercise in their daily lives. Evaluations were made at baseline and after four weeks of study. The Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI) and the patient's subjective pain was measured by Visual Analogue Scale(VAS). The obtained data was analyzed. Results '. The E Group showed significant(p<0.05) improvement in CSA, SPADI and VAS after four weeks of treatment. The C Group showed significant(p<0.05) improvement in CSA, but the change of SPADI and VAS was insignificant(P>0.05). CSA and SPADI of E Group significantly(p<0.05) improved compared to the C GrouP, but the difference of VAS change in the two groups was insignificant(p>0.05). Conclusions : Four weeks of acupuncture treatment significantly improved CSA, SPADI and VAS. The improvement of CSA and SPADI was significant(p<0.05) compared to untreated patients.

전신경련을 보인 중증 열성 혈소판 감소 증후군에 의한 뇌병증 1 례: 증례보고

전호영, 이혜림, 여민주, 김지선, 신동익, 이성현, 이상수 충북대학교 의학연구소 2016 忠北醫大學術誌 Vol.26 No.2

본문참고

하수 방류수 살균소독을 위한 무전극 UV Lamp의 개발

신동호,이규현,이용택 한국공업화학회 2003 응용화학 Vol.7 No.2

하수방류수 살균소독에 이용되는 UV 램프를 기존의 전극용보다 효율을 높이고 수명이 긴 무전극 UV 램프를 제작하고 그 성능을 알아보았다. 우선 활성물질을 변화시키면서 제조한 램프의 UV강도 및 온도의 변화를 살펴보았다. 그 결과 활성물질이 Hg/Ind의 무게비 95/5로 만든 램프를 250분간 운전한 결과 UV 강도 300 mW/㎤ 및 표면온도 200-250 ℃로 가장 안정적인 결과를 가져왔다. 그러나 무전극 램프를 장시간 발광시켰을 경우 램프의 표면온도가 상승하기 때문에 실제 하수처리공정에 사용하기 위하여 냉각이 가능한 이중관 형태로 제작하여 UV강도와 온도 변화특성도 알아보았다. 또한 제작된 무전극 UV lamp를 하수 방류수의 살균소독을 위하여 대장균(E-coli.)으로 실험한 결과 99.9% 이상의 살균효율을 보였다.

두부손상 환자의 정신과적 합병증에 관한 장애 감정례의 일반 사항에 관한 분석

신석철,왕성근,신윤오,김덕호,김상국,강동숙 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

Authorst ried retrospective analysis to find out general characteristics of the patients undergoing disability evaluation at the department of psychiatry Chungnam National University Hospital from 1986 to 1990. The subjects, who were suffered from psychiatric complications due to head trauma by traffic accident, were 66 patients and they were classified depending on the demographic status, situations of traffic accident, evalaution periods, whether or not perform operation, and rates of evaluated disability and diagnosis. The results were summarized as follows. 1. General background of the subjects were as follows. 1) The frequency order of request were court(59 %) and insurance company(19.7%). 2) The place of accident was more common in large city, common accident vehicle were bus, truck, and cabs. 3) The season of accident were more common in Spring and Autumn. 2. Male(71.2%) was more common than in female and more common in the age group of 5-19 years old(37.9%). The most frequent job of the patients was labour, and the education level was more common in the graduates of elementary school(47.9% ). 3. The interval between accident and evaluation was most frequent in 12-23 months(47.5%), and duration of evaluation was mostly 14 to 21 days. Performed brain operation was 31.8% and non-operated patients was 50.0%. The rates of disability according to the McBride's disability evaluation were mostly belonged to the group of beow 40%(74.1%). 4. The final diagnosis after evaluation were organic personality disorder(45.5%) and dementia(31.8%).

Prader-Willi 증후군의 Fluoxetine 치험 1례

신동원,송동호 대한소아청소년정신의학회 1997 소아청소년정신의학 Vol.8 No.1

지역복지 정보네트워크의 구축에 관한 연구

신동면,한상연,김종호,이호창,김민용 한국정책학회 2004 韓國政策學會報 Vol.13 No.1

이 논문에서는 정보기술이 지역복지 전달체계의 개편에 기여할 수 있다는 인식을 바탕으로 지역복지 정보네트워크를 구축하기 위한 모델과 지역복지 정보네트워크를 위한 컨텐츠 구성 방안 등에 대하여 논의하고 있다. 정보의 공유와 상호협력 관계를 충실하게 구혈할 수 있는 정보네트워크 구축방식은 지역복지 전달과정에 관련된 공급자, 수혜자, 후원자, 그리고 정부를 포함한 모든 당사자들을 상호 연결하는 e-허브 모델이 바람직하다. 지역복지 네트워크 허브는 지역복지 전달과정에서 각 주체들이 정보네트워크에 올린 원시정보를 분류?축적?갱신함으로써 사회복지서비스의 수급을 연결시키는 역할을 담당하기 때문에 지방자치단체가 운영의 책임을 져야 한다. 지역복지 정보네트워크는 지역복지 전달과정에서 5C 활동-컨텐츠(Contents), 의사소통(Communication), 커뮤너티(Community), 협업(Collaboration), 기여(Contribution) -을 촉진할 것이다. 이중에서 특히 컨텐츠의 수준은 지역복지 정보네트워크가 효과적으로 운영되기 위한 관건이 된다. 이에 본 논문에서는 공급자, 수혜자, 후원자, 그리고 정부가 지역복지 정보네트워크의 운영을 위하여 제공해야 할 컨텐츠를 구체적으로 예시하고 있다. 지역복지 정보네트워크는 5C 활동을 통하여 지역복지 전달과정에서 관련 당사자들 간의 상호, 협력적 관계를 지속시킴으로써 지역복지 전달체계의 효율성, 적절성, 통합성, 전문성, 접근성을 제고하는 데 기여할 것이다.