http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Shahi, Pawan Kumar (검색결과 87 건)
- 무료
- 기관 내 무료
- 유료
-
-
Shahi, Pawan Kumar,Choi, Seok,Zuo, Dong Chuan,Yeum, Cheol-Ho,Yoon, Pyung-Jin,Lee, Jun,Kim, Young-Dae,Park, Chan-Guk,Kim, Man-Yoo,Shin, Hye-Rang,Oh, Hyun-Jung,Jun, Jae-Yeoul The Korean Society of Pharmacology 2011 The Korean Journal of Physiology & Pharmacology Vol.15 No.3
In this study we determined whether or not 5-hydroxytryptamine (5-HT) has an effect on the pacemaker activities of interstitial cells of Cajal (ICC) from the mouse small intestine. The actions of 5-HT on pacemaker activities were investigated using a whole-cell patch-clamp technique, intracellular $Ca^{2+}$ ($[Ca^{2+}]_i$) analysis, and RT-PCR in ICC. Exogenously-treated 5-HT showed tonic inward currents on pacemaker currents in ICC under the voltage-clamp mode in a dose-dependent manner. Based on RT-PCR results, we found the existence of 5-$HT_{2B,\;3,\;4,\;and\;7}$ receptors in ICC. However, SDZ 205557 (a 5-$HT_4$ receptor antagonist), SB 269970 (a 5-$HT_7$ receptor antagonist), 3-tropanylindole - 3 - carboxylate methiodide (3-TCM; a 5-$HT_3$ antagonist) blocked the 5-HT-induced action on pacemaker activity, but not SB 204741 (a 5-$HT_{2B}$ receptor antagonist). Based on $[Ca^{2+}]_i$ analysis, we found that 5-HT increased the intensity of $[Ca^{2+}]_i$. The treatment of PD 98059 or JNK II inhibitor blocked the 5-HT-induced action on pacemaker activity of ICC, but not SB 203580. In summary, these results suggest that 5-HT can modulate pacemaker activity through 5-$HT_{3,\;4,\;and\;7}$ receptors via $[Ca^{2+}]_i$ mobilization and regulation of mitogen-activated protein kinases.
-
Shahi, Pawan Kumar,Choi, Seok,Jeong, Yu Jin,Park, Chan Guk,So, Insuk,Jun, Jae Yeoul Springer-Verlag 2014 Naunyn-Schmiedeberg's archives of pharmacology Vol.387 No.7
<P>Cyclic guanosine 3',5'-monophosphate (cGMP) inhibited the generation of pacemaker activity in interstitial cells of Cajal (ICCs) from the small intestine. However, cGMP role on pacemaker activity in colonic ICCs has not been reported yet. Thus, we investigated the role of cGMP in pacemaker activity regulation by colonic ICCs. We performed a whole-cell patch-clamp and Ca(2+) imaging in cultured ICCs from mouse colon. 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, an inhibitor of guanylate cyclase) increased the pacemaker potential frequency, whereas zaprinast (an inhibitor of phosphodiesterase) and cell-permeable 8-bromo-cGMP decreased the pacemaker potential frequency. KT-5823 (an inhibitor of protein kinase G [PKG]) did not affect the pacemaker potential. L-N(G)-nitroarginine methyl ester (L-NAME, an inhibitor of nitric oxide [NO] synthase) increased the pacemaker potential frequency, whereas ()-S-nitroso-N-acetylpenicillamine (SNAP, a NO donor) decreased the pacemaker potential frequency. Glibenclamide (an ATP-sensitive K(+) channel blocker) did not block the effects of cell-permeable 8-bromo-cGMP and SNAP. Recordings of spontaneous intracellular Ca(2+) ([Ca(2+)]i) oscillations revealed that ODQ and L-NAME increased [Ca(2+)]i oscillations. In contrast, zaprinast, 8-bromo cGMP, and SNAP decreased the [Ca(2+)]i oscillations. Basal cGMP levels regulate the resting pacemaker potential frequency by the alteration on Ca(2+) release via a PKG-independent pathway. Additionally, the endogenous release of NO seems to be responsible maintaining basal cGMP levels in colonic ICCs.</P>
-
Mismatch-repair protein MSH6 is associated with Ku70 and regulates DNA double-strand break repair
Shahi, Ankita,Lee, Jung-Hee,Kang, Yoonsung,Lee, Sung Haeng,Hyun, Jin-Won,Chang, In-Youb,Jun, Jae-Yeoul,You, Ho Jin Oxford University Press 2011 Nucleic acids research Vol.39 No.6
<P>MSH6, a key component of the MSH2–MSH6 complex, plays a fundamental role in the repair of mismatched DNA bases. Herein, we report that MSH6 is a novel Ku70-interacting protein identified by yeast two-hybrid screening. Ku70 and Ku86 are two key regulatory subunits of the DNA-dependent protein kinase, which plays an essential role in repair of DNA double-strand breaks (DSBs) through the non-homologous end-joining (NEHJ) pathway. We found that association of Ku70 with MSH6 is enhanced in response to treatment with the radiomimetic drug neocarzinostatin (NCS) or ionizing radiation (IR), a potent inducer of DSBs. Furthermore, MSH6 exhibited diffuse nuclear staining in the majority of untreated cells and forms discrete nuclear foci after NCS or IR treatment. MSH6 colocalizes with γ-H2AX at sites of DNA damage after NCS or IR treatment. Cells depleted of MSH6 accumulate high levels of persistent DSBs, as detected by formation of γ-H2AX foci and by the comet assay. Moreover, MSH6-deficient cells were also shown to exhibit impaired NHEJ, which could be rescued by MSH6 overexpression. MSH6-deficient cells were hypersensitive to NCS- or IR-induced cell death, as revealed by a clonogenic cell-survival assay. These results suggest a potential role for MSH6 in DSB repair through upregulation of NHEJ by association with Ku70.</P>
-
Shahi, Pawan Kumar,Choi, Seok,Zuo, Dong Chuan,Kim, Man Yoo,Park, Chan Guk,Kim, Young Dae,Lee, Jun,Park, Kyu Joo,So, Insuk,Jun, Jae Yeoul Springer Japan 2014 Journal of gastroenterology Vol.49 No.6
<P><B>Background</B></P><P>Hyperpolarization-activated cyclic nucleotide (HCN) channels are pacemaker channels that regulate heart rate and neuronal rhythm in spontaneously active cardiac and neuronal cells. Interstitial cells of Cajal (ICCs) are also spontaneously active pacemaker cells in the gastrointestinal tract. Here, we investigated the existence of HCN channel and its role on pacemaker activity in colonic ICCs.</P><P><B>Methods</B></P><P>We performed whole-cell patch clamp, RT-PCR, and Ca<SUP>2+</SUP>-imaging in cultured ICCs from mouse mid colon.</P><P><B>Results</B></P><P>SQ-22536 and dideoxyadenosine (adenylate cyclase inhibitors) decreased the frequency of pacemaker potentials, whereas both rolipram (cAMP-specific phosphodiesterase inhibitor) and cell-permeable 8-bromo-cAMP increased the frequency of pacemaker potentials. CsCl, ZD7288, zatebradine, clonidine (HCN channel blockers), and genistein (a tyrosine kinase inhibitor) suppressed the pacemaker activity. RT-PCR revealed expression of HCN1 and HCN3 channels in c-kit and Ano1 positive colonic ICCs. In recordings of spontaneous intracellular Ca<SUP>2+</SUP> [Ca<SUP>2+</SUP>]<SUB>i</SUB> oscillations, rolipram and 8-bromo-cAMP increased [Ca<SUP>2+</SUP>]<SUB>i</SUB> oscillations, whereas SQ-22536, CsCl, ZD7288, and genistein decreased [Ca<SUP>2+</SUP>]<SUB>i</SUB> oscillations.</P><P><B>Conclusions</B></P><P>HCN channels in colonic ICCs are tonically activated by basal cAMP production and participate in regulation of pacemaking activity.</P>
-
Shahi Pratyush,Chadha Manish,Sehgal Apoorv,Sudan Aarushi,Meena Umesh,Bansal Kuldeep,Batheja Dheeraj 대한척추외과학회 2022 Asian Spine Journal Vol.16 No.3
Study Design: Cross-sectional study.Purpose: To evaluate sagittal balance, pulmonary function, and spinopelvic parameters in patients with healed spinal tuberculosis with severe thoracic kyphosis.Overview of Literature: Deterioration of neurological function is an absolute indication of surgical intervention in severe post-tubercular kyphosis, but the relationship of compromise in lung function and spinal alignment with severity of kyphosis is still unclear.Methods: Twenty patients (age, 14–60 years) with healed spinal tuberculosis with thoracic kyphosis >50° were included. Lateral-view radiography of the whole spine, including both hips, was performed for assessment of kyphotic angle (K angle), sagittal balance, lumbar lordosis, and spinopelvic parameters. Pulmonary function was assessed by measuring the forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), and their ratio (FEV1/FVC) by spirometry.Results: A positive correlation between severity of kyphosis and sagittal imbalance was noted, with compensatory mechanisms maintaining the sagittal balance in only up to 80° of dorsal kyphosis. In >80° of kyphosis, FVC was found to be markedly decreased (mean FVC=50.6%). The mean K angle was lower in subjects with lower thoracic kyphosis. In lower thoracic kyphosis, due to short lordotic and long kyphotic curves, both lumbar lordosis and pelvic retroversion worked at compensation, whereas, in middle thoracic kyphosis, due to long lordotic curve, only lumbar lordosis was required. Normal pulmonary function (mean FVC, 83.0%) and lesser kyphotic deformity (mean K angle in adolescents, 69.8°; in adults, 94.4°) were found in adolescents.Conclusions: In >80° of thoracic kyphosis, there is sagittal imbalance and a markedly affected pulmonary function. Such patients should be offered corrective surgery if they are symptomatic and medically fit to undergo the procedure. However, whether the surgical procedure would result in improved pulmonary function and sagittal balance needs to be evaluated by a follow-up study.
-
-
-
Assessment of Morphological Variation between Some Iranian Wild Cerasus Sub-genus Genotypes
Ali Shahi-Gharahlar,Zabihollah Zamani,Mohammad Reza Fatahi,Naser Bouzari 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.4
Iran is one of the important countries for the distribution of Cerasus subgenus species with around 225,000 tons production of cherries. Together with Turkey and USA it has been one among the three leading producers of these fruits. One of the important aims in cherry breeding is developing dwarfing rootstocks with good adaptation with different climates and resistance to biotic and abiotic stresses. A program has been initialized in Iran for breeding and developing new rootstocks for cherries, suitable for climatic and edaphic condition of this country. The present study was conducted under this program to categorize some Cerasus sub-genus wild genotypes and improved cultivars and rootstocks, by using vegetative characters. Conservation of the genetic resources of these species is another important aim of this program. Around 27 morphological parameters were evaluated on 74 genotypes in seven species of this sub-genus from eight provinces of Iran. These consisted of P. avium L., P. cerasus L., P. mahaleb L., P. incana Pall., P. brachypetala Boiss., P. pseudoprostrata Pojark. and P. microcarpa Bioss. Analysis of variance of the studied parameters was significant among genotypes, showing a high level of variability for the characters. Results of simple correlation analysis showed the existence of significant positive and negative correlations among some important parameters such as tree height, tree growth habit, trunk diameter, internode length, growth compactness and leaf area. Also, factor analysis showed that tree growth habit, height, growth compactness, trunk diameter, internode length, pubescence on the leaf upper surface and number of leaf serration per cm constitute the main factors. Effective characters were categorized within six factors and accounted for 77.74 of overall variance. Cluster analysis was performed using these six factors and genotypes in the approximate distance of 13 were divided into six main groups. These groups were divided into several sub-clusters and improved varieties or cultivars were clearly separated from the others. The P. cerasus cultivars and genotypes together with one genotype of the P. avium and improved rootstocks (‘Gisela6’ and ‘Colt’) located in one sub-cluster. Scatter plot analysis using two main factors could also clearly separate the location of genotypes and verified genotypes from each other.
-
Floor-Mounted Robotic Pedicle Screw Placement in Lumbar Spine Surgery: An Analysis of 1,050 Screws
Pratyush Shahi,Omri Maayan,Daniel Shinn,Sidhant Dalal,Junho Song,Kasra Araghi,Dimitra Melissaridou,Avani Vaishnav,Karim Shaf,Yuri Pompeu,Evan Sheha,James Dowdell,Sravisht Iyer,Sheeraz A. Qureshi 대한척추신경외과학회 2023 Neurospine Vol.20 No.2
Objective: To analyze the usage of floor-mounted robot in minimally invasive lumbar fusion. Methods: Patients who underwent minimally invasive lumbar fusion for degenerative pathology using floor-mounted robot (ExcelsiusGPS) were included. Pedicle screw accuracy, proximal level violation rate, pedicle screw size, screw-related complications, and robot abandonment rate were analyzed. Results: Two hundred twenty-nine patients were included. Most surgeries were primary single-level fusion. Sixty-five percent of surgeries had intraoperative computed tomography (CT) workflow, 35% had preoperative CT workflow. Sixty-six percent were transforaminal lumbar interbody fusion, 16% were lateral, 8% were anterior, and 10% were a combined approach. A total of 1,050 screws were placed with robotic assistance (85% in prone position, 15% in lateral position). Postoperative CT scan was available for 80 patients (419 screws). Overall pedicle screw accuracy rate was 96.4% (prone, 96.7%; lateral, 94.2%; primary, 96.7%; revision, 95.3%). Overall poor screw placement rate was 2.8% (prone, 2.7%; lateral, 3.8%; primary, 2.7%; revision, 3.5%). Overall proximal facet and endplate violation rates were 0.4% and 0.9%. Average diameter and length of pedicle screws were 7.1 mm and 47.7 mm. Screw revision had to be done for 1 screw (0.1%). Use of the robot had to be aborted in 2 cases (0.8%). Conclusion: Usage of floor-mounted robotics for the placement of lumbar pedicle screws leads to excellent accuracy, large screw size, and negligible screw-related complications. It does so for screw placement in prone/lateral position and primary/revision surgery alike with negligible robot abandonment rates.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
