고콜레스테롤혈증 환자에서 Atorvastation의 유용성 및 안전성에 관한 연구

고종훈,신준한,김한수,탁승제,최병일,김동수,권혁문,김현승 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.9

소비-노동의 환경파괴적 순환구조와 놀이

고현욱,김승모 慶南大學校 産業經營硏究所 1997 産業經營 Vol.21 No.-

쌀가루와 감자를 농후제로 사용한 크림스프의 품질 특성

고승정,박홍현,이경희 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.6

In order to seek the possibility of using rice flour and potato as thickening agent instead of roux, sensory evaluations of cream soups manufactured from 3 different thickening agents were conducted. The viscosity, moisture and lipid contents, and the color affecting the quality of the soup were investigated. The results were as follows: 1. As thickening agents for 200g of the cream soups, rice flour and potatoes at 20.5 and 90g, respectively, were needed in order to make soup with a similar concentration to that manufactured using 20g of roux. 2. In the sensory tests, the soup made with rice flour had the most preferred overall acceptability, with a significantly preferable taste. The soup made with rice flour had the strongest whiteness and savory taste, but that made with roux had the strongest viscosity and smooth feeling in the mouth feel. There was no difference between the samples with regard to nutty flavor. The whiteness, palatability, viscosity and smooth feel sensory properties were positively correlated with the overall acceptability. Among these properties, the palatability showed the highest correlation (r=0.538) with overall preference. 3. The cream soup made using potatoes as the thickening agent had a 76.20% water content, whereas those made with rice flour and roux had 73.00 and 64.00% respectively. The fat content of the cream soup made with roux was 10.24%, which was twice as high as those of the rice flour and potato soups. 4. Among the 3 kinds of cream soup, the one made with rice flour had the whitest color

일개 소방서 소방관들의 외상후 스트레스 증상 및 관련요인

권순찬,송재철,이수진,김인아,고재우,류현철,김석현,김대호,정승아 大韓産業醫學會 2008 대한직업환경의학회지 Vol.20 No.3

목적: 본 연구에서는 일부지역 남자 소방관의 외상후 스트레스 증상과 관련요인을 분석하고 PTSD 고위험군의 빈도를 추정하고자 하였다. 방법: 서울 지역 한 소방서의 남자 소방대원 260명에게 설문지를 배포하였다. 그 중 한 가지 이상의 외상성 사건의 경험이 있고 IES-R-K 설문에 응답한 153명 중 우울증으로 의심되는 7명을 제외한 146명을 분석대상으로 하였다. 설문지는 일반적 특성과 작업관련 특성, IES-R-K 22문항,‘소방관과 응급구조사의 사건 스트레스인자’25개 문항을 포함하였다. 결과: 연구대상의 평균연령은 38.8(±6.8)세였으며 평균 근속기간은 12.0(±6.4)년이었다. 기혼이 118명 (80.8%)이었다. 근무형태별로는 상시주간이 20명(13.7%),격일근무가 126명(86.3%)이었다. 직능별로는 화재진압 46명(31.5%),구조 15명(10.3%),구급 26명(17.8%),운전 40명(27.4%),내근 19명(13.0%)이었다. IES-R-K 점수의 평균은 11. 3(±13.1)점이었으며 기혼자가 미혼자보다(p=0.005),24시간 격일근무자가 상시 주간근무자보다(p=0.032) 높았다. IES-R-K 점수 24/25점을 절단점으로 PTSD 고위험군의 빈도는 13.7% (20명)이었다. 외상성 사건 25개 중 경험한 종류수는 직능별로 구급과 구조에서 많았다.(p=0.000). PTSD 고위험군을 종속변수로 하고 나이와 교육수준을 보정한 다중로지스틱회귀분석에서 각각의 보정 비차비(95% CI)는 기혼 1.98(0.42∼9.30),근속년수 20년 이상 3.53(1.01∼12.38),24시간 격일근무 3. 31 (O.42∼26. 23),소방장 1.67(0.46∼6.09),외상성 사건을 경험한 종류수 2.18(0.79∼6.04)였다. 결론: 이 연구에서 서울지역의 일개 소방서에 근무하는 남자 소방관의 PTSD 고위험군의 빈도는 13.7%로 추정되어 일반인구집단의 PTSD 유병률에 비해 높은 것으로 나타났다. 외상성 사건에 무방비로 노출되어 있는 소방관들의 PTSD 위험군을 조기에 선별할 수 있는 간편한 자기기입식 설문지인 IES-R-K는 소방관의 PTSD를 조기에 개입하여 관리하는 효율적인 도구로 활용될 수 있을 것이다. 소방관들의 정신건강을 관리하고 개입이 필요한 정신질환을 조기에 발견하며 관리할 수 있는 체계적인 예방 및 관리 프로그램의 도입이 필수적이다. Objectives: To investigate the factors related with posttraumatic symptoms and to estimate the prevalence of posttraumatic disorder of urban male firefighters. Methods: From a study sample comprising 260 male firefighters from one fire station, the responses. from 146 who had experienced one or more traumatic events, responded to the 'Impact of Event Scale-Revised Korean version' (IES-R-K) and didn't show any evidence of depression were analyzed. We surveyed the firefighters using a self-administered questionnaire including general characteristics, job-related characteristics, IES-R-K, and a 'Firefighters and paramedics' incident stressor'. Results: The mean value of IES-R-K in this study was 11.3 and it was higher in the married firefighters than in the unmarried firefighters (p=0.005) and in the 24-hr shift workers than in the ordinary day shift workers (p=0.032). The frequency of posttraumatic stress disorder (PTSD) of the firefighters in this study using IES-R-K was 13.7%, with a cutoff point of 24/25. The overall number of experiences of 25 traumatic events was larger in emergency medical personnel and rescue workers than in other work functions (p=O.OOO). Conclusions: The prevalence of PTSD symptoms in firefighters was estimated to 13.7% using IES-R-K and was higher than the prevalence of PTSD in the general population. A program to manage the mental health and provide early detection for mental illness for firefighters is needed.

Session C : Cell Biology : Transport , Growth , and Differentiation : The induction of transgenic tobacco transformed by v - H - ras gene

Hyun Seung Koh,Jee Sun Noh,Jung Hee Kyun,Mi Rim Kim,Kyu Chung Hur 한국생화학분자생물학회 (구 한국생화학회) 1993 추계학술대회집 Vol.- No.-

Solubilities of Salen Derivatives and Their Cobalt Complex in Liquid and Supercritical CO₂

Koh, Seung-Hyun,Jeon, Byung-Wan,Kim, Ha-Kwon,Park, Kwang-Heon,Kim, Hong-Doo Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.4

The solubility of N,N'-Bis(salicylidene) ethylenediamine (n-salen) and N,N'-bis(3,5-di-tert-butylsalicylidene) ethylenediamine (t-butyl-salen) was studied with in-situ UV-VIS spectrometer. n-Salen is 3-5 times more soluble than t-butyl-salen in liquid or supercritical $CO_2$. This behavior may be attributed to Lewis acid-base interaction between salen and $CO_2$. The chelation of salen with $Co^{++}$ ion in supercritical condition was confirmed to be fast enough above room temperature. However, the metal ion extraction capability of t-butyl salen is relatively poor because of its low solubility and ionic nature of complex.

Cellular direct conversion by cell penetrable OCT4-30Kc19 protein and BMP4 growth factor

Seung Hyun L. Kim,Sungwoo Cho,Seoyeon Kim,권정후,Jaeyoung Lee,Rachel H. Koh,Ju Hyun Park,이화진,Tai Hyun Park,Nathaniel S. Hwang 한국생체재료학회 2022 생체재료학회지 Vol.26 No.3

Background: The number of patients suffering from osteoporosis is increasing as the elderly population increases. The demand for investigating bone regeneration strategies naturally arises. One of the approaches to induce bone regeneration is somatic cell transdifferentiation. Among the transcriptional regulators for transdifferentiation, octamer-binding transcription factor 4 (OCT4) is famous for its role in the regulation of pluripotency of stem cells. Bone morphogenetic protein 4 (BMP4) is another factor that is known to have a significant role in osteogenic differentiation. Previous studies have achieved transdifferentiation of cells into osteoblasts using viral and plasmid deliveries of these factors. Although these methods are efficient, viral and plasmid transfection have safety issues such as permanent gene incorporations and bacterial DNA insertions. Herein, we developed a cell penetrating protein-based strategy to induce transdifferentiation of endothelial cells into osteoblasts via nuclear delivery of OCT4 recombinant protein combined with the BMP4 treatment. For the nuclear delivery of OCT4 protein, we fused the protein with 30Kc19, a cell-penetrating and protein stabilizing protein derived from a silkworm hemolymph of Bombyx mori with low cytotoxic properties. This study proposes a promising cell-based therapy without any safety issues that existing transdifferentiation approaches had. Methods: OCT4-30Kc19 protein with high penetrating activities and stability was synthesized for a protein-based osteogenic transdifferentiation system. Cells were treated with OCT4-30Kc19 and BMP4 to evaluate their cellular penetrating activity, cytotoxicity, osteogenic and angiogenic potentials in vitro. The osteogenic potential of 3D cell spheroids was also analyzed. In addition, in vivo cell delivery into subcutaneous tissue and cranial defect model was performed. Results: OCT4-30Kc19 protein was produced in a soluble and stable form. OCT4-30Kc19 efficiently penetrated cells and were localized in intracellular compartments and the nucleus. Cells delivered with OCT4-30Kc19 protein combined with BMP4 showed increased osteogenesis, both in 2D and 3D culture, and showed increased angiogenesis capacity in vitro. Results from in vivo subcutaneous tissue delivery of cell-seeded scaffolds confirmed enhanced osteogenic properties of transdifferentiated HUVECs via treatment with both OCT4-30Kc19 and BMP4. In addition, in vivo mouse cranial defect experiment demonstrated successful bone regeneration of HUVECs pretreated with both OCT4-30Kc19 and BMP4. Conclusions: Using a protein-based transdifferentiation method allows an alternative approach without utilizing any genetic modification strategies, thus providing a possibility for safer use of cell-based therapies in clinical applications.

췌장의 관내 유두상 점액성 종양의 임상적 고찰

조승현(Seung Hyun Cho),허영회(Young Hoe Hur),고양석(Yang Seok Koh),김정철(Jung Chul Kim),조철균(Chul Kyoon Cho),김현종(Hyun Jong Kim) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.6

Purpose: Intraductal papillary mucinous tumor (IPMT) of the pancreas pathologically shows papillary proliferation and its tumor cells display a spectrum of changes ranging from adenoma to infiltrating carcinoma. Because of this variability, there have been many difficulties for making an accurate diagnosis and administering proper treatment. The aim of this study was to determine the treatment strategy and differential diagnosis of benign and malignant IPMT. Methods: Between January 2000 and June 2007, 24 patients with IPMT of the pancreas underwent surgery. The relationships among the clinopathologic features and tumor locations and subtypes were retrospectively investigated. In addition, the type of surgical procedures and findings, the microscopic finding, the immunohistochemical staining and the clinopathological features were analyzed. Results: There were 17 men and 7 women with a mean age of 65 (range: 45∼81). Pathologically, 11 cases were benign, 9 were borderline and 4 were malignant. The tumor was located in the head of the pancreas in 17 patients. 16 cases received Whipple’s procedure and pylorus preserving pancreaticoduodenectomy, and the others were received different kinds of operations. Regarding the subtypes of IPMT, 2 cases were the main duct type, 19 were the branched type and 3 were the combined type. There were no statistically significant differences in the clinical manifestations, radiologic findings and immnohistochemical staining between the patients with benign and malignant IPMT. Except two patients who were not followed up, all the patients had no recurrence and they survived. Conclusion: It is very difficult to exactly differentiate malignant IPMT from benign IPMT with using the current preoperative evaluations and immunohistochemical staining of the resected specimens. The patients who were operated on and followed in our hospital had no recurrence and they all survived. Therefore, if IPMT is suspected, we think the patients should be operated on and we should continue studying other specific antibodies for immunohistochemical staining.

위 말트(Mucosa-Associated Lymphoid Tissue) 림프종의 치료 후 장기성적: 단일기관 연구

Seong Hyun Koh,Seung Hyun Yeo,Moo In Park,Kyoungwon Jung,Sung Eun Kim,Won Moon,Seun Ja Park 대한상부위장관ㆍ헬리코박터학회 2024 Korean Journal of Helicobacter Upper Gastrointesti Vol.24 No.1

Objectives: Few studies have reported long-term follow-up after treatment of gastric mucosaassociated lymphoid tissue (MALT) lymphoma. In this single-center study, we investigated longterm treatment outcomes in patients diagnosed with gastric MALT lymphoma. Methods: The study included 80 patients diagnosed with gastric MALT lymphoma, who were followed up at a single center between January 2005 and December 2019 after Helicobacter pylori eradication therapy, radiotherapy, or chemotherapy. We evaluated complete remission, improvement, or recurrence of the lesion. Follow-up over >60 months was classified as long-term follow-up, and the progression-free survival rate was recorded. Results: Following H. pylori eradication treatment, complete remission occurred in 85.9% (55/64) of H. pylori-positive and 50.0% (3/6) of H. pylorinegative patients. All patients with gastric MALT lymphoma who did not respond to H. pylori eradication therapy (100.0% [6/6]) achieved complete remission following administration of local radiotherapy. We observed no deaths on long-term follow-up (>60 months), and the progression-free survival was 101 months. Conclusions: In this study, patients with gastric MALT lymphoma showed excellent survival rates, progression-free survival, and prognosis on long-term follow-up. Prospective studies are warranted to determine the long-term prognosis of gastric MALT lymphoma after treatment.

Comparison of outcome between liver resection, radiofrequency ablation, and transarterial therapy for multiple small hepatocellular carcinoma within the Milan criteria

Joo Hyun Oh,Dong Hyun Sinn,Gyu-Seong Choi,Jong Man Kim,Jae-Won Joh,Tae Wook Kang,Dongho Hyun,Wonseok Kang,Geum-Youn Gwak,Yong-Han Paik,Joon Hyeok Lee,Kwang Cheol Koh,Seung Woon Paik,Moon Seok Choi 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.4

Purpose: Although surgical resection is usually considered for a single tumor, several reports have suggested that resection can be considered for multiple tumors. The objective of this study was to determine whether resection could provide better long-term outcome for patients with multiple hepatocellular carcinomas (HCCs) within Milan criteria. Methods: A total of 276 patients with multiple HCCs within Milan criteria with liver function preserved who underwent resection, radiofrequency ablation (RFA), or transarterial chemoembolization (TACE) between 2009 and 2013 were analyzed. Propensity-score (PS) matching was conducted. Results: Five-year overall survival (OS) and recurrence-free survival (RFS) were better in the resection group than that in the RFA or TACE group. Patients who underwent resection had more preserved liver function and different tumor characteristics compared to those received RFA or TACE. With similar baseline characteristics generated in the PS model, there was no difference in 5-year OS among 3 groups (79.5% vs. 72.3% or 62.0%, P = 0.232), but the 5-year RFS was better for patients who received resection than those who received RFA or TACE (51.9% vs. 22.0% or 0.0%, P < 0.001). Although the major complication rate was slightly higher than RFA or TACE, there was no significant difference between the 3 groups before and after PS matching. Conclusion: Resection was associated with better RFS than RFA or TACE and showed comparable OS in multiple HCC patients within the Milan criteria, but at a cost of slightly increased risk of complication. Resection can be considered as a first-line option if selected appropriately.