Angiotensin Concerting Enzyme 유전자의 유전적 다형성(Genetic Polymorphism)이 관상동맥 질환 위험인자에 따른 혈중 Plasminogen Activator Inhibitor-1의 농도에 미치는 영향

김덕경,김종원,권현절,류재춘,박승우,김준수,이상훈,홍경표,박정의,이원로 대한고혈압학회 1996 대한고혈압학회지 Vol.2 No.2

항혈청으로 처치된 질트리코모나스(Trichomonas vaginalis) 미세구조의 변화

민득영,류재숙,안명희,박승정,조휘율 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.2

Trichomonas vaginalis is a parasitic flagellate found in the urogenital tract of humans. The present study was performed to evaluate the effect of antiserum from rat and rabbit on fine structure of T. vaginalis. Trophozoites were subcultured in TPS-1 medium(Diamond, 1968) at 37℃, CO₂incubator. T. vaginalis were inoculated in mixed medium containing of antiserum for 10 and 30 minutes, then observed with transmission and scanning electron microscope. The results are as follows: 1. T. vaginalis was ellipsoidal or ovoidal in shape with 4 flagella, an undulating membrane and an axostyle. The plasma membrane of body surface was smooth and furrowed. Pseudopodia(filopods) were often observed by SEM. Ultrastructure of trophozoites consisted of an elliptical shaped nucleus, RERs and Golgi complex around the nucleus, hydrogenosomes, glycogen granules, polysomes and vacuoles in cytoplasm. As the organelles for supporting and movement, an axostyle, kinetosomes, flagella, undulating membrane, costa and parabasal filaments were observed. 2. T. vaginalis treated with antiserum, its surface appeared destruction of plasma membrane, alteration and/or disappearance of axostyle, flagella and undulating membrane under the SEM and showed fine structural changes such as the nucleus surrounded by many vacuoles, decreased ribosomes, which attached to nuclear membrane and RER, vesiculated RER and Golgi complex. The area of filamentous cell coat and a number of coated vesicles were increased on plasma membrane with partial damage. According to going by the incubation time, the progressive destructions of cytoplasmic organelles, nuclear membrane, hydrogenosomal membrane, kinetosome and rootlet fiber were noted. With above results, it is presumed that specific antiserum induced the disturbance of protein systhesis and the damage of cytoplasm resulting lysis of T. vaginalis.

인공방광대치술을 받은 환자에서의 대사 산증 발생

김새인,이동현,김광현,류동열,김승정,강덕희,최규복,이신아 이화여자대학교 의과학연구소 2015 EMJ (Ewha medical journal) Vol.38 No.3

Objectives: Metabolic acidosis frequently develops in patients after neobladder reconstruction. However, the incidence of metabolic acidosis in patients with neobladder and the factors associated with the development of metabolic acidosis have not been well elucidated. We aimed to investigate the incidence and the potential predictors for the development of metabolic acidosis after neobladder reconstruction with intestinal segment. Methods: We included patients who underwent neobladder reconstruction using intestinal segment at Ewha Womans University Mokdong Hospital between January 1, 2005 and December 31, 2014. A subgroup of patients according to the time of metabolic acidosis occurrence was further analyzed in order to characterize predictors for metabolic acidosis. Results: Metabolic acidosis was encountered in 79.4% of patients with neobladder during follow up period. When patients were divided into 2 groups according to anion gap (AG), total CO2 (18.9±2.1 mEq/L vs. 20.0±1.3 mEq/L, P=0.001) and chloride (106.6±4.9 mE/L vs. 109.4±3.6 mEq/L, P<0.001) were significant different between groups with AG>12 and AG≤12. Furthermore, when patients were divided into 3 groups; patients with metabolic acidosis at postoperative day (POD) 1; from POD 2 to 14 days; after 14 days, there was significant difference among those subgroups. Conclusion: Our study showed the rate of metabolic acidosis in patients underwent neobladder reconstruction and the difference between patients with metabolic acidosis and those without metabolic acidosis for the first time in Korea. In the future, well designed prospective study will be needed to prevent metabolic acidosis after neobladder reconstruction.

Association between vascular access failure and microparticles in hemodialysis patients

( Duk Hee Kang ),( Seung Jung Kim ),( Kyu Bok Choi ),( Dong Ryeol Ryu ),( Jung Hwa Ryu ),( Su Young Lim ) 대한신장학회 2012 Kidney Research and Clinical Practice Vol.31 No.1

Background: Vascular access failure, a major cause of morbidity in hemodialysis (HD) patients, occurs mainly at stenotic endothelium following an acute thrombotic event. Microparticles (MPs) are fragments derived from injured cell membrane and are closely associated with coagulation and vascular inflammatory responses. Methods: We investigated the relationship between levels of circulating MPs and vascular access patency in HD patients. A total of 82 HD patients and 28 healthy patients were enrolled. We used flow cytometry to measure endothelial MPs (EMPs) identified by CD31þCD42 or CD51þ and platelet-derived MPs (PMPs) identified by CD31þCD42þ in plasma samples of participants. Vascular access patency was defined as an interval from the time of access formation to the time of first access stenosis in each patient. MP counts were compared according to access patent duration. Results: The levels of EMP (both CD31þCD42 and CD51þ) and CD31þCD42þPMP were significantly higher in patients than in healthy participants. Levels of CD31þCD42 EMP and CD31þCD42þPMP showed a positive correlation. In nondiabetic HD patients, CD31þCD42 EMPs and CD31þCD42þPMPs were more elevated in the shorter access survival group (access survival o1 year) than in the longer survival group (access survival Z 4 years). Conclusion: Elevated circulating EMP or PMP counts are influenced by end-stage renal disease and increased levels of EMP and PMP may be associated with vascular access failure in HD patients.

Two Cases of Emphysematous Cystitis in Maintenance Hemodialysis Patients (초)

( Seung Jung Jun ),( Mi Na Yu ),( Hei Rim Ahn ),( Dong Ryeol Ryu ),( Seung Jung Kim ),( Kyu Bok Choi ),( Duk Hee Kang ) 대한내과학회 2010 대한내과학회 추계학술발표논문집 Vol.2010 No.-

Expression of peroxisome proliferator-activated receptor (PPAR)-${\alpha}$ and PPAR-${\gamma}$ in the lung tissue of obese mice and the effect of rosiglitazone on proinflammatory cytokine expressions in the lung tissue

Ryu, Seung Lok,Shim, Jae Won,Kim, Duk Soo,Jung, Hye Lim,Park, Moon Soo,Park, Soo-Hee,Lee, Jinmi,Lee, Won-Young,Shim, Jung Yeon The Korean Pediatric Society 2013 Clinical and Experimental Pediatrics (CEP) Vol.56 No.4

Purpose: We investigated the mRNA levels of peroxisome proliferator-activated receptor (PPAR)-${\alpha}$, PPAR-${\gamma}$, adipokines, and cytokines in the lung tissue of lean and obese mice with and without ovalbumin (OVA) challenge, and the effect of rosiglitazone, a PPAR-${\gamma}$ agonist. Methods: We developed 6 mice models: OVA-challenged lean mice with and without rosiglitazone; obese mice with and without rosiglitazone; and OVA-challenged obese mice with and without rosiglitazone. We performed real-time polymerase chain reaction for leptin, leptin receptor, adiponectin, vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-${\alpha}$, transforming growth factor (TGF)-${\beta}$, PPAR-${\alpha}$ and PPAR-${\gamma}$ from the lung tissue and determined the cell counts and cytokine levels in the bronchoalveolar lavage fluid. Results: Mice with OVA challenge showed airway hyperresponsiveness. The lung mRNA levels of PPAR${\alpha}$ and PPAR-${\gamma}$ increased significantly in obese mice with OVA challenge compared to that in other types of mice and decreased after rosiglitazone administeration. Leptin and leptin receptor expression increased in obese mice with and without OVA challenge and decreased following rosiglitazone treatment. Adiponectin mRNA level increased in lean mice with OVA challenge. Lung VEGF, TNF-${\alpha}$, and TGF-${\beta}$ mRNA levels increased in obese mice with and without OVA challenge compared to that in the control mice. However, rosiglitazone reduced only TGF-${\beta}$ expression in obese mice, and even augmented VEGF expression in all types of mice. Rosiglitazone treatment did not reduce airway responsiveness, but increased neutrophils and macrophages in the bronchoalveolar lavage fluid. Conclusion: PPAR-${\alpha}$ and PPAR-${\gamma}$ expressions were upregulated in the lung tissue of OVA-challenged obese mice however, rosiglitazone treatment did not downregulate airway inflammation in these mice.

파벨라 증후군: 총비골신경 마비의 흔치 않은 원인

신덕섭(Duk-Seop Shin),조창우(Chang-Woo Cho),류승민(Seung Min Ryu) 대한정형외과학회 2017 대한정형외과학회지 Vol.52 No.5

파벨라(비복근두종자골, fabella)는 의학적으로 많이 중요하지 않게 여겨진다. 파벨라가 총비골신경 마비의 원인 중에서 한 가지가 될 수 있다는 보고는 거의 없었다. 본 증례에서는 파벨라가 있는 66세의 농부가 갑자기 신경학적 증상을 호소하였다. 수술적 절제술을 시행하였고 신경학적 증상이 극적으로 회복되었다. 최근에 총비골신경 마비를 동반한 파벨라 증후군에 관한 보고는 거의 없었고, 국내에는 아예 없었다. 그래서 저자들은 파벨라가 총비골신경 마비를 일으킬 수 있다는 점에 대해 보고하고자 한다. The fabella has been considered to have a minor clinical significance. However, there have been a few reports describing the fabella as one of the causes of peroneal nerve palsy (PNP). This report describes a 66-year-old farmer who complained of acute neurologic symptoms involving the fabella. Surgical extirpation was performed and neurologic function was recovered dramatically. Recently, there have been no published reports on fabella syndrome with common PNP. Here, we report an unusual case of common PNP with the fabella as a cause.

LDL-Cholesterol 직접측정법에 의한 한국인 LDL-C 참조치 설정에 관한 연구

이승관,서장훈,정운원,이창규,김상섭,류정록,김덕수,김석수 高麗大學校 倂設 保健大學 保健科學硏究所 1996 保健科學論集 Vol.22 No.1

The concentration of low-density lipoproteins cholesterol(LDL-C) in serum is the basis for the classfication and treatment of hypercholesterolemia. Due to the inherent complexity of measuring LDL by the reference method, ultracentrifugation, it usually is estimated by Friedewald calculation that uses measured values for total cholesterol, high-density lipoprotein(HDL) cholesterol, and triglycerides. Calculated LDL(CLDL) results may be in accurate, especially if a patient's triglyceride level is elevated, and an important limitation of Friedewald equation is the need for a fasting sample to estimate LDL-C in serum. Recently a new "direct" immunological procedure for determining LDL-C has been developed and the estimation of LDL-C using this method is now increasing in our country. In this study we have analyzed reference values of direct LDL-C obtained from some healthy Korean by means of AIC method Following results were obtained 1. In total group, it had shown that the optimal model for normal distribution was the power function(f(x)=X^(0.56) ) with reference range of 56∼179㎎/dl. 2. In total men group, the power function(f(x)=X^(0.81)) with reference range of 55∼176㎎/dl was found as the optimal model showing normal distribution 3. In total women group, the power function(f(x)=X^(0.06)) with reference range of 60∼187㎎/dl was found as the optimal model for normal distribution 4. Power function were the predominant one in all age groups, of both sexes, with reference ranges of 57∼162, 54∼180, 55∼181 and 45∼178㎎/dl in 30s, 40s, 50s and 60s of male and those of female were 55∼177, 60∼192, 69∼175 and 58∼176㎎/dl respectively.

풍력발전이 SMP에 미치는 영향

류승현(Ryu, Seung-Hyun),엄신영(Um, Shin-Young),김수덕(Kim, Su-Duk) 한국신재생에너지학회 2009 한국신재생에너지학회 학술대회논문집 Vol.2009 No.06

제4차 전력수급기본계획(2008-2022)과 제3차 신 재생에너지 기술개발 및 이용.보급계획에 의거하여 전력산업기반기금을 통해 우선 구매되는 풍력발전이 계통한계가격(SMP, System Marginal Price)에 미치는 영향을 점검하였다. 제4차 전력수급기본계획(2008-2022)에서 향후의 전원설비건설계획과 전력수요를 참고하고, 제3차 신 재생에너지 기술개발 및 이용 보급계획에 의거, 풍력발전보급계획을 반영하였다. 발전변동비의 변화는 미래의 화석연료변동에 따를 것으로 보아 명목상승률 3%, 6%, 그리고 8%의 경우를 검토하였다. 현재는 풍력발전용량이 전체발전용량에 비해 미미해서 SMP에 큰 변화를 가져다주지 못하고 있지만 2014년에는 SMP가 결정되는 구간이 LNG발전기에서 유연탄발전기로 옮겨감에 따라 SMP차이가 최대 20.02[/KWh](발전 비용 명목 상승률8%)에 달하다가 2020년에는 다시 0[/KWh](발전비용 명목상승률8%)으로 줄어드는 것으로 분석되었다.

악성 골종양의 진단적 접근

신덕섭(Duk-Seop Shin),류승민(Seung-Min Ryu),박철현(Chul-Hyun Park) 대한정형외과학회 2015 대한정형외과학회지 Vol.50 No.6

악성 골종양은 원발성 악성 골종양, 속발성 악성 골종양 및 전이성 골종양으로 나눌 수 있다. 골종양은 매우 드문 질환으로 성인의 원발성 악성 골종양은 전체 암의 1% 정도이고, 소아에서는 약 6% 정도를 차지한다. 통증이 없는 종괴를 주소로 하는 악성 연부조직 종양과는 달리 대부분의 악성 골종양 환자는 통증을 주소로 내원하게 된다. 과거 병력, 가족력 등에 대한 문진과 촉진 및 신체 검사를 한 뒤 증상과 관련된 방사선 사진을 먼저 촬영하게 되고, 경험이 풍부한 근골격계 종양의사는 진단에 대한 단서를 상당 부분 이 단순 방사선 사진에서 얻게 된다. 정확한 진단과 병기 결정을 위해 병리학적 진단, 종양의 국소 범위(local extent of tumor)와 원격전이 여부를 알아야 하며, 이를 위해 핵의학 검사, 전산화 단층촬영(computed tomography, CT), 핵자기공명영상(magnetic resonance imaging) 및 양전자방출 단층촬영(positron emission tomography-CT) 등의 영상검사와 생검이 필요하다. 만일 환자의 임상소견과 단순 방사선 사진이 공격적인 진행을 하는 양성 골종양이나 악성 골종양이 의심되면, 더 이상의 진단적 영상검사나 조직검사를 하지 말고 경험이 많은 정형외과 종양전문의에게 전원하여야 한다. Malignant bone tumors would be classified as primary malignant bone tumors, secondary malignant bone tumors, and metastatic bone tumors. Primary malignant bone tumors are rare diseases occupying 1% of adult cancers, and 6% of pediatric cancers. The chief complaint of malignant bone tumor patients is pain different from that of malignant soft tissue tumor patients. Diagnostic procedures start with clinical evaluation including current illness, past medical history, family history, and physical examination. Then we take a radiograph first and obtain important and diagnostic clues from it. However pathological diagnosis and information about the extent of tumor are required to obtain a more definite diagnosis and staging. Examinations for detection of local and systemic tumor extent are scintigraphy, computed tomography (CT), magnetic resonance imaging, and positron emission tomography-CT. If the clinical and radiographic information suggests aggressive or malignant bone tumor, the patient should be referred to a bone tumor specialist without further evaluations.