유방선암의 유형에 따른 c-erbB-2, p53, bcl-2, 그리고 c-myc의 발현

강영근,조현득,오미혜,김의한,양승하 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

Breast carcinoma is a common malignant tumor in female of the korea. Various oncogenes have been found in malignant tumor cells. There were many reports or correlation between the prognosis of patients and c-erbB-2, p53, bcl-2, and c-myc, but is controversial. We performed immunohistochemical stain for above antigenic protein for 80 cases of breast carcinoma and evaluated expression rate of each antigens, and we compared their correlation with prognosis of patients. The results were as follow; 1) The expression rates in breast carcinoma was c-erbB-2 80%, p53 56.3%, bcl-2 51.3% and c-myc 53.8%. 2) c-erbB-2 showed no significant difference according to histologic types, grade, and lymph node metastatic status of breast carcinoma. 3) p53 showed higher expression rate in metastatic cases compared to non-metastatic cases, and it showed higher expression rate according to increasing of grade without significant difference. There is no significant difference according to types. 4) The expression rate of bcl-2 in breast carcinoma be lowed according to increasing of grade. There was no significant difference according to types, side, and status of lymph node metastasis. 5) c-myc showed higher expression rate in non-metastatic cases compared to and metastatic cases, and it showed decreasing expression rate according to increasing of grade without significant difference. There is no significant difference according to types. 6) p53 and bcl-2 in breast carxinoma showed reverse corre1ation(P < 0.05), and bc1-2 and c-myc showed close correlation without statiscally significance. Above results showed that p53 may be related to poor prognosis of the patients and c-myc may be related good prognosis in breast carcinoma. There was reverse correlation between p53 and bcl-2 and bcl-2 and c-myc may be correlated closely. c-erbB-2 and bcl-2 showed no interrelation to prognosis.

일부 흡연 대학생의 지각된 스트레스와 흡연실태에 대한 상관관계 연구

강초록,권시내,권혜림,박수경,송인혜,오해인,이미지,한승아,황선아,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

In spite of the seriousness of smoking harm has been developed these days. the increase of national smoking rate and that the people in the twenties are especially leading the rise of national smoking rate is an important issue of national health. Smoking of the university students can occur health problem and cause loss of manpower and expenses in their social activity period. So then the actual state for prevention of smoking and development of a program to prohibit smoking has to be seized urgently and starting with the study of the realities of university students, the effort to find a related fluent of smoking has to be studied. Related to this, the purpose of this study is to seize the general characteristics of smoking university students and to find out the relationship between perceived stress and smoking status among smoking university students. In addition, by collating the relationship between perceived stress and smoking status among smoking university students we have the purpose to furnish the basal data of stress care, the best way to cope with stress through group training and the plan for nursing implementation to decrease the smoking rate. For this study, a survey was conducted for 204 smoking university students of 13 universities in Seoul from 9th of May to 16th of May, 2003. The collected data was analyzed by t-test, Pearson Correlation and ANOVA in the statistical packages SPSS/PC. Major findings of this study are as follows; 1. Of the 204 respondents 85.4% of them were male and 14.6% of them were female. The average age of the sample was 23.19 years old and 41.8% of them were in senior, 21.4% in junior, 16.9% in sophomore, 19.9% in freshman grade. The major motivation to start smoking was 'because of curiosity'(33.8%) and the first smoking experience was at 'senior high school' (41.9%) and at 'college' (41.9%). 2 The highest scored stress factor of smoking university students were stresses about school work. 3. 72.7% had experience to quit smoking at least one or more times. Most of the reason to keep on smoking was 'short will to quit'(51.0%) and the smoking place was usually at 'school' (41.4%). Most respondents for the number ofsmoking friends were '5 people and above' (73.2%). 4. There was a negative relationship between the general characteristics and the entire perceived stress, however, there was a positive relationship between the general characteristics and the perceived stress in 4 categories which is 'sex distinction', 'major of study', 'residence form' and' inmate'. 5. There was a negative relationship between the general characteristics and the entire smoking status, however, there was a positive relationship between the general characteristics and the smoking status in 2 categories which is 'presence of hobby' and 'number of smoking friends'. 6. There was a negative relationship between the perceived stress and the smoking status. Through this study we have seized the relationship between the general characteristics, perceived stress and the smoking status of smoking university students. There was a positive relationship between some of the general characteristics with the perceived stress and the smoking status of smoking university students. Also there was a negative relationship between the perceived stress and the smoking status.

전혈에서의 트리플루살에 의한 혈소판 응집억제 효과

이혜승,강경수,이병철,유경호 대한신경과학회 1997 대한신경과학회지 Vol.15 No.1

임신 중 파열된 담관낭종 1예

강병철,김영우,정혜경,이선영,이한주,손혜영,성순희,김승철 대한소화기학회 2000 대한소화기학회지 Vol.35 No.2

Choledochal cysts are congenital dilatation of the biliary tree occurring predominantly in female. Most of the cases are diagnosed during childhood, and its presentation during pregnancy or postpartum period is rare. Choledochal cysts during pregnancy are frequently associated with complication due to the concealment of the mass by enlarged uterus. Rupture of cyst is a rare complication that occurs spontaneously in most cases. We experienced a case of ruptured choledochal cyst during pregnancy in a 28-year-old primigravida. It occurred at 32 weeks of pregnancy. A premature newborn was delivered by normal vaginal delivery. The choledochal cyst was excised, and reconstruction was carried out by Roux-en-Y choledochojejunostomy. Although choledochal cysts occurring in pregnancy are rare, it is important to be aware of the condition because a missed or delayed diagnosis may be catastrophic for both mother and child.

감마선 조사 육류, 가금류에서 저장전과 후의 조사선량에 따른 DNA fragmentation의 변화

이혜진,김상미,박유경,양재승,강명희 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.2

The changes in DNA damage were investigated during storage after irradiation. Beef, pork and chicken were irradiated at 1.0, 3.0 and 5.0 kGy and stored for 6 months at 20℃. The comet assay was applied to the sample muscles at the beginning of irradiation and at the end of storage. Muscles were isolated, sliced, and the suspended cells were embedded in an agarose layer. After lysis of the cells, they were electrophoresed for 2 min. and then stained. DNA fragmentation in tissues caused by irradiition was quantified as tail length and taii moment (tail length x % DNA in tail) by comet image analyzing system. Right after irradiation, the differences in tail length between unirradiated and irradiated muscles were significant(p<0.05) in beef, pork and chicken. With increasing the irradiation doses, statistically significant longer extension of the DNA from the nucleus toward anode was observed. Similarly even 6 months after irradiation, all the irradiated muscles significantly showed longer taii length than the unirradiated controls. The results represented as tail moment showed similar tendency to those of tail length, but the latter parameter was more sensitive than the former. These results indicate that the comet assay could be one of the simple methods of detecting irradiated muscles. Moreover, this method suggest that using comet assay, we were able to detect DNA damage differences even after 6 months after irradiation.

거대 자궁 근종의 1예

전혜숙,강경화,이보연,이선경,허주엽,김승보 경희대학교 2000 慶熙醫學 Vol.16 No.3-4

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

Whole-genome resequencing analysis of 20 Micro-pigs

Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.

A rare case of extensive papillary muscle calcification in senile cardiac calcification syndrome

김은진;송봉근;강민호;손형래;홍수민;박동원;허승혜;김계연;최석구 인제대학교 2011 仁濟醫學 Vol.32 No.-

광범위 유두근 석회화는 흔하지 앉으며, 원인과 임상적 중요성에 관한 문헌만 드물게 찾아볼 수 있다. 반면에 작은 석회 침착은 노인에서 흔하며 심장 첨부에 주로 나타난다. 유두근 석회화는 관상동맥질환, 확장성 심근병증, 승모관 질환, 고칼슘혈증, 그리고 말기신부전에서 인산칼슘의 중가와 연관성이 있다. 저자는 심장초옴파와 MDCT를 통해 진단된 전외측 유두근의 광범위 석회화의 드문 사례가 있어 보고한다.

교정용 브라켓과 호선 간의 마찰 저항력의 비교

서충환,정혜승,조진형,강경화 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.2

본 연구는 고정성 장치를 이용한 교정 치료에서 브라켓과 교정용 호선 사이의 활주 이동 동안 발생하는 마찰력에 디자인과 재질이 다른 각각의 브라켓과 여러 종류의 호선, 다양한 브라켓-호선 각도 등이 어떤 영향을 미치는가를 알아보기 위하여 4종의 브라켓(stainless steel twin 브라켓인 Gemini^(?), 좁은 근원심 폭경과 single bracket의 디자인이 포함된 Mini Uni-Twin^(?), metal-reinforced 세라믹 브라켓인 Clarity^(?), 세라믹 브라켓인 Transcend^(?)을 사용하고, 3종의 교정용 호선(0.016˝. 0.016×0.022˝ stainless steel호선, 0.016˝Nitinol)을 이용하여 브라켓-호선 각도를 0˚, 3˚, 9˚로 조절하면서 실험한 결과, Gemini^(?)는 유의하게 가장 낮은 정지, 운동 마찰력을 보였으며(P<0.001), Clarty^(?)는 0˚의 브라켓-호선 각도에, Transcend^(?)는 6˚와 9˚의 각도에서 유의하게 가장높은 정지, 운동 마찰력을 보였고(P<0.001), 0.016×0.022˝ stainless steel 각형 호선은 유의하게 가장 높은 정지, 운동 마찰력을 보였으며(P<0.01),0.016˝ stainless steel 원형호선은 0˚와3˚의 브라켓-호선 각도에서(P<0.01), 0.016˝ Nitinol은 6˚와9˚의 각도에서 (P<0.001)유의하게 가장 낮은 정지, 운동마찰력을 보였고 브라켓-호선 각도가 증가함에 따라 유의하게 정지, 운동마찰력도 증가 하였다(P<0.001) The object of this study was to evaluate how friction that occurs during the sliding movement of an orthodontic archwire through orthodontic brackets is differently affected by variant designs and ingredients of brackets and archwires and bracket-archwire angles. In order to simulate the situations which could occur during orthodontic treatment with fixed appliances, 4 types of brackets(Gemini^(?), a stainless steel twin bracket:Mini Uni-Twin^(?), a stainless steel bracket with a single bracket design and narrow mesio-distal width: Clarity^(?), a metal-reinforced cermic braket: T ranscend^(?), a ceramic bracket)and 3 types of orthodontic archwires (0.016˝, 0.016×0.022 stainless steel, 0.016˝ Nitinol)were used and the bracket-archwire angles were controlled as 0˚,3˚,6˚, and 9˚, Gemini^(?) significantly showed the lowest static and kinetic frictions (P<0.001). Clarity showed the highest static and kinetic frictions with a bracket-archwire angle of 0˚, and Transcend at 6˚ and 9˚(P<0.001). An 0.016×0.022˝ stainless steel rectangylar archwire significantly showed the highest static and kinetic frictions(P<0.01). The lowest static and kinetic frictions were observed when the bracket-archwire angles were 0˚ and 3˚ with 0.016˝ stainless steel round archwires(P<0.01), and 6˚ and 9˚ with 0.016 Nitinol(P<0.001). The static and kinetic frictions were increased as the bracket-archwire angles were increased(P<0.001).