서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

시설재배 작물의 적정 시비량 결정을 위한 간이 검정법

홍순달,임태준,강성수,양원석 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

토양의 질산태 질소 함량이 67㎎ ㎏-1에서 343 ㎎ ㎏-1의 분포를 갖는 8개 시설재배 토양에서 시비수준을 무비구와 표준시비구로 하여 오이의 생산력을 검정하고 토양 화학성과의 상호관계 및 회귀관계의 평가로부터 질소 적정 시비량 추천모델을 추정하였다. 토양의 질산태 질소 함량과 무비구 오이의 생체중, 비료효과 및 시비효율의 상호관계를 Cate & Nelson 분할법에 의하야 추정한 질소 시비의 상한기준은 280㎎ ㎏-1으로 추정되었다. 토양의 질산태 질소 수준에 따른 표준시비구의 생체중 변화의 관계로부터 토마토 질소 표준시비를 위한 질산태 질소 하한기준은 100㎎ ㎏-1으로 추정되었다. 따라서 상한기준과 하한기준 사이의 질산태 질소 검정에 의한 토마토, 오이, 배추의 질소 시비량 추천모델은 다음과 같이 개발하였다. 질소 표준시비량에 대한 비율 Y=-0.5556X +155.568. 재배기간이 장기간인 오이의 질소 덧거름 시비량 결정방법을 확립하기 위하여 ChlorophyⅡ meter (SPAD502, Minolta)를 활용한 엽록소 검정법을 검토하였다. 평가방법은 NO3-N 함량이 67∼343㎎ ㎏-1인 8개 토양에서 무비구 오이의 생체중과 경시적 엽위별 엽록소 측정치와의 회귀관계로부터 오이의 생산능력과 질소공급능력에 대한 엽록소 측정치의 기준을 추정하였다. 오이 재배기간 동안 엽위별 엽록소 측정치는 정식 후 39일째 13매 잎에서 최대를 보인 후 감소되었으며, 그 이후 오이의 엽록소 측정치는 토양의 질소 공급능력에 따라 현저한 차이를 나타냈다. 정식 후 39일째 13매엽의 엽록소 측정치는 토양의 질산태 질소, 무비구 오이의 생체중 및 질소 흡수량, 시비효율과 유의성 있는 상관관계를 보였다. 그리고 오이 최대 생체중에 대한 부족비율은 13매엽 엽록소 측정치와 유의성 있는 부의 상관을 보였으며 엽록소 최대치에 대한 부족비율과는 곡선적인 정의 상관을 보였다. 따라서 오이 최대 생체중에 대한 부족비율을 덧거름 요구량이라고 가정하면 13매엽의 엽록소 측정치는 덧거름의 사용시기와 기준을 결정할 수 있는 가능성을 보였다. 따라서 시설재배 오이의 밑거름은 질산태 질소의 토양검정에 의하여 결정하고 덧거름은 오이 정식 후 39일째 13매엽의 엽록소 측정으로 결정할 수 있는 종합적인 질소 시비추천 방안을 확립하였다.

공침법에 의한 BiDy₂Fe_(5)O_(12) 결정형 나노입자 분말의 제조 및 응용 연구

홍영대,조정환,김태엽,전성욱,홍영명 고신대학교 자연과학연구소 2007 고신대학교 자연과학연구소 논문집 Vol.14 No.-

금속산화물들은 각종 산업재료로서 유용하며 더욱이 그것들의 결정형 나노입자를 경제적으로 제조하는 기술은 21세기 첨단산업에 매우 중요하다. 저자들은 前報에서 화학합성법에 의한 각종 금속산화물의 나노입자를 제조하는 표준공정법을 연구하여 보고하였다. 본 연구에서는 그 표준공정법을 이용하여, 가넷의 일종인 BiDy_(2)Fe_(5)O_(12) 결정 나노입자 분말을 제조하고 그것의 자성체로의 응용을 위해 제품특성을 조사하었다. 가네트 제조과정에 필요한 침전물은 제품설계에 의거 몰비로 계산된 Bi, Dy, Fe의 질산염들의 혼합수용액에 침전제로서 pH NH_(3)-NH_(4)CI 완충용액 일정량을 가하여 얻어졌다. 이렇게 제조된 침전은 열처리과정을 거처 BiDy_(2)Fe_(5)O_(12) 결정형 나노입자 분말로 만들어졌다. 얻어진 제품의 입자크기, 조성, 결정구조 및 광자기성 등 특성시험이 SEM, ICP, XRD, 그리고 VSM 등을 사용하여 실시되었다. 각종 분석법을 사용하여 제품의 특성을 조사한 결과, 최초에 설계한 대로의 자성체가 나노 크기의 분말로 얻어졌으며 이것을 통하여 전보에서 확립한 표준공정법의 유효성도 입증되었다. Metallic oxides are useful as various industrial materials. Futhermore, the cost-effective techniques of fabricating the crystalline nanopartides of metallic oxides are very important for the high-tech industries of the 21 century, In the previous study, the authors reported the standard process for the preparation of crystalline nanoparticles of metallic oxides by chemical synthesis, In this study, BiDyzFesOiz nanoparticles were prepared by using the reported standard process and the characteristics of the product were investigated for applicability as magnetic substance. The precipitation needed for manufacturing process of garnet was made by adding the NH_(3)-NH_(4)CI buffer solution of pH 10 to the mixed solution of bismuth (III) nitrate, dysprosium(III) nitrate and iron(III) nitrate. The amount of each nitrate was calculated by the designed mole ratios. The nano-size crystalline powders of BiDy_(2)Fe_(5)O_(12) were earned by heating the precipitation at 800℃ for 4 hours in air. The characterizations, such as particle size, composition, crystal structure and magneto-optical property of garnet were tested by SEM, ICP, XRD, and VSM analyses. The results of the analyses showed the following two facts. First, a good magnetic substance of BiDy_(2)Fe_(5)O_(12) was earned as crystalline nano-size powders according to the original design. Next, the established standard process for the fabrication of METALLIC oxides nanonartieles was approved as a very effective method.

중소기업 마케팅 생산성 향상을 위한 인터넷 활용 의도 형성과정 : 기술수용모델의 적용 Applying the Technology Acceptance Model

홍성태 한국생산성학회 2004 生産性論集 Vol.18 No.3

This study investigates the process of the Internet usage of the small and medium sized enterprises with the technology acceptance model. Results show that the perceived usefulness and the perceived ease of use mediate the intention of the Internet utilization of the SMEs. Also, it is found that the product appropriateness to the Internet, the resource barrier, the intensity of the industry competition make a significant effect on the perceived usefulness of the Internet and organizational Internet knowledge significantly influences the perceived ease of the Internet use. We also found that the perceived ease of use affects indirectly the intention through the perceived usefulness. Some theoretical and practical implications are suggested.

情報的 네트워크로서의 國際마케팅 組織의 進化 : 本社-子會社間의 情報技術의 活用을 中心으로

홍성태 釜山外國語大學校國際通商硏究所 1998 國際經營論集 Vol.13 No.-

The economic condition of a country influences all over the world on a real time basis. Multinational enterprises which operate under this circumstance should design and develop the fittest organization from to catch worldwide irregular symptoms and trends. Especially the development of information and transportation technology enables them to accomplish this task. The realization of the importance of information makes organizations structured in the way to get the information flow easier. The purpose of this paper is to review the development process of international marketing organizations to face turbulent environment and to find the strategic intent of network organization supported by information technology. Conclusions drawn from this study are as follows. First, the relationship between a headquarters and subsidiaries of companies of companies which seek to globalization shifted from hierarchical relation to network relation. Second, through utilizing information technology in the headquarters and subsidiaries will be enhanced and the more local market-oriented activities will be executed. Third, information technology can be used as a means to promote interorganizational learning and gain competitive advantage. Fourth, resent environmental changes are to require a headquarters to control subsidiaries through information. Lastly, sharing common values between a headquarters and subsidiaries is highly needed to gain competitive advantage.

일 농촌지역에서 발기부전의 유병률 및 상관 요인

홍진표,송해철,이무송,이창화,안준호,한오수,안태영,김성윤 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The purpose of this study was to investigate the prevalence of erectile dysfunction (ED) in a rural area of Korea and to examine the relationship between the prevalence and correlates of subjects. Method : This study was done as a part of the chronic disease survey of rural area by the Asan Foundation. Among 30 years or older population from Jung Eup, a rural area of Korea, 711 male subjects were sampled using a cluster probability sampling method. Trained interviewers visited and interviewed subjects aged 30 years or older with a structured interview. ED was categorized as 'none', 'mild', 'moderate', or 'severe' according to the ability to 'attain and/or maintain an erection satisfactory for sexual intercourse'. Response rate was 54.3% (N=386). Results : The age weighted prevalence of ED was 27.2% (minimal 14.1%, moderate 7.6%, severe 5.5%). Prevalence and severity increased with age. Liver disease and underweight were significantly (p<0.05) associated with ED. Smoking seemed to be correlated with ED, but did not reach statistically significant level. Conclusion : ED was highly prevalent in the rural community of Korea. Medical, sociodemographic, and lifestyle variables associated with ED may alert physicians to patients at risk for ED and offer insight to its etiology.

骨格筋 Protein Methylase Ⅱ에 關한 硏究

洪承元,郭相太,林圭,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

Protein methylase Ⅱ (S-adenosy-L-methionine : protein-carboxyl 0-methyltransferase) has been, purifed 6,600-fold from bovine skeletal muscle and characterized. The purification procedures involved ammonium sulfate fractionation, Sephadex G-75 chromatography, S-adenosyl-L-homocysteine-Sepharose 4B chromatography, and hydroxyapatite chromatography. The enzyme showed an optimal pH around 6.0. It was heat-labile, being inactivated by heattreatment at 60℃ for 6 minutes. However, storage of the enzyme at -20℃ in the presence of 10% glycerol prevented the activity loss for 6 months. The apparent Km for S-adenosyl-L-methionine was 2.86 × 10 exp (6) M. Copper(Cu^2+) ion was potent noncompetitive inhibitor, the activity being completely inhibited b) 0.25 mM Cu^2+ and 1 mM Zn^2+. Most of the activity loss by copper and zinc ions were recovered by the addition of ethylenediamine tetracetate. The enzyme was also completely inhibited by 0.5 mM phydroxymercuribenzoate, which could be reversed by the addition of 10 mM 2-mercaptoethanol. The molecular weight of the enzyme was 24,000, as determined by Sephadex G-75 chromatography. Histone IIA, immunoglobulin A and soybean trypsin inhibitor were efficiently carboxylmethylated by the enzyme. Sarcoplasmic reticulum(SR) and sarcolemma(SL) of the skeletal muscle were also efficiently carboxylmethylated by the enzyme at a rate of 0.96 and 0.63 pmolesl mg protein/min of 3^H-CH_3, incorporation, respectively. Polyacrylamide gel electrophoresis of the carboxylmethylated SR and SL revealed multiple carboxylmethylated subfactions : SR resolved into the subfractions with molecular weight 100,000, 55,000, 22,000 and 14, 400, and SL into the subfractions with molecular weight 48,000, 22,000 and- 14, 400. Of these subfractions those with Mr=22, 000 were most strongly carboxylmethylated. Endogenous cAMP-dependent phoshorylation of SR occurred in the subfractions with Mr=55, 000, Mr=27, 000 and Mr=~14, 400, of which the fraction with Mr=27, 000 was most strongly phosphorylated. When SR was carboxylmethylated, the endogenous cAMP-dependent phosphorylation was decreased. SR exhibited relatively high Ca^2+-ATPase activity and oxalate-facilitated ATP-dependent 45^Ca^z+uptake. The carboxylmethylation and cAMP-dependent phosphorylation of SR increased the Ca^2+TATPase activity by 15% and by 29%, respectively. However, cAMP-dependent phosphorylation of SR which had been previously carboxylmethylated led to only 23% increase in the Ca^2+-ATPase activity. Both the carboxylmethylation and cAMP-dependent phosphorylation enhanced the steady-state rate as well as the initial rate of ^45Ca^2+-uptake. Kinetic analysis showed that the apparent Km and Vmax of Ca^2+ for ATP-dependent Ca^2+-uptake were 45μM and 35 nmoles/mg protein, respectively. Both the carboxylmethylation and cAMP-dependent phosphorylation of SR decreased the Km and increased the Vmax for the Ca^2+-uptake. These results suggest that protein methylase II might have some regulatory role in the calcium transport of sarcoplasmic reticulum.

생물변환 천연식용색소 생산공정기술 개발 : Ⅰ. 홍화 황색소의 안정성에 미치는 pH 및 온도의 영향 Ⅰ. Effect of pH and Temperature on the Stability of the Yellow Pigment from Carthamus tinctorious L.

홍성현,박성길,이종민,한태룡,백영숙,정인식 경희대학교 생명자원과학연구원 1999 硏究論文集 Vol.20 No.-

홍화로부터 열에 의한 안정성을 검토하였다. 황색소의 분해반응은 산성, 중성, 염기성 조건에서 UV/Vis spectral measurement에 의해 조사되었다. 시간변화에 다른 흡광도를 측정해 본 결과 온도가 상승함에 따라 황색소의 분해되는 정도가 점차적으로 증가하는 것을 확인 할 수 있었다. 40℃에서 황색소의 분해 반감기는 pH3.0, pH7.0, 그리고 pH12.0에서 각각 112.1, 39.3 과 25.3 시간이었다. 이것은 황색소가 pH3.0에서 비교적 더 안정함을 말한다. pH3.0, 7.0 그리고 12.0에서 황색소의 activation energy는 각각 519, 15.0 그리고 12.3 kJ/mol이었다 We investigated thermal stability of yellow pigment from Carthamus tinctorious L. Decomposition reactions of yellow pigment were monitored at acidic, neutral and alkaline conditions by UV/Vis spectral measurement. Decomposition of yellow pigment increased as temperature increased Decomposition half lives of yellow pigment at 40℃ were 112.1 39.3, and 25.3 hrs at pH 3.0 , pH 7.0, and pH 12.0, respectively, indicating that yellow pigment is more stable at pH 3.0. The activation energies of yellow pigment at pH 3.0, pH 7.0, and pH 12.0 were 51.9, 15.0, and 12. 3 kJ/mol, respectively.

A Change of Cyberspace and Social Movement-Focusing on the Disciplining

Hong,Seong-Tae 비판사회학회 2003 경제와 사회 Vol.- No.58

표본화 및 유지회로 성능향상에 관한 연구

홍국태,이성대,정강민 成均館大學校 科學技術硏究所 1995 論文集 Vol.46 No.1

In this paper, a high speed, high accuracy sample and hold circuit that minimizes the hold step error by using a new circuit architecture is proposed. This new sample-and-hold scheme takes advantages of the speed of the open loop sample-and-hold circuit in the SAMPLE time and the cancellation of charge injection in the HOLD time. It operates above 50MHz with 0.3mV hold step error.