들깨 유전자원의 단백질함량과 지방 및 지방산 특성

홍성택,손석용,노창우,이경희,정재현,박재성 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

공시된 들깨 지방재래종 90개 품종의 단백질함량, 지방 및 지방산을 분석한 결과 다음과 같다. 1. 숙기별로는 중ㆍ만생종의 평균단백질 함량은 22.4%로 비교적 낮았고, 품종분포가 대부분 평균치에 가까웠으며, 조생종은 평균단백질 함량이 24.1%로 비교적 높았다. 2. 평균단백질함량은 소립종(천립중3.0~4.0g이하)이 23.0%로, 중립종(천립중 4.0~5.0g)이나 대립종(천립중 5.0~6.0g)의 22.1%보다 높은 경향이었다. 3. 기름함량은 조생종이 42.6%로 가장 적었고, 중생종이 44.5%, 만생종이 45.5%로 기름함량이 많았으며, 립중별로는 소립종이 44.2%, 중립종이 43.1%, 대립종이 41.5% 대립종일수록 낮은 경향이었다. 4. 지방산조성은 숙기가 늦어짐에 따라 Oleic acid와 Linolenic acid는 다소 많았고, Linoleic acid는 적어지는 경향이었다. Linoleic acid는 극대립종에서 15.5%로 가장 높았고 립중이 가벼울수록 높아지는 경향이었다. 5. 기름함량과 지방산 비율간의 상관 정도를 보면 기름함량과 Palmitic acid, Linoleic acid는 負의 상관이나, Stearic acid, Linolenic acid는 正의 상관을 보였다. Stearic acid는 Oleic acid와 유의적인 정의 상관을 , Linoleic acid와는 부의 상관을 보였다. Oleic acid는 Linoleic acid 및 Linolenic acid와 고도로 유의한 부의 상관을, Linoleic acid는 Linolenic acid와, 포화지방산은 불포화지방산과 유의한 부의 상관을 보였다. Korean local collected strains or varieties of perilla analyzed on their protein and oil content and fatty acids composition in the seed. The strains analyzed were 90 of which grown in the experimental field of Chungbuk Agricultural Research and Extension Services at Cheongwon and Umseong from 2001 to 2002. The seed protein contents of 90 perilla varieties ranged from 19.1% to 29.0% and the average content was 22.8%. There differed by the seed coat color showing 1.9% higher content in light gray than that of dark brown, which may suggest that the seed coat color could be used as a marker gene for breeding high protein varieties. Oil content of medium and late varieties in maturity showed higher as compared to early varieties, and appeared differences by seed coat color, and small seed varieties showed higher seed oil content than in large seed varieties by seed size. Linoleic and linolenic acid content of which major fatty acids in perilla seed were 74.4% in average of tested varieties. Fatty acid composition of perilla varieties were not significantly different by maturity, seed coat color and 1,000 seed weight. Oil content showed high positive correlation, with stearic and linolenic acids and negative correlation with linoleic acid.

[논문] trn L-trn F에 의한 한국 족도리풀속 식물 종 분류

성혜경,허만규,허홍욱 釜山大學校 師範大學 科學敎育硏究所 2011 科學敎育硏究報 Vol.38 No.-

Asarum consists low-growing herbs and is a genus in the Aristo-lochiaceae family with species found in the north temperate zones with most species in Asia. Any species with a highly structured variation pattern must show different distributions of phenotypic characteristics at different locations. I analysed intra- and interspecific phylogenic relationships within genus Asarum in Korea. I evaluated a representative sample of the fourteen taxa (eight Korean taxa and six obtained taxa from GenBank) with the trn L intron and the trn L - trn F spacer region of the chloroplast genome to estimate genetic relationships within genus Asarum. No variable positions were found within the trn L 5` exon and trn L 3` exon. Variable positions within the intron region were mostly distributed outside the structural elements required for intron processing, i.e., not within the first 140 bp at the 5` end and not within the 90 bp at the 3` end of the intron region. Within the spacer region, variable positions were evenly distributed. Alignment of the DNA sequences required adding numerous gaps. Sequence variation within the Asarum is mostly due to nucleotide substitutions, although several small indels and 20 inserts can be found. Another source of sequence divergence is length variation due to stretches of short repeats that occur at the intergenic spacer region ITS2 in all the Asarum. A + T content for eight Korean species of genus Asarum ranged between 64.3% and 64.6%. The base furtherance did not showed the significant difference to the by a total taxon. These values are similar to those (64.5%~67.1%) for the angiosperm alignments of the total trn L and trn F region. The main phylogenetic analysis revealed two distinct clades. The first clade includes ten species (eight Korean species, A. nipponicum, and A. tamaensis). The second clade includes four species (A. canadense , A. cautatum, A. chingchengense , and A. yakusimense). However, some internal nodes are poorly supported. Within Korean Asarum, four species were unsolved portions.

상어 간유 흡인에 의한 급성 지방성 폐렴 1예 : 증례보고

임성경,옥철호,김희규,장태원,정만홍 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

저자들은 상어 간유를 과량 복용하고 기침, 호흡곤란을 주소로 내원한 48세 여자 환자에서 방사선 소견과 기관지 폐포 세척액 검사를 통해 급성 지방성 폐렴으로 진단하고 스테로이드에 불응하는 경과를 취하자 우측 전폐 기관지 폐포 세척술을 시행하여 폐 병변과 증상의 호전을 보인 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Acute lipoid pneumonia is uncommon condition of the lungs that results from the aspiration of vegetable, animal, or mineral oils. Shark liver oil (so called Squalene) is taken as a traditional remedy in some Asian countries, and is used widely also in cosmetics. It results in foreign body type inflammatory reaction of the lung and can show various clinical presentations from asymptomatic incidental finding to severe pneumonia leading to acute respiratory failure. Although many cases have been reported, severe lipoid pneumonia requiring steroid or whole lung lavage for treatment is rare. We experienced a case of acute lipoid pneumonia of 48 year old female with dyspnea and cough caused by regular ingestion of large amount Shark liver oil, treated by whole lung lavage. We reported this case with a brief review of related literatures.

Metallo-β-lactamase 생성 Pseudomonas aeruginosa 및 포도당 비발효그람음성간균의 검출방법에 관한 연구

신경섭,김원식,손재철,홍승복,최재운,형성민 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: Metallo-β-lactamse (MBL)는 carbepenem를 포함하는 대부분의 β-lactam 항균제를 가수분해할 수 있으므로 MBL 생성균의 확산은 이들 균에 의한 감염의 치료에 큰 문제를 야기할 수 있다. 따라서 MBL 생성균에 의한 감염의 확산을 막기 위해 이들 균의 조기 검출이 필요하다. 저자들은 MBL 생성균을 검출할 수 있는 세 가지 방법을 PCR 방법과 비교하여 보았다. 재료 및 방법: Imipenem의 MIC가 8 μg/mL 이상인 50개의 포도당 비발효 그람음성 간균을 대상으로 EDTA-double disk synergy test (EDTA-DDS), Etest MBL, Hodge 변법을 시행하였으며 대조 검사로 IMP-1, VIM-1, VIM-2에 대한 PCR을 시행하였다. 결과: 총 50 균주 중 MBL 생성균은 Pseudomonas aeruginosa 1 균주, Alcaligenens xylosxidans 7 균주 등 8 균주(16%)가 검출되었다. 이들 균주는 모두 VIM-2 형이었으며, EDTA-DDS, Etest MBL은 PCR 방법과 100% 일치하였다. Hodge 변법은 8 MBL 생성균주 중 2 균주를 검출하지 못했다. 결론: 50 균주 중 8 균주가 MBL 생성균이었고 모두 VIM-2 형이었다. Etest MBL은 가격이 비싸 검사실에서는 EDTA-DDS가 적당할 것으로 사료된다. Purpose: Because metallo-β-lactamase (MBL) has activity to hydrolysls against most β-lactam drugs, dissemination of MBL producing bacteria may cause problems of treatment of infection by their microorganisms. Early detection or screening for MBLs will contribute to prevent further spread of resistance. Authors compared three MBL detection methods with PCR for MBLs. Materials and Methods: For 50 isolates of g1ucose nonfermentative gram negative bacilli with reduced susceptability against imipenem (MIC≥8 μg/mL), EDTA-double disk synergy (EDTA-DDS), Etest MBL and modified Hodge test were compared with PCR detection method for MBLs. Results: Among 50 isolates of glucose nonfermentative microorganisms, eight MBL producers including a Pseudomonas aeruginosa and 7 Alcaligenes xylosoxidans were detected. Those isolates were VIM-2 producer. The results of EDTA-DDS and Etest MBL completely agreed with PCR for MBL detection. Two isolates among eight MBL Producers were not detected in modified Hodge test. Conclusion: All of MBL producing bacteria were VIM-2 genotype. In conclusion, EDTA-DDS may be useful method for MBL detection in clinical laboratory due to high cost of Etest MBL strip.

비정질 인듐갈룸ᅳ아연-산화물 박막트랜지스터 기반의 AMOLED 화소 회로

이재표(Jae-Pyo Lee),유경민(Kyeong-Min Yu),장진녕(JinNyoung Jang),홍문표(MunPyo Hong),배병성(Byung Seong Bae) 호서대학교 공업기술연구소 2013 공업기술연구 논문집 Vol.32 No.2

본 논문은 비정질 인둠ᅳ갈f ᅳ아연-산화물 박막트랜지스터 (a-IGZO TFT)를 이용하여 능동형 유기발광다이오드 (AMOLED)용 문턱전압(Vth )을 보상하는 화소 회로를 제안하였다. 산화물 TFT는 n-채널 TFT로써, 우리는 n-채널 TFT 특성으로 회로를 최적화하였다. 제안된 화소 회로는 회로 시뮬레이션 뿐만 아니라 회로 분석을 이용하여 확인되었다. 제안된 화소 회로는 AMOLED에서 구동 TFT의 문턱전압 변화를 보상할 수 있다. 제안된 화소 회로를 이용함으로써, 문턱전압 보상은 달성되었다. rhis paper proposes a tnresnold voltage compensation pixel circuit for active-matrix organic light-emitting diode (AM OLED) using amorphous indium-gaUiimi-zinc-oxide thin-film transistors (a-IGZO TFTs), Oxide TFT is an n-channel TFT; therefore, we optimized the circuit for the n-channel TFT characteristics. The proposed pixel circuit was verified using circuit analysis as well as circuit simulations. The proposed circuit could compensate for the threshold voltage variations o f drive TFT in AM OLED. Using the proposed pixel circuit, threshold voltage compensation was achieved.

A pH-sensitive excited state intramolecular proton transfer fluorescent probe for imaging mitochondria and <i>Helicobacter pylori</i>

Hong, Kyeong-Im,Park, Seong-Hyun,Lee, Seong Min,Shin, Injae,Jang, Woo-Dong Elsevier 2019 Sensors and actuators. B Chemical Vol.286 No.-

<P><B>Abstract</B></P> <P>An hydroxythiophene-conjugated benzothiazole (<B>T<SUB>2</SUB>(OH)B</B>) was synthesized as a pH sensitive fluorescent probe. <B>T<SUB>2</SUB>(OH)B</B> displayed dual emission behavior due to the excited state intramolecular proton transfer (ESIPT) process, while the methoxy analogs, <B>T<SUB>2</SUB>(OMe)B</B>, did not. <B>T<SUB>2</SUB>(OH)B</B> showed strong fluorescence enhancement above physiological pHs. The applicability of <B>T<SUB>2</SUB>(OH)B</B> as a biological pH indicator was demonstrated by its use for staining mitochondrial compartment and <I>H. pylori</I>. Owing to its strong fluorescence emission with a large stokes shift and its effective response to pH, <B>T<SUB>2</SUB>(OH)B</B> serves as a platform for the design of new types of fluorescent probes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Because <B>T<SUB>2</SUB>(OH)B</B> showed large stokes shift through ESIPT process, it can be useful for fluorescent probe. </LI> <LI> Owing to turn-on fluorescence emission behavior of <B>T<SUB>2</SUB>(OH)B</B> above physiological pHs, it was successfully applied for mitochondria of mammalian cells, as well as detected <I>H. pylori</I> adhered to mammalian cells. </LI> <LI> Also, <B>T<SUB>2</SUB>(OH)B</B> displayed faster staining rates than commercially available MitoTracker red. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

연약지반 매설 가스배관의 응력 모니터링 시스템

홍성경(Seong Kyeong Hong),김준호(Joon Ho Kim),정석영(Sek Young Jeong) 한국가스학회 2005 한국가스학회 학술대회논문집 Vol.2005 No.10

천연가스 기화설비의 내진성능평가

홍성경(Seong Kyeong Hong),김준호(Joon Ho Kim),최원목(Won Mog Choi),박영호(Young Ho Park),김민수(Min Soo Kim) 한국강구조학회 2015 韓國鋼構造學會誌 Vol.27 No.6

천연가스 생산기지 Pipe Rack의 내진성능평가

홍성경(Seong Kyeong Hong),김준호(Joon Ho Kim),최원목(Won Mog Choi),박영호(Young Ho Park),김민수(Min Soo Kim) 한국강구조학회 2015 韓國鋼構造學會誌 Vol.27 No.6

A New Wheat Variety, “Jojoong” with Pre-harvest Sprouting Resistance, Early Maturity, High Yield and Good Noodle Quality

Chon-Sik Kang,Kyeong-Hoon Kim,Young-Keun Cheong,Jae-Han Son,Jong-Chul Park,Kyong-Ho Kim,Kwang-Geun Park,Ouk-Kyu Han,Gi-Heung Hong,Jin-Kyeong Choi,Seong-Tae Lee,Jeong-Suk Bae,Bo-Kyeong Kim,Chulsoo Park 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

“Jojoong”, a winter wheat(Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Suwon272/Olgeuru//Keumkang/Suwon252” during 2002. “Jojoong” was evaluated as “Iksan360” in advance yield trial test in 2011. It was tested in the regional yield trial test between 2012 and 2014. “Jojoong” is an awned, semi-dwarf and hard winter wheat, similar to “Keumkang” (check cultivar). The heading and maturing date of “Jojoong” were earlier to “Keumkang”. “Jojoong” had lower test weight (799 g/L) and 1,000-grain weigh (35.6g) than “Keumkang” (816 g/L and 45.5g, respectively). “Jojoong” showed resistance to winter hardiness and pre-harvest sprouting, which lower withering rate on the high ridge (10.5%) and rate of pre-harvest sprouting (10.5%) than “Keumkang” (31.7 and 21.4%, respectively). “Jojoong” showed similar protein content (12.5%), SDS-sedimentation volume (43.5ml) and gluten content (8.6%) to “Keumkang” (12.9%, 58.5ml and 8.5%, respectively). It showed higher lightness (93.17) in flour color than “Keumkang” (91.95, respectively). “Jojoong” showed higher lightness (81.50) of noodle dough sheet than “Keumkang” (80.95). “Jojoong” exhibited similar hardness (3.84N) and higher springiness and cohesiveness of cooked noodles (0.94 and 0.66) compared to “Keumkang” (3.88N, 0.90, and 0.62, respectively). Average yield of “Jojoong” in the regional adaptation yield trial test was 5.09 MT/ha in upland and 5.35 MT/ha in paddy field, which was 9% and 8% higher than those of “Keumkang” (4.67 MT/ha and 4.92 MT/ha, respectively).