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분사처리 후 산부식 표면처리된 교정용 미니 임플랜트의 골유착능에 관한 연구
전미선,강윤구,모성서,이근혜,국윤아,김성훈 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.5
본 연구에서는 교정 치료 시 골내 고정원으로 사용되는 교정용 임플랜트의 표면처리 여부가 골유착능에 있어서 어떠한 효과를 보이는지 제거회전력의 측정을 통해 알아보고자 하였으며, 그에 따른 교정력 적용의 확장과 임상적 의의를 알아보고자 하였다. 실험군은 분사처리 후 산부식(Sand-blasted Large grit, and Acid etched, SLA) 표면 처리된 교정용 미니 임플랜트인 C-implant (Cimplant, Seoul, Korea)를 사용하였으며 대조군은 같은 디자인이지만 표면 처리를 하지 않은 평활면 C-implant를 사용하였다. 실험군과 대조군을 각각 2개씩 11마리의 가토 경골에 식립하였고 식립 후 6주에 가토를 희생시켜 제거회전력을 측정하여 t-test를 통하여 두 군의 제거회전력 차이의 통계적 유의성을 알아보았으며 조직표본을 만들어 조직소견을 관찰하였다. 실험결과 제거회전력은 SLA 처리한 C-implant 군이 평활면 C-implant 군보다 통계적으로 유의성 있게 높은 결과를 보였다 (p < 0.05). 평활면 C-implant 군의 평균 제거회전력 값은 4.614 Ncm이고, SLA C-implant 군의 평균 제거회전력 값은 6.286 Ncm로, SLA 군이 평활면 군보다 73% 더 높은 제거회전력에 대한 저항성을 나타내었다. 이상의 연구 결과에서 SLA 표면처리가 C-implant의 골유착능을 증가 시켰음을 알 수 있었다. 따라서 표면 처리된 교정용 미니 임플랜트는 기존의 임플랜트에 비해 좀 더 강한 힘에 저항할 수 있으며 탈락률을 낮출 수 있을 것으로 생각된다. Objective: The purpose of this study was to compare the torque resistance to removal of sandblasted large grit and acid etched (SLA) surface treated orthodontic mini-implants and smooth surface orthodontic mini-implants as well as performing histologic observations. Methods: Two groups of custom screw shaped orthodontic mini-implants (C-implant, 1.8 mm outer diameter × 9.5 mm length, Cimplant, Seoul, Korea) were designated. 22 SLA treated C-implants (SLA group) and 22 machined surface C-implants (machined group) were placed in the tibia metaphysis of 11 adult New Zealand white rabbits. Following a 6-week healing period, the rabbits were sacrificed. Subsequently, the C-implants were removed under reverse torque rotation with a digital torque measuring device and independent t-test was performed. Selected tissues were prepared for histologic observation. Results: The SLA group presented a higher mean removal torque value (6.286 Ncm) than the machined group (4.491 Ncm) which was statistically significant (p < 0.005). Histologic observation revealed a trend of more new bone formation in contact with the screw surface in the SLA group than the smooth group. Conclusions: The results of this study suggested that SLA surface treatment can enhance the osseintegration potential for C-orthodontic mini-implants.
Seong Kook Jeon,Jin Suk Park,Jeong Gu Kang,Sun Hee Kim,Nan Ee Lee,Jung Mi Lee,Subin Moon,Dae In Ha,Do Yon Kim,Jeong-Heon Ko,Yong-Sam Kim 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01
Genome editing technology promises to provide versatile tools for the generation of various model cell lines, plants and animals as well as for gene therapy by gene-editing in a target-specific manner. To date, three distinct modes of genome editing technologies have been introduced and extensively investigated in experimental settings, and attempted for use in clinical settings. Despite the revolutionized efficiency and sophistication in gene editing owing to development of CRISPR/Cas9, there remains technical limitations for currently available programmable nucleases in the routine use in experimental and clinical settings. Here, we introduce a universal genome editing technology (UGET) that relies on gene targeting through simple base pairings between a 20-28 nt nucleotide probe and a stretch of single-strand target DNA at the replication fork. UGET has no target limitations, shows lower-than-expected off-target incidence, is straightforward to use, and is compatible to gene correction via HDR. In particular, an overall construct size is less than 2.3kb, which means a high flexibility for use in gene therapy using viral vectors including adenovirus-associated viruses as well as for agrobacterium-based transformation in plants.
Cleavage of double-stranded DNA by engineered FokⅠendonuclease
Seong Kook Jeon,Yong-Sam Kim,Sun Hee Kim,Jeong-Heon Ko 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
Genome editing is one of the most demanding tools in mammalian cells to unravel the role of genes and to implement gene therapy of genetic diseases. Zinc finger nuclease is an engineered restriction enzyme which is constructed by combining an array of zinc finger DNA-binding motifs in the N- terminus and the FokⅠ endonuclease in C-terminus. Almost every ZFN recognizes three consecutive nucleotides starting with guanine in a context-dependent manner, thereby limiting the use of ZFN in genome editing. To address this challenge, we replaced ZFNs with a gene-specific nucleotides, which were connected to FokⅠ nuclease by using (his)6-tag and GS linkers. We tested in vitro whether this engineered FokⅠ shows a site-specific nuclease activity. For this, the engineered FokⅠ and nucleotide complex was incubated with template DNA which was produced from 70-mer and 100-mer nucleotides base-paired each other. The results indicate that the complex showed a site-specific nuclease activity, which suggests that the nucleotide-based FokⅠ can be used for the universal genome editing.
Cleavage of double-stranded DNA by engineered FokI endonuclease
Seong Kook Jeon,Yong-Sam Kim,Sun Hee Kim,Hyang-Sook Yoo,Jeong-Heon Ko 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Genome editing is one of the most demanding tools in mammalian cells to unravel the role of genes and to implement gene therapy of genetic diseases. Zinc finger nuclease is an engineered restriction enzyme which is constructed by combining an array of zinc finger DNA-binding motifs in the N-terminus and the FokΙ endonuclease in C-terminus. Almost every ZFN recognizes three consecutive nucleotides starting with guanine in a context-dependent manner, thereby limiting the use of ZFN in genome editing. To address this challenge, we replaced ZFNs with a gene-specific nucleotides, which were connected to FokΙ nuclease by using (his)6-tag and GS linkers. We tested in vitro whether this engineered FokΙ shows a site-specific nuclease activity. For this, the engineered FokI and nucleotide complex was incubated with template DNA which was produced from 70-mer and 100-mer nucleotides base-paired each other. The results indicate that the complex showed a site-specific nuclease activity, which suggests that the nucleotide-based FokΙ can be used for the universal genome editing.
Jeon, Yoon-Sun,Cha, Jae-Kook,Choi, Seong-Ho,Lee, Ji-Hyun,Lee, Jung-Seok Korean Academy of Periodontology 2020 Journal of Periodontal & Implant Science Vol.50 No.5
Purpose: This study was conducted to analyze specific RNA expression profiles in gingival tissue and saliva samples in periodontitis patients and healthy individuals, and to determine their correlations in light of the potential use of microarray-based analyses of saliva samples as a periodontal monitoring tool. Methods: Gingival tissue biopsies and saliva samples from 22 patients (12 with severe periodontitis and 10 with a healthy periodontium) were analyzed using transcriptomic microarray analysis. Differential gene expression was assessed, and pathway and clustering analyses were conducted for the samples. The correlations between the results for the gingival tissue and saliva samples were analyzed at both the gene and pathway levels. Results: There were 621 differentially expressed genes (DEGs; 320 upregulated and 301 downregulated) in the gingival tissue samples of the periodontitis group, and 154 DEGs (44 upregulated and 110 downregulated) in the saliva samples. Nine of these genes overlapped between the sample types. The periodontitis patients formed a distinct cluster group based on gene expression profiles for both the tissue and saliva samples. Database for Annotation, Visualization and Integrated Discovery analysis revealed 159 enriched pathways from the tissue samples of the periodontitis patients, as well as 110 enriched pathways In the saliva samples. Thirty-four pathways overlapped between the sample types. Conclusions: The present results indicate the possibility of using the salivary transcriptome to distinguish periodontitis patients from healthy individuals. Further work is required to enhance the extraction of available RNA from saliva samples.