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Yun, Seok Joong,Jeong, Pildu,Kang, Ho Won,Kim, Ye-Hwan,Kim, Eun-Ah,Yan, Chunri,Choi, Young-Ki,Kim, Dongho,Kim, Jung Min,Kim, Seon-Kyu,Kim, Seon-Young,Kim, Sang Tae,Kim, Won Tae,Lee, Ok-Jun,Koh, Gou-Yo Korean Continence Society 2015 International Neurourology Journal Vol.19 No.2
<P><B>Purpose:</B></P><P>MicroRNAs (miRNAs) in biological fluids are potential biomarkers for the diagnosis and assessment of urological diseases such as benign prostatic hyperplasia (BPH) and prostate cancer (PCa). The aim of the study was to identify and validate urinary cell-free miRNAs that can segregate patients with PCa from those with BPH. </P><P><B>Methods:</B></P><P>In total, 1,052 urine, 150 serum, and 150 prostate tissue samples from patients with PCa or BPH were used in the study. A urine-based miRNA microarray analysis suggested the presence of differentially expressed urinary miRNAs in patients with PCa, and these were further validated in three independent PCa cohorts, using a quantitative reverse transcriptionpolymerase chain reaction analysis. </P><P><B>Results:</B></P><P>The expression levels of hsa-miR-615-3p, hsv1-miR-H18, hsv2-miR-H9-5p, and hsa-miR-4316 were significantly higher in urine samples of patients with PCa than in those of BPH controls. In particular, herpes simplex virus (hsv)-derived hsv1-miR-H18 and hsv2-miR-H9-5p showed better diagnostic performance than did the serum prostate-specific antigen (PSA) test for patients in the PSA gray zone. Furthermore, a combination of urinary hsv2-miR-H9-5p with serum PSA showed high sensitivity and specificity, providing a potential clinical benefit by reducing unnecessary biopsies. </P><P><B>Conclusions:</B></P><P>Our findings showed that hsv-encoded hsv1-miR-H18 and hsv2-miR-H9-5p are significantly associated with PCa and can facilitate early diagnosis of PCa for patients within the serum PSA gray zone.</P>
Wavelet Based Multi-Bit Fingerprinting Against Geometric Distortions
Kim, Won Gyum,Seo, Yong Seok,Jung, Hye Won,Lee, Seon Hwa,Oh, Won Geun Trans Tech Publications, Ltd. 2006 Key Engineering Materials Vol.321 No.-
<P>This paper presents a new image fingerprinting scheme which embeds a multi-bits fingerprinting code and is robust against the geometric attack such as rotation, scaling and translation. We construct a 64 bits fingerprinting code and embed into wavelet subband of 512x512 images repeatedly. In order to restore an image from geometric distortion a noise reduction filter is performed and a rectilinear tiling pattern is used as a template. Results of experimental studies show that our method is robust against geometric distortions and JPEG compression.</P>
Expression of the Blue Fluorescent Protein (AmCyan) in the Fibroin of Transgenic Silkworms
Seon Young Kim,Seong Wan Kim,Eun Young Yun,Kwang-Ho Choi,Seong Ryul Kim,Seok Woo Kang,Seung Won Park,Tae Won Goo 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04
To product the blue fluorescent protein (AmCyan) expressed cocoon, we were fused AmCyan cDNA to the heavy chain gene and injected the gene into a silkworm. AmCyan was one of the existing violet fluorochromes and originally derived from the fluorescent protein amFP486. AmFP486 was cloned from the sea anemone Anemonia majano (GenBank accession number AF168421), and belongs to the family of fluorescent proteins (FPs) isolated from coral reef organisms. The AmCyan fusion protein, each with N- and C- terminal sequences or the fibroin H-chain, were designed to be secreted into the lumen of the posterior silk glands. The expression of the AmCyan/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3 EGFP as a marker allowed us to rapidly distinguish transgenic silkworm. Mixtures of the donor and helper vectors were micro-injected into 300 eggs of bivoltine silkworms (Baegokjam). EGFP fluorescence was observed in 3 broods of transgenic silkworms under a florescence stereomicroscope. The cocoon was displayed strong blue fluorescence, proving that the fusion protein was present in the cocoon. Accordingly, we suggest that the AmCyan gene expressed cocoon will be enable the production of the novel biomaterials based on the transgenic silk.
Endoscopic Cryotherapy of Lung and Bronchial Tumors: A Systematic Review
( Seon Heui Lee ),( Won Jung Choi ),( Sook Whan Sung ),( Young Kyoon Kim ),( Chi Hong Kim ),( Jae Il Zo ),( Kwang Joo Park ) 대한내과학회 2011 The Korean Journal of Internal Medicine Vol.26 No.2
Background/Aims: We made a systematic review and evaluation of endoscopic cryotherapy of endobronchial tumors, investigating safety and efficacy. Methods: Qualified studies regarding endoscopic cryotherapy of lung tumors were systemically evaluated using available databases according to predefined criteria. Results: In total, 16 publications were included in the final assessment. A narrative synthesis was performed because a formal meta-analysis was not viable due to the lack of controlled studies and study heterogeneity. Overall success rates for significant recanalization of the obstruction were approximately 80%, although they varied, depending on disease status in the patient population. Complications from the procedure developed in 0-11.1% of cases, most of which were minor and controlled by conservative management. Although limited data were available on comprehensive functional assessment, some studies showed that respiratory symptoms, pulmonary function tests, and performance status were significantly improved. Conclusions: Endoscopic cryotherapy was found to be a safe and useful procedure in the management of endobronchial tumors although its efficacy and appropriate indications have yet to be determined in well-designed controlled studies. (Korean J Intern Med 2011;26:137-144 )
Kim, Jin-Hyang,Won, Dong-Seon,Lee, Ju-Yeon Korean Chemical Society 2008 Bulletin of the Korean Chemical Society Vol.29 No.1
2,5-Di-(2'-hydroxyethoxy)-4'-nitrostilbene (3) was prepared and polycondensed with terephthaloyl chloride, adipoyl chloride, and sebacoyl chloride to yield novel T-type polyesters (4-6) containing the NLO-chromophores dioxynitrostilbenyl groups, which constituted parts of the polymer backbones. Polymers 4-6 are soluble in common organic solvents such as acetone and N,N-dimethylformamide. They showed thermal stability up to 260 oC in thermogravimetric analysis with glass-transition temperatures obtained from differential scanning calorimetry in the range 90-95 oC. The second harmonic generation (SHG) coefficients (d33) of poled polymer films at the 1064 nm fundamental wavelength were around 1.42 ´ 10-9 esu. The dipole alignment exhibited high thermal stability up to 5 oC higher than glass-transition temperature (Tg), and there was no SHG decay below 100 oC due to the partial main-chain character of polymer structure, which is acceptable for NLO device applications.
Vaccine-type mutations identified in Varicella zoster virus passaged in cell culture
Kim, Seok Cheon,Won, Youn Hee,Park, Ji Seon,Jeon, Jeong Seon,Ahn, Jin Hyun,Song, Moon Jung,Shin, Ok Sarah,Lee, Chan Hee Elsevier 2018 Virus research Vol.245 No.-
<P><B>Abstract</B></P> <P>Varicella-zoster virus (VZV) is a causative agent for chickenpox and shingles. Comparative genomic sequence analysis of clinical and vaccine strains suggested potential sites responsible for attenuation. In this study, low and high passages of two VZV clinical strains cultured in human fibroblast cells were compared for genomic DNA sequences and growth characteristics. Mutations were detected at 187 and 162 sites in the strain YC01 and YC02, respectively. More than 86% of mutations were found in open reading frames, and ORF62 exhibited highest frequency of mutations. T to C and A to G transitions accounted for more 90% of all possible substitutions. Forty mutations were common to two strains, including 27 in ORF62. Mutations found in attenuated vaccine strains were also detected at 7 positions. Both high and low passage strains were infectious and grew similarly in human fibroblast cells. In guinea pig cells, however, high passage strain remained infectious while low passage strain lost infectivity. This study may provide new insight into the attenuating mutations associated with <I>in vitro</I> passaging of VZV.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mutations accompanied with <I>in vitro</I> passaging in cell culture of VZV were analyzed. </LI> <LI> Most of the mutations were T to C or A to G substitution. </LI> <LI> ORF62 and NCR62/63 were the main target of cell culture-associated mutations. </LI> <LI> Seven vaccine-type mutations were detected in high-passage strain of VZV. </LI> <LI> Adaptation of high-passage strain of VZV to unnatural host cell was observed. </LI> </UL> </P>