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The effect of cycle tourism on the quality of life
Won-Jae Seo,Joo-Young Jang,Yong-Eun Kim,Seung-JIn Han 한국스포츠과학회 2018 스포츠과학연구(JSAS) Vol.2 No.1
Today, the population of cycle participants is consistently increasing. Cycling is becoming not only a form of physical exercise but also a form of tourism in which cycle activities are related to visiting a particular destination. With the perspective of participation sport tourism, the purpose of this study is to investigate how the level of cycle tourists’ participation affects their quality of life. An online survey link was sent to cycle participants to collect data. Out of the total 337 respondents, a total of 226 samples were used for the final analysis, disregarding those that were found to be inadequate or unreliable. For data analysis, frequency analysis, reliability test, validity, correlations, and regressions were employed with SPSS 22.0. For our first result, we found that the level of cycle participation did not significantly affect the quality of physical life. Second, the level of cycle participation did not have significant effect on the quality of mental life. Third, the level of cycle participation behavior did not have a significant effect on the quality of social life. Fourth, the level of cycle participation behavior did not have a significant effect on the quality of environmental life. Conclusions and political implications are discussed.
Management of Focal Chondral Lesion in the Knee Joint
Seo, Seung-Suk,Kim, Chang-Wan,Jung, Dae-Won The Korean Knee Society 2011 대한슬관절학회지 Vol.23 No.4
<P>Articular cartilage does not contain vascular, nervous and lymphatic tissue and chondrocytes hardly participate in the healing or repair process of chondral tissue because of being surrounded by plenty of extracellular matrix. Therefore, the injury to articular cartilage frequently requires an operative treatment. The goal of surgical repair of articular cartilage is to regenerate nearly normal chondral tissue and prevent degenerative arthritis caused by the articular cartilage defect. Microfracture is a kind of cartilage repair procedure that makes a fibrin clot containing mesenchymal stem cells in the chondral lesion. Microfracture is a simple procedure but it has a disadvantage that the repaired tissue is fibrocartilage. Autologous chondrocyte implantation has an advantage that it implants fully differentiated chondrocytes to the lesion, which theoretically produces hyaline cartilage. Its disadvantages are that it is a two stage and a costly procedure. Osteochondral autograft transplantation is a one stage procedure and repairs the lesion with hyaline cartilage. But its limitation is the lack of donor site availability. Surgeons who understand the theoretical background, indications, surgical methods, rehabilitation, complications, and clinical course of cartilage repair procedures can achieve the goal of preventing degenerative arthritis.</P>
Seung Won Kang,Chul-Hee Lee,Sang-Gyu Seo,Bal-Kum Han,Hyung-Seok Choi,Sun Hyung Kim,Chee Hark Harn,Gung Pyo Lee 한국원예학회 2011 원예과학기술지 Vol.29 No.2
To identify unintended vertical gene-transfer rates from the developed transgenic plants, rapid and unequivocal techniques are needed to identify event-specific markers based on flanking sequences around the transgene and to distinguish zygosity such as homo- and hetero-zygosity. To facilitate evaluation of zygosity, a polymerase chain reaction technique was used to analyze a transgenic pepper line B20 (homozygote), P915 wild type (null zygote), and their F1 hybrids, which were used as transgene contaminated plants. First, we sequenced the 3’-flanking region of the T-DNA (1,277 bp) in the transgenic pepper event B20. Based on sequence information for the 3’- and 5’-flanking region of T-DNA provided in a previous study, a primer pair was designed to amplify full length T-DNA in B20. We successfully amplified the full length T-DNA containing 986 bp from the flanking regions of B20. In addition, a 1,040 bp PCR product, which was where the T-DNA was inserted, was amplified from P915. Finally, both full length T-DNA and the 1,040 bp fragment were simultaneously amplified in the F1 hybrids; P915 × B20, Pungchon × B20, Gumtap × B20. In the present study, we were able to identify zygosity among homozygous transgenic event B20, its wild type P915, and hemizygous F1 hybrids. Therefore, this novel zygosity identification technique, which is based on PCR, can be effectively used to examine gene flow for transgenic pepper event B20.
Manassantin A and B from Saururus chinensis inhibiting cellular melanin production
Seo, Chang-Seob,Lee, Won-Hee,Chung, Hee-Wook,Chang, Eun Ju,Lee, Seung Ho,Jahng, Yurngdong,Hwang, Bang Yeon,Son, Jong-Keun,Han, Sang-Bae,Kim, Youngsoo John Wiley Sons, Ltd. 2009 Phytotherapy research Vol.23 No.11
<P>Hyperpigmentation disorders such as freckles and senile lentigines in the skin are associated with abnormal accumulation of melanin pigments. In this study, two lignan constituents were isolated from Saururus chinensis Baill (Saururaceae) as inhibitors of cellular melanin production by bioassay-guided fractionations. The active constituents were manassantin A and B that dose-dependently inhibited melanin production in α-melanocyte stimulating hormone (α-MSH)-activated melanoma B16 cells with IC<SUB>50</SUB> values of 13 nm and 8 nm, respectively. Arbutin as a positive control exhibited an IC<SUB>50</SUB> value of 96 µm on α-MSH-induced melanin production. Further, manassantin A inhibited forskolin- or 3-isobutyl-1-methylxanthine (IBMX)-induced melanin production with IC<SUB>50</SUB> values of 14 nm or 12 nm, respectively. Manassantin A decreased cellular amounts of IBMX-inducible tyrosinase protein but could not affect the catalytic activity of cell-free tyrosinase, a key enzyme in the biosynthetic pathway of melanin pigments. Finally, this study could provide a pharmacological potential of S. chinensis in hyperpigmentation disorders. Copyright © 2009 John Wiley & Sons, Ltd.</P>
( Seung-on Lee ),( Ah-won Kwak ),( Mee-hyun Lee ),( Ji-hye Seo ),( Seung-sik Cho ),( Goo Yoon ),( Jung-il Chae ),( Sang Hoon Joo ),( Jung-hyun Shim ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.12
Picropodophyllotoxin (PPT), an epimer of podophyllotoxin, is derived from the roots of Podophyllum hexandrum and exerts various biological effects, including anti-proliferation activity. However, the effect of PPT on colorectal cancer cells and the associated cellular mechanisms have not been studied. In the present study, we explored the anticancer activity of PPT and its underlying mechanisms in HCT116 cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to monitor cell viability. Flow cytometry was used to evaluate cell cycle distribution, the induction of apoptosis, the level of reactive oxygen species (ROS), assess the mitochondrial membrane potential (Δψm), and multi-caspase activity. Western blot assays were performed to detect the expression of cell cycle regulatory proteins, apoptosis-related proteins, and p38 MAPK (mitogen-activated protein kinase). We found that PPT induced apoptosis, cell cycle arrest at the G1 phase, and ROS in the HCT116 cell line. In addition, PPT enhanced the phosphorylation of p38 MAPK, which regulates apoptosis and PPT-induced apoptosis. The phosphorylation of p38 MAPK was inhibited by an antioxidant agent (N-acetyl-L-cysteine, NAC) and a p38 inhibitor (SB203580). PPT induced depolarization of the mitochondrial inner membrane and caspase-dependent apoptosis, which was attenuated by exposure to Z-VAD-FMK. Overall, these data indicate that PPT induced G1 arrest and apoptosis via ROS generation and activation of the p38 MAPK signaling pathway.
Seo, Young Hye,Jeon, Ju-Hyun,Jeong, Miran,Ryu, Seung Mok,Jeon, Won Kyung,Jang, Dae Sik,Shim, Sang Hee,Lee, Dongho,Choi, Jung-Hye,Lee, Jun American Chemical Society and American Society of 2018 Journal of natural products Vol.81 No.7
<P><I>Apios americana</I> is an important food crop producing edible tubers with high nutritional and medicinal values and is widely cultivated in many countries. Despite its usefulness, research on its secondary metabolites and biological activities has been limited. In the present study, a new coumaronochromone, (2<I>R</I>,3<I>S</I>)-3,7,4′-trihydroxy-5-methoxycoumaronochromone (<B>1</B>), and two new isoflavone glucosides, 7,2′,4′-trihydroxy-5-methoxyisoflavone-4′-<I>O</I>-β-<SMALL>D</SMALL>-glucopyranoside (<B>3</B>) and 5,7,4′-trihydroxyisoflavone-7-<I>O</I>-β-<SMALL>D</SMALL>-gentiotrioside (<B>5</B>), were isolated from the tubers of <I>A. americana</I> via chromatographic separation. Seventeen known compounds (<B>2</B>, <B>4</B>, and <B>6</B>-<B>20</B>) were also obtained from this plant part. The chemical structures of <B>1</B>, <B>3</B>, and <B>5</B> were determined by the interpretation of spectroscopic data. The absolute structure of the new compound <B>1</B> was established from experimental and calculated electronic circular dichroism spectra. This is the first study to determine the absolute configuration of a 3-hydroxycoumaronochromone derivative. The potential anti-inflammatory activity of the 20 isolates obtained was evaluated by measuring their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophages. Among the isolates, seven compounds (<B>1</B>, <B>3</B>, <B>6</B>-<B>8</B>, <B>15</B>, and <B>20</B>) showed substantial inhibition of nitric oxide production in RAW 264.7 cells, with the most active being compound <B>1</B> (IC<SUB>50</SUB> value of 0.38 ± 0.04 μM).</P> [FIG OMISSION]</BR>
Management of Focal Chondral Lesion in the Knee Joint
( Seung Suk Seo ),( Chang Wan Kim ),( Dae Won Jung ) 대한슬관절학회 2011 대한슬관절학회지 Vol.23 No.4
Articular cartilage does not contain vascular, nervous and lymphatic tissue and chondrocytes hardly participate in the healing or repair process of chondral tissue because of being surrounded by plenty of extracellular matrix. Therefore, the injury to articular cartilage frequently requires an operative treatment. The goal of surgical repair of articular cartilage is to regenerate nearly normal chondral tissue and prevent degenerative arthritis caused by the articular cartilage defect. Microfracture is a kind of cartilage repair procedure that makes a fibrin clot containing mesenchymal stem cells in the chondral lesion. Microfracture is a simple procedure but it has a disadvantage that the repaired tissue is fibrocartilage. Autologous chondrocyte implantation has an advantage that it implants fully diff erentiated chondrocytes to the lesion, which theoretically produces hyaline cartilage. Its disadvantages are that it is a two stage and a costly procedure. Osteochondral autograft transplantation is a one stage procedure and repairs the lesion with hyaline cartilage. But its limitation is the lack of donor site availability. Surgeons who understand the theoretical background, indications, surgical methods, rehabilitation, complications, and clinical course of cartilage repair procedures can achieve the goal of preventing degenerative arthritis.