ERK1/2-Mediated Schwann Cell Proliferation in the Regenerating Sciatic Nerve by Treadmill Training

Seo, Tae Beom,Oh, Myung-Jin,You, Byoung-Gun,Kwon, Ku-Birm,Chang, In-Ae,Yoon, Jin-Hwan,Lee, Chan-Yong,Namgung, Uk Mary Ann Liebert 2009 JOURNAL OF NEUROTRAUMA - Vol.26 No.10

<P>Proliferation of Schwann cells in the injured peripheral nerve supports axonal regeneration, and physical training in experimental animals has been shown to promote nerve regeneration. Extracellular signal-regulated kinase 1/2 (ERK1/2) activity can mediate neuronal responses to lesion signals, but its role in non-neuronal cells in the injured area is largely unknown. Here we report that treadmill training (TMT) facilitates axonal regeneration via the upregulation of phospho-ERK1/2 protein levels in Schwann cells in the injured sciatic nerve. Low-intensity, but not high-intensity, TMT increased neurite outgrowth of dorsal root ganglion (DRG) sensory neurons and potentiated Schwann cell proliferation. TMT elevated levels of GAP-43 mRNA and protein, and phospho-ERK1/2 protein in the injured sciatic nerves. TMT also enhanced phospho-c-Jun protein levels in the injured nerve. In-vivo administration of the ERK1/2 inhibitor PD98059 eliminated phospho-c-Jun, suggesting ERK1/2 phosphorylation of the c-Jun protein. PD98059 treatment decreased levels of BrdU-labeled proliferating Schwann cells in the distal portion of the injured nerve, and delayed the axonal regrowth that was promoted by TMT. The present data suggest that increased ERK1/2 activity in Schwann cells may play an important role in TMT-mediated enhancement of axonal regeneration in the injured peripheral nerve.</P>

울릉도 소의 전염성 병원체 감염률 조사

서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),우인옥 ( In Ohk Ouh ),조민희 ( Min Hee Cho ),김중규 ( Joong Kew Kim ),김영환 ( Young Hoan Kim ),박노찬 ( No Chan Park ),곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4

Prevalence of infectious agents, including Brucella abortus (BA), Mycobacterium bovis (MB), bovine leukemia virus (BLV), M. avium subsp. paratuberculosis (MP), Neospora caninum (NC) and Toxoplasma gondii (TG), was investigated in all the cattle raised in Ulleung island during 2007∼ 2010. For BA, the prevalences in head and farm were 8.1% (44/545) and 5.5% (4/73) in 2007, all negative in 2008∼2009, and 0.5% (4/774) and 1.7% (1/58) in 2010, respectively. For MB, no sample was positive by PPD or ELISA in 2007∼2010. For BLV and MP, no sample was positive by ELISA in 2007∼2009. For NC, seroprevalences in head and farm were 0.2% (1/545) and 1.4% (1/73), respectively, in 2007 and all negative in 2008∼2009. For TG, seroprevalences in head and farm were 17.6% (97/552) and 54.8% (34/62) by ELISA in 2009. By regions, the seroprevalences of TG in Ulleung-eup, Seo-myeon and Buk-myeon were 26.0%, 9.8% and 16.7%, respectively, which had significant differences (P<0.0001). Tiger cattle were more resistant to TG infection than Hanwoo. The seroprevalence of TG in summer was higher than in autumn. The seroprevalence of TG in cows was higher than in oxen. The seroprevalence of TG in cattle was increased with age. In conclusion, this study indicates that the prevalences of six infectious diseases, except for TG which are widely spread, are relatively low in cattle reared in Ulleung island.

五加皮의 造血 및 免疫調節效果에 대한 實驗的 硏究

任中根,徐榮培,金東熙,薛仁燦 대한본초학회 2000 大韓本草學會誌 Vol.15 No.1

In order to investigate the reinforcemental Effects of Acanthopanax Radicis Cortex about immunity and hematogenic action. The various immune responses were studied. The results were obtained as follows: 1. Positive cell was significantly increased at the 0.25 d r n P degree of density in the effect of CD4 about the spleen cell. 2. In the effect of IgG positive cell was significantly increased as comrared with the data of control only at the 0.25 mg/ml degree of density. 3. In the effect of Mac-1 positive cell was significantly increased in all treated degree of density. 4. In result of observing the change of WBC after Cyclophosphamide-processing the number of WBC was significantly increased in treated Group. 5. Measurement of RT-PCR in Hematopoietic stem cell TPO PCR products was increased as compared with medium at the 1 ㎛/ml degree of density. 6. In reinforcemental effect about Stem cell factor SCF PCR product was increased as compared with medium at the low degree of density. 7. In reinforcemental effect about IL-3 PCR product was increased as compared with medium at the low degree of density. 8. In reinforcemental effect about IL-6 PCR product was significantly increased as compared with all treated group. 9. Assay about positive cell in the spleen cell, the positive cell was significantly increased in CD4, CD19. 10. Created amount NO was treated group significantly increased all degree of density. 11. In promotional dissolution-effect, treated group was increased one and half times or twice all degree of density as compared with negative control. According to the above results, Acanthopanax Radicis Cortex increase coherence with T-cell by dint of activating CD of T-cell, presentate an antigen, activate IL-3, IL-6 which stimulate B-cell mading an antibody, activate IFN- y which appeal anti-viral activity by dint of activating macrophage, activate IgG which being made in B-cell neutralize Virus directly, increase immunity by means of promoting dissolution of Spleen cell which involve immune cell and strengthen hematogenic action by dint of activating TPO, SCF. Although Acanthopanax Radicis Cortex was used in retrogressional joint disease of deficiency syndrome by reinforcing Liver and Kidney, strengthening bones and sinews, because IFN- 7 which appeal anti-viral activity and IL-6 which almost appear in arthritis increased significantly, in future we should make an effort in looking into action of Acanthopanax Radicis Cortex about common cold by infection and arthritis of excess syndrome.

하지 절단자 보행의 운동역학적 분석

임규찬,박윤서,배성제,정철수,신인식,김복영 師範大學 體育硏究所 1994 서울大學校 體育硏究所論集 Vol.15 No.2

This study was conducted to investigate the biomechanical characteristics of the gait pattern in an amputee for the purpose of improving the proper gait posture and designing the prosthetic. The subjects were six males amputees and one normal person. The ground reaction force was measured using AMTI force platform. The conclusions were as follows; 1. In case of amputee group, the ground reaction force was revealed in specific figure according to position in which they used the prosthetic, whereas in case of normal person the ground reaction force was revealed in the figure of two humps in regard of vertical axis. 2. In case of amputee group, the ground reaction force in time between fight foot contact and left foot contact was deviated from a figure of rectangle, whereas in case of normal person the ground reaction force was revealed in the figure of rectangle. 3. In case of amputee group, the ground reaction force referred to both feet angle point of view was revealed in irregular figure, whereas in case of normal person the ground reaction force was revealed about 47。

Modeling of asymmetric degradation based on a?non-uniform electric field and temperature in amorphous In–Ga–Zn–O thin film transistors

Kim, Jong In,Jeong, Chan-Yong,Kwon, Hyuck-In,Jung, Keum Dong,Park, Mun Soo,Kim, Ki Hwan,Seo, Mi Seon,Lee, Jong-Ho Institute of Physics 2017 Semiconductor science and technology Vol.32 No.3

<P>We propose a new local degradation model based on a non-uniform increase in donor-like traps (DLTs) determined by distributions of an electric field and?measured device temperature in amorphous In–Ga–Zn–O (a-IGZO) thin film transistors (TFTs). A systematic investigation of the degradation model reveals that vertical field-dependent DLTs are essential for modeling of measured asymmetric electrical characteristics between the?source and drain after positive gate and drain bias stressing. An increased temperature due to self-heating is found to play a role in intensifying the asymmetric degradation. From the individual simulation of measured transfer curves at different stress times, the?model parameters and an asymmetry index as a function of stress time are extracted. It is expected that this novel methodology will?provide new insight into asymmetric degradation and?be utilized to predict the influence of electric field and heat on?degradation under various bias-stress conditions in a-IGZO TFTs.</P>

근관 세척법이 Resilon/Epiphany sealer와 Gutta-percha/AH 26 sealer의 근관 상아질과의 접착에 미치는 영향

김서경,황윤찬,황인남,오원만 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.2

본 연구에서는 근관 세척법이 두 가지 근관 충전재 (Gutta-percha/AH 26 sealer, Resilon/Epiphany sealer)의 근관 상아질과의 접착메 미치는 영향을 평가하였다. 총 30개의 발거된 상악 전치를 각자 5개씩 6개의 군으로 분류하여 근관 성형을 하고 마지막 세척을 다음과 같이 시행 한 후 Gl, G2, G3군은 Gutta-percha/AH 26 sealer로, Rl, R2, R3군은 Resilon/Epiphany sealer로 근관 충전하였다. Gl, Rl군 : 5.25% NaOCl세척 G2, R2군 : 5.25% NaOCl세척 후 sterile saline 세척 G3, R3군 : 5.25% NaOCl세척 후 17% EDTA, sterile saline 순으로 세척 각 군의 충전된 치아를 아크릴 레진에 매몰하고 약 1 mm 두께로 절단한 후 만능 물성 시험기상에서 push-out bond strength를 측정하였고 실험 후 파절 양상을 Image-analyzing microscope으로 관찰하여 다음과 같을 결과를 얻었다. 1. Gutta-percha/AH 26 sealer 충전군에서 Resilon/Epiphany sealer 충전군보다 push-out bond strength가 높게 나타났다 (p < 0.05). 2. Resilon/Epiphany sealer 충전군 중 17% EDTA, sterile saline순으로 세척시 5.25% NaOCl 세척에 비해 push-out bond strength가 높게 나타났다 (p<0.05). 3.파절 양상을 분석시 모든 군은 접착성과 혼합성 파절 양상을 나타내었고, Gl, G2, Rl 군은 응집성 파절 양상이 나타나지 않았으며, Resilon/Epiphany sealer 충전군은 Gutta-percha/AH 26 sealer 충전군에 비해 응집성과 혼합성 파절 망상이 더 높은 비율로 나타났다. The purpose of this study was to evaluate whether intracanal irrigation method could affect the adhesion between intracanal dentin and root canal filling materials (Gutta-percha/AH 26 sealer and Resilon/Epiphany sealer). Thirty extracted human incisor teeth were prepared. Canals were irrigated with three different irrigation methods as a final rinse and obturated with two different canal filling materials (G groups : Gutta-percha/AH 26 sealer, R groups : Resilon/Epiphany sealer) respectively. Group Gl, Rl - irrigated with 5.25% NaOCl Group G2, R2 - irrigated with 5.25% NaOCl, sterile saline Group G3, R3 - irrigated with 5.25% NaOCl, 17% EDTA, stehle saline Thirty obturated roots were horizontally sliced and push-out bond strength test was performed in the universal testing machine. After test, the failure patterns of the specimens were observed using Image-analyzing microscope. The results were as follows. 1. Gutta-percha/AH 26 sealer groups had significantly higher push-out bond strength compared with the Resilon/Epiphany sealer groups (p < 0.05). 2. Push-out bond strength was higher when using 17% EDTA followed by sterile saline than using NaOCl as a final irrigation solution in the Resilon/Epiphany sealer groups (p < 0.05). 3. In the failure pattern analysis, there was no cohesive failure in Group Gl, G2, and Rl. Gutta-percha/AH 26 sealer groups appeared to exhibit predominantly adhesive and mixed failure patterns, whereas Resilon/Epiphany sealer groups exhibited mixed failures with the cohesive failure occurred within the Resilon substrate.

2개월 남아에서 발병한 Malignant Ectomesenchymoma

황지영,서지현,김점수,김용숙,이수진,박찬후,김유경,박선후,이정희,안인옥,정기현,박병규 대한소아과학회 2001 小兒科 Vol.44 No.8

근관 충전재 및 치근단 역충전재의 방사선 불투과성에 관한 비교 연구

김용상,김서경,황윤찬,황인남,오원만 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.2

This study was performed to assess the radiopacity of a variety of canal filling and retrograde root-end filling materials according to the specification concerning root canal obturation materials. Ten materials including Gutta-percha pellets, amalgam, Fuji II LC, Dyract^(ⓡ) AP, Super EBA^(ⓡ), IRM^(ⓡ) AH 26^(ⓡ), Sealapex™, Tubli-Seal™ and dentin were evaluated in this study In the first part, densitometric reading of an each step of aluminum step wedge on occlusal film were performed at 60 kVp (0.2, 0.3, 0.4 s), 70 kVp (0.2, 0.3, 0.33 s) to decide appropriate voltage and exposure time. In the second part, ten specimens which are 5 ㎜ in diameter and 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 ㎜ in thickness, were fabricated from each material studied. The specimens were radiographed simultaneously with an aluminum step wedge under decided condition (60 kVp, 0.2 s). The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (㎜ Al). The following results were obtained. 1. Among the various conditions including 0.2 s, 0.3 s, 0.4 s at 60 kVp and 0.2 s, 0.3 s, 0.33 s at 70 kVp, the appropriate voltage and exposure time that meet the requirement of density from 0.5 to 2.0 was 0.2 s at 60 kVp. 2. All of the materials in this study had greater radiopacity than the minimun level recommended by ISO No. 4049 standards. 3. Most of the materials had greater radiopacity than 3 ㎜ Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards except for Fuji II LC and Dyract. It suggests that all experimental canal filling and retrograde root-end filling materials have a Sufficient radiopacity that meet the requirement concerning root canal obturation materials except for Fuji II LC and Dyract. 본 실험에서는 다양한 방사선 노출 조건에 따른 aluminum step wedge에 대한 광학 밀도를 알아보고, 그 중 적절한 노출조건을 선택하여 수종의 근관 충전재와 치근단 역충전재의 방사선 불투과성 정도를 굉가, 비교하였다. 방사선 불투과성의 기준을 위해 11개의 step으로 구성된 aluminum step wedge를 제작하여 , 60 kVp, 70 kVp 관전압 상태에서 각각 0.2. 0.3, 0.4초와 0.2, 0.3, 0.33초의 노출시간으로 교합 필름상에서 방사선 촬영 후 적절한 노출 조건을 구하였다. 직경 5 ㎜,각각의 두께 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 ㎜인 10종 (Gutta-percha, amalgam, Super EBA^(ⓡ), MTA, IRM^(ⓡ), Fuji II LC, Dyract^(ⓡ) AP, AH 26^(ⓡ), Sealapex^(ⓡ), Tubli-Sea™의 근관 충전재와 치근단 역충전재 시편을 각 재료와 두께당 10개씩 제작한 후, 동일한 두께의 상아질 시편 , aluminum step wedge와 함께 정해진 노출조건에 따라 방사선 촬영을 하였다. 모든 필름은 자동현상기로 현상하였다. 시편의 흑화도를 densitometer (Model 07-443, Victoreen Inc, Cleveland, Ohio, USA)로 5회 반복 측정 후, 평균값을 구하여 회귀분석 후 알루미늄 두께로 환산하였다. 얻어진 정보를 분석하여 다음의 결과를 얻었다. 1. 관전압 60 kVp에서 노출시간 0.2, 0.3, 0.4초, 70 kVp에서 0.2, 0.3, 0.33초로 변화를 주어 방사선 촬영을 하였을 때, 흑화도가 0.5 - 2.5 사이여야 한다는 ISO No. 6876 규격에 가장 적합한 것은 60 kVp, 0.2초일 경우였다. 2.측정된 근관 충전재와 치근단 역충전재들의 방사선 불투과성 모두 ISO No. 4049 규격에 적합하였다. 3.광중합형 글래스 아이오노머 (Fuji II LC)와 컴포머 (Dyract)를 제외하고는 ANSI/ADA specification (2000) 또는 ISO No. 6876 (2001)규격이 제시한 최소한 3 ㎜ Al 이상의 방사선 불투과성을 지녀야 한다는 규격에 적합하였다. 이상의 결과는 본 실험에 사용된 수종의 근관 충전재와 치근단 역충전재 중 Fuji Ⅱ LC와 Dyract를 제외하고 모두 규격에 적합한 방사선 불투과성을 가지고 있음을 시사한다.

근관 전색재의 방사선 불투과성에 관한 비교연구

김태민,김서경,황인남,황윤찬,강병철,윤숙자,이재서,오원만 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.1

This study was performed to assess the radiopacity of a variety of root canal sealers according to the Specification concerning root canal sealers. Ten materials including Tubli-Seal™, Kerr Pulp Canal Sealer™, AH 26^(R), AH plus^(R), AH plus jet™, Ad seal™, Sealapex™, NOGENOU™, ZOB seal™, Epiphany™, and dentin were evaluated in this study. In the first part. densitometric reading of an each step of aluminum step wedge on occlusal film was performed at different voltage and exposure time. In the second part, ten specimens were radiographed simultaneously with an aluminum step wedges on the occlusal films under decided condition. The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (mm Al). The following results were obtained 1 Among the various conditions, the appropriate voltage and exposure time that meet the requirement density was 60 kVp at 0.2 s 2 All of the materials had greater radiopacity than 3 mm Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards. 3 The radiopacity of materials increased as thickness of materials increased. 4 The mm Al value of each specimen at 1mm in thickness has a significant difference in the statistics. It suggests that root canal sealers have a sufficient radiopacity that meet the requirement. 다양한 근관전색제는 방사선 사진상 주위 해부학적 구조와 구별될 만한 방사선 불투과성을 나타내야 한다 따라서 이런 물질들이 근관에 충전될 때의 방사선 불투과성 정도를 평가해야 할 필요성이 대두되고 있다. 본 실험에서는 다양한 근관전색제들의 방사선 불투과성을 평가하고자, 방사선 노출조건에 따른 aluminium step wedge에 대한 광학 밀도를 알아보고, 그 중 적절한 노출조건을 선택하여 수종의 근관 전색재의 방사선 불투과성 정도를 알루미늄 두께로 환산하여 비교해 보고자 한다. 방사선 불투과성의 기준을 위해 11개의 step으로 구성된 aluminum step wedge을 사용하여, 60kVP, 70kVp관전압 상태에서 각각 0.2, 0.3, 0.4초 그리고 0.2, 0.3, 0.33초의 꼭 노출시간으로 교합필름상에서 방사선 촬영후 적절한 노출 조건을 구하였다. 직경 5mm 각각의 두께 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mm인 10종 (Tubli-Seal™, Kerr pulp Canal Sealer™, AH26^(R), AHplus^(R), AH plus jet starter kit™, Ad seal™, Sealapex™, Nogenol root canal sealer™, ZOB seal™, Epiphany™)의 근관전색재 시편을 각 재료와 두께당 10개씩 제작한 후, 동일한 두께의 상아질 시편, aluminum step wedge와 함께 정해진 노출시간에 따라 방사선 촬영을 하였다. 모든 필름은 자동현상기로 현상하였다. 시편의 방사선 흑화도를 densitometer로 5회 반복 측정 후, 평균값을 구하여 알루미눔 두께로 환산하였다.얻어진 정보를 분석하여 다음의 결과를 얻었다. 1 관전압 60 kVp에서 노출시간 0.2, 0.3, 0.4초, 70 kVp에서 0.2, 0.3, 0.33초로 변화를 주어 방사선 촬영을 하였을 때 흑화도가 ISO No. 6876 규격에 가장 적합한 것은 60 kVP, 0.2초 일 경우였다. 2 측정된 근관 전색제의 방사선 불투과성은 2.29 mm Al (N0GEN0L)로부터 13.69 mm Al (AH Plus jet)까지 다양하게 나타났으나, 모두 ANSI/ADA specification (2000) 또는 ISO No. 6876 (2001) 규격이 제시한 최소한 3mm Al 이상의 방사선 불투과성을 지녀야 한다는 기준에 적합하였다. 3 재료의 두께가 증가할수록 방사선 불투과성은 증가하지만, 정비례하지는 않았다. 4 각 실험재료의 1 mm 두께의 시편에 대한 mm Al값들은 통계적으로 유의한 차이가 있었다. 이상의 결과는 본 실험에 사용된 수종의 근관 전색재는 모든 규격에 적합한 방사선 불투과성을 가지고 있음을 시사한다.

심혈관계 질환으로 관상질환 집중치료실에 입원한 환자들의 혜후 측정을 위한 APACHEⅢ점수 체계의 임상적 유용성

이공섭,이창선,최현주,홍종서,강지향,김도형,이민영,오인균,김병엽,김상억,김학찬,한승혜,이재용,지영우,안경주 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8