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Osteogenic potential of embryonic stem cells in tooth sockets.
Kang, Hyun Ki,Roh, Sangho,Lee, Gabsang,Hong, Seong-Doo,Kang, Hoin,Min, Byung-Moo D.A. Spandidos 2008 International journal of molecular medicine Vol.21 No.5
<P>Embryonic stem cells (ESCs) are established from blastocysts and give rise to various types of cells and tissues. In the present study, we assessed the osteogenic potential of ESCs using in vitro culture conditions and in vivo differentiation in tooth sockets. An ESC-derived embryoid body (EB) was formed and subsequently induced to an osteogenic lineage. The differentiated EB cells exhibited increased expression of various osteogenic markers as determined by real-time PCR analysis. Likewise, the differentiated EB-derived cells had enhanced alkaline phosphatase activity and calcium accumulation, as determined by cytochemical methods. For in vivo transplantation, mixtures of ESCs and hydroxyapatite/ tricalcium phosphate particles or EBs alone were transplanted into female rat tooth sockets. After 12 weeks, we observed formation of osteogenic structure in the tooth sockets without evidence of teratomas. These data suggest that pluripotent ESCs can serve as an alternative source for the reconstruction of craniofacial structures, as well as for further applications.</P>
시금치 분말 및 농축액의 첨가 급여 수준이 난황 내 루테인 함량에 미치는 영향
강근호(Geunho Kang),김상호(Sangho Kim),김지혁(Jihyuk Kim),강환구(Hwanku Kang),김동욱(Dongwook Kim),나재천(Jaecheon Na),서옥석(Oksuk Suh),조수현(Soohyun Cho),성필남(Pilnam Seong),박범영(Beomyoung Park),장애라(Aera Jang),강선문(Sunmoon Ka 韓國家禽學會 2012 韓國家禽學會誌 Vol.39 No.1
This study was conducted to investigate the effect of dietary supplementation levels of spinach powder and extract on performance, egg quality and lutein content in egg yolk. Laying hens were subjected to one of the following treatments; C, basal diet (BD); T1, BD + 1 ppm lutein from spinach powder; T2, BD + 1 ppm lutein powder from spinach extract; T3, BD + 2 ppm lutein powder from spinach extract; T4, BD + 2 ppm lutein from spinach extract. As a result, performance of laying hens was no significant difference among treatments. T4 had a significantly (p<0.05) higher egg yolk color than the other treatments. As from the lutein content in egg yolk, T4 had a significantly (p<0.05) higher content than the other treatments. Finally, these results suggested that the dietary supplementation for lutein-rich egg production, lutein concentration must be higher than a minimum of 2 ppm. Also, dietary with lutein extract from raw materials was appropriate for lutein-rich egg production than dietary with raw materials.
RNA-seq analysis on tetralocular ovary and high seed yields in yellow sarson of Brassica rapa
Mi-Suk Seo,So Youn Won,Sangho Kang,Seong-Han Sohn,Jung Sun Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In Brassica as matter of seedling manner, they have the bilocular ovary and 20~28 seeds per silique after fertilization. Rarely some of B. juncea and yellow sarson (Brassica rapa ssp, tricolaris) have multilocular ovary. In this stdudy, the LP8 (YS-033, CGN06835) is shown tetralocular ovary as well as high seed yields. As microscope study for the different size of immature bud sections and we have known the floral meristem with already four locules in immature buds less size than 1mm of LP8. To identify of determining of tetralocular ovary formation, RNA-seq was carried out on the isolated RNA from less than 1mm and from 1mm of bud size respectively. By contrast tetralocular ovay and bilocular ovary, Chiifu is used. A total of 994 differentially expressed genes(DEGs) are detected in only LP8. Among the DEGs, we identify 18 DEGs in only immature buds of less size than 1mm. The expression patterns of 18 DEGs are validated by real time quantitative PCR and these genes are cloned and the sequence analyzed. At present, 12 candidated gene are analyzed by sequencing and there are detected by large fragment insertion as well as SNPs in sequence comparison to Chiifu. We will perform the genetic transformation of these DEG genes in Arabidopsis for relation between genes and tetralocular ovary. Our results will be helpful in understanding for mechanisms of tetraovular ovary in Brassica rapa.
Kang, Dong-Hee,Jeon, Sangho,Yoo, Hanbyeol,Ishikawa, Takehiko,Okada, Junpei T.,Paradis, Paul-Franç,ois,Lee, Geun Woo American Chemical Society 2014 Crystal Growth & Design Vol.14 No.3
<P>Crystal–liquid interfacial free energy is important to understand in crystal study, for example, nucleation, crystal growth, and vitrification. Here, we report the nanosized nucleus-supercooled liquid interfacial free energy of early and late transition liquid metals using the electrostatic levitation (ESL) technique and classical homogeneous nucleation theory (CNT). For the estimation of the interfacial free energy, we obtained thermophysical parameters of the transition liquid metals (Ti, Fe, Ni, Zr, Nb, Rh, and Hf), such as hypercooling limit (Δ<I>T</I><SUB>hyp</SUB>), specific heat (<I>C</I><SUB><I>p</I></SUB>), total hemispherical emissivity (ε<SUB>T</SUB>), and density (ρ). The estimated interfacial free energies of Ti, Ni, and Zr agreed well with a previous report having similar hypercooling limit and fusion enthalpy, while Fe, Nb, Rh, and Hf show different values from the report. This reflects the importance of accurate measurement of the two quantities. The obtained Turnbull’s coefficients (α) of the liquid metals is higher than 0.45. The interfacial free energy is discussed with configurationally different local order of the crystal and the liquid.</P><P>We report nanosized crystal nucleus-supercooled liquid interfacial free energy of liquid metals, which were obtained using a containerless technique that provides homogeneous nucleation conditions. While Ti, Ni, and Zr show good agreement with a previous report, refractory materials with high melting temperature, Nb, Rh, and Hf, differ from the previous values.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cgdefu/2014/cgdefu.2014.14.issue-3/cg401597p/production/images/medium/cg-2013-01597p_0006.gif'></P>
Kang, Hoin,Roh, Sangho The Society ; Maruzen Co. [distributor] 2011 The Journal of veterinary medical science Vol.73 No.5
<P>The low viability of embryos reconstructed by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic modification errors, and reduction of those errors may improve the viability of SCNT embryos. The present study shows the effect of trichostatin A (TSA), a strong inhibitor of histone deacetylase, on the development of murine SCNT embryos. After enucleation and nuclear injection, reconstructed murine oocytes were activated with or without TSA for 6 hr (TSA-6 hr). After activation, TSA treatment was extended to 3 hr (TSA-9 hr), 5 hr (TSA-11 hr) and 18 hr (TSA-24 hr) during culture. As a result, the SCNT embryos in the TSA-11 hr group showed a remarkably higher blastocyst rate (21.1%) when compared with the nontreated embryos (3.4%), while the concentration of TSA did not significantly affect embryonic development. The expressions of histone deacetylase (HDAC1 and HDAC2) and DNA methylation (DNMT3a and DNMT3b) genes decreased in the TSA-11 hr and TSA-24 hr groups, while there was an increase in the expression of histone acetyltransferase (P300 and CBP), pluripotency (OCT4 and NANOG) and embryonic growth/trophectoderm formation (FGF4)-related genes in the same groups. The expression of CDX2, a critical gene for trophectoderm formation was upregulated only in the TSA-24 hr group. Our results show that TSA treatment during the peri- and postactivation period improves the development of reconstructed murine embryos, and this observation may be explained by enhanced epigenetic modification of somatic cells caused by TSA-induced hyperacetylation, demethylation and upregulation of pluripotency and embryonic growth after SCNT.</P>