Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교

황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.

Ethylenediamine-N,N'-di-α-Butyric Acid의 로디움(Ⅲ) 착물

전무진,한창윤,박장우,최승락,박광하 연세대학교 자연과학연구소 1985 學術論文集 Vol.15 No.-

네자리 리간드인 ethylenediamine--N,N'-di-α-butyric acid(eddb)의 디클로로 로디움(Ⅲ)착물을 합성하고 크로마토그래피법으로 분리하였다. Eddb리간드가 로디움(Ⅲ) 이온에 배위될 때 s-cis와 uns-cis기하 이성체가 형성됨이 관찰되었다. 합성된 착물의 구조는 적외선 분광분석법, 핵자기 공명 및 흡수 스펙트럼으로 확인되었다. Dichloro rhodium(Ⅲ) complex of a flexible tetradentate ligand of ethylenediamine-N,N'-di-α-butyric acid(eddb) has been prepared and separated by the chromatographic method. S-cis and uns-cis geometric isomers have been yielded during the preparation of complex. IR, PMR and electronic absorption spectra have been used to characterize the complex.

유압구동 습동재의 마찰면 형상특징 해석에 관한 연구

전성재,조연상,김동호,박흥식 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.1

It is known that fractal theory has recently been used as a useful in the characterization of surface texture and the understanding of tribological phenomena such as friction, wear and lubrication. The fractal-based method for describing the rubbed surface texture has roused great interest. In this paper, the fractal descriptors was applied to rubbed surface of hydraulic driving material with image processing system in order to describe morphology of the rubbed surface. The results showed that the fractal dimension can be determined by sum of intensity difference of surface pixel. The two step size to get fractal dimension is similar to surface roughness Ra. Fractal dimensions of the rubbed surfaces increase with an increase of applied load. Morphology of the rubbed surface driving in lubricant can be effectively obtained by fractal dimensions.

社會福祉政策의 분석 : with respect to medical security in Korea 醫療保障制度를 中心으로

全相京 東亞大學校 大學院 1983 大學院論文集 Vol.7 No.-

Much emphasis had been laid upon social development since the fourth Five-Year Economic Plan. Along with this move to put emphasis upon social development, we are more concerned with social welfare than ever before. There are two views on social welfare: residual conception and institutional one. The residual conception of welfare lends weight to the conception that social welfare serves mainly such function wherein its activities are perceived as necessary only when those who are served by the "normal" institutional channels are unable to benefit, either through personel failings and exceptional needs or when those "normal" channels fail to perform appropriately. On the other hand, the institutional conception of welfare is as a distinct pattern of activities serving not a safety net to catch the pieces after all else has failed, but as an integral and "normal first line function of modern industrial society." Medical security is one of social welfare policies in functional views. And medical secutity consists of medical care and medical insurance. Medical care is a kind of public assistance which is conducted by the government revenue, while medical insurance is a sort of social insurance which aims the dispersion of social risk and income re-distribution. When we say about medical security from here on, we mean it both medical care and medical insurance. We analyze medical security using Gilbert and Specht's framework of social welfare policy. Their framework are as follows: the bases of eligibility of medical security benefits, the kind of medical security benefits, the mode of financing for medical security benefits and the delivery system of medical security benefits. To improve the relevance of this study, we should conduct field survey to distil useful information from the receipients of this policy, the authorities concerned with this policy and medical centers which deliver medical services to recipients. But due to many constraints, we depend upon only literary survey such as the acts concerned with medical security.

사모주식투자펀드의 역할 : 간접투자자산운용업법을 중심으로 with Emphasis on the Indirect Investment Asset Management Act

전성인 홍익대학교 경제연구소 2004 經濟硏究 Vol.19 No.-

한강 퇴적물에 함유된 오염물질의 존재형태와 이동성에 관한 연구 : 팔당호 퇴적물의 인과 중금속을 중심으로

전상호 江原大學校 附設 環境硏究所 1990 環境硏究 Vol.7 No.-

Fractional composition and mobility of sediment phosphorus and some heavy metals in Lake Paldang are investigated. The fractional scheme for sediment phosphorus was made for four chemi-cally defined phosphorus forms as adsorbed phosphorus, non. apatite in-organic phosphorus (NAI-P), apatite phosphorus and organic phosphorus (residual P) (Hieltjes and Lijklema, 1980). The most abundant fraction of the sediment phosphorus in NAI-P and secondly organic fraction, thirdly abundant apatite fraction. Adsorbed frac-tion is minor part of the total phosphorus. Mobilization of sediment phoshor-us in Lake Paldang is most likely to occur when the pH increases or oxygen depleted in bottom water. The most abundant fraction of the sediment metal is residual fraction in all metals investigated. Labile fraction of the sediment metals are 18.0% of total Cu, 51.5% of total Zn, 45.3% of total Pb, and 18.8% of total Ni respectively. Most of labile fraction consists of carbonate fraction for Cu, reducible frac-tion for Zn, Pb and Ni. Mobilization of Cu is most likely to occur when pH decreasesm and Zn, Pb and Ni when oxygen is depleted.

의대생의 스트레스에 영향을 미치는 요인 분석

전진용,김상아,문동석,강동원,박웅섭 관동대학교 의과학연구소 2000 關東醫大學術誌 Vol.4 No.1

Background : Generally, it is well known that medical students have lots of stress because they have many classes of which the courses are much different from those of non-medical students. So, this study was designed for the purpose of evaluating the influencing factors on medical students' stress. Method : This study was carried out with the data of 69 students in premedical course and 66 students in medical course, who answered the survey by Kwandong Medical College, Department of Preventive Medicine and Public Health in June, 1999, The independent variables are the general characteristics, the characteristics related with study, the state of health, the activity for free time. The amount of stress is measured by Korean-translated BEPSI (Brief Encounter Psychosocial Instrument). Result : The multiple recession and logistic analysis show that the grade influence the significant difference of the stress level of the students in premedical course. The entrance age, average selfstudy time, hobby and religion influence the significant difference of the stress level of the students in medical course. The grade, avercage self-studying time and smoking habits influence the signifiant difference of the stress level of the medical students. Conclusion : This result will be used for the management of the stress level of the medical students' that influences their study.

민영화 시대의 은행 지배구조화 감독정책

전성인 홍익대학교 경제연구소 2001 經濟硏究 Vol.16 No.-

뉴질랜드의 금융개혁과 한국에의 정책적 함의

全聖寅 홍익대학교 경제연구소 1997 經濟硏究 Vol.12 No.-