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Shaban, Nadia Z,Salem, Halima H,Elsadany, Mohamed A,Ali, Bahy A,Hassona, Ehab M,Mogahed, Fayed AK Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4
Background: Infection with hepatitis B virus (HBV) is a major global public health problem, with a wide spectrum of clinical manifestations. Human cytosolic glutathione-S-transferases (GSTs) include several classes such as alpha (A), mu (M), pi (P), sigma (S), zeta (Z), omega (O) and theta (T). The present study aimed to investigate the role of GST omega genes (GSTO1 and GSTO2) in different groups of patients infected with HBV. Materials and Methods: HBV groups were classified according to clinical history, serological tests and histological analysis into normal carriers (N), acute (A), chronic (CH), cirrhosis (CI) and hepatocellular carcinoma (HCC) cases. The study focused on determination of the genotypes of GST omega genes (GSTO1 and GSTO2) and GST activity and liver function tests. Results: The results showed that GSTO1 (A/A) was decreased in N, A, CH, CI and HCC groups compared to the C-group, while, GSTO1 (C/A) and GSTO1(C/C) genotypes were increased significantly in N, A, CH, CI and HCC groups. GSTO2 (A/A) was decreased in all studied groups as compared to the C-group but GSTO2(A/G) and GSTO2(G/G) genotypes were increased significantly. In addition, GST activities, albumin and TP levels were decreased in all studied groups compared to the C-group, while the activities of transaminases were increased to differing degrees. Conclusions: The results indicate that GSTO genetic polymorphisms may be considered as biomarkers for determining and predicting the progression of HBV infection.
El-Shiekh Riham A.,Elshimy Rana,Mandour Asmaa A.,Kassem Hanaa A. H.,Khaleel Amal E.,Alseekh Saleh,Fernie Alisdair R.,Salem Mohamed A. 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-
Acinetobacter baumannii is without a doubt one of the most problematic bacteria causing hospital-acquired nosocomial infections in today’s healthcare system. To solve the high prevalence of multi-drug resistant (MDR) in A. baumannii, we investigated one of the medicinal plants traditionally used as antibacterial agent; namely Murraya koenigii (L.) Sprengel. The total methanolic extracts of seeds and pericarps were prepared and their anti-bacterial activity was assessed using the agar diffusion method and minimum inhibitory concentration (MIC) was then calculated as compared to tigecycline. Then, an in-vivo murine model was established which confirmed the promising activity of M. koenigii seeds in demonstrating anti-bacterial and anti-inflammatory actions. The histopathological study of lungs, scoring of pulmonary lesions, counting of bacterial loads after infection by multi-drug resistant A. baumannii all provided evidence to support these findings. LC–MS/MS profiling coupled to molecular networking and chemometrics detected the presence of carbazole alkaloids, and coumarins as dominate metabolites of the active seed extracts. Positively correlated metabolites to antibacterial potential were 6-(2ʹ,3ʹ-dihydroxy-3-methylbutyl)- 8-prenylumbelliferone, scopoline, and 5-methoxymurrayatin. An in-silico study was also performed on the crystal structure of MurF from A. baumannii (PDB ID: 4QF5), the studied structures of the mentioned extracts revealed good docking interaction at the active site suggestive of competition with the ATP ligand. These collective findings suggest that extracts of Murraya koenigii (L.) Sprengel seed is a novel prospective for the discovery of drug candidates against infections caused by MDR A. baumannii.
Mohammed Nabil A.,Afzal Muhammad,Al-Faifi Sulieman A.,Khan Muhammad A.,Refay Yahya A.,AL-Samin Bazel H.,Alghamdi Salem S.,Ibrahim Abdullah 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4
Lentil is an important annual food legume crop, nitrogen fixer and provides a substantial amount of protein, carbohydrate, minerals, and vitamin content. The use of molecular markers to assess genetic diversity is crucial for crop improvement, efficient management, and conservation of plant genetic resources. The current study aimed to determine the genetic diversity among lentil genotypes using sequence-related amplified polymorphism (SRAP) markers. Therefore, we evaluated a collection of 36 lentil genotypes, including 20 from Yemen, Saudi Arabia (7), Egypt (4), and Bangladesh (3), and (2) genotypes from the International Center for Research in Dry Area (ICARDA) using 21 SRAP primer combinations. The amplified fragments showed a high level of useful polymorphic amplified fragments (775 out of 782) indicating a higher degree of variation. The polymorphic information content (PIC) ranged from 0.31 to 0.39 with an average of 0.33 for each primer. The UPGMA trees, based on Jaccard similarity index matrices, separated the genotypes into four main clusters according to their geographical origin. The population structure supported the major groups and attested to their great degree of differentiation. The highest lentil population was found at K = 3, K = 5, and K = 7 levels, showing purity and admixture ancestry among the lentil population. This study highlighted the wide genetic diversity among the studied lentil genotypes and demonstrated the effectiveness of the SRAP technique in determining the genetic variability of lentil. Furthermore, it could be used to establish the genetic peculiarity of ecotypes when applying for the obtainment of origin and agro-morphological characteristics.
Mohamed Safaa H.,Shahat Abdelaaty A.,Mohamed Mohamed Ragaa,Khalil Wagdy K. B.,Salem Ahmed M.,Farrag Abdel Razik H.,Ahmed Hanaa Hamdy 한국약제학회 2021 Journal of Pharmaceutical Investigation Vol.51 No.2
Purpose Our research aims to address and determine the effect of Camellia sinensis extract in the management of nonalcoholic steatohepatitis (NASH) in rats. Methods Forty adult female albino rats were divided into four groups. Group 1 (G1) served as the control group, while the other three groups received high-fat diet for 32 weeks to induce NASH and then were later assigned to the following groups: (G2) NASH-afflicted group which was left untreated, (G3) NASH-afflicted group treated with Camellia sinensis extract in a dose of 400 mg/kg, and (G4) NASH-afflicted group treated with Camellia sinensis extract in a dose of 200 mg/kg. Results Significant elevation in serum alanine aminotransferase, albumin, bilirubin (total and direct), cholesterol, low density lipoprotein, triglycerides, leptin, Cox-2, and CD40 values was recorded. Moreover, overexpression of hepatic tumor necrosis factor alpha and hepatocyte growth factor genes were recorded, whereas blood platelet count and serum high density lipoprotein concentration revealed significant depletion, which was paralleled by significant downregulation of hepatic adiponectin gene expression level in NASH group versus the control group. On the opposite side, treatment of NASH groups with two different doses of Camellia sinensis extract reversed the values of the measured biochemical parameters and the targeted gene expression levels when compared with the NASH group. Optical micrograph of liver tissue sections of rats treated with Camellia sinensis extract showed the observed improvement in the studied biochemical and genetic markers. Conclusion This study provides a clear evidence for the promising therapeutic potential of Camellia sinensis extract against NASH. This could be ascribed to its hepatoprotective activity, hypolipidemic effect, and anti-inflammatory potency.
Hibiscus sabdariffa L.: phytoconstituents, nutritive, and pharmacological applications
Mohamed A. Salem,Ahmed Zayed,Magy E. Beshay,Mesih Mirna M. Abdel,Khayal Reem F. Ben,Fady A. George,Shahira M. Ezzat 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.3
Hibiscus sabdariffa L. (HS) calyx extracts have been well-documented for the treatment of hypertension, liver dysfunctions, and diabetes among others. An updated concise review of HS regarding phytoconstituents, and involved putative mechanisms of potential biological activities is presented. HS showed other food and other industrial applications, including the preparation of metallic nanoparticles. These activities were explained by the presence of a broad spectrum of valuable phytochemicals, including the red pigments anthocyanins, phenolic, and organic acids and polyphenolics (e.g., flavonoids and tannins). Most of the bioactivities were found to be associated with anthocyanins-rich extracts. Anthocyanins were found to have various mechanisms for the treatment of hypertension, including direct vasodilation and inhibition of angiotensinconverting enzyme (ACE). However, leaves and seeds showed also other potential applications in food industry, owing to the significant amounts of phenolic antioxidant compounds. Therefore, valorization and optimization of bioactive constituents’ extraction from plant biowastes should be considered for maximizing the benefits of HS extracts.
A.H. Alamoodi,Mohammed Rashad Baker,O.S. Albahri,B.B. Zaidan,A.A. Zaidan,Wing-Kwong Wong,Salem Garfan,A.S. Albahri,Miguel A. Alonso,Ali Najm Jasim,M. J. Baqer 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.7
The COVID-19 pandemic has affected many aspects of human life. The pandemic not only caused millions of fatalities and problems but also changed public sentiment and behavior. Owing to the magnitude of this pandemic, governments worldwide adopted full lockdown measures that attracted much discussion on social media platforms. To investigate the effects of these lockdown measures, this study performed sentiment analysis and latent Dirichlet allocation topic modeling on textual data from Twitter published during the three lockdown waves in Malaysia between 2020 and 2021. Three lockdown measures were identified, the related data for the first two weeks of each lockdown were collected and analysed to understand the public sentiment. The changes between these lockdowns were identified, and the latent topics were highlighted. Most of the public sentiment focused on the first lockdown as reflected in the large number of latent topics generated during this period. The overall sentiment for each lockdown was mostly positive, followed by neutral and then negative. Topic modelling results identified staying at home, quarantine and lockdown as the main aspects of discussion for the first lockdown, whilst importance of health measures and government efforts were the main aspects for the second and third lockdowns. Governments may utilise these findings to understand public sentiment and to formulate precautionary measures that can assure the safety of their citizens and tend to their most pressing problems. These results also highlight the importance of positive messaging during difficult times, establishing digital interventions and formulating new policies to improve the reaction of the public to emergency situations.
Middle Meningeal Artery Arising from the Basilar Artery
Mohamed M. Salem,Matthew R. Fusco,Parviz Dolati,Arra S. Reddy,Bradley A. Gross,Christopher S. Ogilvy,Ajith J. Thomas 대한뇌혈관외과학회 2014 Journal of Cerebrovascular and Endovascular Neuros Vol.16 No.4
Various anomalies for the origin of the middle meningeal artery (MMA)have been described in the literature. However, origin of the MMA fromthe basilar trunk is an extremely rare variant. We report on a 54-year-oldfemale who presented with frequent headaches; magnetic resonanceimaging showed a right parietal meningioma. The abnormal origin of themiddle meningeal artery from the basilar artery was diagnosed by angiographyperformed for preoperative embolization of the tumor. We reporton the case with a review of the embryologic basis, possible explanationsfor this aberrant origin, and its clinical implications.
Mohamed A. Hassan,Tatiq R. Sobahi,Ali Kh. Khalil,Salem A. Basaif 대한화학회 2005 Bulletin of the Korean Chemical Society Vol.26 No.11
Aldol condensation of 2-acetylthiophene, 2-acetylpyrrole and 2-acetylpyridine with different aromatic aldehydes were carried out in water in heterogeneous phases in the presence of cetyltrimethylammonium bromide as cationic surfactant at room temperature. All the reactions occur in a short time with excellent yields of steroselective hetarylpropanones in water as environmental friendly solvent.
Basaif, Salem A.,Sobahi, Tatiq R.,Khalil, Ali Kh.,Hassan, Mohamed A. Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.11
Aldol condensation of 2-acetylthiophene, 2-acetylpyrrole and 2-acetylpyridine with different aromatic aldehydes were carried out in water in heterogeneous phases in the presence of cetyltrimethylammonium bromide as cationic surfactant at room temperature. All the reactions occur in a short time with excellent yields of steroselective hetarylpropanones in water as environmental friendly solvent.
MicroRNAs and Metastasis-related Gene Expression in Egyptian Breast Cancer Patients
Hafez, Mohamed M.,Hassan, Zeinab K.,Zekri, Abdel Rahman N.,Gaber, Ayman A.,Rejaie, Salem S. Al,Sayed-Ahmed, Mohamed M.,Shabanah, Othman Al Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2
Aim and background: MicroRNAs (miRNAs) are a class of naturally occurring small noncoding RNAs that regulate gene expression, cell growth, differentiation and apoptosis by targeting mRNAs for translational repression or cleavage. The present study was conducted to study miRNAs in Egyptian breast cancer (BC) and their relation to metastasis, tumor invasion and apoptosis in addition to their association with the ER and PR statuses. Methods: Real Time RT-PCR was performed to identify the miRNA expression level of eight miRNAs and eight metastatic-related genes in 40 breast cancer samples and their adjacent non-neoplastic tissues. The expression levels of each miRNA relative to U6 RNA were determined using the $^{2-{\Delta}}CT$ method. Also, miRNA expression profiles of the BC and their corresponding ANT were evaluated. Results: The BC patients showed an up-regulation in miRNAs (mir-155, mir-10, mir-21 and mir-373) with an upregulation in MMP2, MMp9 and VEGF genes. We found down regulation in mir-17p, mir-126, mir-335, mir-30b and also TIMP3, TMP1 and PDCD4 genes in the cancer tissue compared to the adjacent non-neoplastic tissues. Mir -10b, mir -21, mir-155 and mir373 and the metastatic genes MMP2, MMP9 and VEGF were significantly associated with an increase in tumor size (P < 0.05). No significant difference was observed between any of the studied miRNAs regarding lymph node metastasis. Mir-21 was significantly over-expressed in ER-/PR-cases. Conclusion: Specific miRNAs (mir-10, mir-21, mir-155, mir-373, mir-30b, mir-126, mir-17p, mir-335) are associated with tumor metastasis and other clinical characteristics for BC, facilitating identification of individuals who are at risk.