Evaluating the effect of conditioned medium from endometrial stem cells on endometriosis-derived endometrial stem cells

Seyedeh Saeideh Sahraei,Ali Kowsari,Faezeh Davoodi Asl,Mohsen Sheykhhasan,Leila Naserpoor,Azar Sheikholeslami 대한해부학회 2022 Anatomy & Cell Biology Vol.55 No.1

Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium (CM) from menstrual blood-derived mesenchymal stem cells (MenSCs) of healthy women can affect the expression level of inf lammatory and stemness genes of MenSCs from endometriosis women. Endometriosis-derived MenSCs (E-MenSCs) were treated with CM derived from healthy women’s MenSCs (non-endometriosis derived MenSCs [NE-MenSCs]). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the interleukin-1, cyclooxygenase-2, and hypoxia-inducible factor 1α as inflamaturay genes and octamer-binding transcription factor 4, NANOG, and sex determining region Y-box 2 as stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Our study indicates that the expression level of some inflammatory- and stemness-related genes which have differential expression in E-MenSCs compared with NE-MenSCs, could be changed to normal status by using CM derived from NE-MenSCs.

Effects of Topical Tamoxifen on Wound Healing of Burned Skin in Rats

Mehrvarz, Shaban,Ebrahimi, Ali,Sahraei, Hedayat,Bagheri, Mohammad Hasan,Fazili, Sima,Manoochehry, Shahram,Rasouli, Hamid Reza Korean Society of Plastic and Reconstructive Surge 2017 Archives of Plastic Surgery Vol.44 No.5

Background This study aimed to assess the effects of the topical application of tamoxifen on wound healing of burned skin in Wistar rats by evaluating 3 healing characteristics: fibrotic tissue thickness (FTT), scar surface area (SSA), and angiogenesis in the healed scar tissue. Methods Eighteen male Wistar rats were used in this study. A third-degree burn wound was made on the shaved animals' back, measuring $2{\times}2{\times}2cm$. In the first group, a 2% tamoxifen ointment was applied to the wound twice daily for 8 weeks. The second group received a placebo ointment during the same period. The third group did not receive any treatment and served as the control group. Results The median (interquartile range=[Q1, Q3]) FTT was 1.35 (1.15, 1.62) mm, 1.00 (0.95, 1.02) mm, and 1.25 (0.8, 1.5) mm in the control, tamoxifen, and placebo groups, respectively (P=0.069). However, the FTT in the tamoxifen group was less than in the placebo and control groups. The median angiogenesis was 3.5 (3.00, 6.25), 8.00 (6.75, 9.25), and 7.00 (5.50, 8.25) vessels per high-power field for the control, tamoxifen, and placebo groups, respectively (P=0.067). However, the median angiogenesis was higher in the tamoxifen group than in the control group. No significant difference was observed in the mean SSA between the tamoxifen group and the control group (P=0.990). Conclusions Local application of tamoxifen increased angiogenesis and decreased the FTT, with no change in the SSA in burned skin areas. These effects are expected to expedite the wound healing process, reducing contracture and preventing hypertrophic scar and keloid formation.

Effects of Electromagnetic Radiation Exposure on Stress- Related Behaviors and Stress Hormones in Male Wistar Rats

( Seyed Mohammad Mahdavi ),( Hedayat Sahraei ),( Parichehreh Yaghmaei1 ),( Hassan Tavakoli ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6

Studies have demonstrated that electromagnetic waves, as the one of the most important physical factors, may alter cognitive and non-cognitive behaviors, depending on the frequency and energy. Moreover, non-ionizing radiation of low energy waves e.g. very low frequency waves could alter this phenomenon via alterations in neurotransmitters and neurohormones. In this study, short, medium, and long-term exposure to the extremely low frequency electromagnetic field (ELF-EMF) (1 and 5 Hz radiation) on behavioral, hormonal, and metabolic changes in male Wistar rats (250 g) were studied. In addition, changes in plasma concentrations for two main stress hormones, noradrenaline and adrenocorticotropic hormone (ACTH) were evaluated. ELF-EMF exposure did not alter body weight, and food and water intake. Plasma glucose level was increased and decreased in the groups which exposed to the 5 and 1Hz wave, respectively. Plasma ACTH concentration increased in both using frequencies, whereas noradrenaline concentration showed overall reduction. At last, numbers of rearing, sniffing, locomotor activity was increased in group receiving 5 Hz wave over the time. In conclusions, these data showed that the effects of 1 and 5 Hz on the hormonal, metabolic and stress-like behaviors may be different. Moreover, the influence of waves on stress system is depending on time of exposure.

Silver nanoparticle-based spectrophotometric method for quantification of nile blue A in river water

S.S. Mortazavi,R. Sahraei,A. Farmany 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4

A novel and sensitive spectrophotometric method is developed for the determination of nanomolar level of nile blue A in water samples based on its catalytic effect of silver nanoparticles on the oxidation of nile blue A by hexacyanoferrate (III) in acetate-acetic acid medium and at 25 ℃. The absorbance is measured at 595.5 nm with the fixed-time method. The optimization of the operating conditions regarding concentration of the reagents, temperature and interferences is also investigated. The calibration curve is linear over the concentration range between 85 and 2000 nM of nile blue A with good precision and accuracy. The detection limit of the method is down to 54. The relative standard deviation for a standard solution of 100.0 nM of nile blue A is 1.63% (n = 10). The proposed method provides a highly sensitive, selective and relatively rapid assay for nile blue A at nanomolar level without any pre-concentration and separation step. The method was applied to the determination of nile blue A in river water samples.

Effect of Purposive Distribution of Fibers to Prevent the Penetration of Bullet in Concrete Walls

Fereydoon Omidinasab,Amirhosein Sahraei Moghadam 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.3

In this study, 10 different types of walls were made by fiber reinforced self-compacting concrete. The concrete walls were reinforced with steel and nylon fibers. The results of rheological tests showed that the steel and nylon fibers have a negative effect on flowability of self-compacting concrete, which the negative effect of steel fibers was more significant in this regard. On the other hand, the results of mechanical tests indicated that the steel fibers have a slight effect on the compressive strength and considerable effect on tensile and flexural strength, whereas, nylon fibers have no significant effect in these cases. In this study, two methods, including uniform distribution and targeted distribution of fibers, were used to reinforce the concrete walls. In the uniform distribution method, all the used fibers were uniformly distributed in the wall, while in the targeted distribution method the used fibers were distributed in the wall, with a more volume of fibers in the surrounding layers and a fewer volume of fibers in the middle layer of the wall. The concrete walls were tested by bullet fire. The results of this test showed that steel fibers could significantly decrease the penetration depth of bullet in concrete walls, while nylon fibers had a much lower effect. By comparing the penetration depth of the bullet in the walls, it was observed that the targeted distribution of fibers was effective to decrease the penetration depth of the bullet.

Comparison of removal of bromothymol blue from aqueous solution by multiwalled carbon nanotube and Zn(OH)2 nanoparticles loaded on activated carbon: A thermodynamic study

M. Ghaedi,N. Taghavimoghadam,S. Naderi,R. Sahraei,A. Daneshfar 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.5

In this research Zn(OH)2 nanoparticles loaded on activated carbon (Zn(OH)2-NPs-AC) as novel adsorbent and raw multiwalled carbon nanotube (MWCNT) were applied for efficient removal of bromothymol blue (BTB). Both adsorbent has been characterized with different techniques such and SEM, XRD and UV–vis spectrometry. Their size was less than 100 nm. In the removal process the variables are pH,temperature, concentration of BTB, amount of adsorbent and contact time that their influence on removal of BTB was optimized using one at a time approach in batch procedure. Adsorptions of BTB on bath adsorbent depend highly on pH. Following the investigation of temperature effect, the thermodynamic parameters including change in entropy, enthalpy and free Gibbs energy were calculated. For both adsorbents, positive value of enthalpy and negative value of DG0 show routine feasibility of adsorption using energy. At optimum value of variables, the removal processes onto both adsorbent have high adsorption capacity for best fitting model Langmuir, i.e. for Zn(OH)2-NP-AC and 150 mg/g for PAC. The adsorption rates were well explained with pseudo second order and interparticle diffusion model. It is expected that there could an increase in the number of reactive sites due to their expected high volume, pore size and high surface area.

Effects of Topical Tamoxifen on Wound Healing of Burned Skin in Rats

Shaban Mehrvarz,Ali Ebrahimi,Hedayat Sahraei,Mohammad Hasan Bagheri,Sima Fazili,Shahram Manoochehry,Hamid Reza Rasouli 대한성형외과학회 2017 Archives of Plastic Surgery Vol.44 No.5

Background This study aimed to assess the effects of the topical application of tamoxifen on wound healing of burned skin in Wistar rats by evaluating 3 healing characteristics: fibrotic tissue thickness (FTT), scar surface area (SSA), and angiogenesis in the healed scar tissue. Methods Eighteen male Wistar rats were used in this study. A third-degree burn wound was made on the shaved animals’ back, measuring 2×2×2 cm. In the first group, a 2% tamoxifen ointment was applied to the wound twice daily for 8 weeks. The second group received a placebo ointment during the same period. The third group did not receive any treatment and served as the control group. Results The median (interquartile range=[Q1, Q3]) FTT was 1.35 (1.15, 1.62) mm, 1.00 (0.95, 1.02) mm, and 1.25 (0.8, 1.5) mm in the control, tamoxifen, and placebo groups, respectively (P=0.069). However, the FTT in the tamoxifen group was less than in the placebo and control groups. The median angiogenesis was 3.5 (3.00, 6.25), 8.00 (6.75, 9.25), and 7.00 (5.50, 8.25) vessels per high-power field for the control, tamoxifen, and placebo groups, respectively (P=0.067). However, the median angiogenesis was higher in the tamoxifen group than in the control group. No significant difference was observed in the mean SSA between the tamoxifen group and the control group (P=0.990). Conclusions Local application of tamoxifen increased angiogenesis and decreased the FTT, with no change in the SSA in burned skin areas. These effects are expected to expedite the wound healing process, reducing contracture and preventing hypertrophic scar and keloid formation

Combination of alpha lipoic acid and metformin supplement improve assisted reproductive technologies outcomes in polycystic ovary syndrome patients

Rahil Jannatifar,Hamid Piroozmanesh,Seyedeh Saeideh Sahraei,Elham Asa 대한해부학회 2022 Anatomy & Cell Biology Vol.55 No.2

Application of experimental design for removal of sunset yellow by copper sulfide nanoparticles loaded on activated carbon

Saeid Khodadoust,Mehrorang Ghaedi,Reza Sahraei,Ali Daneshfar 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

In this study, copper sulfide nanoparticles loaded on activated carbon (CuS-NP-AC) was synthesized by novel, low cost and green approach and characterized using different techniques such as SEM, and BET. This material was used for the removal of sunset yellow (SY) from aqueous solutions was investigated. The dependency of removal percentages to variables such as pH, initial SY concentration, adsorbent dosage and sonication time were studied by central composite design (CCD) coupled with response surface methodology (RSM) by considering the desirability function (DF). The accuracy and ability of method at optimum values predicted by this model was studied by conduction of similar experiments at the same previously optimized conditions. A good agreement between experimental and predicted data was achieved that efficiency of this model for prediction of real optimum point. Among the well known previously isotherm models, the experimental equilibrium data efficiently can be represented by the Langmuir model, while the rate of adsorption. Kinetic data efficiently can be interpreted by combination of pseudo-second order as well as intraparticle diffusion models. The small amount of this adsorbent (0.016 g) is applicable for removal of high amount of SY (>90%) in reasonable time (17 min).

Comparison of the influence of nanomaterials on response properties of copper selective electrodes

Mehrorang Ghaedi,Morteza Montazerozohori,Reza Sahraei 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.4

Novel Cu2+ ion selective electrode in carbon paste matrices based on incorporation of bis(2-hydroxynaphthaldehydene)-1,6-hexanediamine (BHNHDA) has been developed. The influence of variables including sodium tetraphenylborate (NaTPB), ionophore, and amount of multiwalled carbon nanotubes (MWCNT) and Nujol and effect of two new nanoparticles including gold nanoparticles loaded on activated carbon (Au-NP-AC) and zinc sulfide nanoparticles loaded on activated carbon (ZnS-NP-AC)on the electrodes response was studied and optimized. At optimum specified conditions, the proposed electrodes have appropriate advantages such as short response times and suitable reproducibility and applicability for a period of at least 1 month without any significant divergence in slope and response properties. The sensor based on impregnations of MWCNT, Au-NP-AC and ZnS-NP-AC have wide linear range of concentration (6 x 10-8–1.0 x 10-1 mol L-1) and detection limit of lower than 4 x 10-8 mol L-1of Cu2+ ion. The electrodes based on incorporation of Au-NP-AC and ZnS-NP-AC have Nernstian response with slope of 29.34 ± 1.41 and 29.78 ± 1.23 mV decade-1 and response is independent of pH in the range of 2.0–5.0. Finally, these electrodes have been successfully applied for the determination of Cu2+ ions content in various real samples. Due to their acceptable selectivity coefficient, they are usable for accurate and successful evaluation of Cu2+ ions content in various real sample with complicated matrices.