Trichoderma viride에서 분리한 Endoglucanases와 Cellulose의 상호작용에 있어서 Enzyme에 존재하는 Trytophan기의 역할

원성미,장영훈,김동원,정영규,류해일 공주대학교 사범대학 과학교육연구소 1996 과학교육연구 Vol.27 No.1

곰팡이 Trichoderma viride 셀룰라아제로부터 세가지의 endoglucanase (endoglucanase Ⅰ,Ⅱ및Ⅲ)가 다양한 크로마토그래피 과정을 통해 분리되었다. 또한 활성에 미치는 tryptophan기의 역할을 화학적 변형을 통하여 알아보았다. N-bromosuccinimide에 의한 효소의 화학적 변형으로부터, endoglucanase Ⅰ,Ⅱ및Ⅲ의 분자당 지니고 있는 tryptophan의 개수가 각각 6,1,1.5개 임을 알수 있었다. 즉 endoglucanase Ⅰ은 분자당 여섯 개의 tryptophan을 함유하고 있으며, 활성부위에는 한 개가 영향을 미치는 것으로 나타났다. 반면에 endoglucanase Ⅱ및Ⅲ는 분자내에 대략 하나의 tryptophan이 단지 활성부위에서만 작용하는 결과를 보여주었다. 또한 endoglucanase Ⅰ의 경우 활성부위가 아닌 다른 부분에 있는 tryptophan기가 기질의 표면에 흡착하는데 미치는 영향을 알아보았다. Three endoglucanases (endoglucanase Ⅰ,Ⅱ and Ⅲ ) as major cellulase components were isolated from the fungus Trichoderma viride by a of chromatography procedures, and the role of tryptophan residues in the activity towards carboxymethyl cellulose were investigated through kinetic and chemical modification studies. Through the modification of cellulase with N-bromosuccin-imide, it was found that endoglucanase Ⅰ,Ⅱ and Ⅲ contained 6, 1, and 1.5 tryptophan residue(s) per enzyme molecule, respectively. From the result of the kinetic data on the modification, it was estimated that the active site of enzyme includes at least one or two tryptophan residue. These results indicated that, in the case of endoglucanase Ⅰ, five tryptophan residues are exposed to another region of the enzyme except that of active site. In the case of the endoglucanase Ⅱ and Ⅲ, however, tryptophan residue exists almost in the active site. In addition, the relationship between endoglucanase activity towards cellulose and the role of tryptophan residue exposed to the enzyme surface was discussed.

父母의 學習能力 認知와 期待水準이 特殊兒의 學業成績에 미치는 影響

류상덕,원영조,권기덕,박태룡 한국사회사업대학 학생지도연구소 1975 학생지도연구 Vol.2 No.-

딕카이트로부터 스멕타이트의 수열합성

류경원,장영남,배인국,채수천,최상훈 한국광물학회 2004 광물과 암석 (J.Miner.Soc.Korea) Vol.17 No.3

딕카이트[Al₂Si₂O_(5).(OH)₄]를 출발물질로 사용하여 이팔면체형 스멕타이트를 수열합성하였다. 시료를 활성화시키기 위해 Na₂O 성분을 첨가하고 800℃에서 4시간 열처리하였다. 합성실험을 Na-0.7 바이델라이트의 화학반응식에 의한 화학양론적 조성에 따라 SiO₂성분을 첨가하였으며 300℃, 70 ㎏f/㎠ 이하의 조건에서 온도, 압력, 시간 등을 변화시키면서 실험을 수행하였다. 합성실험을 위해 약 1리터 용량의 밀폐형 강철재 압력용기를 사용하였다. 스멕타이트를 합성할 수 있는 최적 조건은 반응온도 290℃, 반응시간 48시간, pH 10 및 60 ㎏f/㎠ 의 압력조건인 것으로 확인되었다. 온도, 압력조건 외에 원료물질의 활성화, 반응시간, 반응용액의 초기 pH 등은 결정도에 영향을 미치는 주요 요인으로 작용하였다. 합성결과물에 대한 X-선 회절분석, 에틸렌글리콜 처리, 'Greene-Kelly' 측정법 등의 실험결과, 합성된 스멕타이트는 Na-바이델라이트임이 확인되었다. A hydrothermal process was used to synthesize dioctahedral smectite from dickite [Al₂Si₂O_(5).(OH)₄]. Dickite was previously activated by heating at 800℃ for 4 hours with Na₂CO₃. After the heat-treatment, Sift was added for stoichiometrv. The autoclaving was carried out in closed stainless steel vessel (about 1 liter) at the condition of various temperature, pressure, time etc. High quality smectite could be obtaind by heating at 290℃ under the pressure of 60 ㎏f/㎠ for 48 hours. This experiment reveals that pH of the solution was an important factor and should be maintained at 10 to 11 for the formation of dioctahedral smectite. Be synthesized smectite was identified as Na-beidellite by the treatment of ethylene glycol and Greene-Kelly test.

赤潮原因種 및 毒性種에 관한 文獻的 調査 : Dinophyta(와편모조)를 중심으로

柳奎烈,文成基,李宗南,李元錫 慶星大學校 1990 論文集 Vol.11 No.3

韓國産 잎벌亞目(벌目)에 대한 系統分類學的 硏究 Ⅳ. 즐잎벌亞科에 대한 走査電子顯微鏡的 硏究

柳聖萬,崔元煐,李鍾郁 嶺南大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.13 No.-

As a result of this study the Korean Selandriinae consist of 16 species, 7 genera including 4 newly recorded species, Strongylogaster macula, Aneugmenus temporalis, A japonicus and A. coronatus, from Korea.

삼천지의 환경과 생물다양성 II. 곤충상

류성만,최원영,이미경,이종욱 嶺南大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.12 No.-

저자들은 1992년 9월20일부터 1992년 11월 7일까지 경상북도 경산시 영남대학교 교내에 위치한 삼천지에 대한 곤충상을 조사하였다. 연구 결과 총 8目 56科 121屬 132種이 서식하고 있는 것으로 밝혀졌다. We studied insect fauna in the Samchun-impoundment during the period of from september 20, 1992 to November 7, 1992. The total number of insect taxa are composed of 132 spceies, 121 genera, 56 families, 8 orders including this examination.

퇴행성 슬관절염 환자 평가 설문도구의 내적 신뢰도, 상관성에 관한 연구

류성룡,안경애,서병관,강중원,이상훈,이재동,최도영,김건식,이두익,이윤호 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objective : The aim of this study is to evaluate internal reliability and correlation among assessment instruments of knee OA. Methods : Data were obtained from 85 patients with knee OA. They were asked to answer VAS pain scale, two disease-specific questionnaires (WOMAC and LFI) and one instrument about health-related quality(KHAQ). Reliability was assessed using cronbach's alpha. Correlation was assessed by examining the pearson's correlation coefficients. Results : Cronbach's alpha of all Index except WOMAC stiffness subscale(.6679) exceeded the value of 0.70. A good correlation was obtained between WOMAC Subscale (especially Pain Subscale) and LFI (.726<cronbach's alpha<.791). Also, Correlation between KHAQ and two disease-specific questionnaires (WOMAC and LFI) was good(.612<cronbach's alpha<.662). Conclusions : All used instruments have good internal reliability. also, There are good correlation among them. The treatment of knee OA must be focused on health related quality. For this, we should use proper assessment instruments.

소방구급대원의 응급처치 적절성 평가와 재교육 필요성

류석용,김원율,김경환,이상래,이경호,김홍용,이승한 대한응급의학회 2000 대한응급의학회지 Vol.11 No.2

Background: The prehospital emergency medical system(EMS) for 119 rescue has progressed considerably, but leaves much to be desired. To improve prehospital EMS, we need to evaluate prehospital patient care, and reeducation. Methods: The records of 1,882 patients, who visited Sanggye Paik Hospital Emergency Department via 119 rescue from April 1 to July 30, 1999, were analyzed according to the quality of patient care as documented by the 119 rescue protocol and database. Patients are grouped into 5 classes based on the quality of the care received. Class 1A received adequate care, class 1B received inadequate care, class 1C did not receive the necessary care, and class 2A received unnecessary care, class 2B did not receive unnecessary care. The results of such analyses are presented to the fireman at monthly meetings, thus reeducating them. Results: The overall results were 1027 cases in Class 1A(55%), 83 in 1B(4%), 149 in 1C(8%), 21 in 2A(1%), and 602 in 2B(32%). Well performed care(1A+2B) occured in 1629 cases(87%) and badly performed care(1B+1C+2A) occured in 253cases(13%). Well perfomed care gradually increased from April to July (April 83%, May 85%, June 87%, and July 89%). Conclusion: Meticulous appraisal of the quality of prehospital patient care and reeducation through the regular meetings of EMS physicians and firemen in each community is needed for developing a model protocol for indirect retrospective medical control of the prehospital EMS.

Emulsion형 化粧品의 Escherichia coli 汚染防止에 대한 防腐制效果

柳美淑,金樟圭,元省皓,金男紀 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2

Most of cosmetics are emulsion products that contain the source of nutrition-vegetable oil, mineral oil and carbohydrate etc. -so there is much possibility of contamination by microbials. The purpose of this study is to get the data necessary not only to prevent dermatopathia occurred by Escherichia coli but to sustain the quality and the wear of cosmetics as the result of finding optimum growth condition and the growth condition of Escherichia coli for enviro-factor, utilizing antiseptic so as to prevent contamination by observing it in emulsion type cosmetic. The results are as follows : 1. The optimum growth condition of Escherichia coli : pH7.0 and 37.5℃ The mixing volume ratio of water / oil was 70/30 in this experiment. 2. Adding antiseptics into emulsion type cosmetic, the number of Escherichia coli was decreased from 10^8(cell/ml) to 8 x 10^3(cell/ml) 3. Antibacterial effect against Escherichia coli was better as follows, p-hydroxy benzoic acid propyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid buthyl ester + acetic acid buffer solution > p-hydroxy benzoic methyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid methyl ester + p-hydroxy benzoic acid buthyl ester >p-hydroxy

제 7차 교육과정에 따른 국어과 읽기·쓰기 수행평가 연구(Ⅱ)

류성기,곽재용,천경록,이원희 한국초등국어교육학회 2002 한국초등국어교육 Vol.21 No.-

이 연구는 제7차 교육과정에 따른 초등학교 국어과 읽기와 쓰기 수행평가에 대한 것이다. 쓰기는 교과서의 '되돌아보기' 학습 내용의 타당성을 대부분 인정하고, 대신 '되돌아보기' 학습을 하고 난 후의 정확한 평가 기준표를 제시하고 이를 검증해 보는 것에 역점을 두었다. 특히 거시적 관점에서 10단계의 평가 기준표를 제시하여 학생들의 쓰기 수준이 어느 정도까지 이르렀는가를 측정해 볼 수 있게 하였다. 이 연구는 현장 적용이 가능한 국어와 읽기와 쓰기의 수행평가의 방법을 제시해 주었다는 데 큰 의미가 있다. This study is about the performance assessment for reading and writing of Korean Subject in elementary school being on the 7th Korean National Curriculum. The part of writing puts great emphasis on showing an accurate assesment rubric and verifying it after "review pages", instead of accepting mostly the adequacy of learning contents on "review pages", in textbook. Above other things it suggests an assessment rubric of 10 level and can help to measure what level the writing level of students is in. The great meaning of this study is to suggest the method of the performance assessment for the reading and writing of Korean Subject possible to be applied to the schools. It needs to suggest the method of the intergrative performance assessment for speaking, listening, reading, writing, Korean knowledge, and literature as well. But we will study the above-mentioned remark in future.