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Muhammad Waqas,Muhammad Tehseen Azhar,Iqrar Ahmad Rana,Farrukh Azeem,Muhammad Amjad Ali,Muhammad Amjad Nawaz,정규화,Rana Muhammad Atif 한국유전학회 2019 Genes & Genomics Vol.41 No.4
Background WRKY proteins play a vital role in the regulation of several imperative plant metabolic processes and pathways, especially under biotic and abiotic stresses. Although WRKY genes have been characterized in various major crop plants, their identification and characterization in pulse legumes is still in its infancy. Chickpea (Cicer arietinum L.) is the most important pulse legume grown in arid and semi-arid tropics. Objective In silico identification and characterization of WRKY transcription factor-encoding genes in chickpea genome. Methods For this purpose, a systematic genome-wide analysis was carried out to identify the non-redundant WRKY transcription factors in the chickpea genome. Results We have computationally identified 70 WRKY-encoding non-redundant genes which were randomly distributed on all the chickpea chromosomes except chromosome 8. The evolutionary phylogenetic analysis classified the WRKY proteins into three major groups (I, II and III) and seven sub-groups (IN, IC, IIa, IIb, IIc, IId and IIe). The gene structure analysis revealed the presence of 2–7 introns among the family members. Along with the presence of absolutely conserved signatory WRKY domain, 19 different domains were also found to be conserved in a group-specific manner. Insights of gene duplication analysis revealed the predominant role of segmental duplications for the expansion of WRKY genes in chickpea. Purifying selection seems to be operated during the evolution and expansion of paralogous WRKY genes. The transcriptome data-based in silico expression analysis revealed the differential expression of CarWRKY genes in root and shoot tissues under salt, drought, and cold stress conditions. Moreover, some of these genes showed identical expression pattern under these stresses, revealing the possibility of involvement of these genes in conserved abiotic stress–response pathways. Conclusion This genome-wide computational analysis will serve as a base to accelerate the functional characterization of WRKY TFs especially under biotic and abiotic stresses.
Mariyam,Muhammad Shafiq,Muhammad Haseeb,Rana Muhammad Atif,Syed Agha Armaghan Asad Abbas Naqvi,Numan Ali,Muhammad Arshad Javed,Fizza Gillani,Muhammad Saleem Haider 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.6
DNA binding with one fi nger (Dof) proteins are encoded by a ubiquitous plant-specifi c transcription factor gene family thatplays a critical role in various biological processes including fruit ripening and organogenesis. The wild olive ( Olea europaeavar. sylvestris v1.0 ) genome was used to identify Dof gene family members using a set of bioinformatics tools. Gene structure,chromosome locations, phylogeny, protein motifs, miRNA targets and tissue-specifi c expression patterns were analyzed. Here, we identifi ed 51 potential Dof genes unevenly distributed on all chromosomes and a few scaff olds. Dof proteins in oliveclustered into eight subgroups (D1, B2, C3, C2.2, C1, C2.1, B1, and A) based on the established Arabidopsis classifi cation. The prevalence of segmental duplication was observed as compared to tandem duplication, and this was the main factorunderlying the expansion of the Dof gene family in olive. Tissue-specifi c expression profi ling of Oeu Dof genes revealed thatthe majority of Oeu Dof genes were highly expressed in fl owers, stem and meristem tissues. In seed and meristem tissues,cis-regulatory element (CRE) analysis revealed the presence of elements that are specifi cally responsive to light, circadian,auxin, and ABA. In addition, a comparative analysis between Dof genes in olive and Arabidopsis revealed eight groups orsub-families, although the C3 group of Arabidopsis was not represented in olive. This extensive genome evaluation of theDof gene family in olive presents a reference for cloning and functional analysis of the members of this gene family.
Genetic and Molecular Control of Floral Organ Identity in Cereals
Ali, Zulfiqar,Raza, Qasim,Atif, Rana Muhammad,Aslam, Usman,Ajmal, Muhammad,Chung, Gyuhwa MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.11
<P>Grasses represent a major family of monocots comprising mostly cereals. When compared to their eudicot counterparts, cereals show a remarkable morphological diversity. Understanding the molecular basis of floral organ identity and inflorescence development is crucial to gain insight into the grain development for yield improvement purposes in cereals, however, the exact genetic mechanism of floral organogenesis remains elusive due to their complex inflorescence architecture. Extensive molecular analyses of Arabidopsis and other plant genera and species have established the ABCDE floral organ identity model. According to this model, hierarchical combinatorial activities of A, B, C, D, and E classes of homeotic genes regulate the identity of different floral organs with partial conservation and partial diversification between eudicots and cereals. Here, we review the developmental role of A, B, C, D, and E gene classes and explore the recent advances in understanding the floral development and subsequent organ specification in major cereals with reference to model plants. Furthermore, we discuss the evolutionary relationships among known floral organ identity genes. This comparative overview of floral developmental genes and associated regulatory factors, within and between species, will provide a thorough understanding of underlying complex genetic and molecular control of flower development and floral organ identity, which can be helpful to devise innovative strategies for grain yield improvement in cereals.</P>