Posttranscriptional deregulation of Src due to aberrant miR34a and miR203 contributes to gastric cancer development

Qiang Hao,Xiao Zhao Lu,Nan Nan Liu,Xiao Chang Xue,Meng Li,Cun Zhang,Xin Qin,Wei Na Li,Zhen Shu,Bin Song,Qing Wang,Li Qiang Song,Wei Zhang,Ying Qi Zhang 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.6

A magnetostrictive self-powered biosensor based on Au-BaTiO3-FeGa & PDMS

Qiang Zhang,Meng Xu,Yan Liu,Chunyan Zhang,Rui Zhang,Zhiyuan Fu,Jianlong Ji,Riguang Zhang,Shengbo Sang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

Piezoelectric flexible sensors have been used to detect biomolecules such as sweat and glucose because oftheir passive, simple structure and high sensitivity. This paper proposes a novel flexible piezoelectric Au-BaTiO3-FeGa & PDMS biosensor in which magnetostrictive deformation amplifies the surface stress generatedby biomolecules combining on the thin film. The modification process of bovine serum albumin(BSA) binding with the sensor was initially determined by the first principles approach. Then, the sensingmechanism was verified by finite-element simulation. Based on the simulation results, flexible Au-BaTiO3-FeGa & PDMS biosensors were prepared, modified, and measured. The structure, modification,and detection of the sensors were analyzed by digital microscopy, Fourier transform infrared spectrometry(FTIR), atomic force microscopy (AFM), and scanning electron microscopy (SEM). The responses ofthe biosensors detecting different BSA solution concentrations under magnetic fields were then investigated. Experimental results indicate that the biosensor has the highest sensitivity under a magnetic fieldof 30 mT.

Potential Therapeutic Targets for the Primary Gallbladder Carcinoma: Estrogen Receptors

Zhang, Ling-Qiang,Zhang, Xiu-De,Xu, Jia,Wan, Yong,Qu, Kai,Zhang, Jing-Yao,Wang, Zhi-Xin,Wei, Ji-Chao,Meng, Fan-Di,Tai, Ming-Hui,Zhou, Lei,Liu, Chang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Gallbladder carcinoma, the most frequent malignant neoplasm of the biliary tract system, has always been considered to feature late clinical presentation and diagnosis, limited treatment options and an extremely poor prognosis. In recent years, while the incidence of gallbladder cancer has appeared to be on the increase, the available treatment methods have not greatly improved survival of the affected patients. Thus, exploring new therapeutic targets for this devastating disease is an urgent matter at present. Epidemical studies have demonstrated that the incidence of gallbladder carcinoma exhibits a distinct gender bias, affecting females two to three times more than males, pointing to crucial roles of estrogen. It is well known that estrogen acts on target tissues by binding to estrogen receptors (ERs), which are mainly divided into three subtypes, $ER{\alpha}$, $ER{\beta}$ and $ER{\gamma}$. $ER{\alpha}$ and $ER{\beta}$ appear to have overlapping but also unique even opposite biological effects. As important pathogenic mediators, ERs have been considered to relate to several kinds of tumors. In gallbladder carcinoma tissue, ERs have been shown to be positively expressed, and ERs expression levels are associated with differentiation and prognosis of this cancer. Nevertheless, the exact mechanisms of estrogen inducing growth of gallbladder carcinoma remain poorly understood. On the base of the current investigations, we deduce that estrogen participates in promotion of gallbladder carcinoma by influencing the formation of gallstones, stimulating angiogenesis, and promoting abnormal proliferation. Since ERs mediate the carcinogenic actions of estrogen in gallbladder, and therapy targeting ERs may provide new directions for gallbladder carcinoma. Therefore, it should be stressed that ERs are potential therapeutic targets for gallbladder carcinoma.

A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection

Junbo Zhang,Shuanghong Yin,Fei Guo,Ren Meng,Chuangfu Chen,Hui Zhang,Zhi-qiang Li,Qiang Fu,Huijun Shi,Shengwei Hu,Wei Ni,Tiansen Li,Ke Zhang 한국미생물학회 2014 The journal of microbiology Vol.52 No.8

Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucellaspecific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.

Model Reference Robust Adaptive H∞ Controller Design

Qiang Zhang,De-Zhi Xu,Kang-Kang Zhang 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.4

In this paper, a novel model reference robust adaptive H∞ controller is designed, which not only guaranteesasymptotically stability, but also optimizes adaptive H∞ performance by estimating the optimal unknowncontrol parameters. Furthermore, a novel state feedback framework is introduced, which depends on the optimalunknown parameter estimations, to guarantee that the defined cost function is minimized. At the same time, theminimal adaptive H∞ performance index is obtained.

Sonication-Assisted Synthesis of Molybdenum Disulfide Aerogel for the Electrode Materials of Supercapacitors

Qiang Zhang,Wenyuan He,Yinmin Wang,Dazhao Pei,Xue Jun Zheng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.5

The sonication processing was added in front of the freeze-drying as an intermediate processing before the molybdenum disulfide (MoS2) aerogel was synthesized. It is distinguishing with the traditional hydrothermal reaction to combine the sonication processing and freeze-drying in our method. The structure, morphology, specific surface area and pore size distribution were characterized, and the electrochemical performances were measured in 0.5M Na2SO4 electrolyte for the MoS2 aerogel and flower-like MoS2. As for comparison, they are of porous structure and microsphere structure, and their specific surface areas are 55.14 m2 g -1 and 38.12 m2 g -1. The specific capacitances are 166.7 F g -1 and 119.2 F g -1 at the scan rate of 5 mV s -1, and the capacity retentions are 87.7% and 81.6% after 3000 charge/discharge cycles. For the enhanced mechanism, the high specific surface of the MoS2 aerogel causes high specific capacitance, and the unique porous structure could buffer volume expansion to improve retention ability during charge/discharge processes. The MoS2 aerogel may thus be a promising electrode material for supercapacitors.

Operation start time and long-term outcome of hepatocellular carcinoma after curative hepatic resection

Qiang Lu,Qing-Shan Li,Wei Zhang,Kang Liu,Tao Li,Jia-Wei Yu,Yi Lv,Xu-Feng Zhang 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.1

Purpose: The objective of the current study was to examine the potential effects of surgery start times (morning vs. afternoon) on the long-term prognosis of patients after hepatic resection (HR) for hepatocellular carcinoma (HCC). Methods: All eligible patients were divided into 2 groups according to the start time of surgery: group M (morning surgery, 8 AM–1 PM) and group A (afternoon surgery, 1 PM–6 PM). Clinicopathologic and surgical parameters, as well as oncologic outcomes were compared between the 2 groups. Results: In total, 231 patients were included in the study. There was no difference in age, body mass index, comorbidities, tumor size, tumor location, tumor stages, surgical procedures, or surgical margin between morning and afternoon surgery (all P > 0.05). In contrast, patients in group M experienced longer operation duration than those in group A (median, 240 minutes vs. 195 minutes, P = 0.004). However, no differences of overall survival were observed between morning and afternoon surgery groups in the whole cohort or stratified by surgical margin (all P > 0.05). Conclusion: Surgery start times during the work day have no measurable influence on patient outcome following curative HR for HCC, indicating good self-regulation and professional judgment of surgeons for progressive fatigue during surgery

Facile Preparation of Mechanical Reinforced and Biocompatible Silk Gels

Qiang Zhang,Guocong Han,Chen Lu,Qiusheng Wang,Xiufang Li,Zuwei Luo,Renchuan You,Shuqin Yan 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4

Nontoxic and controllable way to fabricate silk fibroin (SF) gel with high mechanical properties is of critical important to biomaterial in tissue engineering. Electrochemically triggered electrophoretic migration and electric charge of the silk molecules both contributed to SF microspheres and electronic gel (e-gel) formation by sol-gel transition. In this study, a novel silk pH e-gel with higher mechanical property was prepared by combining low-voltage electric fields with isoelectric point (pI) adjustment. This green process was mild and friendly without chemical crosslinker. Compressive modulus of the silk pH e-gel was up to 70 MPa that was significantly higher than that of SF gelation spontaneously. Furthermore, analysis of molecule conformation of the silk pH-e-gel demonstrated that most of random coil structures transformed into α-helix and a little β-sheet structures during this process. The silk pH e-gel was loaded with rhodamine B and showed an obvious sustainable release profile. Accumulation releasing amounts was approximately 60 % at day 9. Cytocompatibility of the silk pH-e-gel was evaluated by epithelial cell. The results showed that the gels could support the cell growth and proliferation in vitro. Finally, gel biodegradation was assessed by protease XIV. After biodegradation for 28 days, remaining weight of the gel was about 20.23±2.59 wt%, indicating its good biodegradability. This novel process was established successfully by combining low voltage field with pH-control, which provided an alternative material for regenerative medicine.

CORROSION BEHAVIOR OF NI-BASE ALLOYS IN SUPERCRITICAL WATER

Zhang, Qiang,Tang, Rui,Li, Cong,Luo, Xin,Long, Chongsheng,Yin, Kaiju Korean Nuclear Society 2009 Nuclear Engineering and Technology Vol.41 No.2

Corrosion of nickel-base alloys (Hastelloy C-276, Inconel 625, and Inconel X-750) in $500^{\circ}C$, 25 MPa supercritical water (with 10 wppb oxygen) was investigated to evaluate the suitability of these alloys for use in supercritical water reactors. Oxide scales formed on the samples were characterized by gravimetry, scanning electron microscopy/energy dispersive spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The results indicate that, during the 1000 h exposure, a dense spinel oxide layer, mainly consisting of a fine Cr-rich inner layer ($NiCr_2O_4$) underneath a coarse Fe-rich outer layer ($NiFe_2O_4$), developed on each alloy. Besides general corrosion, nodular corrosion occurred on alloy 625 possibly resulting from local attack of ${\gamma}"$ clusters in the matrix. The mass gains for all alloys were small, while alloy X-750 exhibited the highest oxidation rate, probably due to the absence of Mo.

Clinical Significance of SH2B1 Adaptor Protein Expression in Non-small Cell Lung Cancer

Zhang, Hang,Duan, Chao-Jun,Chen, Wei,Wang, Shao-Qiang,Zhang, Sheng-Kang,Dong, Shuo,Cheng, Yuan-Da,Zhang, Chun-Fang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

The SH2B1 adaptor protein is recruited to multiple ligand-activated receptor tyrosine kinases that play important role in the physiologic and pathologic features of many cancers. The purpose of this study was to assess SH2B1 expression and to explore its contribution to the non-small cell lung cancer (NSCLC). Methods: SH2B1 expression in 114 primary NSCLC tissue specimens was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients' outcome. Additionally, 15 paired NSCLC background tissues, 5 NSCLC cell lines and a normal HBE cell line were evaluated for SH2B1 expression by RT-PCR and immunoblotting, immunofluorescence being applied for the cell lines. Results: SH2B1 was found to be overexpressed in NSCLC tissues and NSCLC cell lines. More importantly, high SH2B1 expression was significantly associated with tumor grade, tumor size, clinical stage, lymph node metastasis, and recurrence respectively. Survival analysis demonstrated that patients with high SH2B1 expression had both poorer disease-free survival and overall survival than other patients. Multivariate Cox regression analysis revealed that SH2B1 overexpression was an independent prognostic factor for patients with NSCLC. Conclusions: Our findings suggest that the SH2B1 protein may contribute to the malignant progression of NSCLC and could offer a novel prognostic indicator for patients with NSCLC.