Root Health Evaluation of Different Alfalfa Varieties in Agricultural and Animal Husbandly Areas

Qi-zhong Sun,Li-jun Xu,Zhu Yu,Shu-fen Zhao 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Research on alfalfa root morphology and health is very important in development of technology for efficient improvement and production of alfalfa. The objectives of this study were to evaluate the root morphology and health of two alfalfa varieties, Algonquin and Golden Queen, to determine relationships among root morphology traits and root health. Results from these analyses indicated t㏊t yield was 6.77-43.93 t/h㎡, total root length ranged from 215.17-708.89 ㎜, root surface area ranged from 93.61-717.06 ㎠, volume ranged from 3.24-57.72㎤. Results from these analyses indicated t㏊t root infestation score was the lowest averaged over age of alfalfa stand in Algonquin. Yield in 2-yr old stands was greater in Golden Queen compared to Algonquin.

A Margin Morphology-Based Gross Classification System for Stratifying Hepatocellular Carcinoma

( Zhong-qi Fan ),( Xiao-dong Sun ),( Ming-yue Li ),( Mei-shan Jin ),( Lei Zhang ),( Nan-ya Wang ),( Tian Yang ),( Guo-yue Lv ) 대한간학회 2024 춘·추계 학술대회 (The Liver Week) Vol.2024 No.1

Charge separation behaviors of novel AgI/BiOI heterostructures with enhanced solar-photocatalytic performance

Qi Yang,Jiao Huang,Junbo Zhong,Jiufu Chen,Jianzhang Li,Siyang Sun 한국물리학회 2017 Current Applied Physics Vol.17 No.9

The photocatalytic efficiency of the bare BiOI has been greatly restricted because of the high recombination rate of electron-hole charge pairs. To further regulate the photocatalytic performance of BiOI, in this work AgI was employed to couple with BiOI. A serial of novel AgI/BiOI heterostructures with improved sunlight-driven photocatalytic activities were prepared in-situ by a hydrothermal method. The results reveal that the presence of AgI in the composites alters band gap of the samples and greatly affects the photo-induced charge separation rate benefited from the strong interaction between AgI and BiOI. $OH and $O2 were observed using electron spin-resonance (ESR) spectroscopy. The photocatalytic activities of AgI/BiOI heterostructures for the decay of methyl orange (MO) and phenol solution under the simulated sunlight irradiation were investigated, the results display that the composite photocatalysts exhibit higher photocatalytic activity than the bare BiOI and excellent stability. The charge separation and transfer mechanism was proposed based on the experimental results.

Enzymatic Synthesis of Theanine with L-glutamine-Zn(II) Complexes

Hao-Qi Wang,Zhong Yao,Zhi Zhou,Yun Sun,Ping Wei,Ping-Kai Ouyang 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6

Theanine, a unique amino acid found in tea plants, has many important physiological functions. Theanine can be enzymatically synthesized via the γ-glutamyltranspeptidation reaction. In this study, we described a new method of theanine synthesis using the L-glutamine-Zn(II)(Zn(Gln)2) complex instead of glutamine as the donor,which successfully reduced the side autotranspeptidation reaction and led to higher yield of theanine. We prepared the Zn(Gln)2 complexes and showed that they are stable in liquid bulk under 9.0 pH. After using the Zn(Gln)2 in the γ-glutamyltranspeptidation reaction, we utilized HPLC and Mass spectrometry analysis to demonstrated that Zn(Gln)2was an more effective γ-glutamyl donor than glutamine. The autotranspeptidation reaction was restrained effectively. As a result, the theanine yield and the conversion rate for glutamine were vastly improved. In a reaction mixture containing 48 mM of Zn(Gln)2, 1.6 M ethylamine, and 0.5 U/mL GGT, the final concentration of theanine obtained was 61.3 mM after incubation at 37oC for three hours. The conversion rate for glutamine was 63.8%, which showed a 16.9% increase as compared to when using glutamine alone as the donor substrate.

Endovascular Repair of Blunt Popliteal Arterial Injuries

Shan Zhong,Xiquan Zhang,Zhong Chen,Peng Dong,Yequan Sun,Wei Zhu,Xiaolin Pan,Deming Qi 대한영상의학회 2016 Korean Journal of Radiology Vol.17 No.5

Objective: To evaluate the feasibility and effectiveness of endovascular repair for blunt popliteal arterial injuries. Materials and Methods: A retrospective analysis of seven patients with clinical suspicion of popliteal arterial injuries that were confirmed by arteriography was performed from September 2009 to July 2014. Clinical data included demographics, mechanism of injury, type of injury, location of injury, concomitant injuries, time of endovascular procedures, time interval from trauma to blood flow restoration, instrument utilized, and follow-up. All patients were male (mean age of 35.9 ± 10.3 years). The type of lesion involved intimal injury (n = 1), partial transection (n = 2), complete transection (n = 2), arteriovenous fistula (n = 1), and pseudoaneurysm (n = 1). All patients underwent endovascular repair of blunt popliteal arterial injuries. Results: Technical success rate was 100%. Intimal injury was treated with a bare-metal stent. Pseudoaneurysm and popliteal artery transections were treated with bare-metal stents. Arteriovenous fistula was treated with bare-metal stent and coils. No perioperative death and procedure-related complication occurred. The average follow-up was 20.9 ± 2.3 months (range 18–24 months). One patient underwent intra-arterial thrombolysis due to stent thrombosis at 18 months after the procedure. All limbs were salvaged. Stent migration, deformation, or fracture was not found during the follow-up. Conclusion: Endovascular repair seems to be a viable approach for patients with blunt popliteal arterial injuries, especially on an emergency basis. Endovascular repair may be effective in the short-term. Further studies are required to evaluate the long-term efficacy of endovascular repair.

Selection signature reveals genes associated with susceptibility loci affecting respiratory disease due to pleiotropic and hitchhiking effect in Chinese indigenous pigs

Xu, Zhong,Sun, Hao,Zhang, Zhe,Zhang, Cheng-Yue,Zhao, Qing-bo,Xiao, Qian,Olasege, Babatunde Shittu,Ma, Pei-Pei,Zhang, Xiang-Zhe,Wang, Qi-Shan,Pan, Yu-Chun Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.2

Objective: Porcine respiratory disease is one of the most important health problems causing significant economic losses. To understand the genetic basis for susceptibility to swine enzootic pneumonia (EP) in pigs, we detected 102,809 single nucleotide polymorphisms in a total of 249 individuals based on genome-wide sequencing data. Methods: Genome comparison of susceptibility to swine EP in three pig breeds (Jinhua, Erhualian, and Meishan) with two western lines that are considered more resistant (Duroc and Landrace) using cross-population extended haplotype homozygosity and F-statistic (F_ST) statistical approaches identified 691 positively selected genes. Based on quantitative trait loci, gene ontology terms and literature search, we selected 14 candidate genes that have convincible biological functions associated with swine EP or human asthma. Results: Most of these genes were tested by several methods including transcription analysis and candidate genes association study. Among these genes: cytochrome P450 1A1 and catenin beta 1 (CTNNB1) are involved in fertility; transforming growth factor beta receptor 3 plays a role in meat quality traits; Wnt family member 2, CTNNB1 and transcription factor 7 take part in adipogenesis and fat deposition simultaneously; plasminogen activator, urokinase receptor (completely linked to AXL receptor tyrosine kinase, r² = 1) plays an essential role in the successful ovulation of matured oocytes in pigs; colipase like 2 (strongly linked to SAM pointed domain containing ETS transcription factor, r² = 0.848) is involved in male fertility. Conclusion: These adverse genes susceptible to swine EP may be selected while selecting for economic traits (especially reproduction traits) due to pleiotropic and hitchhiking effect of linked genes. Our study provided a completely new point of view to understand the genetic basis for susceptibility or resistance to swine EP in pigs thereby, provides insight for designing sustainable breed selection programs. Finally, the candidate genes are crucial due to their potential roles in respiratory diseases in a large number of species, including human.

Aspirin inhibits lipopolysaccharide-induced COX-2 expression and PGE2 production in porcine alveolar macrophages by modulating protein kinase C and protein tyrosine phosphatase activity

( Yu Zhong Duan ),( Fang Lin Chen ),( An Mei Zhang ),( Bo Zhu ),( Jian Guo Sun ),( Qi Chao Xie ),( Zheng Tang Chen ),( Zheng Tang Chen ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.1

Aspirin has been demonstrated to be effective in inhibiting COX-2 and PGE2 in Alveolar macrophages (AMs). However, the mechanisms have not been fully understood. In the present study, we found that pretreatment with aspirin inhibited LPS-induced COX-2 and PGE2 upregulation, IkBa degradation, NFkB activation and the increase of PKC activity, but elevated LPS-induced the decrease of PTP activity. The PKC inhibitor calphostin C dramatically reduced the COX-2 mRNA and PGE2 levels, but the PTP inhibitor peroxovanadium (POV) significantly increased the COX-2 mRNA and PGE2 levels. Furthermore, the PTP inhibitor mitigated the inhibitory effect of aspirin on COX-2 and PGE2 upregulation and NF-kB activation, whereas the PKC inhibitor enhanced the inhibitory effects of aspirin on the production of COX-2 and PGE2. Our data indicate a novel mechanism by which aspirin acts as a potent anti-inflammatory agent in alveolus macrophages and ALI. [BMB Reports 2014; 47(1): 45-50]

Dynamic Variation of Some Matter Related to Cold Resistance in Medicago sativa under Chilling Stress

Ya Tao,Qi-zhong Sun,Zhu Yu,Shu-fen Zhao 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

In this study, we determinated the dynamic changing of soluble sugar, total nitrogen and malondialdehyde, so t㏊t found out their relationships with cold resistance. The result indicates: the content of soluble sugar and total nitrogen increased from late autumn and early winter, and kept higher level during the entire winter, then decreased in regreen time in the next spring. The content of malondialdehyde was high in Sep., then decreased gradually and reached the minimum in Jan., thereafter the content of malondialdehyde represented increasing trend.

Clinical Application of Recombinant Human Endostatin in Postoperative Early Complementary Therapy on Patients with Non-small Cell Lung Cancer in Chinese Mainland

Zhu, Qiang,Zang, Qi,Jiang, Zhong-Min,Wang, Wei,Cao, Ming,Su, Gong-Zhang,Zhen, Tian-Chang,Zhang, Xiao-Tian,Sun, Ning-Bo,Zhao, Cheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Objective: To explore the clinical application of recombinant human endostatin (Endostar) in the treatment of patients with non-small cell lung cancer (NSCLC) in Chinese mainland. Materials and Methods: A total of 75 patients diagnosed as NSCLC were randomly divided into control group (37 cases) and treatment group (38 cases). Control group was treated with postoperative complementary chemotherapy containing two-agent platinum protocol on postoperative d21, 3 weeks as a cycle, for totally 4~6 cycles. On this basis, treatment group was added with Endostar $7.5mg/m^2$ on postoperative d8~9, 3~4 h/time, qd, 14 weeks as a cycle, for totally 4 cycles. The interval between every two cycles was 7 d. The 5-year progression-free survival (PFS), 5-year survival time and complications in both groups were observed. Results: Compared with control group, the average PFS increased evidently in treatment group by 9.8 months (41.6 months vs. 31.8 months), and there was significant difference (P<0.05). And the median PFS was 42.5 months in treatment group, obviously longer than that in control group (33.7 months) by 8.8 months (P<0.05). Additionally, the 5-year overall survival rate (OS), average survival time and median survival time (MST) were 47.4%, 50.1 months and 59.3 months in treatment group, significantly higher than the 29.7%, 42.1 months and 43.5 months in control group (P<0.05). Only 1 patient showed poor healing of surgical wound in treatment group, but no surgery-associated complication was found in control group. Moreover, the postoperative complementary therapy-connected complication rates were 63.2% (24/38) and 59.5% (22/37) in treatment group and control group respectively, but there was no significant difference (P>0.05). Conclusions: The application of Endostar combined with sensitive platinum-contained chemotherapeutic agents in the postoperative complementary chemotherapy can be widely used in clinic because it can significantly prolong the long-term survival time of patients with NSCLC.

Study on the Flavonoids of Lespedeza hedysaroides

Yu-qing Wang,Alamus,Qi-zhong Sun,Hong-xin Wu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Chemical investigation of the aerial parts of Lespedaza hedysaroides resulted in the isolation of 8 flavonoids:orientin (1), isoorientin (2), vitexin (3), isovitexin (4), Isomyricitrin (5), meletin-3-O-β-d-glucoside (6), luteolin-7-O-glucoside (7), 6-Xylopyranosylluteolin (8), The result showed t㏊t Lespedeza hedysaroides was rich in flavonoid glycosides, espically of C-glycosylflavones which have good Pharmacia and bioactive. So Lespedeza hedysaroides was concluded that it has vast prospect for exploitation and utilization.