http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Pandey Bishnu Prasad,Pradhan Suman Prakash,Adhikari Kapil,Shresth Rajib Kumar 한국미생물학회 2021 미생물학회지 Vol.57 No.3
Streptomyces are widely distributed in soil and known for the production of bioactive secondary metabolites. It has been reported that among microbial-derived antibiotics, two-third are produced by the Streptomyces species alone. Hence, continuous screening of the Streptomyces species is of growing scientific interest. A small Himalayan country like Nepal is in a unique geographical location with a huge biodiversity. However, little is known about microbial diversity. The aim of this study was to isolate and characterize the Streptomyces species from a high-altitude soil sample collected from an altitude of 4,380 meters above sea level. The 16S rRNA sequence analysis revealed that four isolated strains; G-10, G-14, G-18, and S4L belong to the Streptomyces species. On the basis of phylogenetic analysis of 16S rRNA gene sequences of the isolates with the best match in the database revealed that G-18 isolates closely related to Streptomyces albidoflavus strain PAS-12. Moreover, the ranges of radical scavenging activities by crude extract of isolates were observed against DPPH and ABTS. Furthermore, crude extract of isolates revealed the range of antimicrobial activities against the four pathogenic strains namely Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus species, and Bacillus substilis. Moreover, isolated Streptomyces species revealed amylase, cellulase, and L-asparaginase enzyme activities.
Pandey Bishnu Prasad,Pradhan Suman Prakash 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.1
The aim of this study was to examine antioxidant properties, major enzyme inhibition activities, and targeted metabolites profiling of Lepisorus mehrae, Pleurospermum benthamii, and Roscoea auriculata in different solvent extracts. This is the first report on metabolites profiling and biochemical activities of these plant species. Our results revealed that L. mehrae, P. benthamii, and R. auriculata are rich source of bioactive secondary metabolites and have good antioxidant potential. The methanol extract of L. mehrae, P. benthamii, and R. auriculata showed substantial inhibitory potential towards elastase, whereas water extract of L. mehrae and R. auriculata were more strong inhibitors of tyrosinase. Among the three plants, P. benthamii showed noteworthy inhibition on α-amylase, α-glucosidase, lipase, tyrosinase, elastase, and cholinesterases enzymes. High resolution mass spectrometry analysis revealed the presence of metabolites such as Protocatechuic acid, Gal- lic acid, 7,8,3′,4′-tetrahydroxyflavanone, Rhamnocitrin, Quercetin, Hyperoside, Quercetin-7-glucoside, Rutin, Rhamnetin, Aromadendrin, Camphor, and Hexanoic acid in L. mehrae. Moreover, Catechin and Fisetin were present in P. benthamii and Kaempferide, 7,8,3′,4′-Tetrahydroxyflavone, 5,7-Dihydroxy-2,3-dihydroflavonol 3-acetate, and Aromadendrin were present in R. auriculata. The results presented here provide enough scientific evidence that these plant species have diverse biochemi- cal potential and can be examined further for their potential use in modern pharmaceuticals, cosmetics, and nutraceuticals.