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Zhang Wentao,Zheng Zongtai,Wang Keyi,Mao Weipu,Li Xue,Wang Guangchun,Zhang Yuanyuan,Huang Jianhua,Zhang Ning,Wu Pengfei,Liu Ji,Zhang Haimin,Che Jianping,Peng Bo,Zheng Junhua,Li Wei,Yao Xudong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Accumulating studies have confirmed that PIWI-interacting RNAs (piRNAs) are considered epigenetic effectors in cancer. We performed piRNA microarray expression analysis on renal cell carcinoma (RCC) tumor tissues and paired normal tissues and performed a series of in vivo and in vitro experiments to explore piRNAs associated with RCC progression and investigate their functional mechanisms. We found that piR-1742 was highly expressed in RCC tumors and that patients with high piR-1742 expression had a poor prognosis. Inhibition of piR-1742 significantly reduced tumor growth in RCC xenograft and organoid models. Mechanistically, piRNA-1742 regulates the stability of USP8 mRNA by binding directly to hnRNPU, which acts as a deubiquitinating enzyme that inhibits the ubiquitination of MUC12 and promotes the development of malignant RCC. Subsequently, nanotherapeutic systems loaded with piRNA-1742 inhibitors were found to effectively inhibit the metastasis and growth of RCC in vivo. Therefore, this study highlights the functional importance of piRNA-related ubiquitination in RCC and demonstrates the development of a related nanotherapeutic system, possibly contributing to the development of therapeutic approaches for RCC.
Terahertz Characteristics of Hydroxygraphene Based on Microfluidic Technology
Boyan Zhang,Siyu Qian,Bo Peng,Bo Su,Zhuang Peng,Hailin Cui,Shengbo Zhang,Cunlin Zhang 한국광학회 2023 Current Optics and Photonics Vol.7 No.4
Hydroxygraphene as a kind of functionalized graphene has important applications in composite, photoelectric and biological materials. In the present study, THz and microfluidic technologies were implemented to study the THz transmission characteristics of hydroxygraphene with different concentrations and residence times in magnetic and electric fields. The results show that the THz transmission intensity decreases with the increase in sample concentration and duration of an applied electric field, while it increases by staying longer in the magnetic field. The phenomenon is analyzed and explained in terms of hydrogen bond, conductivity and scattering characteristics. The results establish a foundation for future research on the THz absorption characteristics of liquid graphene based on microfluidic technology in different external environments. It also provides technical support for the application and development of graphene in THz devices.
Zhang, Dong-Zhi,Zhang, Ke,Wang, Xiao-Peng,Cai, Hui Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1
Background: Surgical resection of spontaneously ruptured hepatocellular carcinoma (HCC) after successful transarterial embolization (TAE) remains controversial. The aim of this study was to investigate its efficacy in a series of cases. Materials and Methods: We retrospectively examined ruptured HCC cases from Jan 2000 to Dec 2008; all of these 126 cases received TAE as the initial therapy. Subsequently, 74 cases received staged surgical resection, and the remaining 52 cases underwent repeated TACE. The baseline demographic data, tumor characteristics, and long term survival were recorded and compared. Results: The demographic and baseline characteristics were comparable between the hepatic resection and TACE groups; furthermore, no significant difference in the tumor characteristics was detected between the two groups. The differences in in-hospital, 30-day and 90-day mortality between the two groups were not significant (P>0.05). However, the 1-, 3-, and 5-year overall survival rates were 85.1%, 63.5%, and 37.8%, respectively, in the hepatic resection group, which were significantly higher than those in the TACE group (69.2%, 46.2%, and 17.3%, respectively, P=0.004). Univariate and multivariate analyses indicated that these patients benefitted from hepatic resection compared with TACE with respect to long-term outcomes. Conclusions: Staged hepatic resection after TAE is an effective treatment that results in superior long-term survival to repeated TACE.
Inhibitory Effects of Syk Transfection on Lung Cancer Cell Invasion
Peng, Chuan-Liang,Zhang, Ying,Sun, Qi-Feng,Zhao, Yun-Peng,Hao, Ying-Tao,Zhao, Xiao-Gang,Cong, Bo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Objective: Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro. Methods: A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined. Results: The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05). Conclusions: A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.
ZHANG, Peng,LEE, Kwang-Hee,LEE, Chul-Hee Elsevier 2014 Transactions of Nonferrous Metals Society of China Vol.24 No.1
Reciprocating friction characteristics of magneto-rheological (MR) fluid for aluminum under a magnetic field at different loads and oscillation frequencies were studied when MR fluids were worked in reciprocating motions such as in dampers for automobiles, and surface polishing and other finishing. Thus, experiments were carried out to evaluate the reciprocating friction characteristic of MR fluid for aluminum. The obtained data from the tests are sorted in groups depending on various loads and oscillation frequencies, to analyze the relationship between test condition and travel cycle. Surfaces of specimens were compared by measuring the surface roughness and observing the surface images. The performance of reciprocating friction characteristics of MR fluid for aluminum is evaluated through analyzing the experiment results.
Peng Zhang,Yanan Gao,Zohre Moradi,Yasar Ameer Ali,Mohamed Amine Khadimallah 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.44 No.3
The present study tackles the problem of forced vibration of imperfect axially functionally graded shell structure with truncated conical geometry. The linear and nonlinear large-deflection of the structure are considered in the mathematical formulation using von-Kármán models. Modified coupled stress method and principle of minimum virtual work are employed in the modeling to obtain the final governing equations. In addition, formulations of classical elasticity theory are also presented. Different functions, including the linear, convex, and exponential cross-section shapes, are considered in the grading material modeling along the thickness direction. The grading properties of the material are a direct result of the porosity change in the thickness direction. Vibration responses of the structure are calculated using the semi-analytical method of a couple of homotopy perturbation methods (HPM) and the generalized differential quadrature method (GDQM). Contradicting effects of small-scale, porosity, and volume fraction parameters on the nonlinear amplitude, frequency ratio, dynamic deflection, resonance frequency, and natural frequency are observed for shell structure under various boundary conditions.
Zhang, Peng,Park, Guenyoung,Kang, Seong Ho Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.4
A multi-channel microchip electrophoresis (MCME) method with parallel laser-induced fluorescence (LIF) detection was developed for rapid screening of H1N1 virus. The hemagglutinin (HA) and nucleocapsid protein (NP) gene of H1N1 virus were amplified using polymerase chain reaction (PCR). The amplified PCR products of the H1N1 virus DNA (HA, 116 bp and NP, 195 bp) were simultaneously detected within 25 s in three parallel channels using an expanded laser beam and a charge-coupled device camera. The parallel separations were demonstrated using a sieving gel matrix of 0.3% poly(ethylene oxide) ($M_r$ = 8,000,000) in $1{\times}$ TBE buffer (pH 8.4) with a programmed step electric field strength (PSEFS). The method was ~20 times faster than conventional slab gel electrophoresis, without any loss of resolving power or reproducibility. The proposed MCME/PSEFS assay technique provides a simple and accurate method for fast high-throughput screening of infectious virus DNA molecules under 400 bp.
Hydraulic fracture simulation of concrete using the SBFEM-FVM model
Peng Zhang,Chengbin Du,Wenhu Zhao,Deheng Zhang 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.80 No.5
In this paper, a hybrid scaled boundary finite element and finite volume method (SBFEM-FVM) is proposed for simulating hydraulic-fracture propagation in brittle concrete materials. As a semi-analytical method, the scaled boundary finite element method is introduced for modelling concrete crack propagation under both an external force and water pressure. The finite volume method is employed to model the water within the crack and consider the relationship between the water pressure and the crack opening distance. The cohesive crack model is used to analyse the non-linear fracture process zone. The numerical results are compared with experimental data, indicating that the F-CMOD curves and water pressure changes under different loading conditions are approximately the same. Different types of water pressure distributions are also studied with the proposed coupled model, and the results show that the internal water pressure distribution has an important influence on crack propagation.