Macrophages Promote Coal Tar Pitch Extract-induced Tumorigenesis of BEAS-2B Cells and Tumor Metastasis in Nude Mice Mediated by AP-1

Zhang, Peng,Jin, Yue-Fei,Zhang, Qiao,Wu, Yi-Ming,Wu, Wei-Dong,Yao, Wu,Wu, Yong-Jun,Li, Zhi-Tao,Zhao, Yong,Liu, Yu,Feng, Fei-Fei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.

Thin Film Fabrications : PREPARATION AND CHARACTERIZATION OF CdTe AND HgCdTe BY HOT WALL MOCVD

Peng Rui Wu,Ding Yong Qing 대한금속재료학회 ( 구 대한금속학회 ) 1995 Advanced Material and Processing Vol.1 No.-

Harris Scale Invariant Corner Detection Algorithm Based on the Significant Region

Wu Peng,Xu Hongling,Li Wenlin,Song Wenlong 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.3

The traditional Harris corner detection algorithm is sensitive to scale change, corners detected throughout the entire image under complex background, thus extracting more false corners, lead to the follow-up of large amount of calculation and a high rate of error matching. To solve this problem, this paper proposes an optimized Harris corner detection algorithm. First, a significant region detection method is used to extract the target area, and take closing operation for the result figure, can effectively achieve target and background segmentation; second, scale invariant describing methods is applied to Harris algorithm, at the same time, combined with the non-maximum suppression methods to extract corners, get more right corners. Through experiment contrasts, the algorithm used in this paper can be improved more corner detection performance.

An Improved Two-Dimensional Run-Length Encoding Scheme and Its Application

Peng Wu,Shunping Zhou,Bo wan,Fang Fang,Sha Zhou 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.4

In this paper, we propose an improved two-dimensional run-length encoding (I2DRLE) scheme for representing grayscale images. Conventional 2D run-length encoding scheme is simple and effective that has been widely used, while it is not suitable to represent non-block images. Our approach is a new data compression algorithm inspired by 2D run-length encoding and quadtree, which apply some predefined patterns to represent various data and can sharply reduce the number of blocks in image representation. Experimental results show that this method is an effective lossless grayscale image encoding scheme.

Magnetic Resonance Image Manifestations of the Atypical Meningioma

Wu, Qing-Wu,Yan, Rui-Fang,Li, Qiang,Hu, Ying,Zhou, Feng-Mei,Ren, Ji-Peng,Yang, Rui-Min,Zhang, Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Through retrospective analysis of 13 cases of magnetic resonance image (MRI) manifestations of atypical meningiomas confirmed by operation and pathology in the First Affiliated Hospital of Xinxiang Medical University, the objective of this study was to evaluate the diagnostic value of MRI in order to improve the accuracy rate of preoperative diagnosis. In this retrospective analysis of MRI findings for atypical meningiomas in First Affiliated Hospital of Xinxiang Medical University from January to July in 2012, the location, morphology and tumor signals and other tumor imaging characteristics were covered. In 13 cases of atypical meningioma patients of this group, most tumors were located at typical sites (10/13), mainly the falx cerebri, parasagittal, convexity, saddle area. Only two cases were at atypical locations, 1 in the cerebellar hemisphere and 1 in a lateral ventricle. Most of the tumors showed T1 and T2 isointensity signals, and necrosis, calcification, and peritumoral edema were always featured. DWI showed isointensity in 11 cases (11/13), and hyperintensity in 2. Some 9 cases had dural tail signs, 12 had accurate positioning (12/13), and 2 were postoperative recurrences. MRI has high value in the diagnosis of atypical meningiomas, with important roles in early clinical diagnosis, treatment and prognosis evaluation.

Comprehensive Bioinformation Analysis of the MRNA Profile of Fascin Knockdown in Esophageal Squamous Cell Carcinoma

Wu, Bing-Li,Luo, Lie-Wei,Li, Chun-Quan,Xie, Jian-Jun,Du, Ze-Peng,Wu, Jian-Yi,Zhang, Pi-Xian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: Fascin, an actin-bundling protein forming actin bundles including filopodia and stress fibers, is overexpressed in multiple human epithelial cancers including esophageal squamous cell carcinoma (ESCC). Previously we conducted a microarray experiment to analyze fascin knockdown by RNAi in ESCC. Method: In this study, the differentially expressed genes from mRNA expression profilomg of fascin knockdown were analyzed by multiple bioinformatics methods for a comprehensive understanding of the role of fascin. Results: Gene Ontology enrichment found terms associated with cytoskeleton organization, including cell adhesion, actin filament binding and actin cytoskeleton, which might be related to fascin function. Except GO categories, the differentially expressed genes were annotated by 45 functional categories from the Functional Annotation Chart of DAVID. Subpathway analysis showed thirty-nine pathways were disturbed by the differentially expressed genes, providing more detailed information than traditional pathway enrichment analysis. Two subpathways derivated from regulation of the actin cytoskeleton were shown. Promoter analysis results indicated distinguishing sequence patterns and transcription factors in response to the co-expression of downregulated or upregulated differentially expressed genes. MNB1A, c-ETS, GATA2 and Prrx2 potentially regulate the transcription of the downregulated gene set, while Arnt-Ahr, ZNF42, Ubx and TCF11-MafG might co-regulate the upregulated genes. Conclusions: This multiple bioinformatic analysis helps provide a comprehensive understanding of the roles of fascin after its knockdown in ESCC.

Segmentation of Leaf Images Based on the Active Contours

Wu Peng,Li Wenlin,Song Wenlong 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.6

Leaves contain important genetic information which can be used as a basis for the identification of plants. As a first step of modeling virtual three-dimensional plant, how to extract visual characteristic information form leaf images has great significance. We propose an optimized C-V model in this paper, which can detect objects in homogeneous regions of given leaf images and speed up running time. The new method combines local information with global information and optimizes the defect that SDF needs to be reconstructed partially so that the energy function is improved. Experimental results show that our algorithm can stop active contours on the correct boundary, get accurate image segmentation, and the speed is more than 1.5 times faster to C-V model.

A review of preparation techniques of porous ceramic membranes

Peng Wu,Yuezhong Xu,Zhenxing Huang,Jiachao Zhang 한양대학교 세라믹연구소 2015 Journal of Ceramic Processing Research Vol.16 No.1

Porous ceramic membranes with their various advantages, such as better thermal, chemical and mechanical resistance, controllable micro-structure and little pollution to our environment, have been attracting much attention in the scientific community recently. With the development of technology, lowering the cost of ceramic membranes and prepare membranes with high permeability, high selectivity is becoming more important. The advances in the past ten years in techniques are reviewed. Finally, prospect of ceramic membranes was predicted.

The Fungicide Propamocarb Increases Lignin by Activating the Phenylpropanoid Pathway in Cucumis sativus L

Peng Wu,Qian-qian Guo,Zhi-wei Qin 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.5

Pesticide residue often remains on the surface of cucumber fruits after application, but the metabolic pathway and genes involved in pesticide metabolism remain unclear. In this study, we employed whole transcriptional analysisusing a high-throughput tag-sequencing technique (Tag-seq) to identify the genes involved in cucumber’s metabolism of the fungicide propamocarb. Transcript abundance was investigated by analyzing gene expression profiles. Differential expression analysis revealed the up-regulation of 546 genes, and the down-regulation of 185 genes. Using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, genes identified from whole transcriptome analysis as related to the phenylpropanoid pathway were most significantly differentially expressed. Fourteen of these genes were up-regulated after treatment with propamocarb. Further analysis revealed that these genes encoded six enzymes: phenylalanine ammonia-lyase (PAL), cytochrome P450 (CYP), AMP-dependent CoA ligase (AMP), anthranilate Nbenzoyltransferase protein (ANP), UDP-glucosyl transferase family protein (UDP), and peroxidase (POD). Compared to the control, the activities of PAL and POD were significantly increased (p < 0.01) after treatment with propamocarb, as was lignin synthesis such that acetylbromide-extractable lignins were increased by 41.1% (p < 0.05). Based on these results, we propose that propamocarb up-regulates the expression of genes involved in the phenylpropanoid pathway, leading to induction of lignin synthesis, which in turn triggers defense mechanisms in cucumber.

Research on Three Dimensional Reconstruction of Plant Root Based on spatial Geometry Structure and Morphology Architecture Parameters

Wu Peng,Wang Bao-kun,Leng Xin,Xie Peng-cheng 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.6

The root plays an important role in the ecosystem of plants. In order to solve the problem that to measure the root is difficult, this article proposes the three dimensional morphology model of plant root based on spatial geometry structure and morphology architecture parameters, and realize the three dimensional visualization of root. First, constructing three dimensional display model including root axis growing model, branch geometry model and root axis curve model; then combining topological structure of root, we confirm corresponding pixel, using morphology characteristic parameters outputted by root morphology to conduct the three dimensional reconstruction of root. Finally, we realize the visualization expression of plant root, basing on the VTK simulation software. The result of simulation, for establishing the whole visualization plant growing root further, lays technical foundation.