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Mustapha Goni Abatcha,Pei Ling Tan,Li- Oon Chuah,Gulam Rusul,S. R. Chandraprasad,Mohd. Esah Effarizah 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.8
The effectiveness of two different rapid methodsinvolving the 3MTM molecular detection assay Listeria andthe 3MTM Petrifilm environmental Listeria Plate wereevaluated for the rapid detection of Listeria from naturallycontaminated vegetables and chicken-processing environmentsagainst the standard culture-based method. A total of178 samples were examined for the presence of Listeria. Atotal of 47/178 (26.4%) by standard ISO culture-basedmethod (EN ISO 11290-1), 42/178 (23.6%) by 3MTMMDA Listeria and 40/178 (22.5%) by 3MTM Petrifilm ELPlate showed positive results, respectively. The accuracy,sensitivity, specificity, positive predictive value, and negativepredictive value for 3MTM MDA Listeria and 3MTMPetrifilm EL Plate were 97.2, 89.4, 99.3, 97.7, 96.4% and96.1, 85.1, 100.0, 100.0, 94.9%, respectively. Based on theCohen’s Kappa value, there was a complete and robustconcordance between 3MTM MDA Listeria (0.911) and3MTM Petrifilm EL Plates (0.894) as compared to thestandard culture-based method.
Cheng Han Ng,Kai En Chan,Yip Han Chin,Rebecca Wenling Zeng,Pei Chen Tsai,Wen Hui Lim,Darren Jun Hao Tan,Chin Meng Khoo,Lay Hoon Goh,Zheng Jye Ling,Anand Kulkarni,Lung-Yi Loey Mak,Daniel Q Huang,Mark C 대한간학회 2022 Clinical and Molecular Hepatology(대한간학회지) Vol.28 No.3
Background/Aims: Nonalcoholic fatty liver disease (NAFLD) is closely associated with diabetes. The cumulative impact of both diseases synergistically increases risk of adverse events. However, present population analysis is predominantly conducted with reference to non-NAFLD individuals and has not yet examined the impact of prediabetes. Hence, we sought to conduct a retrospective analysis on the impact of diabetic status in NAFLD patients, referencing non-diabetic NAFLD individuals. Methods: Data from the National Health and Nutrition Examination Survey 1999–2018 was used. Hepatic steatosis was defined with United States Fatty Liver Index (US-FLI) and FLI at a cut-off of 30 and 60 respectively, in absence of substantial alcohol use. A multivariate generalized linear model was used for risk ratios of binary outcomes while survival analysis was conducted with Cox regression and Fine Gray model for competing risk. Results: Of 32,234 patients, 28.92% were identified to have NAFLD. 36.04%, 38.32% and 25.63% were non-diabetic, prediabetic and diabetic respectively. Diabetic NAFLD significantly increased risk of cardiovascular disease (CVD), stroke, chronic kidney disease, all-cause and CVD mortality compared to non-diabetic NAFLD. However, prediabetic NAFLD only significantly increased the risk of CVD and did not result in a higher risk of mortality. Conclusions: Given the increased risk of adverse outcomes, this study highlights the importance of regular diabetes screening in NAFLD and adoption of prompt lifestyle modifications to reduce disease progression. Facing high cardiovascular burden, prediabetic and diabetic NAFLD individuals can benefit from early cardiovascular referrals to reduce risk of CVD events and mortality.