私立大學生의 成長發育 및 營養狀態에 關한 硏究 : 中學校 入試有無過程을 通한 比較

朴淳永,具燾書,朴良元,金振浩,南炳執,朴昌植,朴喆斌 慶熙大學校 1980 論文集 Vol.10 No.-

In order to ascertain any possible changes in the physical and the nutritional status of Korean high school students before and after the abolishment of college entrance examination system, an intensive survey was conducted on the physical conditions of the incoming freshmen students (9532 males and 3428 females) of Kyung Hee University from 1972 to 1980. The finding are as below. 1. Physical growth conditions In each of the average physical dimensions of body height, body weight, chest girth and sitting height, a remarkable improvement was recorded for all age groups after the matriculation was abolished. 2. Physical and nutritional indices Relative body weight showed constant values of 35.0 in male and 32.0 in female. Relative chest girth showed the normal chest girth style in all age groups of both sexes. Relative sitting height showed a constant value of 54 for both sexes. The values of vervaeck index of th nutritional status were shown to be between 86-87 in male and 83-94 in female, Pelidisi index 91 and 92-93, Rohrer index of physical status 121-125 and 130-132, and Kaup index 206-211 and 202-210, respectively.

Up-regulation of Heme Oxygenase-1 by Korean Red Ginseng Water Extract as a Cytoprotective Effect in Human Endothelial Cells

Yang, Ha-Na,Lee, Seung-Eun,Jeong, Seong-Il,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong-Seek The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.3

Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on $H_2O_2$-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.

Up-regulation of Heme Oxygenase-1 by Korean Red Ginseng Water Extract as a Cytoprotective Effect in Human Endothelial Cells

Hana Yang,Seung Eun Lee,Seong Il Jeong,Cheung-Seog Park,Young-Ho Jin,Yong Seek Park 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3

Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on H₂O₂-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.

고장초 추출물의 t-BHP로 산화적 손상이 유도된 HepG2 세포 보호 효과

박세호(Se-Ho Park),이재열(Jae-Yeul Lee),양선아(Seun-Ah Yang),방대석(Daesuk Bang),지광환(Kwang-Hwan Jhee) 한국생명과학회 2021 생명과학회지 Vol.31 No.3

고장초는 오랫동안 식용 및 의약 목적으로 차를 만드는 데 사용되어 왔다. 그러나 고장초를 고부가가치 식용재료로 사용하는 연구는 불충분하다. 이전 연구에서 우리는 tricin이 고장초의 주요 화합물임을 확인했다. 본 연구에서는 고장초 추출물의 HepG2 세포 보호 효과를 조사하였다. 고장초 추출물 또는 tricin의 HepG2 세포에 대한 독성 실험 결과, 사용한 모든 농도에서 고장초 추출물 또는 tricin에 대한 독성은 나타나지 않았다. 또한 tert-butyl hydroperoxide (t-BHP)에 의해 감소된 세포 생존율은 고장초 추출물(100 μg/ml에서 69.23%) 또는 tricin (100 ng/ml에서 74.47%)에 의해 증가되었다. 또한 고장초 추출물(250 μg/ml) 또는 tricin (250 ng/ml)은 산화적 손상에 의한 활성 산소종(ROS) 생성을 효과적으로 억제했다. 또한 항산화 효소로 알려진 superoxide dismutase 1(SOD1), catalase, heme oxygenase-1 (HO-1), nuclear factor erythroid-related factor 2 (Nrf2)의 단백질 발현은 고장초 추출물(100 μg/ml에서 2.1, 1.6, 1.7, 1.5배) 또는 tricin (250 ng/ml에서 2.6, 1.8, 2.2, 2.0배) 처리로 증가했으며, 이는 고장초 추출물의 HepG2 세포 보호 효과는 tricin의 활성과 관련이 있다는 것을 시사한다. 종합하면 고장초는 간 기능개선 관련 기능성 식품 소재로 개발이 가능할 것으로 기대된다. Zizania latifolia has long been used as a tea for both edible and medicinal purposes. However, research into the use of Z. latifolia as a high value-added edible material is lacking. In a previous study, we confirmed that tricin is the major component in Z. latifolia. In this study, we investigated the protective effect of a Z. latifolia extract (ZLE). Toxicity tests of ZLE or tricin on HepG2 cells revealed no toxicity due to ZLE or tricin at all concentrations used. The reduction in cell viability by tert-butyl hydroperoxide (t-BHP) was suppressed by treatment with ZLE or tricin. In addition, ZLE or tricin effectively inhibited the production of reactive oxygen species (generation of hydrogen peroxide, alkoxy free radicals, and peroxyl free radicals by t-BHP) and oxidative damage. ZLE or tricin treatments also increased the protein expression of superoxide dismutase 1 (SOD1), catalase, heme oxygenase-1 (HO-1), and nuclear factor erythroid-related factor 2 (Nrf2), which are known as antioxidant enzymes, suggesting that the protective effect of ZLE is related to activation of tricin. Taken together, the results indicate that Z. latifolia can be developed as a functional food material for improving liver function.

Eriodictyol Protects Endothelial Cells against Oxidative Stress-Induced Cell Death through Modulating ERK/Nrf2/ARE-Dependent Heme Oxygenase-1 Expression

Lee, Seung Eun,Yang, Hana,Son, Gun Woo,Park, Hye Rim,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong Seek MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.7

<P>The pathophysiology of cardiovascular diseases is complex and may involve oxidative stress-related pathways. Eriodictyol is a flavonoid present in citrus fruits that demonstrates anti-inflammatory, anti-cancer, neurotrophic, and antioxidant effects in a range of pathophysiological conditions including vascular diseases. Because oxidative stress plays a key role in the pathogenesis of cardiovascular disease, the present study was designed to verify whether eriodictyol has therapeutic potential. Upregulation of heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, in endothelial cells is considered to be helpful in cardiovascular disease. In this study, human umbilical vein endothelial cells (HUVECs) treated with eriodictyol showed the upregulation of HO-1 through extracellular-regulated kinase (ERK)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathways. Further, eriodictyol treatment provided protection against hydrogen peroxide-provoked cell death. This protective effect was eliminated by treatment with a specific inhibitor of HO-1 and RNA interference-mediated knockdown of HO-1 expression. These data demonstrate that eriodictyol induces ERK/Nrf2/ARE-mediated HO-1 upregulation in human endothelial cells, which is directly associated with its vascular protection against oxidative stress-related endothelial injury, and propose that targeting the upregulation of HO-1 is a promising approach for therapeutic intervention in cardiovascular disease.</P>

Crotonaldehyde-exposed macrophages induce heme oxygenase-1 expression as an adaptive mechanism

Seung Eun Lee,Hana Yang,Gun Woo Son,Hye Rim Park,Young-Ho Jin,Cheung-Seog Park,Yong Seek Park 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Cigarette smoke represents one of the most significant risk factors for the progression of vascular disease. Exposure to cigarette smoke induces oxidative stress in the vascular system and results in vascular dysfunction. Crotonaldehyde, a highly reactive α,β-unsaturated aldehyde, is a foremost compound of cigarette smoke and a product of endogenous lipid peroxidation. Heme oxygenase-1 (HO-1) expression plays an essential role in cellular defense against environmental insults and represents an adaptive response. Here, we showed the effects of crotonaldehyde on the induction of HO-1 expression in RAW 264.7 macrophages. Crotonaldehyde treatment resulted in significantly increased phosphorylation of p38 mitogenactivated protein kinase (MAPK) and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2). Furthermore, treatment with zinc protoporphyrin (ZnPP; the specific HO-1 inhibitor) markedly augmented the death of crotonaldehyde-treated macrophages. In summary, these results highlight the role of the HO-1 upregulation through p38 MAPK-Nrf2 activation in the adaptive response to crotonaldehyde in macrophages.

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

고정화 세포를 이용한 황화수소 제거공정 최적화

박양호,손효진,김경주,권규혁,이중헌 한국공업화학회 2003 응용화학 Vol.7 No.1

In this study, the hybitd reactor has used to remove H_2S from the toxic gas. Since the oxidation and reduction reactors were combined, theri sizes were important to stabilize developed process. The optimal sizes of oxidation and reduction reactors were determined considering iron oxidation rate and H_2S removal rate. The optimal ratio of reactor size was 4 L for oxidation reactor to 1 L for reduction reactor.Immobilized Thiobacillus ferrooxidans has been developed to minimize the size of oxidation reactor. Since the immobilized cell enhanced the oxidation rate, it was possible to incrase H_2S removal efficiency. It was possible to incerase operation time because immobilized cell can survie lger than free cell.

도시근교 자연휴양림의 이용객 특성과 이용행태 : 비슬산 자연휴양림을 사례로

박양춘,박순호,최정수 慶北大學校 社會科學大學 1997 社會科學 Vol.9 No.1

원래 자연휴양림은 대국민 휴양서비스의 제공과 산촌지역주민의 소득증대를 목표로 개발되었으나, 실제 개발방법이나 분위기가 일반 유원지 및 관광지과 뚜렷한 차별성을 찾아보기 어렵다. 이러한 문제점을 해결하기 위해서는 휴양림 이용객의 이용행태 및 만족도에 관한 연구가 전제된다. 따라서 본 연구에서는 비슬산 자연휴양림을 연구대상으로 이용객속성, 이용행태 그리고 만족도를 분석하여 개발방향을 제시하고자 한다. 비슬산 자연휴양림은 2계절형(여름ㆍ가을), 주말형, 당일이용형, 통과형 자연휴양림의 특성을 지니고 있다. 이러한 특성을 결정하는 가장 중요한 요소는 ‘접근성’이며, 이는 휴양림 이용객속성과 이용행태에 결정적인 영향을 미친다. 휴양림의 유인력을 높이기 위해서는 다양한 측면에서의 접근성 제고가 최우선과제이다. 그리고 기대도에 비해 만족도가 낮은 활동 및 시설의 보완 그리고 산촌지역주민들의 소득증대라는 측면에서의 임산물 생산기능과 관광기능을 연계하는 대책도 강구되어야 할 것이다. 결론적 편의시설 위주의 현 개발방식의 재검토가 요구된다. Recreation and leisure activities are recently increased and spread over all the social classes. Many people interest in natural recreation forest for recreation and leisure, and then the number of visitors is rapidly increased. There are few studies on the visitors' characteristics and behaviors. The purpose of this study was to analyze the socio-economic characteristics of visitors and their behaviors of Mt. Bisul natural recreation forest [BNRF], which is located in the close to a large city, Taegu. The data were collected by interviewing 59 visitors with a constructed questionnaire in BNRF. Like general natural recreation forest, BNRF is two season(summer, fall) and weekend type. In the socio-economic characteristics of visitors in BNRF, the noticeable characteristics of visitors were that the sex ratio was 1.17 and the high proportion of visitors of BNRF worked in professionㆍfreelancer and housewife. Most lodgers of BNRF were from Taegu and attracted by lodge itself. In terms of the visitors' behaviors in BNRF, the visit motivation in BNRF was accessibility and convenience of facilities. The informations were obtained by face-to-face. Most visitors were with their families and 3 to 9 persons. Most visitors traveled less than one hour to arrive in BNRF. The tour course of BNRF was mainly a piston type and a key type. The resource-used activities such as resting, walk, and talks with companions were preferred, and walk road and log cabin were most convenient. In the satisfaction for activities, most visitors of BNRF were satisfied with forest-recreational activities such as walkㆍforest-bathing, but not satisfied with sightseeing or exploring of adjacent resorts and forest products & local foods. This was resulted from no famous tourist resorts near BNRF. The level of satisfaction was more low than the level of expectation, generally. The gaps between two levels were considerable in watetplay and forest products. In BNRF the activities of waterplay and forest products should be developed to enhance the level of satisfaction. The studies on the visitors' characteristics and behaviors in the natural recreation forest are required to help develop natural recreation forest to compete with recreation resorts and make to improve the marketability. This study contributes to help make programs to attract more people to natural recreation forest and to improve the facilities that people want.