Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

마이크로파에 의한 Li₂O-2SiO₂글라스의 핵생성 및 결정 성장 거동

박희찬,박성수,김경태,김진남 釜山大學校生産技術硏究所 1998 生産技術硏究所論文集 Vol.55 No.-

Li₂O-SiO₂계 글라스를 재래식 열원과 마이크로파 열원으로 가열했을 때, 핵생성 및 결정화 거동을 비교 조사하였다. 전자주사현미경 분석, X선 회절 분석 및 시차열분석을 통하여 결과를 분석하였다. 재래식 열원과 마이크로파 열원으로 가열된 시편들의 핵생성 및 결정화 거동이 매우 상이하였다. 마이크로파가 시편내의 핵 생성을 억제시켰지만, 결정 성장은 축진시켰다고 추측된다. A comparison of nucleation and crystallization of Li₂O-SiO₂glass system under between the conventional and microwave heating was investigated. Scanning electron microscopy, X-ray diffractometry, and differential thermal analysis were used to evaluate the results. Nucleation and crystallization behaviors under the microwave heating appeared largely different from under the conventional heating. It was assumed that microwaves inhibitied nucleation, but accelerted crystal growth.

Boosting algorithm을 이용한 다중 질의 영상검색

박중수,엄경배 국립7개대학공동논문집간행위원회 2002 공업기술연구 Vol.2 No.-

Content-based image retrieval uses extracted image contents. But if the contents are consist of many features then the retrieval process may speed down. In this paper, we use boosting algorithm to reduce the redundancy of features. We experiment 286 wallpapers and get the successful result.

肝吸蟲症 濃厚流行地(金海市)에 있어서의 疫學的 推移에 관한 硏究

朴洙培,朱炅煥,林漢鍾 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

In order to observe the epidemiological changes of clonorchiasis in the high endemic area in Korea, the stool examinations were carried out in Bulam-dong, Kimhae City. A total of 423 stool specimens collected from the inhabitants of this area were examined both by cellophane thick smear method and Stoll’s egg dilution counting technique. The result of this survey was compared to the data obtained in 1973 by the quantitative analysis applying the regression equations, the simple and two-stage catalytic curves. The metacercarial density of Pseudorasbora parva and the tendency of familial aggregation of this area were also compared with those of 1973. In addition to this study informal interview were conducted to investigate the KAP (knowledge, attitude and practice) of the raw fish eating habit of the ingabitants in this area. The results are as follows: 1. Overall prevalence rate of clonorchiasis was reduced from 68.3% to 45.6% in a period of 10 years. 2. In 1973, both sexes in all age groups showed almost parallel high infection rates. However in 1983, the rate for males was slightly higher than that for females and a marked reduction in the prevalence was encountered in the youngest age group. 3. The mean E.P.G. count of Clonorchis sinensis infection in the surveyed area was 10,846 in 1973. But the mean E.P.G. count was decreased to 4,858 in 1983. The degree of intensity of Clonorchis sinensis infection determined by E.P.G. count was evenly distributed in geavy, very heavy, light and moderate infections in 1973 whereas an E.P.G. of over 10,000 was seen only in 14.0% of examinee in 1973. 4. The two-stage catalytic model was applied to this study and the calculation led to the equation y=1.042(e^(-0.003t)-e^(-0.074t)); a>b in 1973, and y-1.130(e^(-0.003t)-e^(-0.026t); a>b in 1983. 5. The intensity of endemicity was compared with the regression equations calculated from cumulative percentages of E.P.G. counts. The regression equations were y=2.75+1.46 log^(χ)(Cs. D_(50) 64.76) in 1973 compared to y=3.27+1.59 log^(χ)(Cs. D_(50) 12.25) in 1983. 6. In high endemic area such as Kimhae City the tendency of familial aggregation was recognized not in 1973 but in the present study. 7. Metacercarial infestation rate of P.parva did not change but metacercarial density was decreased from 1,312.4 ea/fish to 1,233.4 ea/fish in 1972 and 1973 to 160.8 ea/fish in 1983. 8. The KAP(knowledge, attitude and practice) of raw-fish eating habit of inhabitants were mentioned.

블록펄스 함수를 이용한 변압기의 여자돌입검출에 관한 연구

박철원,김수경,신명철 成均館大學校 科學技術硏究所 1996 論文集 Vol.47 No.1

In the differential protection scheme for large power transformer, it must restrain the tripping on magnetizing inrush condition. The conventional methods has used harmonic restraints by digital filters that include much computational burden and require the sampling rate to be an power of 2. This paper deals with detection scheme of the magnetizing inrush for power transformer by extract wanted frequency components using one of the orthogonal function sets called Block Pulse Function. The proposed method is tested using Salford EMTP output relaying signals of the model power system. The results are compared with those of algorithm based DFT, and then its method shows good response and speed.

Planar Magnetic 소자를 사용한 포워드 컨버터의 최적 설계

朴庚洙,成源基 三陟大學校 2000 論文集 Vol.33 No.1

This paper presented a design technique of the forward converter(30V, 5Vdc , 150W) using planar magnetic components is described. The application of planar magnetic components can solve the space problem which occurs when it is installed on PCB since planar transformer has low profile. In addition a lossless snubber circuit is added to reduce the device stress and to improve the system efficiency. The performance of designed system is verified by simulation and experiment by comparing the system using conventional magnetic components and the system using planar magnetic components.

HL-60 세포의 성장에 대한 제주자생 식물체의 효과

박수영,강희경 제주대학교 생명과학연구소 2000 제주생명과학연구 Vol.3 No.-

The purpose of the present study is to examine the effect of extracts of plants growing in Jeju on the growth of HL-60 leukemia cells. The effect was observed by measurement of metabolic activity and counting of viable cells. The methanol extracts of 17 plants growing in Jeju were screened for cytotoxic effect at the concentration of 100㎍/㎖. In result, the inhibitory effect on the growth was observed in the treatment of crude extracts of 6 plants. In particular, Thuja oriental is, Eurya emarginata, Mallotus japonic us, Viscum album markedly Inhibited the growth of HL-60 cells. The extracts of Viscum album, Thuja orientalis and Eurya emarginata induced DNA fragmentation in HL-60 cells. The results indicate that inhibitory effect of Viscum album, Thuja oriental is and Eurya emarginata on the growth of HL-60 seem to arise from the induction of apoptosis.

가와사끼 병 환아에서 이상뇌파와 신경학적 합병증 발현과의 연관성에 대한 고찰

박준수,공도연,박경배,민용식,김영창,정순이,이혜경 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

목적 : 가와사끼 병의 뇌파 소견 및 다른 임상인자들과의 연관성을 알아보고자 하였다. 방법 : 1994년 1월부터 1995년 12월 사이에 순천향대학 천안병원 소아과에 입원한 23명의 신경학적으로 정상인 가와사끼 병 환아에게 뇌파검사를 실시하여 이를 검사시기, 성별, 연령, 혈소판, 간기능 검사치, 그리고 동맥류 유무등의 임상인자들과 비교하였다. 결과 : 이상뇌파가 23명중 52.2%인 12명에게서 관찰되었으며, 이상뇌파로는 예파 및 극파, 극·서파 복합 양상과 전반적인 배경파의 서파양상이 나타났다. 뇌파의 검사시기가 진단부터 1주 이내인 경우에 이상뇌파의 빈도가 그 후보다 의미있게 높았으나, 그 밖의 다른 임상인자와의 연관성은 별 의미가 없었다. 결론 : 가와사끼 병 초기에 이상뇌파가 나타나는 것은 비록 신경적 증상은 없지만 중추 신경계가 영향을 받는다는 간접적인 증거로 생각할 수 있어 향후 신경적 합병증 발생 여부에 대한 면밀한 관찰이 필요하다고 생각된다. Purpose : This study is to find out if there is any association between EEG abnormalities in Kawasaki disease with other clinical factors. Methods : We performed EEG in 23 Kawasaki disease patients without any neurologic sign who admitted in Soonchunhyang Chunan Hospital from 1994. 1. to 1995. 12. and compared it with sex, age, platelet count, liver function test and presence of coronary artery aneurysm. Results : Abnormal EEG findings were observed in 12 of 23 patients(52.2%) and as for abnormal EEG findings, sharp, spike, spike & slow wave complex, and generalized slow background activity were noted. When EEG was performed within a week after the diagnosis, the incidence of abnormalities of EEG increased significantly but there was no correlation with other clinical factors. Conclusion : EEG abnormalities in the early stage of Kawasaki disease indicate that although there is no abnormal neurologic signs clinically, central nervous system might be affected and there should be a close observation for the developement of neurologic complication.

에어-오일 윤활에 의한 공작기계 주축계의 고속화연구

朴京鎬,崔大奉,金洙泰 國立 昌原大學校 産業技術硏究所 1992 産技硏論文集 Vol.6 No.-

According to the development of cutting tools, the high speed of machine tool's spindle is needed, and the demands for the precision machining, the improvement of light materials' productivity and etc. are increased. The spindle of machine tool with the maximum speed, ??·N, has lately been used and that over ??·N has been studied in advanced countries. In the country, the study on this field is needed because the spindle with the speed,??·N, is used up to now. Therefore, the study is carried out for the high speed spindle design, the lubrication system and the development of spindle unit.

원주지역에서 발생한 비외상성 심정지의 일주변화

박금수,김영식,이진응,임종천,이강현,임경수,황성오,최경훈 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

To estimate the quality of the emergency medical services system of Wonju City, we studied the diurnal variations of 179 non-traumatic cardiac arrest victims who received cardiopulmonary reuscitation at the emergency center of Wonju Christian Hospital. Diurnal variations of non-traumatic cardiac arrest patients were as follows ; The occurence of cardiac arrest at day-time was higher than night-time; 18 cases (11%) from midnight to AM 4, 25 cases (14%) from AM 4 to AM 8, 42 cases (24%) from AM 8 to AM 12, 46 cases (25%) from AM 12 to PM 4, 35 cases (19%) from PM 4 to PM 8, 13 cases (7%) from PM 8 to midnight. Witness cardiac arrest was increased more during the day than night ; 40% from midnight to AM 4, 48% from AM 4 to AM 8, 57% from AM 8 to AM 12.52% from AM 12 to PM 4, 60% from PM 4 to PM 8, 38% from PM 8 to midnight. The transportation time at night-time cardiac arrest was more longer than day-time cardiac arrest ; 30±12mins from midnight to AM 4, 26±9mins from AM 4 to AM 8, 27±12mins AM 8 to Am 12, 25±11mins from AM 12 to PM 4, 25±9mins from PM 4 to PM 8, 35±15mins from PM 8 to midnight. The rate of restoration of spontaneous circulation(ROSC) in day-time cardiac arrest was higher than the night-time cardiac arrest ; 30% from midnight to AM 4, 36% from AM 4 to AM 8, 32% AM 8 to AM 12, 44% from AM 12 to PM 4, 41% from PM 4 to PM 8, 15% from PM 8 to midnight. The survival rate of cardiac arrest has been correlated with collapse time, early bystander CPR, early advanced care. To improve outcome for prehospital cardiac arrest, we concluded that early bystander CPR, and early advanced life support should be performed at the scene and during the transportation especially at night.