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Naoki Nakamura,Osamu Takahashi,Minobu Kamo,Shogo Hatanaka,Haruna Endo,Norifumi Mizuno,Naoto Shikama,Mami Ogita,Kenji Sekiguchi 한국유방암학회 2014 Journal of breast cancer Vol.17 No.2
Purpose: The purpose of this study was to quantify the targetcoverage, homogeneity, and robustness of the dose distributionsagainst geometrical uncertainties associated with fourwhole breast radiotherapy techniques. Methods: The study wasbased on the planning-computed tomography-datasets of 20patients who underwent whole breast radiotherapy. A total offour treatment plans (wedge, field-in-field [FIF], hybrid intensitymodulatedradiotherapy [IMRT], and full IMRT) were created foreach patient. The hybrid IMRT plans comprised two opposedtangential open beams plus two IMRT beams. Setup errors weresimulated by moving the beam isocenters by 5 mm in the anterioror posterior direction. Results: With the original plan, thewedge technique yielded a high volume receiving ≥107% of theprescription dose (V107; 7.5%±4.2%), whereas the other threetechniques yielded excellent target coverage and homogeneity. A 5 mm anterior displacement caused a large and significant increasein the V107 (+5.2%±4.1%, p<0.01) with the FIF plan, butnot with the hybrid IMRT (+0.4%±1.2%, p=0.11) or full IMRT(+0.7%±1.8%, p=0.10) plan. A 5-mm posterior displacementcaused a large decrease in the V95 with the hybrid IMRT (-2.5%±3.7%, p<0.01) and full IMRT (-4.3%±5.1%, p<0.01) plans, butnot with the FIF plan (+0.1%±0.7%, p=0.74). The decrease inV95 was significantly smaller with the hybrid IMRT plan than withthe full IMRT plan (p<0.01). Conclusion: The FIF, hybrid IMRT,and full IMRT plans offered excellent target coverage and homogeneity. Hybrid IMRT provided better robustness against geometricaluncertainties than full IMRT, whereas FIF provided comparablerobustness to that of hybrid IMRT
Cloning of Agarase Gene from Non-Marine Agarolytic Bacterium Cellvibrio sp
( Ariga Osamu ),( Takayoshi Inoue ),( Hajime Kubo ),( Kimi Minami ),( Mitsuteru Nakamura ),( Michi Iwai ),( Hironori Moriyama ),( Mitsunori Yanagisawa ),( Kiyohiko Nakasaki ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
Agarase genes of non-marine agarolytic bacterium Cellvibrio sp. were cloned into Escherichia coli and one of the genes obtained using HindIII was sequenced. From nucleotide and putative amino acid sequences (713 aa, molecular mass; 78,771 Da) of the gene, designated as agarase AgaA, the gene was found to have closest homology to the Saccharophagus degradans (formerly, Microbulbifer degradans) 2-40 aga86 gene, belonging to glycoside hydrolase family 86 (GH86). The putative protein appears to be a non-secreted protein because of the absence of a signal sequence. The recombinant protein was purified with anion exchange and gel filtration columns after ammonium sulfate precipitation and the molecular mass (79 kDa) determined by SDS-PAGE and subsequent enzymography agreed with the estimated value, suggesting that the enzyme is monomeric. The optimal pH and temperature for enzymatic hydrolysis of agarose were 6.5 and 42.5ºC, and the enzyme was stable under 40ºC. LC-MS and NMR analyses revealed production of a neoagarobiose and a neoagarotetraose with a small amount of a neoagarohexaose during hydrolysis of agarose, indicating that the enzyme is a β-agarase.
Development of Intelligent Power Wheelchair Assisting for Frail Elderly People on Daily Life
Atsuhiro Nakamura,Gen Obayashi,Yasunari Fujimoto,Osamu Nitta,Toru Yamaguchi 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
Recently, the number of elderly people increases in Japan, and the development of welfare apparatus having the effect of minimizing care is required. In this research, authors develop Intelligent Power Wheelchair along with minimizing care needs for frail elderly people. This Intelligent Power Wheelchair features the four functions ; approach function by human gesture recognition, standing up assistance, walking assistance, and operation assistance by posture recognition. And it shows experiments which three assistances function in useful.
Dynamic Control Method of Explicit Address Mapping Table in IPv6 Single-Stack Network
Yasunobu Toyota,Osamu Nakamura 한국통신학회 2020 한국통신학회 APNOMS Vol.2020 No.09
In recent years, Internet Data Center (IDC) network operation with IPv6 single-stack gathers attention because of IPv4 address pool exhaustion and the high operational load associated with IPv4/IPv6 dual-stack. Since IPv4 traffic still accounts for a large percentage of the total Internet traffic, it is necessary to provide IPv4 services even in IPv6 single-stack networks. A method of providing IPv4 accessibility in an IPv6 single-stack network is known as IPv4 as a Service (IPv4aaS); there are three possible approaches. This research proves that IPv4/IPv6 translation is the most compatible and effective for IDC network operations. The SIIT-DC network design has been standardized as a way to provide IPv4 services with IPv4 / IPv6 Translation. In SIIT-DC, a translation node referred to as Border Relay (BR) is connected between the IPv4 Internet and an IPv6 IDC network in order to translate IPv4 and IPv6 mutually (in order to enable IPv4 service provision in IPv6 server). However, to operate an SIIT-DC network without the lack of redundancy and operational load spikes in multi-homing networks, the Explicit Address Mapping Table (EAMT) must be shared dynamically and consistently with BRs. This paper compares approaches to control EAMT dynamically in SIIT-DC environment and clarifies the benefits and drawbacks of each. The results are expected to contribute to the design of IPv6 single-stack networks in IDC.
관내 예혼함 화염에서 외부 CO₂ 레이저 조사에 의한 화염면 진동의 동적거동
박준성(June Sung Park),Fujita Osamu,Nakamura Yuji,Ito Hiroyuki 한국연소학회 2009 KOSCOSYMPOSIUM논문집 Vol.- No.39
Experiments in premixed C₂H₄/CO₂-O₂ flames (Le < 1) in a tube have been conducted, the mixture was ignited at the top, open end of and propagating downwardly toward the closed end. to investigate the exact motion of flame tip fluctuations at the initial moment of irradiating CO₂ laser, the completely flat flame front is selected as a default flame, which is corresponding to the primary acoustic instability as reported by Searby.[1] During the laser exposure to the unburned mixture, the flame fronts had extremely unstable behaviors, resulting in a strong turbulent flame eventually through the secondary acoustic instability. To elucidate the effect of flame curvatures, the flame velocity and curvature prior to the secondary acoustic instability were analyzed by using captured high speed camera images. the results showed that a sudden acceleration at advancing waves was observed. And then, there was the time lag between the flame velocity and curvature, indicating the increase in the flame velocity even with decreasing flame curvature. these behaviors are closely relevant not only to the diffusive-thermal effects but also to the acoustic waves. the plausible mechanism was proposed in this paper based on the acoustic influence.
Tatematsu, Hidezumi,Miyahara, Ryoji,Shimoyama, Yoshie,Funasaka, Kohei,Ohno, Eizaburou,Nakamura, Masanao,Kawashima, Hiroki,Itoh, Akihiro,Ohmiya, Naoki,Hirooka, Yoshiki,Watanabe, Osamu,Maeda, Osamu,Ando Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Background: A close association between patterns identified by magnifying narrow-band imaging (M-NBI) and histological type has been described. M-NBI patterns were also recently reported to be related to the mucin phenotype; however, detials remain unclear. Materials and Methods: We investigated the cellular differentiation of gastric cancer lesions, along with their mucosal distribution observed by M-NBI. Ninety-seven depressed-type early gastric cancer lesions (74 differentiated and 23 undifferentiated adenocarcinomas) were visualized by M-NBI. Findings were divided into 4 patterns based on abnormal microvascular architecture: a chain loop pattern (CLP), a fine network pattern (FNP), a corkscrew pattern (CSP), and an unclassified pattern. Mucin phenotypes were judged as gastric (G-type), intestinal (I-type), mixed gastric and intestinal (M-type), and null (N-type) based on 4 markers (MAC5AC, MUC6, MUC2, and CD10). The relationship of each pattern of microvascular architecture with organoid differentiation indicated by cancer cell differentiation and its distribution in each histological type of early gastric cancer was investigated. Results: All CLP and FNP lesions were differentiated. The cancer cell distribution showed organoid differentiation in 84.2% (16/19) and 61.1% (22/36) of the two types of lesions, respectively, and there was a significant difference from the unclassified pattern with organoid differentiation (p<0.001). Almost all (94.7%; 18/19) CSP lesions were undifferentiated, and organoid differentiation was observed in 72.2% (13/18). There was a significant difference from the unclassified pattern with organoid differentiation (p<0.05). Conclusions: Cellular differentiation and distribution are associated with microvascular architecture observed by M-NBI.