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냉적응 생쥐의대식세포 활성화에 미치는 PKC 억제제와 Ca^(2+) Antagonist의 영향
정노팔,박한우,최인호,오영근,신형철 大韓免疫學會 1995 大韓免疫學會誌 Vol.17 No.3
Human thymus 조직으로부터 cDNA library를 만들었다. 무작위로 선별한 cDNA clone중에서 468개 염기로 된 cDNA clone (PTH209)을 검색한 결과 124개의 아미노산을 code하는 EAP(Epstein-Barr asseciated protein) 유전자임을 확인하였다. Thymus의 EAP와 Epstein-Barr 바이러스 positive 및 negative cell line의 RNP에서 분리한 EAP^(13)를 비교한 결과, coding region은 동일하였으나 3'UT region은 달랐다. 더구나 EBERs(Epstein-Barr encoded RNAs) 와 La 자가항원이 결합된 RNP의 EAP와 마찬가지로, thymus의 EAP는 sea urchin의 developmental stage-specific 단백질로 알려진 217^(2) 과 매우 유사한 아미노산 서열을 보였다. 이런 결과로 미루어 thymus의 EAP는 thymic development에 중요한 역할을 하는 것으로 사료된다. To study the adaptation of immune systems in the animals kept in cold temperature, the recovery rate of peritoneal exudated mouse macrophage was measured in mouse bred at room temperature(RT) or at cold(S±2 C). NBT-reduction activities and phagocytic activities were measured too, to the macrophages recovered. The results are as follows; More than 40% of cells were recovered in the cold treated groups compaired to the RT bred groups. The NBT-reduction activities were totally blocked at 10mM EGTA, and were below 10% of the control in H7 100pM and TMB-8 0.1mM. The NBT-reduction activity of cold treated groups were higher than RT group at lower concentration than H7100pM and TMB-8 0.1mM . The H7 resulted no changes in phagocytic activities both cold and RT groups at 0.1-100pM. The TMB-8 resulted in significant decrease in phagocytic activities at 0.1 mM and 1mM. The conclusions are 1) the cold treated animals showed an increase in the NBT-reduction activities and in the macrophage recovery rates, 2) H7 was unable to block phagocytosis of yeasts up to 10011M concentration, 3) lectin-like yeast cell surface molecules have different signaling mechanism form IgG molecules.
Vibrio vulnificus균 분리를 위한 새로운 선택배지
박노춘,고광균 순천향대학교 1994 논문집 Vol.17 No.4
For rapid and accurate diagnosis of Vibrio vulnificus infection, selection of better culture media has been contineously pursued. Several kinds of media were developed for favorable growth conditions of V. vulnificus as well as more efficient differentiation of V. vulnificus from V. parahaemolyticus. These media which introduced in this report including TCBS-Cellobiose agar plate medium, BHI-Cellobiose agar plate medium, Yeast extract-Beef extract-Cellobiose agar plate medium and Meretrix lusoria-Cellobiose agar plate medium were compared in order to select the most efficient method for cultivation and differential identification of V. vulnificus and V. parahaemolyticus We cultivated the V. vulnificus and V. parahaemolyicus on the different kinds of media newly developed and the results were follows. 1. Yeast extract-Beef extract-Cellobiose agar medium is found to be the best for enrichment medium of V. vulnificus and also Meretrix lusoria-Cellobiose agar medium is seem to be comparable. 2. Yeast extract-Beef extract-Cellobiose with 0.8% oxgall and Meretrix lusoria-Cellobiose agar medium is appeared to suitable for differential medium of V. vulnificus. 3. TCBS-Cellobiose agar medium showed potential use of differential medium of V. vulnificus but the growth of V. vulnificus is slightly suppressed on this medium. 4. TCBS agar medium is not suitable for differential medium of V. vulnificus. 5. BHI-Cellobiose agar medium was not suitable for differential medium of V. vulnificus because of its suppression of the growth of V. vulnificus.
전리방사선이 Salmonella 균체 성분에 미치는 영향
이강순,박노춘 순천향대학교 1991 논문집 Vol.14 No.2
IN order to investigate the effect of gamma radiation on Salmonella typhi, Ty2, the components of amino acids, proteins, carbohydrates and lipids in irradiated cells were compared with those in unirradiated control cells respectively. The results obtained were as follows : 1. The inactivation curves of Salmonella typhi with Co? r-ray irradiation were exponential over a wide range to the irradiated doses, 2. Dose for the inactivation factor of 10? was 94.0 Krad in physiological saline or in phosphate buffered saline, 104.2 Krad in nutrient broth, 220.4 Krad in frozen state, 552.0 Krad in dried state, 88.3 Krad in the abundance of oxygen and 188.0 Krad in the deficience of oxygen, respectively. 3. Five consecutive irradiation of Salmonella typhi suspension at the does of 90 Krad gave no additional increase in resistance. 4. Even at the smallest does of 500 Krad, compositions of amono acids, proteins, carbohydrates, and lipids were more or less decreased and the distinct banding patterns were also lost decreased and the distinct banding patterns were also lost possibly due to degradation of the protein molecules.
Nou, V.,Inoue, H.,Lee, H.G.,Matsunaga, N.,Kuwayama, H.,Hidari, H. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.8
An increase in frequency of administration of exogenous growth hormone (GH) or GH-releasing hormone was reported to be a model to increase blood circulating insulin-like growth factor-1 (IGF-1) and to improve growth performance in animals. We have investigated the effect of twice daily administration of GH-releasing peptide-2 (GHRP-2) on growth performance, GH responsiveness and plasma insulin-like growth factor IGF-1 in swine. We administered to eight swine, 3 control and 5 treatment, a twice daily s.c. injections of GHRP-2 ($30{\mu}g/kg\;BW$) for a period of 10 days. Every day blood samples immediately taken before injections of GHRP-2 or saline, at 08:00 h and 16:00 h, were measured for IGF-1 concentrations. Blood samples for GH assay were collected every 20 min on days 1, 6 and 10, from 1 hour before and 3 h after GHRP-2 or saline injections at 08:00 h. GH peak concentrations and GH area under curve (GH AUC) on day 1, 6 and 10 in treatment group of swine were higher than those in control swine (p<0.05). Twice daily administration of GHRP-2 caused a significantly attenuation (p<0.05) of GH peak concentrations ($80.25{\pm}13.87$, $39.73{\pm}5.72$ and $27.57{\pm}6.06ng/ml$ for day 1, 6 and 10, respectively) and GH AUCs ($3,536.15{\pm}738.35$, $1,310.31{\pm}203.55$ and $934.37{\pm}208.99ng/ml$ for day 1, 6 and 10, respectively). However, there was no significant difference in GH peak concentration and GH AUC between day 6 and 10. Plasma IGF-1 concentration levels were higher in treatment than control group of swine (p<0.05) after 3 days of the treatment, and the levels reached a plateau from day 3 to 10 of experiment. Growth performance did not alter by GHRP-2 administration, even though a numerical increase of body weight gain and feed efficiency was observed. These results indicate that twice daily administration of GHRP-2 for 10 days in swine did not significantly influence on growth performance, caused an overall attenuation of GH response, and that elevation of plasma GH concentrations caused by GHRP-2 administration increased plasma IGF-1 concentrations, even though an attenuation of GH response was observed.
Nou, V.,Tomoshi, K.,Inoue, H.,Matsunaga, N.,Kuwayama, H.,Hidari, H. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.8
Negative feedback on GH responses to GH-releasing hormone (GHRH) and GH-releasing peptides (GHRPs) has been reported and this action has been suggested to act through an increase in somatostatin. To determine whether the acute administration of porcine GH (pGH) inhibits GH responsiveness to GHRP-2 and GHRH in swine, swine were intravenously administered with pGH (5${\mu}g$/kg BW) or placebo followed 180 min later by a second intravenous administration of saline, GHRP-2 (30 ${\mu}g$/kg BW), GHRH (1${\mu}g$/kg BW) and a combination of GHRP-2 and GHRH. Plasma GH concentration was measured by radioimmunoassay. Administration of pGH caused a significant increase in GH area under curve and GH peak concentrations (p<0.001) over placebo-treated group. Plasma GH concentrations peaked at 15 min and returned to baseline level within 90 min. Pretreatment of pGH abolished (p<0.01) GH response to GHRH and attenuated (p<0.05) GH response to GHRP-2 and GHRH combined, without affecting GH response to GHRP-2. These results demonstrate that negative feedback action on GH releasing effect of GHRH occurs in swine, and that GHRP-2 has ability to interact in this action.