Alternative Messenger RNA Splicing of Autophagic Gene Beclin 1 in Human B-cell Acute Lymphoblastic Leukemia Cells

Niu, Yu-Na,Liu, Qing-Qing,Zhang, Su-Ping,Yuan, Na,Cao, Yan,Cai, Jin-Yang,Lin, Wei-Wei,Xu, Fei,Wang, Zhi-Jian,Chen, Bo,Wang, Jian-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Beclin 1 is a key factor for initiation and regulation of autophagy, which is a cellular catabolic process involved in tumorigenesis. To investigate the role of alternative splicing of Beclin1 in the regulation of autophagy in leukemia cells, Beclin1 mRNA from 6 different types of cell lines and peripheral blood mononuclear cells from 2 healthy volunteers was reversely transcribed, subcloned, and screened for alternative splicing. New transcript variants were analyzed by DNA sequencing. A transcript variant of Beclin 1 gene carrying a deletion of exon 11, which encoded a C-terminal truncation of Beclin 1 isoform, was found. The alternative isoform was assessed by bioinformatics, immunoblotting and subcellular localization. The results showed that this variable transcript is generated by alternative 3' splicing, and its translational product displayed a reduced activity in induction of autophagy by starvation, indicating that the spliced isoform might function as a dominant negative modulator of autophagy. Our findings suggest that the alternative splicing of Beclin 1 might play important roles in leukemogenesis regulated by autophagy.

Detection and Monitoring of Potential Geological Disaster Using SBAS-InSAR Technology

Wei Niu,Xiaonong Hu,Bo Lin,Fanqi Meng,Yong Zhang,Jin Zhao 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.11

As a global geological environment problem, geological disasters are more likely to induce severe geological disasters and cause loss of personnel and property due to their suddenness and concealment, frequent heavy rainfall and regular extreme weather. In this paper, Sentinel-1A data and DEM data combined with short baseline synthetic aperture interferar (SBAS-InSAR) technology are used to analyze and calculate the spatial-temporal evolution characteristics of surface deformation in Zibo-Weifang area of Shandong Province during 2016-2019. Linear fitting of InSAR monitoring deformation results is performed using level monitoring data to verify the accuracy of the results. The findings indicate that surface deformation in most of the study area is relatively stable, but in some areas, especially near Lucun Town, the maximum negative deformation rate exceeds 80mm/a. Based on the surface deformation results, a total of 377 potential geological disasters are identified using an integrated multi-source stereo observation system of the sky and ground. This identification is done by observing optical remote sensing images and taking the absolute value of surface deformation rate greater than 10 mm/a as the judgment basis, along with ground investigation and review. Several typical geological disaster sites are screened and they are found to be in unstable state. The research results can provide scientific support for geological disaster prevention and control in Shandong Province.

Fermented cottonseed meal improves production performance and reduces fat deposition in broiler chickens

Niu Jun Li,Wei Lian Qing,Luo Yuan Qing,Yang Wen Ting,Lu Qi Cheng,Zheng Xin Xia,Niu Yu Jie,Sheng Wen,Cheng Hong,Zhang Wen Ju,Nie Cun Xi 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

Objective: This study was conducted to investigate the effect of fermented cottonseed meal (FCSM) on growth performance, carcass traits, and fat deposition in white-feather broiler chickens. Methods: A total of 480 male one-day-old white-feather broiler chickens were selected randomly and divided into four groups with six replicates of 20 chickens in each. The experimental chickens were fed diets including 3%, 6%, or 9% FCSM fermented by Candida tropicalis until 42 days old. In the experiment, the chickens of the control group were fed soybean meal. Results: FCSM supplementation linearly decreased the feed conversion ratio from d 15 to 21 and d 36 to 42, respectively (p<0.05). The percentage of carcass and semi-eviscerate increased in response to dietary FCSM supplementation at d 21 (p<0.05). The percentage of eviscerated and semi-eviscerate of 3FCSM was higher than that in other groups at d 35 (p<0.05). At the age of 42 d, the percentage of carcass increased in a quadratic way among increasing FCSM in diets (p<0.05). The subcutaneous fat thickness linearly decreased with the increasing levels of FCSM at d 21 (p<0.05). Gompertz and Logistic functions provided a better fit on abdominal fat and subcutaneous fat, respectively. The best fitted equation predicted that the maximum growth rate of abdominal fat weight and subcutaneous fat thickness occurred at d 28. FCSM had no significant effects on the shape of growth curve of abdominal fat weight and subcutaneous fat thickness, but reduced the height of the curve. Birds receiving the 6FCSM diet for 21 d had smaller adipocyte surface and lower serum glucose as well as triglyceride concentration. Conclusion: FCSM is beneficial for broiler chickens as it positively affects their growth and carcass in addition to altering their fat deposition. Objective: This study was conducted to investigate the effect of fermented cottonseed meal (FCSM) on growth performance, carcass traits, and fat deposition in white-feather broiler chickens.Methods: A total of 480 male one-day-old white-feather broiler chickens were selected randomly and divided into four groups with six replicates of 20 chickens in each. The experimental chickens were fed diets including 3%, 6%, or 9% FCSM fermented by <i>Candida tropicalis</i> until 42 days old. In the experiment, the chickens of the control group were fed soybean meal.Results: FCSM supplementation linearly decreased the feed conversion ratio from d 15 to 21 and d 36 to 42, respectively (p<0.05). The percentage of carcass and semi-eviscerate increased in response to dietary FCSM supplementation at d 21 (p<0.05). The percentage of eviscerated and semi-eviscerate of 3FCSM was higher than that in other groups at d 35 (p<0.05). At the age of 42 d, the percentage of carcass increased in a quadratic way among increasing FCSM in diets (p<0.05). The subcutaneous fat thickness linearly decreased with the increasing levels of FCSM at d 21 (p<0.05). Gompertz and Logistic functions provided a better fit on abdominal fat and subcutaneous fat, respectively. The best fitted equation predicted that the maximum growth rate of abdominal fat weight and subcutaneous fat thickness occurred at d 28. FCSM had no significant effects on the shape of growth curve of abdominal fat weight and subcutaneous fat thickness, but reduced the height of the curve. Birds receiving the 6FCSM diet for 21 d had smaller adipocyte surface and lower serum glucose as well as triglyceride concentration.Conclusion: FCSM is beneficial for broiler chickens as it positively affects their growth and carcass in addition to altering their fat deposition.

Coalition Formation Game Based Relay Selection and Frequency Sharing for Cooperative Relay Assisted Wireless D2D Networks with QoS Constraints

( Jinxin Niu ),( Wei Tang ),( Wei Guo ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.11

With device-to-device (D2D) communications, an inactive user terminal can be utilized as a relay node to support multi-hop communication so that connective experience of the cell-edge user as well as the capacity of the whole system can be significantly improved. In this paper, we investigate the spectrum sharing for a cooperative relay assisted D2D communication underlying a cellular network. We formulate a joint relay selection and channel assignment problem to maximize the throughput of the system while guaranteeing the quality of service (QoS) requirements of cellular users (CUs) and D2D users (DUs). By exploiting coalition formation game theory, we propose two algorithms to solve the problem. The first algorithm is designed based on merge and split rules while the second one is developed based on single user`s movement. Both of them are proved to be stable and convergent. Simulation results are presented to show the effectiveness of the proposed algorithms.

Reformability evaluation of blasting-enhanced permeability in in situ leaching mining of low-permeability sandstone-type uranium deposits

Wang Wei,Liang Xuanyu,Niu Qinghe,Wang Qizhi,Zhuo Jinyi,Su Xuebin,Zhou Genmao,Zhao Lixin,Yuan Wei,Chang Jiangfang,Zheng Yongxiang,Pan Jienan,Wang Zhenzhi,Ji Zhongmin 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.8

It is essential to evaluate the blasting-enhanced permeability (BEP) feasibility of a low-permeability sandstone-type uranium deposit. In this work, the mineral composition, reservoir physical properties and rock mechanical properties of samples from sandstone-type uranium deposits were first measured. Then, the reformability evaluation method was established by the analytic hierarchy process-entropy weight method (AHP-EWM) and the fuzzy mathematics method. Finally, evaluation results were verified by the split Hopkinson Pressure Bar (SHPB) experiment and permeability test. Results show that medium sandstone, argillaceous sandstone and siltstone exhibit excellent reformability, followed by coarse sandstone and fine sandstone, while the reformability of sandy mudstone is poor and is not able to accept BEP reservoir stimulation. The permeability improvement and the distribution of damage fractures before and after the SHPB experiment confirm the correctness of evaluation results. This research provides a reformability evaluation method for the BEP of the low-permeability sandstone-type uranium deposit, which contributes to the selection of the appropriate regional and stratigraphic horizon of the BEP and the enhanced ISL of the low-permeability sandstone-type uranium deposit.

Arge aurora Wei sp. nov. (Hymenoptera, Argidae) from China with a key to East Asian species of Arge nipponensis group

Wan Siying,Wu Duo,Niu Gengyun,Liu Ting,Wei Meicai 한국곤충학회 2022 Entomological Research Vol.52 No.1

Arge nipponensis group is proposed for 12 East Asian species. A key to species of this group is provided. Arge aurora Wei, sp. nov., a widespread species in China is described. It has been misidentified as Arge nipponensis Rohwer, 1910 in China since 1927. Arge nipponensis Rohwer is removed from the faunal list of China. The ovipositor and genitalia of A. nipponensis are described and figured for the first time. The geographic distribution analysis indicated that geographical barriers were probably the key to the distribution of A. aurora and A. nipponensis.

RBM24 exacerbates bladder cancer progression by forming a Runx1t1/TCF4/miR-625-5p feedback loop

Yin Yue-Wei,Liu Kai-Long,Lu Bao-Sai,Li Wei,Niu Ya-Lin,Zhao Chen-Ming,Yang Zhan,Guo Ping-Ying,Qi Jin-Chun 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

RNA–binding motif protein 24 (RBM24) acts as a multifunctional determinant of cell fate, proliferation, apoptosis, and differentiation during development by regulating premRNA splicing and mRNA stability. It is also implicated in carcinogenesis, but the functions of RBM24 in bladder cancer (BC) remain unclear. In the present study, we revealed that RBM24 was upregulated in BC tissues. Importantly, we found that a higher level of RBM24 was correlated with poor prognosis in BC patients. Overexpression of RBM24 promoted BC cell proliferation, while depletion of RBM24 inhibited BC cell proliferation in vivo and in vitro. Mechanistically, RBM24 positively regulated Runx1t1 expression in BC cells by binding to and enhancing Runx1t1 mRNA stability. Furthermore, Runx1t1 in turn promoted RBM24 expression by interacting with the transcription factor TCF4 and suppressing the transcription of miR-625-5p, which directly targets RBM24 and suppresses RBM24 expression. RBM24-regulated BC cell proliferation was moderated via the Runx1t1/TCF4/miR-625-5p feedback loop. These results indicate that the RBM24/Runx1t1/TCF4/miR-625-5p positive feedback loop participates in BC progression. Disruption of this pathway may be a potential therapeutic strategy for BC treatment.

Review of Leptocimbex formosanus group (Hymenoptera: Cimbicidae) with two new Chinese species

Yuchen Yan,Gengyun NIU,Bocheng LAN,Meicai Wei 한국곤충학회 2018 Entomological Research Vol.48 No.5

Leptocimbex formosanus group of Leptocimbex Semenov 1896, Cimbicidae is defined and the relationships and characteristics are provided. Two new species of this group are described from Hunan and Yunnan Provinces in China: Leptocimbex shinoharai Yan & Wei sp. nov. and L. naitoi Yan & Wei sp. nov. Redescriptions of the three known species of L. formosanus group (L. formosanus Enslin 1911, L. dendrobii Rohwer 1915 and L. nigropropodea Wei & Deng 2002) and a key to all known species of this group are provided.

Mean Square Detectability of LTI Systems over Finite-State Digital Block-Fading Channels

Wei Chen,Yuanyuan Zou,Nan Xiao,Yugang Niu 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.2

This paper studies the mean square quadratic (MSQ) detectability for multi-output networked systemsover finite-state digital block-fading channels. The packet-loss rate of each digital fading channel depends on thechannel power gain, as well as packet length and power level used for transmission. A finite-state random processis introduced to model time-varying fading channels, which characterizes various configurations of physical communicationenvironment and/or different channel fading amplitudes. Necessary and sufficient conditions for MSQdetectability over finite-state Markov digital block-fading channels are given in the form of algebraic Riccati equationsor linear matrix inequalities (LMIs). The estimation gain is given as a function of estimated/observed channelstate. In addition, explicit conditions on network for MSQ detectability over finite-state independent identicallydistributed (i.i.d.) digital block-fading channels are presented in terms of the unstable poles of the multi-outputplant. Finally, an application to Gilbert-Elliott channels (GECs) is provided to demonstrate the derived results.

A New Boiler-turbine-heating Coordinated Control Strategy to Improve the Operating Flexibility of CHP Units

Wei Wang,Guangming Zhang,Yuguang Niu,Zhenyu Chen,Peiran Xie,Zhe Chen 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.5

The operating flexibility of the power units is getting increasing attention from power systems especially those with large-scale fluctuating renewable energies. However, the combined heat and power (CHP) units are getting a bottleneck because their electricity productions are restricted by heat productions. This study aims to develop an electric-heat coordinated control strategy to make the CHP units more flexible. First of all, the dynamic model for a 300 MW CHP unit is set up, and its linear state-space description is obtained. A control strategy based on linear quadratic regulator (LQR) is then developed to satisfy different heat-power demands in various operating conditions. The control weights Q and R are optimized by particle swarm optimization. Moreover, the improved coordinated control strategy based on precise energy balance is put forward to increase the CHP power ramp rate considering electricity priority strategy and recovery control of the heat source. Finally, the simulation results show that the improved strategy is suitable for various CHP operating scenarios, and the case for electricity priority and heat recovery control significantly improves the unit power rate on the premise of stable heat supply. This work provides a reliable and flexible control mode for CHP units, which can support the power system stability and renewable energy integration.