BIOTIC CHANGES IN SOME AREAS OF EAST ASIA - ASSOCIATED RESPONSES TO NEOGENE EVOLUTION OF PACIFIC OCEAN GATEWAYS

( Wei Ning Wang ) 한국고생물학회 1997 Abstract volume Vol.- No.-

Correlation between the overexpression of epidermal growth factor receptor and mesenchymal makers in endometrial carcinoma

Wei-Ning Yang,Zhi-Hong Ai,Juan Wang,Yan-Li Xu,Yincheng Teng 대한부인종양학회 2014 Journal of Gynecologic Oncology Vol.25 No.1

Objective: The objective of this study was to evaluate the effect of overexpression of epidermal growth factor receptor (EGFR) on the expression of epithelial cell markers (E-cadherin and α-catenin) and mesenchymal cell markers (N-cadherin and vimentin) in endometrial carcinoma. Methods: The expression of all 4 markers was evaluated in EGFR overexpressing Ishikawa cells, control Ishikawa cells, and KLE cells using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. The expression of these 4 markers was also determined in cancerous tissues of patients with endometrial carcinoma using immunohistochemical staining. Results: Ishikawa cells transfected with EGFR showed decreased expression of E-cadherin and α-catenin and increased expression of N-cadherin and vimentin compared with control Ishikawa cells (p<0.01 for all). The expression of N-cadherin and vimentin was higher and the expression of E-cadherin and α-catenin was lower in stage II-III than stage I and in grade II-III than grade I endometrial carcinoma tissue (p<0.01 for all). Conclusion: Decreased expression of epithelial markers (E-cadherin and α-catenin) and increased expression of mesenchymal markers (N-cadherin and vimentin) were observed in human endometrial carcinoma tissue. These findings correlate with high EGFR expression in cultured endometrial carcinoma cells.

Research on an Information Application Integration Platform Based on SOA and Web Service

Ning Wei 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.12

The construction of enterprise information is facing more or less heterogeneous system integration problems in the deepening process. On the basis of analyzing the advantages of service oriented architecture (SOA) and Web service technology in realizing heterogeneous system integration, a fast and flexible enterprise information system integration scheme based on combining SOA and Web service technology is proposed to solve these problems of poor information sharing capability and business adaptability. This integration scheme uses the advantages of self described and contained application module and platform independence and programming language independence to implement the complex aggregation. The SOA is used to implement the standard and loosely coupled application architecture by using the characteristics of Web service. So this integration system provides a great solution to messaging communication with different enterprises or inter-enterprise. And it can effectively reduce the maintenance cost, shorten the adjustment time, improve the execution efficiency and make the enterprise with more competitive.

Catalysts for Cross-sector Collaboration in Disaster Response

Wei-Ning Wu 위기관리 이론과 실천 2017 위기관리 이론과 실천 세미나발표논문집 Vol.2018 No.-

Cross-sector collaboration has been considered a practical approach for enhancing the capacity of local governments to deliver public services and respond wicked problems of catastrophic events. Taiwan is prone to various natural and man-made disasters, and many countries have learned greatly from the emergency management experiences of Taiwan. While much has been written about the importance and functions of collaboration, very few has examined systematically what factors determine the effective collaboration in emergency response among different municipalities or various public agencies from the perspectives of emergency agencies. This study identifies factors that impact the effectiveness of collaborating emergency services with other public agencies in the local level of Taiwan. Using survey data from local fire branch heads, this research demonstrates that the ability of local fire branch heads to mobilize resources in emergency management process and the quality of citizen engagement in emergency exercise would influence the effective cross-sector collaboration in emergency response with other local fire branches.

Catalysts for Cross-sector Collaboration in Disaster Response : Evidence from Taiwan Local Emergency Management

Wei-Ning Wu 위기관리 이론과 실천 2017 위기관리 이론과 실천 학술대회 Vol.2017 No.12

Cross-sector collaboration has been considered a practical approach for enhancing the capacity of local governments to deliver public services and respond wicked problems of catastrophic events. Taiwan is prone to various natural and man-made disasters, and many countries have learned greatly from the emergency management experiences of Taiwan. While much has been written about the importance and functions of collaboration, very few has examined systematically what factors determine the effective collaboration in emergency response among different municipalities or various public agencies from the perspectives of emergency agencies. This study identifies factors that impact the effectiveness of collaborating emergency services with other public agencies in the local level of Taiwan. Using survey data from local fire branch heads, this research demonstrates that the ability of local fire branch heads to mobilize resources in emergency management process and the quality of citizen engagement in emergency exercise would influence the effective cross-sector collaboration in emergency response with other local fire branches.

Research on an Improved Multi-Population Ant Colony Optimization Algorithm and its Application

Ning Wei,Chunfeng Si 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.10

In allusion to the shortcomings of easy falling into the local optimization and difficult obtaining Pareto optimal solutions for the original ant colony optimization algorithm in solving the complex optimization problems, multi-population, parallel mechanism, dynamic evaporation strategy and chaos theory are introduced into the original ant colony optimization algorithm in order to propose an improved multi-population ant colony optimization(MPPDCACO) algorithm in this paper. In the proposed MPPDCACO algorithm, the ant colony is divided into scout ants, search ants and worker ants in order to make the ACO algorithm as far as possible to avoid falling into local optimization and improve the local search ability of ant colony. The multi-population parallel mechanism is used to exchange the information and improve the computational effectiveness. The dynamic evaporation strategy is used to dynamically adjust the evaporation coefficient of pheromone in order to improve the global search capability of the ACO algorithm. The chaos theory is used to realize the optimization search in order to obtain the pheromone distributing in choosing path process. So the proposed MPPDCACO algorithm can prevent the local convergence caused by the misbalance of pheromone and can improve the searching ability. In order to test the optimization performance of the proposed MPPDCACO algorithm, 6 traveling salesman problems are selected from the TSPLIB in here. The experimental results show that the proposed MPPACACO algorithm takes on better global searching ability and higher convergence speed.

Physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via regulating calcium signaling

( Ning Ding ),( Yanzhu Lu ),( Hanmin Cui ),( Qinyu Ma ),( Dongxia Qiu ),( Xueting Wei ),( Ce Dou ),( Ning Cao ) 생화학분자생물학회(구 한국생화학분자생물학회) 2020 BMB Reports Vol.53 No.3

We investigated the effects of physalin A, B, D, and F on osteoclastogenesis induced by receptor activator of nuclear factor кB ligand (RANKL). The biological functions of different physalins were first predicted using an in silico bioinformatic tool (BATMAN-TCM). Afterwards, we tested cell viability and cell apoptosis rate to analyze the cytotoxicity of different physalins. We analyzed the inhibitory effects of physalins on RANKL-induced osteoclastogenesis from mouse bone-marrow macrophages (BMMs) using a tartrate-resistant acid phosphatase (TRAP) stain. We found that physalin D has the best selectivity index (SI) among all analyzed physalins. We then confirmed the inhibitory effects of physalin D on osteoclast maturation and function by immunostaining of F-actin and a pit-formation assay. On the molecular level, physalin D attenuated RANKLevoked intracellular calcium ([Ca(2+)](i)) oscillation by inhibiting phosphorylation of phospholipase Cγ2 (PLCγ2) and thus blocked the downstream activation of Ca2+/calmodulindependent protein kinases (CaMK)IV and cAMP-responsive element-binding protein (CREB). An animal study showed that physalin D treatment rescues bone microarchitecture, prevents bone loss, and restores bone strength in a model of rapid bone loss induced by soluble RANKL. Taken together, these results suggest that physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via suppressing the PLCγ2-CaMK-CREB pathway. [BMB Reports 2020; 53(3): 154-159]

Wavelet-based automatic identification method of axle distribution information

Ning-Bo Wang,Wei-Xin Ren,Zhi-Wei Chen 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.63 No.6

Accurately extracting the axle distribution information of a passing vehicle from bridge dynamic responses experiences a key and challenging step in non-pavement bridge weigh-in-motion (BWIM). In this article, the wavelet transformation is adopted and the wavelet coefficient curve is used as a substitute for dynamic response. The driving frequency is introduced and expanded to multi-axle vehicle, and the wavelet coefficient curve on specific scale corresponding to the driving frequency is confirmed to contain obvious axle information. On this basis, an automatic method for axle distribution information identification is proposed. The specific wavelet scale can be obtained through iterative computing, and the false peaks due to bridge vibration can be eliminated through cross-correlation analysis of the wavelet coefficients of two measure points. The integrand function that corresponds to the maximum value of the cross-correlation function is used to identify the peaks caused by axles. A numerical application of the proposed axle information identification method is carried out. Numerical results demonstrate that this method acquires precise axle information from the responses of an axle-insensitive structure (e.g., girder) and decreases the requirement of sensitivity structure of BWIM. Finally, an experimental study on a full-scale simply supported bridge is also conducted to verify the effectiveness of this method.

Transverse Tensile Deformation and Failure of Three-dimensional Five-directional Braided Carbon Fiber Composites

Wei Zhou,Zhi-yuan Wei,Guang-fei Wang,Kang-ning Han,Ran Liu,Lian-Hua Ma 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4

Accurate characterisation of transverse tensile deformation and damage evolution is of importance for evaluatingthe failure behaviors of three-dimensional (3D) braided composites. In the present study, a finite element method (FEM) andseveral non-destructive testing methods including acoustic emission, digital image correlation, and infrared thermography aredeveloped to investigate the transverse tensile deformation and damage evolution of 3D five-directional braided composites. In the finite element approach, a matrix-impregnated fiber bundles (MIFB) model and a representative volume cell (RVC)model, which take into account the fiber bundles and matrix, are respectively established to predict the effective mechanicalproperties of fiber bundles and simulate the deformation and progressive damage of such composites. The damaged locationsand the failure modes including matrix crack, fiber debonding and shear fracture of fiber are predicted and verified byexperimental tests. The non-destructive tests show that the transverse tensile fracture process can be divided into four stageswhich correspond to acoustic emission signals severally. The combination of the FEM based numerical modeling andmultiple non-destructive characterisation methods can accurately monitor the deformation and damage behaviors of 3Dbraided composites under transverse tensile loads and thus provide a reference for structural health monitoring of compositesin practical application.

Structural characterization of the pulmonary innate immune protein SPLUNC1 and identification of lipid ligands

Ning, Fangkun,Wang, Chao,Berry, Karin Zemski,Kandasamy, Pitchaimani,Liu, Haolin,Murphy, Robert C.,Voelker, Dennis R.,Nho, Chu Won,Pan, Choel-Ho,Dai, Shaodong,Niu, Liwen,Chu, Hong-Wei,Zhang, Gongyi The Federation of American Societies for Experimen 2014 The FASEB Journal Vol.28 No.12

<P>The short palate, lung and nasal epithelial clone 1 (SPLUNC1) protein is a member of the palate, lung, and nasal epithelium clone (PLUNC) family, also known as bactericidal/permeability-increasing (BPI) fold-containing protein, family A, member 1 (BPIFA1). SPLUNC1 is an abundant protein in human airways, but its function remains poorly understood. The lipid ligands of SPLUNC1 as well as other PLUNC family members are largely unknown, although some reports provide evidence that lipopolysaccharide (LPS) could be a lipid ligand. Unlike previous hypotheses, we found significant structural differences between SPLUNC1 and BPI. Recombinant SPLUNC1 produced in HEK 293 cells harbored several molecular species of sphingomyelin and phosphatidylcholine as its ligands. Significantly, <I>in vitro</I> lipid-binding studies failed to demonstrate interactions between SPLUNC1 and LPS, lipoteichoic acid, or polymyxin B. Instead, one of the major and most important pulmonary surfactant phospholipids, dipalmitoylphosphatidylcholine (DPPC), bound to SPLUNC1 with high affinity and specificity. We found that SPLUNC1 could be the first protein receptor for DPPC. These discoveries provide insight into the specific determinants governing the interaction between SPLUNC1 and lipids and also shed light on novel functions that SPLUNC1 and other PLUNC family members perform in host defense.—Ning, F., Wang, C., Berry, K. Z., Kandasamy, P., Liu, H., Murphy, R. C., Voelker, D. R., Nho, C. W., Pan, C.-H., Dai, S., Niu, L., Chu, H.-W., Zhang, G. Structural characterization of the pulmonary innate immune protein SPLUNC1 and identification of lipid ligands.</P>