Modelling of beam-to-column connections at elevated temperature using the component method

N.H. Ramli Sulong,A.Y. Elghazouli,B.A. Izzuddin,N. Ajit 국제구조공학회 2010 Steel and Composite Structures, An International J Vol.10 No.1

In this paper, a nonlinear model is developed using the component method in order to represent the response of steel connections under various loading conditions and temperature variations. The model is capable of depicting the behaviour of a number of typical connection types including endplate forms (extended and flush) and angle configurations (double web, top and seat, and combined top-seat-web) in both steel and composite framed structures. The implementation is undertaken within the finite element program ADAPTIC, which accounts for material and geometric nonlinearities. Verification of the proposed connection model is carried out by comparing analytical simulations with available results of isolated joint tests for the ambient case, and isolated joint as well as sub-frame tests for elevated temperature conditions. The findings illustrate the reliability and efficiency of the proposed model in capturing the stiffness and strength properties of connections, hence highlighting the adequacy of the component approach in simulating the overall joint behaviour at elevated temperature.

Taxonomic description and genome sequence of Halobacillus marinus sp. nov., a novel strain isolated from Chilika Lake, India

Ananta N. Panda,Samir Ranjan Mishra,Lopamudra Ray,Surajit Das,Gurdeep Rastogi,Ajit Kumar Pattanaik,Tapan Kumar Adhya,Mrutyunjay Suar,Vishakha Raina 한국미생물학회 2018 The journal of microbiology Vol.56 No.4

A moderately halophilic spore forming, motile, Gram-positive, rod-shaped bacterial strain designated as KGW1T was isolated from water sample of Chilika Lake and characterized taxonomically using polyphasic approach. The strain grew in the presence of 0–25% (w/v) NaCl in marine salt agar media, hydrolyzes casein, and gelatin and shows presence of alkaline proteases. The major cell wall menaquinone was MK7 and major cellular fatty acids were anteiso-C15:0 (44.89%), anteiso-C17:0 (6.18%), isoC15:0 (19.38%), and iso-C16:0 (7.39%). Several chemotaxonomic features conform the isolate be a member of genus Halobacillus. The isolate KGW1T contained A1γ meso-Dpm-direct type of peptidoglycan which is different from its phylogenetically closest neighbours. The 16S rRNA gene sequence based phylogenetic analysis also revealed the strain KGW1T was affiliated to the genus Halobacillus and sequence similarity between the isolated strain and the type strains of Halobacillus species were found closest to, H. dabanensis D-8 DSM 18199T (99.08%) and H. faecis IGA7-4 DSM 21559T (99.01%), H. trueperi SL-5 DSM 10404T (98.94%). The in silico DDH showed that the values in a range of 14.2–17.5% with the most closest strain H. dabanensis D-8 DSM 18199T and other type strains of the genus Halobacillus for which whole genome sequence is reported. DNA-DNA relatedness between strain KGW1T and the closest type strain Halobacillus trueperi DSM 10404T was 11.75% (± 1.15). The draft genome sequence includes 3,683,819 bases and comprises of 3898 predicted coding sequences with a G + C content of 46.98%. Thus, the significant distinctiveness supported by phenotypic and genotypic data with its closest neighbors and other closely related species confirm the strain KGW1T to be classified as a novel species within the genus Halobacillus, for which the name Halobacillus marinus sp. nov. is proposed. The type strain is KGW1T (= DSM 29522 = JCM 30443).

Synthesis and Evaluation of New Generation Cross-Bridged Bifunctional Chelator for ⁶⁴Cu Radiotracers

Dale, Ajit V.,An, Gwang Il,Pandya, Darpan N.,Ha, Yeong Su,Bhatt, Nikunj,Soni, Nisarg,Lee, Hochun,Ahn, Heesu,Sarkar, Swarbhanu,Lee, Woonghee,Huynh, Phuong Tu,Kim, Jung Young,Gwon, Mi-Ri,Kim, Sung Hong American Chemical Society 2015 Inorganic Chemistry Vol.54 No.17

<P>Bifunctional chelators have been successfully used to construct Cu-64-labeled radiopharmaceuticals. Previously reported chelators with cross-bridged cyclam backbones have various essential features such as high stability of the copper(II) complex, high efficiency of radiolabeling at room temperature, and good biological inertness of the radiolabeled complex, along with rapid body clearance. Here, we report a new generation propylene-cross-bridged chelator with hybrid acetate/phosphonate pendant groups (PCB-TE1A1P) developed with the aim of combining these key properties in a single chelator. The PCB-TE1A1P was synthesized from cyclam with good overall yield. The Cu(II) complex of our chelator showed good robustness in kinetic stability evaluation experiments, such as acidic decomplexation and cyclic voltammetry studies. The Cu(II) complex of PCB-TE1A1P remained intact under highly acidic conditions (12 M HC1, 90 degrees C) for 8 d and showed quasi-reversible reduction/oxidation peaks at -0.77 V in electrochemical studies. PCB-TE1A1P was successfully radiolabeled with Cu-66 ions in an acetate buffer at 60 degrees C within 60 min. The electrophoresis study revealed that the Cu-64-PCB-TE1A1P complex has net negative charge in aqueous solution. The biodistribution and in vivo stability study profiles of 64Cu-PCB-TE1A1P indicated that the radioactive complex was stable under physiological conditions and cleared rapidly from the body. A whole body positron emission tomography (PET) imaging study further confirmed high in vivo stability and fast clearance of the complex in mouse models. In conclusion, PCB-TE1A1P has good potential as a bifunctional chelator for 'Cu-based radiopharmaceuticals, especially those involving peptides.</P>

New macrobicyclic chelator for the development of ultrastable 64Cu-radiolabeled bioconjugate.

Pandya, Darpan N,Dale, Ajit V,Kim, Jung Young,Lee, Hochun,Ha, Yeong Su,An, Gwang Il,Yoo, Jeongsoo American Chemical Society 2012 Bioconjugate chemistry Vol.23 No.3

<P>Ethylene cross-bridged cyclam with two acetate pendant arms, ECB-TE2A, is known to form the most kinetically stable (64)Cu complexes. However, its usefulness as a bifunctional chelator is limited because of its harsh radiolabeling conditions. Herein, we report new cross-bridged cyclam chelator for the development of ultrastable (64)Cu-radiolabeled bioconjugates. Propylene cross-bridged TE2A (PCB-TE2A) was successfully synthesized in an efficient way. The Cu(II) complex of PCB-TE2A exhibited much higher kinetic stability than ECB-TE2A in acid decomplexation studies, and also showed high resistance to reduction-mediated demetalation. Furthermore, the quantitative radiolabeling of PCB-TE2A with (64)Cu was achieved under milder conditions compared to ECB-TE2A. Biodistribution studies strongly indicate that the (64)Cu complexes of PCB-TE2A cleared out rapidly from the body with minimum decomplexation.</P>

Modelling of beam-to-column connections at elevated temperature using the component method

B.A. Izzuddin,N.H. Ramli Sulong,A.Y. Elghazouli,N. Ajit 국제구조공학회 2006 Steel and Composite Structures, An International J Vol.6 No.1

In this paper, a nonlinear model is developed using the component method in order to represent the response of steel connections under various loading conditions and temperature variations. The model is capable of depicting the behaviour of a number of typical connection types including endplate forms (extended and flush) and angle configurations (double web, top and seat, and combined top-seat-web) in both steel and composite framed structures. The implementation is undertaken within the finite element program ADAPTIC, which accounts for material and geometric nonlinearities. Verification of the proposed connection model is carried out by comparing analytical simulations with available results of isolated joint tests for the ambient case, and isolated joint as well as sub-frame tests for elevated temperature conditions. The findings illustrate the reliability and efficiency of the proposed model in capturing the stiffness and strength properties of connections, hence highlighting the adequacy of the component approach in simulating the overall joint behaviour at elevated temperature.

Removal of Cu(II) by biosorption onto coconut shell in fixed-bed column systems

Mike A. Acheampong,Kannan Pakshirajan,Ajit P. Annachhatre,Piet N.L. Lens 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.3

Biosorption of Cu(II) onto coconut shell, an agricultural biomaterial, was studied in a fixed-bed column. The Cu(II) biosorption column had the best performance at 10 mg Lˉ1 inlet Cu(II) concentration, 10 mL minˉ1 flow rate and 20 cm bed depth. The equilibrium uptake of Cu(II) amounted to 7.25 mg gˉ1. The simulation of the breakthrough curve was successful with the BDST and Yoon–Nelson models, but the entire breakthrough curve was best predicted by the Clark model. The design of a fixed bed column for Cu(II)removal from wastewater by biosorption onto coconut shell can be done based on these models.